Click here to switch to the map view.

The map label for this gene is queA

Identifier: 119775329

GI number: 119775329

Start: 2652619

End: 2653656

Strand: Reverse

Name: queA

Synonym: Sama_2194

Alternate gene names: 119775329

Gene position: 2653656-2652619 (Counterclockwise)

Preceding gene: 119775333

Following gene: 119775328

Centisome position: 61.62

GC content: 55.01

Gene sequence:

>1038_bases
ATGCGAGTAGCTGACTTTACCTTTGATCTTCCCGATGAACTGATTGCCCGCTATCCCATGGCAGAGCGCACCGCGTCGCG
TCTGTTGCATCTGGACGGCAGCACTGGTGCCTTGGCCGATCGTCAGTTCACCGATATTCTGGCGCTGATTGAGCCGGGCG
ATCTGATGATTTTCAATAACACCCGGGTGATACCGGCTCGTCTCTTTGGCCAAAAGGCAAGCGGCGGCAAGTTGGAGATC
CTGGTGGAGCGTATGCTTGATGACAAGCGCATTTTGGCCCATGTTCGCTCGTCCAAATCCCCCAAACCCGGCGCCGAGAT
TATCCTGGACGGCGGCTTTAAGATGACGATGGATGCCCGTCATGATGCACTGTTTGAGCTGTCGCTCAAGGATGATAAGA
CCATCCTCGAAGTGCTTGAAGCCGTAGGCCATATGCCGCTGCCGCCTTATATCGATCGCCCCGATGAAGATACCGACAAA
GAGCGTTATCAAACCGTCTATAACGAGCGCCCCGGCGCCGTTGCGGCTCCTACTGCCGGTCTTCACTTCGATGAAAGCAT
ACTCGCCGCCCTCAAAGCCAAAGGTGTGGACATGGCATTTGTGACCCTGCACGTGGGAGCGGGTACCTTTCAGCCGGTGC
GGGTAGACAACGTGCTCGAGCACAAAATGCATTCAGAATGGGCCGAAGTACCTGCCGATGTGGTTGAAAAAATCCGCGCC
ACCAAGGCCGCAGGTAAGCGGGTGATTGCCGTGGGGACCACCTCAGTGCGCTCCCTCGAGAGTGCCGCCAAGGCAAGTGA
GGGTGAACTCGAGCCATTTTGCGGTGATACCGATATCTTTATCTTCCCCGGTTTCGAGTTCAAAGTAGTCGATGCCATGG
TGACCAACTTCCACCTGCCGGAGTCGACACTCATCATGCTGGTGAGCGCCTTTGCCGGCTTCGATGAGGTAATTGGCGCC
TATCGCCATGCAGTTGAACAAAAATACCGCTTTTTCAGTTATGGCGATGCCATGTTTGTGACTAAAAAAGCCCCCTAA

Upstream 100 bases:

>100_bases
GTACTCAAAGAGCTATGCAAAGGTCCAAGTCGTCTTGATCTCGGCCGCCTGTGCCCCTAGACTACGCGCGTCTTTTTTGG
CCATGGCGTGAGAGTTATAG

Downstream 100 bases:

>100_bases
ACGCTTTTTTTGATTTAAAATGCCTGCCCAACCATCTGGTTGGGCTTTTTTTTGGTGGCAGCGCCTTGAAGGGGCTAGCT
GCCACCCCCATGTGAGTTGA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRVADFTFDLPDELIARYPMAERTASRLLHLDGSTGALADRQFTDILALIEPGDLMIFNNTRVIPARLFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKPGAEIILDGGFKMTMDARHDALFELSLKDDKTILEVLEAVGHMPLPPYIDRPDEDTDK
ERYQTVYNERPGAVAAPTAGLHFDESILAALKAKGVDMAFVTLHVGAGTFQPVRVDNVLEHKMHSEWAEVPADVVEKIRA
TKAAGKRVIAVGTTSVRSLESAAKASEGELEPFCGDTDIFIFPGFEFKVVDAMVTNFHLPESTLIMLVSAFAGFDEVIGA
YRHAVEQKYRFFSYGDAMFVTKKAP

Sequences:

>Translated_345_residues
MRVADFTFDLPDELIARYPMAERTASRLLHLDGSTGALADRQFTDILALIEPGDLMIFNNTRVIPARLFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKPGAEIILDGGFKMTMDARHDALFELSLKDDKTILEVLEAVGHMPLPPYIDRPDEDTDK
ERYQTVYNERPGAVAAPTAGLHFDESILAALKAKGVDMAFVTLHVGAGTFQPVRVDNVLEHKMHSEWAEVPADVVEKIRA
TKAAGKRVIAVGTTSVRSLESAAKASEGELEPFCGDTDIFIFPGFEFKVVDAMVTNFHLPESTLIMLVSAFAGFDEVIGA
YRHAVEQKYRFFSYGDAMFVTKKAP
>Mature_345_residues
MRVADFTFDLPDELIARYPMAERTASRLLHLDGSTGALADRQFTDILALIEPGDLMIFNNTRVIPARLFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKPGAEIILDGGFKMTMDARHDALFELSLKDDKTILEVLEAVGHMPLPPYIDRPDEDTDK
ERYQTVYNERPGAVAAPTAGLHFDESILAALKAKGVDMAFVTLHVGAGTFQPVRVDNVLEHKMHSEWAEVPADVVEKIRA
TKAAGKRVIAVGTTSVRSLESAAKASEGELEPFCGDTDIFIFPGFEFKVVDAMVTNFHLPESTLIMLVSAFAGFDEVIGA
YRHAVEQKYRFFSYGDAMFVTKKAP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=70.6395348837209, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_SHEAM (A1S7P3)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928069.1
- ProteinModelPortal:   A1S7P3
- SMR:   A1S7P3
- STRING:   A1S7P3
- GeneID:   4604444
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2194
- NMPDR:   fig|326297.7.peg.2062
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   A1S7P3
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38088; Mature: 38088

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFTFDLPDELIARYPMAERTASRLLHLDGSTGALADRQFTDILALIEPGDLMIFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHEEEECCCCCCCCCHHHHHHHEEECCCCEEEECC
TRVIPARLFGQKASGGKLEILVERMLDDKRILAHVRSSKSPKPGAEIILDGGFKMTMDAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEECCEEEEEECC
HDALFELSLKDDKTILEVLEAVGHMPLPPYIDRPDEDTDKERYQTVYNERPGAVAAPTAG
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEECCCCC
LHFDESILAALKAKGVDMAFVTLHVGAGTFQPVRVDNVLEHKMHSEWAEVPADVVEKIRA
CCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHH
TKAAGKRVIAVGTTSVRSLESAAKASEGELEPFCGDTDIFIFPGFEFKVVDAMVTNFHLP
HHHCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHCCCC
ESTLIMLVSAFAGFDEVIGAYRHAVEQKYRFFSYGDAMFVTKKAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCC
>Mature Secondary Structure
MRVADFTFDLPDELIARYPMAERTASRLLHLDGSTGALADRQFTDILALIEPGDLMIFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHEEEECCCCCCCCCHHHHHHHEEECCCCEEEECC
TRVIPARLFGQKASGGKLEILVERMLDDKRILAHVRSSKSPKPGAEIILDGGFKMTMDAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEECCEEEEEECC
HDALFELSLKDDKTILEVLEAVGHMPLPPYIDRPDEDTDKERYQTVYNERPGAVAAPTAG
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEECCCCC
LHFDESILAALKAKGVDMAFVTLHVGAGTFQPVRVDNVLEHKMHSEWAEVPADVVEKIRA
CCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHH
TKAAGKRVIAVGTTSVRSLESAAKASEGELEPFCGDTDIFIFPGFEFKVVDAMVTNFHLP
HHHCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHCCCC
ESTLIMLVSAFAGFDEVIGAYRHAVEQKYRFFSYGDAMFVTKKAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA