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The map label for this gene is trpCF [H]

Identifier: 119775267

GI number: 119775267

Start: 2577322

End: 2578710

Strand: Direct

Name: trpCF [H]

Synonym: Sama_2132

Alternate gene names: 119775267

Gene position: 2577322-2578710 (Clockwise)

Preceding gene: 119775266

Following gene: 119775268

Centisome position: 59.85

GC content: 56.23

Gene sequence:

>1389_bases
ATGAGCAATGTGTTAACCAAGATTATCGACACCAAATACGCCCATATCGATGCCCTCAAGCAGCGATTCCCGGAAGCCAG
CCTGACGCCGAAAATCTCTGACCGCAGCCTCTATCAGGCGCTGGCAGCACCCAACGCAGGTTTTATTCTGGAATGTAAAA
AGGCCAGCCCATCCAAGGGGCTGATCCGTGCCGACTTTGACCCTGTGGCCATCGTCAGCATTTACAGCCGTTATGCTGCC
GGCATATCAGTGCTGACCGATGAGCAGTTTTTTCAGGGCGACATGGACTATATCCCACAGGTGCGTGCCGCTGTCTCCCA
GCCAGTGCTGTGCAAAGACTTTTTTATCTCGCCCTATCAGGTCAGGCTCGCGGCCCATCAGGGCGCCGATGCCATACTCT
TGATGCTGAGCGTGCTGGATGACGATGATTACCGACGCCTTGCCGACGAAGCCGCCAAATATCAGCTCGACATTCTCACC
GAAGTGAGCAACGAAGACGAGCTTCGCCGCGCCATCGCTCTCAGAGCTCCCATTATTGGCATCAACAACCGTAACTTGCG
TGACCTGAGCACAGACCTCGCCACCACAGAAGCCCTGGCACCTCACATCCCCGCCGATACCCTGGTCATCAGCGAATCCG
GAATTTACACCCACGAACAGGTAAAGCGCCTCGCCCCTCTGGTGCAGGGATTTTTGGTGGGCAGCTCCCTGATGGCGGAA
GCGAACCTGGATTTAGCCTGTCGACGCCTGATTTATGGCGAAAACAAGGTCTGCGGCCTGACCCGAGAGGCGGATATCAC
GGCCGCCGCCAACGCTGGTGCCGTCTATGGCGGACTGATTTTTGCCGAAAAATCCCCTCGCTGCGTATCAGAAGATAGAG
CACGGGCCCTGAGCGCATTCAATCGCGCCGCAAGCCAGCCGCTGGCGCTCGTAGGCGTATTTGTGGATGAAGCTGCGGCC
AAGGTAGTCAGTCTTGCCAAGACGTTGGGACTGGCGGCAGTGCAACTGCACGGCAAGGAAGATGAAGCTTATATCGATGA
CCTGCGAAGCCAGTTGTCACAGGAGTTACCCGAAGGCTGTGAGATTTGGAAAGCCGTGGCCGTTGAAGCAGGCAAGGCAT
CTGCCATCCCCCATGGGGCAGACCGCATTGTATATGACAGCAAAAACAGTGCTGGCTTTGGCGGGACAGGTGTGTGTTTT
GATTGGCAACTGAATCTTGCAGACAAACACAGGGCCATGCTGGCCGGTGGTCTCAATGCAAACAATGCCGCCACCGCCGC
CAATCAGGGCTTTTTGGGGCTGGACTTTAATTCAGGATTGGAGGAAGCGCCCGGCATCAAGTCTGCCGCGCTTATCGAGC
AGGCATTTGCCAGCCTTCGCAACTACTGA

Upstream 100 bases:

>100_bases
AAGCTTGCCATTGACACCCTCGCCTCCGGCAAGGCATATGAAAAACTGATTGGCCTCGCCGCCATGAGTCAGCAGGACAA
GGCGTAAGGAATTAAGCCCC

Downstream 100 bases:

>100_bases
CAAGCACTTAACCGAACAGAATTATTGGTGGTAAAGCCGCCATAGCAGATTGACTGACTTTGGAGAGATACAATGAGCGA
GCTGAAACTCGATCCCTACT

Product: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSNVLTKIIDTKYAHIDALKQRFPEASLTPKISDRSLYQALAAPNAGFILECKKASPSKGLIRADFDPVAIVSIYSRYAA
GISVLTDEQFFQGDMDYIPQVRAAVSQPVLCKDFFISPYQVRLAAHQGADAILLMLSVLDDDDYRRLADEAAKYQLDILT
EVSNEDELRRAIALRAPIIGINNRNLRDLSTDLATTEALAPHIPADTLVISESGIYTHEQVKRLAPLVQGFLVGSSLMAE
ANLDLACRRLIYGENKVCGLTREADITAAANAGAVYGGLIFAEKSPRCVSEDRARALSAFNRAASQPLALVGVFVDEAAA
KVVSLAKTLGLAAVQLHGKEDEAYIDDLRSQLSQELPEGCEIWKAVAVEAGKASAIPHGADRIVYDSKNSAGFGGTGVCF
DWQLNLADKHRAMLAGGLNANNAATAANQGFLGLDFNSGLEEAPGIKSAALIEQAFASLRNY

Sequences:

>Translated_462_residues
MSNVLTKIIDTKYAHIDALKQRFPEASLTPKISDRSLYQALAAPNAGFILECKKASPSKGLIRADFDPVAIVSIYSRYAA
GISVLTDEQFFQGDMDYIPQVRAAVSQPVLCKDFFISPYQVRLAAHQGADAILLMLSVLDDDDYRRLADEAAKYQLDILT
EVSNEDELRRAIALRAPIIGINNRNLRDLSTDLATTEALAPHIPADTLVISESGIYTHEQVKRLAPLVQGFLVGSSLMAE
ANLDLACRRLIYGENKVCGLTREADITAAANAGAVYGGLIFAEKSPRCVSEDRARALSAFNRAASQPLALVGVFVDEAAA
KVVSLAKTLGLAAVQLHGKEDEAYIDDLRSQLSQELPEGCEIWKAVAVEAGKASAIPHGADRIVYDSKNSAGFGGTGVCF
DWQLNLADKHRAMLAGGLNANNAATAANQGFLGLDFNSGLEEAPGIKSAALIEQAFASLRNY
>Mature_461_residues
SNVLTKIIDTKYAHIDALKQRFPEASLTPKISDRSLYQALAAPNAGFILECKKASPSKGLIRADFDPVAIVSIYSRYAAG
ISVLTDEQFFQGDMDYIPQVRAAVSQPVLCKDFFISPYQVRLAAHQGADAILLMLSVLDDDDYRRLADEAAKYQLDILTE
VSNEDELRRAIALRAPIIGINNRNLRDLSTDLATTEALAPHIPADTLVISESGIYTHEQVKRLAPLVQGFLVGSSLMAEA
NLDLACRRLIYGENKVCGLTREADITAAANAGAVYGGLIFAEKSPRCVSEDRARALSAFNRAASQPLALVGVFVDEAAAK
VVSLAKTLGLAAVQLHGKEDEAYIDDLRSQLSQELPEGCEIWKAVAVEAGKASAIPHGADRIVYDSKNSAGFGGTGVCFD
WQLNLADKHRAMLAGGLNANNAATAANQGFLGLDFNSGLEEAPGIKSAALIEQAFASLRNY

Specific function: Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain [H]

COG id: COG0134

COG function: function code E; Indole-3-glycerol phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=466, Percent_Identity=51.7167381974249, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322638, Length=235, Percent_Identity=32.7659574468085, Blast_Score=109, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013798
- InterPro:   IPR001468
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00218 IGPS; PF00697 PRAI [H]

EC number: =4.1.1.48; =5.3.1.24 [H]

Molecular weight: Translated: 49646; Mature: 49515

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00614 IGPS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVLTKIIDTKYAHIDALKQRFPEASLTPKISDRSLYQALAAPNAGFILECKKASPSKG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
LIRADFDPVAIVSIYSRYAAGISVLTDEQFFQGDMDYIPQVRAAVSQPVLCKDFFISPYQ
EEEECCCCHHHHHHHHHHHCCCEEEECHHHHCCCHHHHHHHHHHHCCCCEEHHHCCCCEE
VRLAAHQGADAILLMLSVLDDDDYRRLADEAAKYQLDILTEVSNEDELRRAIALRAPIIG
EEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEE
INNRNLRDLSTDLATTEALAPHIPADTLVISESGIYTHEQVKRLAPLVQGFLVGSSLMAE
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ANLDLACRRLIYGENKVCGLTREADITAAANAGAVYGGLIFAEKSPRCVSEDRARALSAF
CCHHHHHHHHHCCCCCEECCCCCCCCEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHH
NRAASQPLALVGVFVDEAAAKVVSLAKTLGLAAVQLHGKEDEAYIDDLRSQLSQELPEGC
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHCCHHH
EIWKAVAVEAGKASAIPHGADRIVYDSKNSAGFGGTGVCFDWQLNLADKHRAMLAGGLNA
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCC
NNAATAANQGFLGLDFNSGLEEAPGIKSAALIEQAFASLRNY
CCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNVLTKIIDTKYAHIDALKQRFPEASLTPKISDRSLYQALAAPNAGFILECKKASPSKG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
LIRADFDPVAIVSIYSRYAAGISVLTDEQFFQGDMDYIPQVRAAVSQPVLCKDFFISPYQ
EEEECCCCHHHHHHHHHHHCCCEEEECHHHHCCCHHHHHHHHHHHCCCCEEHHHCCCCEE
VRLAAHQGADAILLMLSVLDDDDYRRLADEAAKYQLDILTEVSNEDELRRAIALRAPIIG
EEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEE
INNRNLRDLSTDLATTEALAPHIPADTLVISESGIYTHEQVKRLAPLVQGFLVGSSLMAE
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ANLDLACRRLIYGENKVCGLTREADITAAANAGAVYGGLIFAEKSPRCVSEDRARALSAF
CCHHHHHHHHHCCCCCEECCCCCCCCEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHH
NRAASQPLALVGVFVDEAAAKVVSLAKTLGLAAVQLHGKEDEAYIDDLRSQLSQELPEGC
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHCCHHH
EIWKAVAVEAGKASAIPHGADRIVYDSKNSAGFGGTGVCFDWQLNLADKHRAMLAGGLNA
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCC
NNAATAANQGFLGLDFNSGLEEAPGIKSAALIEQAFASLRNY
CCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]