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The map label for this gene is mak [H]

Identifier: 119775366

GI number: 119775366

Start: 2695484

End: 2696383

Strand: Reverse

Name: mak [H]

Synonym: Sama_2231

Alternate gene names: 119775366

Gene position: 2696383-2695484 (Counterclockwise)

Preceding gene: 119775367

Following gene: 119775365

Centisome position: 62.62

GC content: 57.44

Gene sequence:

>900_bases
ATGATGCGAATGGGGGTGGATCTCGGTGGCACCAAGATTGAGCTGGTGGCCTTGGGAGAAGATGGCAGTGAGCTTTTCCG
TAAGCGTATTGCGACGCCAAGAGAATATCAGGGCACTCTGAATGCCGTTGTGACCCTGGTGAACGAAGCCGAAGCCACGC
TGGGGACTCAGGGTAGTCTCGGTATTGGCATTCCCGGCGTGATTTCTCCCTATACAGGGCTGGTGAAAAATGCCAACTCG
ACCTGGATTAATGGTCATCCTCTTGACCGTGACCTGGGTGCTCTACTCAACCGGGAAGTGCGGGTCGCCAACGACGCCAA
CTGTTTTGCGGTCTCTGAGGCCGTAGATGGTGCGGCTGCCGGAAAACGCGTGGTGTTTGGCGCCATTTTAGGTACGGGTT
GCGGCGCCGGGCTTGCCTTCGATGGCCGGGTGCATGAAGGCGGCAACGGCATTGGCGGTGAGTGGGGCCATAACCCCTTG
CCCTGGATGCGCCCGGATGAATTCAACACCACCGAATGCTTTTGCGGCAACAAAGATTGCATCGAGACCTTTGTCTCTGG
CACTGGCTTTGTGCGTGATTTCAGGAACAGTGGTGGCACCGCTCAAAATGGCGCGGAAATCATGTCTTTGGTAGATGCTG
GTGATGAGCTCGCCAATCTGGTATTTGACCGCTATCTGGACCGGTTGGCCCGCTCGCTTGCCCATGTGATCAATATGCTC
GACCCCGATGCCATCGTCCTCGGCGGTGGTATGTCCAATGTGCAGGCTATCTATGCCCGGCTGCCTGCCATTTTGCCAAA
GTATGTTGTGGGCCGTGAGTGCCGCACCCCTGTGGTACAGAACCTCTATGGCTGTTCTTCCGGCGTCCGCGGCGCCGCCT
GGCTGTGGGAAAAGCGCTGA

Upstream 100 bases:

>100_bases
GGTTTACAACAAAAAAGAACAGCTTCTTAACCCTTGGTTTATGGCAACTGCCCCGGGGTGGAACACTCATTAACAGCCCA
GGCTGAAATGGAGAAAGCAG

Downstream 100 bases:

>100_bases
TCCTGCTATCGTCGCGATTTTCCGATGACTCAGAAAACGCCTTGTCTGCTTTCATGCCGGCAAGGCGTTTTTTCAGCTGG
CCACAACTTCTCTCCTTGGG

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MMRMGVDLGGTKIELVALGEDGSELFRKRIATPREYQGTLNAVVTLVNEAEATLGTQGSLGIGIPGVISPYTGLVKNANS
TWINGHPLDRDLGALLNREVRVANDANCFAVSEAVDGAAAGKRVVFGAILGTGCGAGLAFDGRVHEGGNGIGGEWGHNPL
PWMRPDEFNTTECFCGNKDCIETFVSGTGFVRDFRNSGGTAQNGAEIMSLVDAGDELANLVFDRYLDRLARSLAHVINML
DPDAIVLGGGMSNVQAIYARLPAILPKYVVGRECRTPVVQNLYGCSSGVRGAAWLWEKR

Sequences:

>Translated_299_residues
MMRMGVDLGGTKIELVALGEDGSELFRKRIATPREYQGTLNAVVTLVNEAEATLGTQGSLGIGIPGVISPYTGLVKNANS
TWINGHPLDRDLGALLNREVRVANDANCFAVSEAVDGAAAGKRVVFGAILGTGCGAGLAFDGRVHEGGNGIGGEWGHNPL
PWMRPDEFNTTECFCGNKDCIETFVSGTGFVRDFRNSGGTAQNGAEIMSLVDAGDELANLVFDRYLDRLARSLAHVINML
DPDAIVLGGGMSNVQAIYARLPAILPKYVVGRECRTPVVQNLYGCSSGVRGAAWLWEKR
>Mature_299_residues
MMRMGVDLGGTKIELVALGEDGSELFRKRIATPREYQGTLNAVVTLVNEAEATLGTQGSLGIGIPGVISPYTGLVKNANS
TWINGHPLDRDLGALLNREVRVANDANCFAVSEAVDGAAAGKRVVFGAILGTGCGAGLAFDGRVHEGGNGIGGEWGHNPL
PWMRPDEFNTTECFCGNKDCIETFVSGTGFVRDFRNSGGTAQNGAEIMSLVDAGDELANLVFDRYLDRLARSLAHVINML
DPDAIVLGGGMSNVQAIYARLPAILPKYVVGRECRTPVVQNLYGCSSGVRGAAWLWEKR

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=301, Percent_Identity=60.1328903654485, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787363, Length=300, Percent_Identity=41.6666666666667, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1790522, Length=304, Percent_Identity=27.3026315789474, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786891, Length=275, Percent_Identity=23.6363636363636, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787878, Length=206, Percent_Identity=26.2135922330097, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31758; Mature: 31758

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRMGVDLGGTKIELVALGEDGSELFRKRIATPREYQGTLNAVVTLVNEAEATLGTQGSL
CCEECCCCCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GIGIPGVISPYTGLVKNANSTWINGHPLDRDLGALLNREVRVANDANCFAVSEAVDGAAA
CCCCCCHHCCHHHHHHCCCCCEECCCCCCHHHHHHHCCCEEECCCCCEEEEHHHHCCHHC
GKRVVFGAILGTGCGAGLAFDGRVHEGGNGIGGEWGHNPLPWMRPDEFNTTECFCGNKDC
CCHHHHHHHHHCCCCCCCEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHH
IETFVSGTGFVRDFRNSGGTAQNGAEIMSLVDAGDELANLVFDRYLDRLARSLAHVINML
HHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DPDAIVLGGGMSNVQAIYARLPAILPKYVVGRECRTPVVQNLYGCSSGVRGAAWLWEKR
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHCCCCC
>Mature Secondary Structure
MMRMGVDLGGTKIELVALGEDGSELFRKRIATPREYQGTLNAVVTLVNEAEATLGTQGSL
CCEECCCCCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GIGIPGVISPYTGLVKNANSTWINGHPLDRDLGALLNREVRVANDANCFAVSEAVDGAAA
CCCCCCHHCCHHHHHHCCCCCEECCCCCCHHHHHHHCCCEEECCCCCEEEEHHHHCCHHC
GKRVVFGAILGTGCGAGLAFDGRVHEGGNGIGGEWGHNPLPWMRPDEFNTTECFCGNKDC
CCHHHHHHHHHCCCCCCCEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHH
IETFVSGTGFVRDFRNSGGTAQNGAEIMSLVDAGDELANLVFDRYLDRLARSLAHVINML
HHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DPDAIVLGGGMSNVQAIYARLPAILPKYVVGRECRTPVVQNLYGCSSGVRGAAWLWEKR
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]