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The map label for this gene is ppx [H]

Identifier: 119775147

GI number: 119775147

Start: 2445564

End: 2447072

Strand: Direct

Name: ppx [H]

Synonym: Sama_2012

Alternate gene names: 119775147

Gene position: 2445564-2447072 (Clockwise)

Preceding gene: 119775146

Following gene: 119775148

Centisome position: 56.79

GC content: 52.42

Gene sequence:

>1509_bases
TTGTCATCATCAAACCAGCGACACTTCGTTGCTATCGATATGGGTTCGAACAGCTTCCATTTGATGATTGCCCGTGAGCA
AGACGGCAGTTTGCAAATTCTGCATAAAGAAAAAGCACAGGTACAGTTGGCTCAGGGCCTGAGTCCGGAAGGCTATTTAT
CCGATGAAGCCATCGGTCGGGGCCTCGAGTGTCTGCGCAATTTCAGTCAACGGTTTTCAGGCTTGTGCGAAACCCAGGTG
AGACTGGTAGCAACCCACACCCTGAGGGTCGCCCGCAATCGAGACAAATTTTTAAAAGCGGCACTGACGGTGATCCCCTA
TCCCATCGAGGTGATTTCCGGCCACGAAGAAGCGCGACTTATCTATTCGGGCATCGCCCAAAGCCAAGTGCTCGCCAAAC
ATAACCTGATTATCGATATCGGCGGTGGCTCCACCGAGGTGGTGATTGGTGAAAAGAATCAGCCTGTAATGCTCTCAAGC
CTTCGTTGTGGTTGCGTGAGCTATAACGAGCGCTTCTTTTCCGATGGTCAGCTCACCCTAAGTGCCTTTCGCGCTGCGCA
AACTGCCGCCGATAAGCAGTTTTCATCACTCTCAAAAGACTATTTCGATGGTCGTTGGGACTTGGTGCTGGGCAGCTCGG
GGTCAGTCAAAGCCATCTGTGAAGCATTGCTTGAAAGTTTTGGCGATGAAACTGTCACCCTGCCAAGGCTAAAAGAGCTG
AAACTCAAGTTGGTCCAGGCAGGTCATGTGGATGCGTTGCAATTTACCAATATTGATAAAAAGCGCATCACTCTGGTGCC
TGCCGGACTTGCGATTCTTATCAGTTTTTTCCGGCGACTTGCCATAGAACAGCTGGAGCCCACCCCTGCGGCGCTGCGGG
AAGGCGTGCTGTACGAGCTTGCCCAAATCGACCAGTCACAGGATATTCGTCACCGCACGGTAGAGAGCATCGCCAACCTC
TATCATGTGGATACCAAGCACGGTGCCAGGGTGCGTAACACCGCCATGGCACTTTTTGACAGCGTGGCCGACAGCTGGCA
AATCCGTCCCTTTGCCCGGCTCCTGTCTTACGCCGCTTCATTGCATGAAATCGGCATTCACATCAATTCCCGTGCTTACC
ACAAGCACGGGGCCTACATCATCGCCAACAGTGACCTTCCCGGTTTCAGTCAGGACTTGCAGCAGGACTTGGCGATGTTA
ATCGGCAACCAGTGTAAAAAGCCGCAGTTTGATACCATCAATGCCCTGCCCGATGGTCGCCGTGAAGTACTGGTAAAACT
CTTGTGTTTGTTGCGTCTTGCAATCCTGGTAAATCTCGGCCGGGTCGCCCGTTCATTGCGCCTGAGCTGCGCTGTATTGG
ATACCAATGAGTTGGCGGTTATCCCCGAGCAAAGCGCACGGGTTGATCTGCTGCTTAAAGATTTGAAACGAGAGAAGAAA
CATTTGGGCCAGCTTGGCCTTATTCTAAGACTGGACAGCGAGCTTCCCTCTCCCGCTGACGAAGACTGA

Upstream 100 bases:

>100_bases
GCCGAGGCAGAAGCCTTAAAGAGCGAATCCGCGCCGATTGCAGAGCAAGAAGATAACAGGGATCTGCCGGTCACCAGCTG
ATCCACGAGGGAGAAACAGC

Downstream 100 bases:

>100_bases
TACATCGCCTGTTGGTATAAAGGCTCGCCACGTACCAACTGACTTGCACTCCTCACGCCACTGGCCGGCAGGCGATTCCA
TACCAGACAAACATTTAACC

Product: guanosine-5'-triphosphate,3'-diphosphate diphosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSSSNQRHFVAIDMGSNSFHLMIAREQDGSLQILHKEKAQVQLAQGLSPEGYLSDEAIGRGLECLRNFSQRFSGLCETQV
RLVATHTLRVARNRDKFLKAALTVIPYPIEVISGHEEARLIYSGIAQSQVLAKHNLIIDIGGGSTEVVIGEKNQPVMLSS
LRCGCVSYNERFFSDGQLTLSAFRAAQTAADKQFSSLSKDYFDGRWDLVLGSSGSVKAICEALLESFGDETVTLPRLKEL
KLKLVQAGHVDALQFTNIDKKRITLVPAGLAILISFFRRLAIEQLEPTPAALREGVLYELAQIDQSQDIRHRTVESIANL
YHVDTKHGARVRNTAMALFDSVADSWQIRPFARLLSYAASLHEIGIHINSRAYHKHGAYIIANSDLPGFSQDLQQDLAML
IGNQCKKPQFDTINALPDGRREVLVKLLCLLRLAILVNLGRVARSLRLSCAVLDTNELAVIPEQSARVDLLLKDLKREKK
HLGQLGLILRLDSELPSPADED

Sequences:

>Translated_502_residues
MSSSNQRHFVAIDMGSNSFHLMIAREQDGSLQILHKEKAQVQLAQGLSPEGYLSDEAIGRGLECLRNFSQRFSGLCETQV
RLVATHTLRVARNRDKFLKAALTVIPYPIEVISGHEEARLIYSGIAQSQVLAKHNLIIDIGGGSTEVVIGEKNQPVMLSS
LRCGCVSYNERFFSDGQLTLSAFRAAQTAADKQFSSLSKDYFDGRWDLVLGSSGSVKAICEALLESFGDETVTLPRLKEL
KLKLVQAGHVDALQFTNIDKKRITLVPAGLAILISFFRRLAIEQLEPTPAALREGVLYELAQIDQSQDIRHRTVESIANL
YHVDTKHGARVRNTAMALFDSVADSWQIRPFARLLSYAASLHEIGIHINSRAYHKHGAYIIANSDLPGFSQDLQQDLAML
IGNQCKKPQFDTINALPDGRREVLVKLLCLLRLAILVNLGRVARSLRLSCAVLDTNELAVIPEQSARVDLLLKDLKREKK
HLGQLGLILRLDSELPSPADED
>Mature_501_residues
SSSNQRHFVAIDMGSNSFHLMIAREQDGSLQILHKEKAQVQLAQGLSPEGYLSDEAIGRGLECLRNFSQRFSGLCETQVR
LVATHTLRVARNRDKFLKAALTVIPYPIEVISGHEEARLIYSGIAQSQVLAKHNLIIDIGGGSTEVVIGEKNQPVMLSSL
RCGCVSYNERFFSDGQLTLSAFRAAQTAADKQFSSLSKDYFDGRWDLVLGSSGSVKAICEALLESFGDETVTLPRLKELK
LKLVQAGHVDALQFTNIDKKRITLVPAGLAILISFFRRLAIEQLEPTPAALREGVLYELAQIDQSQDIRHRTVESIANLY
HVDTKHGARVRNTAMALFDSVADSWQIRPFARLLSYAASLHEIGIHINSRAYHKHGAYIIANSDLPGFSQDLQQDLAMLI
GNQCKKPQFDTINALPDGRREVLVKLLCLLRLAILVNLGRVARSLRLSCAVLDTNELAVIPEQSARVDLLLKDLKREKKH
LGQLGLILRLDSELPSPADED

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=503, Percent_Identity=38.7673956262425, Blast_Score=342, Evalue=4e-95,
Organism=Escherichia coli, GI48994966, Length=432, Percent_Identity=39.1203703703704, Blast_Score=308, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 55649; Mature: 55518

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSNQRHFVAIDMGSNSFHLMIAREQDGSLQILHKEKAQVQLAQGLSPEGYLSDEAIGR
CCCCCCEEEEEEEECCCCEEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHH
GLECLRNFSQRFSGLCETQVRLVATHTLRVARNRDKFLKAALTVIPYPIEVISGHEEARL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHCCCHHHHH
IYSGIAQSQVLAKHNLIIDIGGGSTEVVIGEKNQPVMLSSLRCGCVSYNERFFSDGQLTL
HHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCEEEECCCCCCCCCCHHHCCCCCHHH
SAFRAAQTAADKQFSSLSKDYFDGRWDLVLGSSGSVKAICEALLESFGDETVTLPRLKEL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEECCHHHHH
KLKLVQAGHVDALQFTNIDKKRITLVPAGLAILISFFRRLAIEQLEPTPAALREGVLYEL
HHHHHHCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AQIDQSQDIRHRTVESIANLYHVDTKHGARVRNTAMALFDSVADSWQIRPFARLLSYAAS
HHCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LHEIGIHINSRAYHKHGAYIIANSDLPGFSQDLQQDLAMLIGNQCKKPQFDTINALPDGR
HHHHCEEECCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
REVLVKLLCLLRLAILVNLGRVARSLRLSCAVLDTNELAVIPEQSARVDLLLKDLKREKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCHHHHHHHHHHHHHH
HLGQLGLILRLDSELPSPADED
HHCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SSSNQRHFVAIDMGSNSFHLMIAREQDGSLQILHKEKAQVQLAQGLSPEGYLSDEAIGR
CCCCCEEEEEEEECCCCEEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHH
GLECLRNFSQRFSGLCETQVRLVATHTLRVARNRDKFLKAALTVIPYPIEVISGHEEARL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHCCCHHHHH
IYSGIAQSQVLAKHNLIIDIGGGSTEVVIGEKNQPVMLSSLRCGCVSYNERFFSDGQLTL
HHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCEEEECCCCCCCCCCHHHCCCCCHHH
SAFRAAQTAADKQFSSLSKDYFDGRWDLVLGSSGSVKAICEALLESFGDETVTLPRLKEL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEECCHHHHH
KLKLVQAGHVDALQFTNIDKKRITLVPAGLAILISFFRRLAIEQLEPTPAALREGVLYEL
HHHHHHCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AQIDQSQDIRHRTVESIANLYHVDTKHGARVRNTAMALFDSVADSWQIRPFARLLSYAAS
HHCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LHEIGIHINSRAYHKHGAYIIANSDLPGFSQDLQQDLAMLIGNQCKKPQFDTINALPDGR
HHHHCEEECCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
REVLVKLLCLLRLAILVNLGRVARSLRLSCAVLDTNELAVIPEQSARVDLLLKDLKREKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCHHHHHHHHHHHHHH
HLGQLGLILRLDSELPSPADED
HHCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]