Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 119775328

GI number: 119775328

Start: 2651342

End: 2652466

Strand: Reverse

Name: tgt [H]

Synonym: Sama_2193

Alternate gene names: 119775328

Gene position: 2652466-2651342 (Counterclockwise)

Preceding gene: 119775329

Following gene: 119775327

Centisome position: 61.6

GC content: 53.16

Gene sequence:

>1125_bases
ATGAAATTTGAATTGATTACTACCCAGGGTCGCGCCCGTCGCGGCAGACTGGTTTTCGAGCGTGGCACGGTAGAAACCCC
GGCCTTTATGCCTGTGGGTACCTACGGCACAGTCAAGGGCATGACCCCGGAAGAAGTGCGTGCTACCGGCGCCGATATCC
TGCTTGGCAACACCTTCCATCTGTGGTTGCGCCCCGGTGAAGAAATCATGCGCAAGCACGGCGATCTGCACGACTTTATG
AACTGGCAGCGTCCTATTCTCACCGACTCGGGCGGTTTCCAGGTATTCAGCCTTGGCGATATCCGTAAGATCACCGAGGA
AGGTGTGCATTTCCGCTCGCCCATCAACGGCGAAAAGATTTTTATGGATGCCGAAAAGTCGATGCAAATCCAGCACTCGC
TCGGCAGTGACGTAGTGATGATTTTCGATGAGTGCACGCCCTATCCGGCGACGCACGACGAAGCGCGCAAGTCCATGCAG
ATGTCGCTGCGCTGGGCCAAGCGCTCTCGCGATGAGTTTGATCGCCTCGAAAACCCCAACTCTCTTTTTGGGATTATTCA
GGGCAGCGTGTACGAAGATTTGCGTGACGAGAGCCTAAAAGGGCTTCTCGAAATCGGTTTTGACGGTTATGCCGTCGGTG
GTCTGGCGGTGGGTGAGCCCAAGGAAGACATGCACAGAGTGCTTGAGCATGTGTGTCCGCAAATCCCGGCCGACAAACCA
CGTTATCTGATGGGGGTAGGTAAACCGGAAGATTTGGTTGAAGGCGTGCGTCGTGGTGTCGACATGTTCGACTGTGTAAT
GCCCACCCGTAATGCCCGCAACGGTCATCTGTTTACCAGCGAAGGTGTTATCAAAATTCGCAATGCGCGTCATCGTGATG
ACACATCGCCACTCGATCCTAAATGTGATTGCTATACCTGCACCAATTATTCCCGTGCGTATTTGCATCATTTGGATCGT
TGCAACGAAATCCTCGGAGCCAGGCTCAATACCATTCACAACCTGCGTTATTACCAAAGGTTGATGGAGGGTTTGCGCGG
CGCCATTGAGACGGGTACATTAGACGCCTTTGTTGCGGACTTCTATACCAGTCAAGGGCGTGAAGTTCCTGAGTTAAAAG
ACTAA

Upstream 100 bases:

>100_bases
TGGTGGCAGCGCCTTGAAGGGGCTAGCTGCCACCCCCATGTGAGTTGAATCGGCCGTTTGGCCCTCAATGCAGTCAGACT
GTTTCTCTGACGAGGTCATT

Downstream 100 bases:

>100_bases
CAATCAAGCAATAAGAAGAGAAAGCTATGTTTATTTCTAACGCGTATGCCAATGCAGCCGGTGCAGCACCCCAGGGTGGC
GGTACCATGGAACTTGTGAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKFELITTQGRARRGRLVFERGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFHLWLRPGEEIMRKHGDLHDFM
NWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKIFMDAEKSMQIQHSLGSDVVMIFDECTPYPATHDEARKSMQ
MSLRWAKRSRDEFDRLENPNSLFGIIQGSVYEDLRDESLKGLLEIGFDGYAVGGLAVGEPKEDMHRVLEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDPKCDCYTCTNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVADFYTSQGREVPELKD

Sequences:

>Translated_374_residues
MKFELITTQGRARRGRLVFERGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFHLWLRPGEEIMRKHGDLHDFM
NWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKIFMDAEKSMQIQHSLGSDVVMIFDECTPYPATHDEARKSMQ
MSLRWAKRSRDEFDRLENPNSLFGIIQGSVYEDLRDESLKGLLEIGFDGYAVGGLAVGEPKEDMHRVLEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDPKCDCYTCTNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVADFYTSQGREVPELKD
>Mature_374_residues
MKFELITTQGRARRGRLVFERGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFHLWLRPGEEIMRKHGDLHDFM
NWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKIFMDAEKSMQIQHSLGSDVVMIFDECTPYPATHDEARKSMQ
MSLRWAKRSRDEFDRLENPNSLFGIIQGSVYEDLRDESLKGLLEIGFDGYAVGGLAVGEPKEDMHRVLEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDPKCDCYTCTNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVADFYTSQGREVPELKD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=361, Percent_Identity=43.7673130193906, Blast_Score=305, Evalue=5e-83,
Organism=Escherichia coli, GI1786607, Length=373, Percent_Identity=82.573726541555, Blast_Score=659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=379, Percent_Identity=40.8970976253298, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=155, Percent_Identity=31.6129032258064, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=42.6592797783933, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI21355311, Length=158, Percent_Identity=31.0126582278481, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42520; Mature: 42520

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFELITTQGRARRGRLVFERGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFH
CEEEEEECCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGEEIMRKHGDLHDFMNWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKI
EEECCCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCEE
FMDAEKSMQIQHSLGSDVVMIFDECTPYPATHDEARKSMQMSLRWAKRSRDEFDRLENPN
EEECHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
SLFGIIQGSVYEDLRDESLKGLLEIGFDGYAVGGLAVGEPKEDMHRVLEHVCPQIPADKP
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDP
CEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCCC
KCDCYTCTNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVAD
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FYTSQGREVPELKD
HHHHCCCCCCCCCC
>Mature Secondary Structure
MKFELITTQGRARRGRLVFERGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFH
CEEEEEECCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGEEIMRKHGDLHDFMNWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKI
EEECCCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCEE
FMDAEKSMQIQHSLGSDVVMIFDECTPYPATHDEARKSMQMSLRWAKRSRDEFDRLENPN
EEECHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
SLFGIIQGSVYEDLRDESLKGLLEIGFDGYAVGGLAVGEPKEDMHRVLEHVCPQIPADKP
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDP
CEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCCC
KCDCYTCTNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVAD
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FYTSQGREVPELKD
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA