BacMap

Definition	Shewanella amazonensis SB2B chromosome, complete genome.
Accession	NC_008700
Length	4,306,142

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The map label for this gene is phaB [H]

Identifier: 119775385

GI number: 119775385

Start: 2713804

End: 2714535

Strand: Reverse

Name: phaB [H]

Synonym: Sama_2250

Alternate gene names: 119775385

Gene position: 2714535-2713804 (Counterclockwise)

Preceding gene: 119775386

Following gene: 119775384

Centisome position: 63.04

GC content: 39.07

Gene sequence:

>732_bases
ATGAATACTAAAGCAGTCGTAGTTACAGGTGCCACAGGAGGAATTGGTTGGACTATTGCCGAATACCTAATGTCTCTTGA
ATATTTCGTTATCGGCACTACCCATCGTGCTCCTCAATCAGCCTGTGAACAATGGATAGAAATGGCAGATAAGAGAGGAG
TGTTAATTAAATACGACTCTAACGTTGACAACCTTTCAATAGATACGTTAATTGAGGTTGCTAACGGCCACTCTATTTAT
GGATTAGTTAATAATTTGGGGATCACACGAGATTCATCTTTTAAAAAGATGACTTCTGAACAGTGGAAACAAGTTATGGA
TGTCAACTTGTTTTCCGTTTTTTCTTTAACTCAATGCATTTTTAATCATATGGCTTTGTCAGGAAGTGGTAGAATCGTTA
ATATTTCTTCGGTTAATGCACATAGAGGTCAGTTTGGTCAGGTTAATTATTGTGCGGCTAAAGCTGGCTTGTTGGGTTTT
ACAAAGGCGCTTTCACTAGAGGGCGCTAAGTCTGGCATTACTGTGAATAGCGTTTCACCTGGATATACTGATACTAAAAT
GCTCTCGCATATTCCAACAGATATTCTGCAGTCAATAAAAGATTCAGTTCCGATGAAGCGCTTAGCCAAACCTATCGAAA
TTGCACATGCAGTTGCATTTTTGTTAGACGATAAGTCAGCTTATATCACAGGAGCGGACATTCCTGTGAATGGTGGATTG
CATTTATCATGA

Upstream 100 bases:

>100_bases
GAAGTGATAGCTTAATAGGAATAGGTGCTGTAGTGACTAAAAACATAGACTCCAATGTAATCGCGGCAGGGAATCCATCA
AAGATTTTAAGGAGTAATAA

Downstream 100 bases:

>100_bases
AAGTTCTGATTACAGGTGGTTCAGGATTTGTCGGAAGGTATTTGCAAGGATATTTTAATTCGGATGAATACGATGTCGCA
ATAACTAGCAGGTGTAAATC

Product: acetoacetyl-CoA reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNTKAVVVTGATGGIGWTIAEYLMSLEYFVIGTTHRAPQSACEQWIEMADKRGVLIKYDSNVDNLSIDTLIEVANGHSIY
GLVNNLGITRDSSFKKMTSEQWKQVMDVNLFSVFSLTQCIFNHMALSGSGRIVNISSVNAHRGQFGQVNYCAAKAGLLGF
TKALSLEGAKSGITVNSVSPGYTDTKMLSHIPTDILQSIKDSVPMKRLAKPIEIAHAVAFLLDDKSAYITGADIPVNGGL
HLS

Sequences:

>Translated_243_residues
MNTKAVVVTGATGGIGWTIAEYLMSLEYFVIGTTHRAPQSACEQWIEMADKRGVLIKYDSNVDNLSIDTLIEVANGHSIY
GLVNNLGITRDSSFKKMTSEQWKQVMDVNLFSVFSLTQCIFNHMALSGSGRIVNISSVNAHRGQFGQVNYCAAKAGLLGF
TKALSLEGAKSGITVNSVSPGYTDTKMLSHIPTDILQSIKDSVPMKRLAKPIEIAHAVAFLLDDKSAYITGADIPVNGGL
HLS
>Mature_243_residues
MNTKAVVVTGATGGIGWTIAEYLMSLEYFVIGTTHRAPQSACEQWIEMADKRGVLIKYDSNVDNLSIDTLIEVANGHSIY
GLVNNLGITRDSSFKKMTSEQWKQVMDVNLFSVFSLTQCIFNHMALSGSGRIVNISSVNAHRGQFGQVNYCAAKAGLLGF
TKALSLEGAKSGITVNSVSPGYTDTKMLSHIPTDILQSIKDSVPMKRLAKPIEIAHAVAFLLDDKSAYITGADIPVNGGL
HLS

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=162, Percent_Identity=38.2716049382716, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI40254992, Length=242, Percent_Identity=31.404958677686, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4504505, Length=209, Percent_Identity=31.5789473684211, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI32483357, Length=247, Percent_Identity=28.3400809716599, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI19923817, Length=256, Percent_Identity=29.296875, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4758504, Length=251, Percent_Identity=27.0916334661355, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI10190704, Length=261, Percent_Identity=26.4367816091954, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI7705925, Length=162, Percent_Identity=30.8641975308642, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI59889578, Length=168, Percent_Identity=30.952380952381, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI126723750, Length=251, Percent_Identity=25.0996015936255, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI66933014, Length=168, Percent_Identity=27.9761904761905, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI31542939, Length=263, Percent_Identity=25.8555133079848, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI83715985, Length=251, Percent_Identity=25.4980079681275, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787335, Length=242, Percent_Identity=41.7355371900826, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI87082100, Length=170, Percent_Identity=36.4705882352941, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789208, Length=242, Percent_Identity=29.7520661157025, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1788459, Length=247, Percent_Identity=30.7692307692308, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI2367175, Length=243, Percent_Identity=30.4526748971193, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786812, Length=249, Percent_Identity=30.9236947791165, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790717, Length=243, Percent_Identity=27.9835390946502, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87082160, Length=160, Percent_Identity=32.5, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789378, Length=148, Percent_Identity=31.7567567567568, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786701, Length=190, Percent_Identity=31.0526315789474, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787905, Length=242, Percent_Identity=24.7933884297521, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1789057, Length=260, Percent_Identity=26.5384615384615, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI2367365, Length=232, Percent_Identity=25.4310344827586, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=244, Percent_Identity=35.655737704918, Blast_Score=140, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25147288, Length=167, Percent_Identity=42.5149700598802, Blast_Score=133, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17560676, Length=248, Percent_Identity=29.4354838709677, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17561402, Length=250, Percent_Identity=31.2, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17568967, Length=205, Percent_Identity=32.1951219512195, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115534694, Length=241, Percent_Identity=32.3651452282158, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71994604, Length=245, Percent_Identity=29.3877551020408, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562910, Length=267, Percent_Identity=31.0861423220974, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17562908, Length=265, Percent_Identity=30.9433962264151, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=261, Percent_Identity=29.5019157088123, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=167, Percent_Identity=32.3353293413174, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=264, Percent_Identity=30.3030303030303, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17551412, Length=265, Percent_Identity=30.188679245283, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=270, Percent_Identity=28.5185185185185, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=29.9242424242424, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538480, Length=266, Percent_Identity=30.8270676691729, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=261, Percent_Identity=28.735632183908, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=266, Percent_Identity=29.6992481203008, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=158, Percent_Identity=30.379746835443, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=29.0076335877863, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=263, Percent_Identity=30.4182509505703, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=267, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=268, Percent_Identity=26.4925373134328, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=176, Percent_Identity=31.25, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=260, Percent_Identity=27.6923076923077, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17563726, Length=267, Percent_Identity=27.7153558052434, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=209, Percent_Identity=33.9712918660287, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639444, Length=244, Percent_Identity=36.0655737704918, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI23397609, Length=249, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21357041, Length=250, Percent_Identity=31.6, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18859837, Length=243, Percent_Identity=34.5679012345679, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=30.9236947791165, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24644339, Length=166, Percent_Identity=34.9397590361446, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI28571526, Length=250, Percent_Identity=29.6, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24644337, Length=172, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17737361, Length=150, Percent_Identity=28.6666666666667, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281364892, Length=222, Percent_Identity=28.3783783783784, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011283
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.36 [H]

Molecular weight: Translated: 26215; Mature: 26215

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKAVVVTGATGGIGWTIAEYLMSLEYFVIGTTHRAPQSACEQWIEMADKRGVLIKYDS
CCCCEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECC
NVDNLSIDTLIEVANGHSIYGLVNNLGITRDSSFKKMTSEQWKQVMDVNLFSVFSLTQCI
CCCCEEHHHHHHHCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FNHMALSGSGRIVNISSVNAHRGQFGQVNYCAAKAGLLGFTKALSLEGAKSGITVNSVSP
HHHHEECCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GYTDTKMLSHIPTDILQSIKDSVPMKRLAKPIEIAHAVAFLLDDKSAYITGADIPVNGGL
CCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCE
HLS
ECC
>Mature Secondary Structure
MNTKAVVVTGATGGIGWTIAEYLMSLEYFVIGTTHRAPQSACEQWIEMADKRGVLIKYDS
CCCCEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECC
NVDNLSIDTLIEVANGHSIYGLVNNLGITRDSSFKKMTSEQWKQVMDVNLFSVFSLTQCI
CCCCEEHHHHHHHCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FNHMALSGSGRIVNISSVNAHRGQFGQVNYCAAKAGLLGFTKALSLEGAKSGITVNSVSP
HHHHEECCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GYTDTKMLSHIPTDILQSIKDSVPMKRLAKPIEIAHAVAFLLDDKSAYITGADIPVNGGL
CCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCE
HLS
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7635832 [H]