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The map label for this gene is fliA [H]

Identifier: 119775415

GI number: 119775415

Start: 2745826

End: 2746545

Strand: Reverse

Name: fliA [H]

Synonym: Sama_2280

Alternate gene names: 119775415

Gene position: 2746545-2745826 (Counterclockwise)

Preceding gene: 119775416

Following gene: 119775414

Centisome position: 63.78

GC content: 49.86

Gene sequence:

>720_bases
GTGAATAAAGCCGCGGCGTATACCTCCATGGAGAGTAAAACCTCCATCGTTGAACAGTACGCACCGCTGGTTAAAAGAAT
CGCGCACCATATGTTGGCCAGATTGCCGGCATCCGTACAGTTGGACGACTTGCTGCAGGCGGGCATGATTGGCCTGCTTG
AGGCATCGGCCAAATTTGACGGCGGCAAAGGCGCCAAGTTTGAAACCTTTGCTGGCATTCGTATTCGTGGTGCCATGTTG
GATGAAATCCGTAAAGGTGATTGGGTTCCACGTTCTGTTCACCGTAATCAGCGCAGAGTCGCCCAGGTGATAGACGAACT
GGAACAAGAGCTCGGACGTGATGCCCGGGACACAGAAATTGCCGAAAGACTTGATATGTCGATGGAGGAATACCATCATA
TCCTCAATGATGTTTCTGTTGGTAAAATCATAGGGATAGAAGATTTAGGCGTATCACATGATGTTCTGGTTCCCGACGGG
GAGCAAACCAACGACATTTATGATGACCTGGCTCAGAGCCAGTTCCAGCAGGCCTTGGCAGATGCCATTCGCCAGTTACC
CGAGCGGGACGCGCTGGTGTTATCACTTTATTACGATGAAGCACTGAATTTAAAAGAAATAGGCGCCATTCTTGATGTGA
GTGAGTCGCGGGTATGCCAGATCCACAGTCAGGCTATGCTCAGGCTCAAAGGTAAGCTCAAGCATTGGACACAAGTATAA

Upstream 100 bases:

>100_bases
AAGGTGATGAGCTGGCCTATCCCGCAGCAACCCGGTGGCCATCTCGAATTTTTCGTAGAGCGTCTGGTACATCGTCCCGA
TTATCAAGAGGACAGAGCGA

Downstream 100 bases:

>100_bases
ATTATTATTTGAGCAGCTAGAGTGTCAGCTCACCGGAGGAAACCTTGGACAAGAATATGAAGATTCTCATTGTTGACGAC
TTTTCAACAATGAGACGTAT

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNKAAAYTSMESKTSIVEQYAPLVKRIAHHMLARLPASVQLDDLLQAGMIGLLEASAKFDGGKGAKFETFAGIRIRGAML
DEIRKGDWVPRSVHRNQRRVAQVIDELEQELGRDARDTEIAERLDMSMEEYHHILNDVSVGKIIGIEDLGVSHDVLVPDG
EQTNDIYDDLAQSQFQQALADAIRQLPERDALVLSLYYDEALNLKEIGAILDVSESRVCQIHSQAMLRLKGKLKHWTQV

Sequences:

>Translated_239_residues
MNKAAAYTSMESKTSIVEQYAPLVKRIAHHMLARLPASVQLDDLLQAGMIGLLEASAKFDGGKGAKFETFAGIRIRGAML
DEIRKGDWVPRSVHRNQRRVAQVIDELEQELGRDARDTEIAERLDMSMEEYHHILNDVSVGKIIGIEDLGVSHDVLVPDG
EQTNDIYDDLAQSQFQQALADAIRQLPERDALVLSLYYDEALNLKEIGAILDVSESRVCQIHSQAMLRLKGKLKHWTQV
>Mature_239_residues
MNKAAAYTSMESKTSIVEQYAPLVKRIAHHMLARLPASVQLDDLLQAGMIGLLEASAKFDGGKGAKFETFAGIRIRGAML
DEIRKGDWVPRSVHRNQRRVAQVIDELEQELGRDARDTEIAERLDMSMEEYHHILNDVSVGKIIGIEDLGVSHDVLVPDG
EQTNDIYDDLAQSQFQQALADAIRQLPERDALVLSLYYDEALNLKEIGAILDVSESRVCQIHSQAMLRLKGKLKHWTQV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=236, Percent_Identity=50, Blast_Score=218, Evalue=2e-58,
Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=26.1603375527426, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26802; Mature: 26802

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAAAYTSMESKTSIVEQYAPLVKRIAHHMLARLPASVQLDDLLQAGMIGLLEASAKFD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
GGKGAKFETFAGIRIRGAMLDEIRKGDWVPRSVHRNQRRVAQVIDELEQELGRDARDTEI
CCCCCCEEHCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AERLDMSMEEYHHILNDVSVGKIIGIEDLGVSHDVLVPDGEQTNDIYDDLAQSQFQQALA
HHHHCCCHHHHHHHHHCCCCCCEECHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHH
DAIRQLPERDALVLSLYYDEALNLKEIGAILDVSESRVCQIHSQAMLRLKGKLKHWTQV
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKAAAYTSMESKTSIVEQYAPLVKRIAHHMLARLPASVQLDDLLQAGMIGLLEASAKFD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
GGKGAKFETFAGIRIRGAMLDEIRKGDWVPRSVHRNQRRVAQVIDELEQELGRDARDTEI
CCCCCCEEHCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AERLDMSMEEYHHILNDVSVGKIIGIEDLGVSHDVLVPDGEQTNDIYDDLAQSQFQQALA
HHHHCCCHHHHHHHHHCCCCCCEECHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHH
DAIRQLPERDALVLSLYYDEALNLKEIGAILDVSESRVCQIHSQAMLRLKGKLKHWTQV
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]