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The map label for this gene is metG

Identifier: 119775184

GI number: 119775184

Start: 2484933

End: 2486960

Strand: Direct

Name: metG

Synonym: Sama_2049

Alternate gene names: 119775184

Gene position: 2484933-2486960 (Clockwise)

Preceding gene: 119775174

Following gene: 119775186

Centisome position: 57.71

GC content: 54.59

Gene sequence:

>2028_bases
ATGGCCAATTCACAACGTAAAATCCTTGTCACCAGCGCCCTTCCCTATGCCAACGGCCCAATCCATTTGGGGCACATGCT
GGAATACATCCAGACCGATATCTGGTCACGTTTTCAGAAACTGCGCGGCCATGAGTGCCACTATATTTGCGCCGACGATG
CCCATGGCACGCCTATCATGCTCAAGGCACAACAGCTGGGCATGGCTCCCGAAGAGATGATTGCCCAGGTCAACGTTGAG
CATCAGCAGGACTTCGCTGACTTCAACGTGGCCTTTGACAACTATCACAGCACCCACAGCGACGAAAACCGCGCACTGGC
GAGCGAGATTTATCTGAAACTTCGTGATGGCGGCTACATCAAGAGCAAGACAATTTCTCAGCTGTTCGACCCTGAAAAAT
CGATGTTCCTGCCGGACCGCTTCGTTAAGGGCACCTGCCCCAAGTGTAAGTCTGAAGATCAGTATGGCGACAACTGCGAT
GCTTGCGGTGCAACCTACAGCCCAACCGAGCTGATTAACCCGCGCTCTGCCGTGAGTGGTGCCACCCCGGTAATGAAAGA
GACTGAGCATTTCTTCTTCGACCTGCCTGCGTTTGAAGGCATGCTCAAAGCATGGACCCGCTCAGGTGCACTGCAGTCAG
AAATGGCCAACAAGCTGGACGAATGGTTCGAACAGGGCCTGCAACAGTGGGACATCACCCGCGATGCGCCTTACTTCGGT
TTTGAAATCCCCGATGCGCCGGGCAAGTATTTTTACGTGTGGCTGGATGCCCCTATCGGCTACATGGGCTCATTCAAAAA
CTTCTGCGATAAGCGCGGCGATATCAATTTCGATGAGTTCTGGGCCAAAGACTCCAGCGCCGAGGTGTATCACTTTATCG
GCAAGGACATCGTCTACTTCCACAGCCTGTTCTGGCCAGCCATGCTGCACGGCGCAGGTTTCCGCCAGCCAAGCAGTGTT
TACGCCCACGGCTATGTGACCGTAAACGGCGCCAAGATGTCCAAATCCAAGGGCACCTTTATCAAGGCACGTACCTATCT
GGATCATCTGGATCCTGAGTACCTGCGTTACTACTACGCAGCCAAACTCAGCGCCCGTATCGACGACCTGGATTTGAACC
TGGAAGACTTTGCCCAGCGGGTGAACTCCGACTTGGTTGGCAAACTTGTTAACCTGGCCTCCCGCACCGCTGGCTTTATC
ACCAAGCGCTTCGATGGCAAGCTTGCCGCCGTGGCCGACAAGAGCCTGATTGACAGCTTCCTCGCCAAGCAGGACGTGAT
TGCTGAACTGTATGAAGCCCGCGAATACGGTAAAGCCATGCGTGAAATCATGGCGCTGGCCGACGTAGCCAACGGCTTTG
TGGCCGATGCCGCTCCATGGCAGCTGGTGAAACAGGACGACAAGCAGGAAGAAGCCCATCAGGTATGCTCCAACGCACTG
AACCTGTTCCGTATTTTGGTCACCTACTTAAAGCCTGTACTGCCACGCCTTGCCAAAGACGTGGAAGATTTCTTCAGGAT
GGAGCTGACTTGGGACGGTCTTGACAGCGATTTGAGCGGCCATGAAATTGCCCCGTTCAAGCCAATGATGCAACGGGTTG
AACTGGACAAGGTTGCCGCCATGGTGGAGGCCTCCAAGGAAAACCTGCAGGCGACTGCTGAACCGGTTAAAACAGGCCCA
CTGGCAGATGACCCTATCAGTGAAACCATCAGCTACGAAGACTTCGCTAAAATCGATTTGCGTGTGGCGCTGATTCAAAA
AGCCGAAGCCGTGCCAGAAGCCGATAAGCTGCTGAAGCTGCAGCTGGATATCGGCGGTGAAGTACGTCAGGTGTTTGCCG
GTATCAAGTCTGCCTATAATCCGGAAGATCTCGAAGGCAAGCTCACTGTGATGGTGGCTAACCTCGCACCGCGTAAGATG
CGTTTCGGCATGTCCGAAGGCATGGTGCTGGCCGCTGGCCCCGGTGGTAAAGACCTGTTTGTGCTGGAGCCACACGCGGG
CGCCAAACCAGGTATGCGGGTGAAGTAA

Upstream 100 bases:

>100_bases
GCACAAATAACTCAATTGCACAGGCATTTGGTGAAAATAACGCGCGCATCGGTTAGTATCTAAGCCAATTATTTCGGGAC
CAACACGATTTTGAGTAAAG

Downstream 100 bases:

>100_bases
GCTCAACGTTGAAATGAAAAGGCCGCAATTGCGGCCTTTTTTGTATATTCAAGCGCGTGATTGTAACGCCTTAAACCGGA
TTATCGATATCGACAAAATG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIMLKAQQLGMAPEEMIAQVNVE
HQQDFADFNVAFDNYHSTHSDENRALASEIYLKLRDGGYIKSKTISQLFDPEKSMFLPDRFVKGTCPKCKSEDQYGDNCD
ACGATYSPTELINPRSAVSGATPVMKETEHFFFDLPAFEGMLKAWTRSGALQSEMANKLDEWFEQGLQQWDITRDAPYFG
FEIPDAPGKYFYVWLDAPIGYMGSFKNFCDKRGDINFDEFWAKDSSAEVYHFIGKDIVYFHSLFWPAMLHGAGFRQPSSV
YAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYAAKLSARIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFI
TKRFDGKLAAVADKSLIDSFLAKQDVIAELYEAREYGKAMREIMALADVANGFVADAAPWQLVKQDDKQEEAHQVCSNAL
NLFRILVTYLKPVLPRLAKDVEDFFRMELTWDGLDSDLSGHEIAPFKPMMQRVELDKVAAMVEASKENLQATAEPVKTGP
LADDPISETISYEDFAKIDLRVALIQKAEAVPEADKLLKLQLDIGGEVRQVFAGIKSAYNPEDLEGKLTVMVANLAPRKM
RFGMSEGMVLAAGPGGKDLFVLEPHAGAKPGMRVK

Sequences:

>Translated_675_residues
MANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIMLKAQQLGMAPEEMIAQVNVE
HQQDFADFNVAFDNYHSTHSDENRALASEIYLKLRDGGYIKSKTISQLFDPEKSMFLPDRFVKGTCPKCKSEDQYGDNCD
ACGATYSPTELINPRSAVSGATPVMKETEHFFFDLPAFEGMLKAWTRSGALQSEMANKLDEWFEQGLQQWDITRDAPYFG
FEIPDAPGKYFYVWLDAPIGYMGSFKNFCDKRGDINFDEFWAKDSSAEVYHFIGKDIVYFHSLFWPAMLHGAGFRQPSSV
YAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYAAKLSARIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFI
TKRFDGKLAAVADKSLIDSFLAKQDVIAELYEAREYGKAMREIMALADVANGFVADAAPWQLVKQDDKQEEAHQVCSNAL
NLFRILVTYLKPVLPRLAKDVEDFFRMELTWDGLDSDLSGHEIAPFKPMMQRVELDKVAAMVEASKENLQATAEPVKTGP
LADDPISETISYEDFAKIDLRVALIQKAEAVPEADKLLKLQLDIGGEVRQVFAGIKSAYNPEDLEGKLTVMVANLAPRKM
RFGMSEGMVLAAGPGGKDLFVLEPHAGAKPGMRVK
>Mature_674_residues
ANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIMLKAQQLGMAPEEMIAQVNVEH
QQDFADFNVAFDNYHSTHSDENRALASEIYLKLRDGGYIKSKTISQLFDPEKSMFLPDRFVKGTCPKCKSEDQYGDNCDA
CGATYSPTELINPRSAVSGATPVMKETEHFFFDLPAFEGMLKAWTRSGALQSEMANKLDEWFEQGLQQWDITRDAPYFGF
EIPDAPGKYFYVWLDAPIGYMGSFKNFCDKRGDINFDEFWAKDSSAEVYHFIGKDIVYFHSLFWPAMLHGAGFRQPSSVY
AHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYAAKLSARIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFIT
KRFDGKLAAVADKSLIDSFLAKQDVIAELYEAREYGKAMREIMALADVANGFVADAAPWQLVKQDDKQEEAHQVCSNALN
LFRILVTYLKPVLPRLAKDVEDFFRMELTWDGLDSDLSGHEIAPFKPMMQRVELDKVAAMVEASKENLQATAEPVKTGPL
ADDPISETISYEDFAKIDLRVALIQKAEAVPEADKLLKLQLDIGGEVRQVFAGIKSAYNPEDLEGKLTVMVANLAPRKMR
FGMSEGMVLAAGPGGKDLFVLEPHAGAKPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=628, Percent_Identity=27.5477707006369, Blast_Score=259, Evalue=7e-69,
Organism=Homo sapiens, GI24308436, Length=516, Percent_Identity=25.5813953488372, Blast_Score=157, Evalue=4e-38,
Organism=Homo sapiens, GI4507947, Length=128, Percent_Identity=35.9375, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788432, Length=678, Percent_Identity=68.7315634218289, Blast_Score=1018, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=36.1111111111111, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540794, Length=565, Percent_Identity=28.141592920354, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI212645096, Length=516, Percent_Identity=24.4186046511628, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71992781, Length=411, Percent_Identity=24.8175182481752, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321704, Length=563, Percent_Identity=27.886323268206, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321610, Length=546, Percent_Identity=23.0769230769231, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24655311, Length=634, Percent_Identity=28.5488958990536, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI21358483, Length=517, Percent_Identity=25.1450676982592, Blast_Score=134, Evalue=3e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_SHEAM (A1S798)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927924.1
- ProteinModelPortal:   A1S798
- STRING:   A1S798
- GeneID:   4604299
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2049
- NMPDR:   fig|326297.7.peg.1924
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- PhylomeDB:   A1S798
- ProtClustDB:   PRK00133
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75707; Mature: 75576

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIM
CCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQQLGMAPEEMIAQVNVEHQQDFADFNVAFDNYHSTHSDENRALASEIYLKLRDGGYI
EEHHHHCCCHHHHHHHCCCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHEEEECCCCCC
KSKTISQLFDPEKSMFLPDRFVKGTCPKCKSEDQYGDNCDACGATYSPTELINPRSAVSG
HHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMKETEHFFFDLPAFEGMLKAWTRSGALQSEMANKLDEWFEQGLQQWDITRDAPYFG
CCCHHHCCCHHEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKYFYVWLDAPIGYMGSFKNFCDKRGDINFDEFWAKDSSAEVYHFIGKDIVYF
EECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHH
HSLFWPAMLHGAGFRQPSSVYAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYA
HHHHHHHHHHCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEEHHHHHHCCHHHHHHHHH
AKLSARIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFITKRFDGKLAAVADKSLIDSF
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LAKQDVIAELYEAREYGKAMREIMALADVANGFVADAAPWQLVKQDDKQEEAHQVCSNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
NLFRILVTYLKPVLPRLAKDVEDFFRMELTWDGLDSDLSGHEIAPFKPMMQRVELDKVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MVEASKENLQATAEPVKTGPLADDPISETISYEDFAKIDLRVALIQKAEAVPEADKLLKL
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEE
QLDIGGEVRQVFAGIKSAYNPEDLEGKLTVMVANLAPRKMRFGMSEGMVLAAGPGGKDLF
EECCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCEEE
VLEPHAGAKPGMRVK
EECCCCCCCCCCCCC
>Mature Secondary Structure 
ANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIM
CCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQQLGMAPEEMIAQVNVEHQQDFADFNVAFDNYHSTHSDENRALASEIYLKLRDGGYI
EEHHHHCCCHHHHHHHCCCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHEEEECCCCCC
KSKTISQLFDPEKSMFLPDRFVKGTCPKCKSEDQYGDNCDACGATYSPTELINPRSAVSG
HHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMKETEHFFFDLPAFEGMLKAWTRSGALQSEMANKLDEWFEQGLQQWDITRDAPYFG
CCCHHHCCCHHEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKYFYVWLDAPIGYMGSFKNFCDKRGDINFDEFWAKDSSAEVYHFIGKDIVYF
EECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHH
HSLFWPAMLHGAGFRQPSSVYAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYA
HHHHHHHHHHCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEEHHHHHHCCHHHHHHHHH
AKLSARIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFITKRFDGKLAAVADKSLIDSF
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LAKQDVIAELYEAREYGKAMREIMALADVANGFVADAAPWQLVKQDDKQEEAHQVCSNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
NLFRILVTYLKPVLPRLAKDVEDFFRMELTWDGLDSDLSGHEIAPFKPMMQRVELDKVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MVEASKENLQATAEPVKTGPLADDPISETISYEDFAKIDLRVALIQKAEAVPEADKLLKL
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEE
QLDIGGEVRQVFAGIKSAYNPEDLEGKLTVMVANLAPRKMRFGMSEGMVLAAGPGGKDLF
EECCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCEEE
VLEPHAGAKPGMRVK
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA