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The map label for this gene is tolB

Identifier: 119775221

GI number: 119775221

Start: 2529649

End: 2530974

Strand: Reverse

Name: tolB

Synonym: Sama_2086

Alternate gene names: 119775221

Gene position: 2530974-2529649 (Counterclockwise)

Preceding gene: 119775222

Following gene: 119775220

Centisome position: 58.78

GC content: 55.35

Gene sequence:

>1326_bases
ATGAAGATCATCGGAAAGTGGGCGCTGCTGATAATTGCCGCTCTGTCTTTGCCCGTGAAGGCCGCTTTGGACATCGTTAT
CACCGAAGGTGTTGATGCTGCCCGACCCATTGCAGTGGTGCCCTTTGTTTGGCAGGGAACTGGCCCGGTTCCATCACAGA
TTTCAGATGTTGTGGCATCGGATCTCACCCGCAGTGGTACTTTTAAACCTTTGGATGCGTTGGGATTGCCGCAAAGCGGT
ATTTCTACCCTCGCTGAATTTGCACCGCAAAAATGGACCGGTATTAACGCCGAAGCGGCCGTGGTTGGCGCCATCAAGCC
TTATGGCCCGGATCAGTATCTGGTGACCTTTGATTTGATTGATTTGCTGAAAGCGCAATTGGCCGGTCAGGCGCCGCTGA
ACAAGCAGGACCTGCTGTTGGATAGCCGCCAGACGGTGATTACCGCGGCCCAGTTCCGCCAGTATGGTCATCGCATCAGC
GATGTGGTCTACGAAAAACTCACCGGTATTCGCGGCGCTTTTTTGACCCGTATCGCTTACGTGGTGGTAAATCATGCCGA
GAAAGCCCCATACCGTTTGATGATTGCCGATTATGACGGCTACAACGAGCACATGTTGCTGCGCTCCCCTGAGCCGCTGA
TGTCGCCTGCCTGGTCGCCGGATGGTCGCCGCCTGGCTTATGTCAGCTTTGAAAACCGCAAGGCCGAAATCTTTGTACAG
GACATCTTTACTCAGGCCCGTACCAAGGTCTCCAGCTTTCCCGGAATTAATGGTGCGCCAAGTTTTTCGCCCGATGGCAA
AACCTTGGCGGTGACGCTGTCTAAAGACGGTCAGAGCGAAATCTATACCATCGACATCGCCACCAAGACCACCCGCCGGA
TAACCAACCACTATGCTATCGACACAGAGCCAAGCTGGTTCCCCGATGGCAAGTCGCTGCTTATCACCTCTGAGCGTGGC
GGCAGACCTCAGCTGTATAAGGTGGAGCTGGACTCCGGCAAGTTGACCCGTCTGACCTTTGATGGTGAGTGGAACCTCGG
TGGCTCCATTACCCCGGACGGCCGCACCCTGATTTTTGTAAACCGTACCAACGGCAAGTTCAATATCGCTCGCATGGATC
TGGAAACCCGGTTTATGCAGGTGCTGACCACGACCCGTTTGGACGAATCTCCCAGCGTGGCGCCCAATGGCACCATGGTC
ATTTATGGCACCACGCATCAGGGCCGGCAGGTGCTCGCTGCCGTGTCTACGGATGGACGCTTTAAGGCAAGATTGCCCGC
GGGGCAGGGGGAGGTGAAATCACCTTCCTGGTCACCGTTCCTGTAA

Upstream 100 bases:

>100_bases
TACCAAGGCCGGACGTCTGCCGGTATCACCTGAGCCTGAGGTGTACCAGCTGATGAAAGAAATCAATTTGACCGTGCAAC
CGGAAATCTAAAGGAGTTTC

Downstream 100 bases:

>100_bases
CGAATTATCATCATCGATTGATTAAAGGATTTAAAAATGGAATTGAACAAACTGCTTAAGACCATGCTGATTGTGGCTCC
TATCATGGCTTTGTCTGCCT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKIIGKWALLIIAALSLPVKAALDIVITEGVDAARPIAVVPFVWQGTGPVPSQISDVVASDLTRSGTFKPLDALGLPQSG
ISTLAEFAPQKWTGINAEAAVVGAIKPYGPDQYLVTFDLIDLLKAQLAGQAPLNKQDLLLDSRQTVITAAQFRQYGHRIS
DVVYEKLTGIRGAFLTRIAYVVVNHAEKAPYRLMIADYDGYNEHMLLRSPEPLMSPAWSPDGRRLAYVSFENRKAEIFVQ
DIFTQARTKVSSFPGINGAPSFSPDGKTLAVTLSKDGQSEIYTIDIATKTTRRITNHYAIDTEPSWFPDGKSLLITSERG
GRPQLYKVELDSGKLTRLTFDGEWNLGGSITPDGRTLIFVNRTNGKFNIARMDLETRFMQVLTTTRLDESPSVAPNGTMV
IYGTTHQGRQVLAAVSTDGRFKARLPAGQGEVKSPSWSPFL

Sequences:

>Translated_441_residues
MKIIGKWALLIIAALSLPVKAALDIVITEGVDAARPIAVVPFVWQGTGPVPSQISDVVASDLTRSGTFKPLDALGLPQSG
ISTLAEFAPQKWTGINAEAAVVGAIKPYGPDQYLVTFDLIDLLKAQLAGQAPLNKQDLLLDSRQTVITAAQFRQYGHRIS
DVVYEKLTGIRGAFLTRIAYVVVNHAEKAPYRLMIADYDGYNEHMLLRSPEPLMSPAWSPDGRRLAYVSFENRKAEIFVQ
DIFTQARTKVSSFPGINGAPSFSPDGKTLAVTLSKDGQSEIYTIDIATKTTRRITNHYAIDTEPSWFPDGKSLLITSERG
GRPQLYKVELDSGKLTRLTFDGEWNLGGSITPDGRTLIFVNRTNGKFNIARMDLETRFMQVLTTTRLDESPSVAPNGTMV
IYGTTHQGRQVLAAVSTDGRFKARLPAGQGEVKSPSWSPFL
>Mature_441_residues
MKIIGKWALLIIAALSLPVKAALDIVITEGVDAARPIAVVPFVWQGTGPVPSQISDVVASDLTRSGTFKPLDALGLPQSG
ISTLAEFAPQKWTGINAEAAVVGAIKPYGPDQYLVTFDLIDLLKAQLAGQAPLNKQDLLLDSRQTVITAAQFRQYGHRIS
DVVYEKLTGIRGAFLTRIAYVVVNHAEKAPYRLMIADYDGYNEHMLLRSPEPLMSPAWSPDGRRLAYVSFENRKAEIFVQ
DIFTQARTKVSSFPGINGAPSFSPDGKTLAVTLSKDGQSEIYTIDIATKTTRRITNHYAIDTEPSWFPDGKSLLITSERG
GRPQLYKVELDSGKLTRLTFDGEWNLGGSITPDGRTLIFVNRTNGKFNIARMDLETRFMQVLTTTRLDESPSVAPNGTMV
IYGTTHQGRQVLAAVSTDGRFKARLPAGQGEVKSPSWSPFL

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=423, Percent_Identity=46.8085106382979, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_SHEAM (A1S7D5)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927961.1
- ProteinModelPortal:   A1S7D5
- SMR:   A1S7D5
- STRING:   A1S7D5
- GeneID:   4604336
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2086
- NMPDR:   fig|326297.7.peg.1959
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   A1S7D5
- ProtClustDB:   PRK04792
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- Gene3D:   G3DSA:3.40.50.10070
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48272; Mature: 48272

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIGKWALLIIAALSLPVKAALDIVITEGVDAARPIAVVPFVWQGTGPVPSQISDVVAS
CCCHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
DLTRSGTFKPLDALGLPQSGISTLAEFAPQKWTGINAEAAVVGAIKPYGPDQYLVTFDLI
HHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEEHHHH
DLLKAQLAGQAPLNKQDLLLDSRQTVITAAQFRQYGHRISDVVYEKLTGIRGAFLTRIAY
HHHHHHHCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VVVNHAEKAPYRLMIADYDGYNEHMLLRSPEPLMSPAWSPDGRRLAYVSFENRKAEIFVQ
HHHCCCCCCCEEEEEEECCCCCCEEEEECCCHHCCCCCCCCCCEEEEEEECCCCEEEEHH
DIFTQARTKVSSFPGINGAPSFSPDGKTLAVTLSKDGQSEIYTIDIATKTTRRITNHYAI
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHEEEE
DTEPSWFPDGKSLLITSERGGRPQLYKVELDSGKLTRLTFDGEWNLGGSITPDGRTLIFV
CCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEEEECCCCCCCCEECCCCCEEEEE
NRTNGKFNIARMDLETRFMQVLTTTRLDESPSVAPNGTMVIYGTTHQGRQVLAAVSTDGR
ECCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCE
FKARLPAGQGEVKSPSWSPFL
EEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIIGKWALLIIAALSLPVKAALDIVITEGVDAARPIAVVPFVWQGTGPVPSQISDVVAS
CCCHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
DLTRSGTFKPLDALGLPQSGISTLAEFAPQKWTGINAEAAVVGAIKPYGPDQYLVTFDLI
HHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEEHHHH
DLLKAQLAGQAPLNKQDLLLDSRQTVITAAQFRQYGHRISDVVYEKLTGIRGAFLTRIAY
HHHHHHHCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VVVNHAEKAPYRLMIADYDGYNEHMLLRSPEPLMSPAWSPDGRRLAYVSFENRKAEIFVQ
HHHCCCCCCCEEEEEEECCCCCCEEEEECCCHHCCCCCCCCCCEEEEEEECCCCEEEEHH
DIFTQARTKVSSFPGINGAPSFSPDGKTLAVTLSKDGQSEIYTIDIATKTTRRITNHYAI
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHEEEE
DTEPSWFPDGKSLLITSERGGRPQLYKVELDSGKLTRLTFDGEWNLGGSITPDGRTLIFV
CCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEEEECCCCCCCCEECCCCCEEEEE
NRTNGKFNIARMDLETRFMQVLTTTRLDESPSVAPNGTMVIYGTTHQGRQVLAAVSTDGR
ECCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCE
FKARLPAGQGEVKSPSWSPFL
EEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA