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The map label for this gene is yebU

Identifier: 119775174

GI number: 119775174

Start: 2476168

End: 2477571

Strand: Direct

Name: yebU

Synonym: Sama_2039

Alternate gene names: 119775174

Gene position: 2476168-2477571 (Clockwise)

Preceding gene: 119775172

Following gene: 119775184

Centisome position: 57.5

GC content: 51.99

Gene sequence:

>1404_bases
ATGGTTCAATTAAATCAAAAATTTATCAATAGCATTGCGCAAGAAATGCCGCCACATCTGTCTATGGATGATTTCGTCCA
CTATTGTGGCTTGCCCCTGCGCCTGTCTATCCGGGTCAATACCCTGAAAATCACATCGGACGCGCTTCGTGCCATACTTG
AGCCCCGTGGCTGGCAATTTGAACCAGTGCCCTGGTGTGAAGACGGTTTTTGGGTAACAGTGCCCGACGACTGCCAGCCG
GGCAATTTAACCGAGCACTATCAGGGCCTGTTTTACATTCAGGAAGCCAGTTCCATGATGCCGCCTTGCGCACTGTTTAT
GGACAAAGAGCCAAGGCAACTCTTGCTGGACGTAGCATCGGCTCCTGGCTCAAAAACCACCCAGCTGGCCGCACTGATGC
ACAATCAGGGGCTTATCATCGCCAACGAGTATTCGGCGTCCCGCACCAAGGCATTACACGCCAATTTACAGCGAATGGGT
GTTGCCAATGTGGCCATCACCCAATTTGATGGCAGGGTTTTTGGGGCGCATTTATACGAGACGCTGGATGCGATTCAACT
GGATGCCCCCTGCAGTGGCGAAGGCACGGTTCGTAAGGATCCCTTGAGTCTCAAAAACTGGTGTCCTGACGAAATTGAAG
CCATTGCGGAACTGCAGCGTGACTTGATTGACTCTGCTTTTCAGGCACTGAAACCCGGTGGTGTGCTGGTGTACTCAACC
TGCACATTGAACCGCCGCGAGAATGAAGACGTTTGCCACTTTTTAAAAGAGCGCTACGGCGATGCTGTGGTCTTTGAATC
CCTGAATGACCTTTTTAAAGGTGCAGACAAGGCACTCACGGAAGAGGGCTTTTTACACATCTGGCCGCAAATTTACGACA
GCGAAGGCTTTTTTGTCGCCCGTATTCGAAAAACCGCCAGCGTAGCAAGAAATACGGACGACCCCAGGTTTGTGAGCAAG
TTTCCTTTTGTTGCAGCCAACCGCAAAGAGCTAAGCGCCCTTGAAGAGGCGATGTTGGCCTTGGGTGTGTCTCTGCCGGA
AGATGCTGTGATAGTGACCCGGGATGGCGAGTTTTGGTTAATGCCGGCGCCCTTAGACGGCCTGCTCACTAAAATGCGTT
TTCAACGGATTGGCATACGGCTGGCAGAAACGCAAAAGCACGGCATCAAGGTCCGCCATGAAGCAGTGATGGCGCTGCCT
TGCAATCAGATGCTGTCAATTGAACCTGAGGCGGCAAAGCAGTATCTGATGGGGCGAGATATCGCGCTGGATAACGCAGG
GAAGGCGCAGGGCGAAAAAATTCTTTCACTGCATGGTGCACCGCTGGGCATTGCCAAGCATCTGGGAAATAAGCTTAAAA
ATAGCTTGCCGAGGGATCTTTGCCGCGACAACGTGCAGAACTGA

Upstream 100 bases:

>100_bases
AGCCATACCCGATTCAGCACACCTTGTCCCCCAATTCCTCCGGCCACTGACGAGGGATTTCTGATATACTCCCGCCATTC
GCTTTAAGCTATGTCGCGTT

Downstream 100 bases:

>100_bases
AATCTTTAACCGCATGCTGTGTGGCTGCGGTGAAAAATTTTTGCTTAAAATTTCAGCAAACGCTGAAATGCTGTTCTACA
CTCAGTGACAGTGTTCTTTA

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVQLNQKFINSIAQEMPPHLSMDDFVHYCGLPLRLSIRVNTLKITSDALRAILEPRGWQFEPVPWCEDGFWVTVPDDCQP
GNLTEHYQGLFYIQEASSMMPPCALFMDKEPRQLLLDVASAPGSKTTQLAALMHNQGLIIANEYSASRTKALHANLQRMG
VANVAITQFDGRVFGAHLYETLDAIQLDAPCSGEGTVRKDPLSLKNWCPDEIEAIAELQRDLIDSAFQALKPGGVLVYST
CTLNRRENEDVCHFLKERYGDAVVFESLNDLFKGADKALTEEGFLHIWPQIYDSEGFFVARIRKTASVARNTDDPRFVSK
FPFVAANRKELSALEEAMLALGVSLPEDAVIVTRDGEFWLMPAPLDGLLTKMRFQRIGIRLAETQKHGIKVRHEAVMALP
CNQMLSIEPEAAKQYLMGRDIALDNAGKAQGEKILSLHGAPLGIAKHLGNKLKNSLPRDLCRDNVQN

Sequences:

>Translated_467_residues
MVQLNQKFINSIAQEMPPHLSMDDFVHYCGLPLRLSIRVNTLKITSDALRAILEPRGWQFEPVPWCEDGFWVTVPDDCQP
GNLTEHYQGLFYIQEASSMMPPCALFMDKEPRQLLLDVASAPGSKTTQLAALMHNQGLIIANEYSASRTKALHANLQRMG
VANVAITQFDGRVFGAHLYETLDAIQLDAPCSGEGTVRKDPLSLKNWCPDEIEAIAELQRDLIDSAFQALKPGGVLVYST
CTLNRRENEDVCHFLKERYGDAVVFESLNDLFKGADKALTEEGFLHIWPQIYDSEGFFVARIRKTASVARNTDDPRFVSK
FPFVAANRKELSALEEAMLALGVSLPEDAVIVTRDGEFWLMPAPLDGLLTKMRFQRIGIRLAETQKHGIKVRHEAVMALP
CNQMLSIEPEAAKQYLMGRDIALDNAGKAQGEKILSLHGAPLGIAKHLGNKLKNSLPRDLCRDNVQN
>Mature_467_residues
MVQLNQKFINSIAQEMPPHLSMDDFVHYCGLPLRLSIRVNTLKITSDALRAILEPRGWQFEPVPWCEDGFWVTVPDDCQP
GNLTEHYQGLFYIQEASSMMPPCALFMDKEPRQLLLDVASAPGSKTTQLAALMHNQGLIIANEYSASRTKALHANLQRMG
VANVAITQFDGRVFGAHLYETLDAIQLDAPCSGEGTVRKDPLSLKNWCPDEIEAIAELQRDLIDSAFQALKPGGVLVYST
CTLNRRENEDVCHFLKERYGDAVVFESLNDLFKGADKALTEEGFLHIWPQIYDSEGFFVARIRKTASVARNTDDPRFVSK
FPFVAANRKELSALEEAMLALGVSLPEDAVIVTRDGEFWLMPAPLDGLLTKMRFQRIGIRLAETQKHGIKVRHEAVMALP
CNQMLSIEPEAAKQYLMGRDIALDNAGKAQGEKILSLHGAPLGIAKHLGNKLKNSLPRDLCRDNVQN

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI76150625, Length=304, Percent_Identity=33.8815789473684, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI76150623, Length=304, Percent_Identity=33.8815789473684, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI32698918, Length=156, Percent_Identity=37.8205128205128, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI39995082, Length=320, Percent_Identity=27.1875, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI301336155, Length=320, Percent_Identity=27.1875, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI11545785, Length=243, Percent_Identity=28.8065843621399, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI23199998, Length=293, Percent_Identity=29.0102389078498, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI270288818, Length=293, Percent_Identity=29.0102389078498, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI8922322, Length=293, Percent_Identity=29.3515358361775, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI270288816, Length=293, Percent_Identity=29.3515358361775, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI40316918, Length=191, Percent_Identity=31.413612565445, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI87081985, Length=467, Percent_Identity=51.8201284796574, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI2367212, Length=270, Percent_Identity=27.4074074074074, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=302, Percent_Identity=33.112582781457, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25143471, Length=284, Percent_Identity=30.2816901408451, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71998419, Length=304, Percent_Identity=26.9736842105263, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI115534021, Length=241, Percent_Identity=27.8008298755187, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=330, Percent_Identity=34.2424242424242, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319447, Length=329, Percent_Identity=27.6595744680851, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI22024126, Length=305, Percent_Identity=31.4754098360656, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21355201, Length=186, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24668781, Length=225, Percent_Identity=30.2222222222222, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356513, Length=225, Percent_Identity=30.2222222222222, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920494, Length=208, Percent_Identity=27.8846153846154, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMF_SHEAM (A1S788)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927914.1
- ProteinModelPortal:   A1S788
- SMR:   A1S788
- STRING:   A1S788
- GeneID:   4604289
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2039
- NMPDR:   fig|326297.7.peg.1914
- eggNOG:   COG0144
- HOGENOM:   HBG726909
- OMA:   VANEYSA
- PhylomeDB:   A1S788
- ProtClustDB:   PRK11933
- GO:   GO:0005737
- HAMAP:   MF_01579
- InterPro:   IPR001678
- InterPro:   IPR011023
- TIGRFAMs:   TIGR00446

Pfam domain/function: PF01189 Nol1_Nop2_Fmu

EC number: =2.1.1.178

Molecular weight: Translated: 52100; Mature: 52100

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 241-241 BINDING 143-143 BINDING 170-170 BINDING 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQLNQKFINSIAQEMPPHLSMDDFVHYCGLPLRLSIRVNTLKITSDALRAILEPRGWQF
CCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEEEEECHHHHHHHHCCCCCCC
EPVPWCEDGFWVTVPDDCQPGNLTEHYQGLFYIQEASSMMPPCALFMDKEPRQLLLDVAS
CCCCCCCCCEEEEECCCCCCCCCHHHHCCEEEEECHHCCCCCEEEECCCCHHHHHHHHHC
APGSKTTQLAALMHNQGLIIANEYSASRTKALHANLQRMGVANVAITQFDGRVFGAHLYE
CCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCEEHHHHHHH
TLDAIQLDAPCSGEGTVRKDPLSLKNWCPDEIEAIAELQRDLIDSAFQALKPGGVLVYST
HHHHHEECCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
CTLNRRENEDVCHFLKERYGDAVVFESLNDLFKGADKALTEEGFLHIWPQIYDSEGFFVA
EECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCCCEEECCEEECCCCEEEE
RIRKTASVARNTDDPRFVSKFPFVAANRKELSALEEAMLALGVSLPEDAVIVTRDGEFWL
HHHHHHHHHCCCCCCHHHHHCCCEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEE
MPAPLDGLLTKMRFQRIGIRLAETQKHGIKVRHEAVMALPCNQMLSIEPEAAKQYLMGRD
ECCCHHHHHHHHHHHHHCCEEECCHHCCEEEEEEEEEEECCCHHEECCHHHHHHHHHCCC
IALDNAGKAQGEKILSLHGAPLGIAKHLGNKLKNSLPRDLCRDNVQN
EEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHCCCCC
>Mature Secondary Structure
MVQLNQKFINSIAQEMPPHLSMDDFVHYCGLPLRLSIRVNTLKITSDALRAILEPRGWQF
CCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEEEEECHHHHHHHHCCCCCCC
EPVPWCEDGFWVTVPDDCQPGNLTEHYQGLFYIQEASSMMPPCALFMDKEPRQLLLDVAS
CCCCCCCCCEEEEECCCCCCCCCHHHHCCEEEEECHHCCCCCEEEECCCCHHHHHHHHHC
APGSKTTQLAALMHNQGLIIANEYSASRTKALHANLQRMGVANVAITQFDGRVFGAHLYE
CCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCEEHHHHHHH
TLDAIQLDAPCSGEGTVRKDPLSLKNWCPDEIEAIAELQRDLIDSAFQALKPGGVLVYST
HHHHHEECCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
CTLNRRENEDVCHFLKERYGDAVVFESLNDLFKGADKALTEEGFLHIWPQIYDSEGFFVA
EECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCCCEEECCEEECCCCEEEE
RIRKTASVARNTDDPRFVSKFPFVAANRKELSALEEAMLALGVSLPEDAVIVTRDGEFWL
HHHHHHHHHCCCCCCHHHHHCCCEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEE
MPAPLDGLLTKMRFQRIGIRLAETQKHGIKVRHEAVMALPCNQMLSIEPEAAKQYLMGRD
ECCCHHHHHHHHHHHHHCCEEECCHHCCEEEEEEEEEEECCCHHEECCHHHHHHHHHCCC
IALDNAGKAQGEKILSLHGAPLGIAKHLGNKLKNSLPRDLCRDNVQN
EEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA