Click here to switch to the map view.

The map label for this gene is speE [H]

Identifier: 119775099

GI number: 119775099

Start: 2393261

End: 2394136

Strand: Reverse

Name: speE [H]

Synonym: Sama_1964

Alternate gene names: 119775099

Gene position: 2394136-2393261 (Counterclockwise)

Preceding gene: 119775100

Following gene: 119775098

Centisome position: 55.6

GC content: 52.63

Gene sequence:

>876_bases
ATGCAAGACAACAATCTATATTTCGAGACGCTGCACAGCGGCTATGGTCAATATTTCGAGATAGACAAGGTGCTCTTCGA
GCAAAAGACCAGTCAGTGGCATTTATCAATTTTTGAAAATGCCCGTTTTGGCAGGGTGATGGCCCTCAATGGCGCCATTC
AAACCACCGAAGCGGATGAATTTGTCTATCACGAAATGTTGACCCACGTGCCGGTGTTGGCACATGGACAGGTTAAAAGC
CTGCTGATTATCGGCGGTGGCGATGGCGGCATGCTCCGTGAAGTGGTGAAACACCAGGCTATTGAGCGTATCGTGATGGT
GGAAATTGATAGCGCCGTTGTGGACATGTGCAAAACCTGGTTGCCTAATCATTCTGCCGGCGCCTACGACGATCCACGAG
TGGAGCTGGTTATCGCCGATGGCATGGACTTTGTTGCCAACTGCGCAGAGCGGTTTGATGTGATTATTTCTGACTGTACC
GACCCGGTTGGCCCCGGCGAAGTGCTGTTCAGCTCTGATTTTTATGCGGGCTGTAAACGATGCCTTAATGAAAACGGCAT
CTTTGTGGCCCAAAATGGGGTGCCCTTTATGCAGGTTGAATCCCTGCAGGACACGGTGCGCCGTATGAGCAGTTATACCC
GGGAGTGTTGGTTTTACATGGCGGCAGTCCCAACGTACATTGGTGGTTCCATGGCATTTGCCTGGGCCACCGACAATCCT
GAGGCGCGCAAGCTTGACCTTGCAACCCTCGAGGCGCGTTTCAACGAGGCTGGGATCAGTACCCGTTACTACACGCCCGC
AGTGCATCAGGCAAGCTTTGCCTTGCCTAAATACGTCGAACAGGCGATCCGGTCAGCCATGACCGTGACCGCTTAA

Upstream 100 bases:

>100_bases
TCTAATTGGACCTTACGGCTTCCTTTTTCCGTCTTTCCTTCGAAGTGCACGGGACTGGGTATTCTACACTTTTCAAACCG
ACACCATCGGATTCAGTTAA

Downstream 100 bases:

>100_bases
TATCTCTCTATATATAGGCACAATTGAGATGAACGAATGGTCAATTGACGACGCCCGCGCCGGGTACAACGTTGCCCACT
GGAGTCAGGGTTTTTATGGG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MQDNNLYFETLHSGYGQYFEIDKVLFEQKTSQWHLSIFENARFGRVMALNGAIQTTEADEFVYHEMLTHVPVLAHGQVKS
LLIIGGGDGGMLREVVKHQAIERIVMVEIDSAVVDMCKTWLPNHSAGAYDDPRVELVIADGMDFVANCAERFDVIISDCT
DPVGPGEVLFSSDFYAGCKRCLNENGIFVAQNGVPFMQVESLQDTVRRMSSYTRECWFYMAAVPTYIGGSMAFAWATDNP
EARKLDLATLEARFNEAGISTRYYTPAVHQASFALPKYVEQAIRSAMTVTA

Sequences:

>Translated_291_residues
MQDNNLYFETLHSGYGQYFEIDKVLFEQKTSQWHLSIFENARFGRVMALNGAIQTTEADEFVYHEMLTHVPVLAHGQVKS
LLIIGGGDGGMLREVVKHQAIERIVMVEIDSAVVDMCKTWLPNHSAGAYDDPRVELVIADGMDFVANCAERFDVIISDCT
DPVGPGEVLFSSDFYAGCKRCLNENGIFVAQNGVPFMQVESLQDTVRRMSSYTRECWFYMAAVPTYIGGSMAFAWATDNP
EARKLDLATLEARFNEAGISTRYYTPAVHQASFALPKYVEQAIRSAMTVTA
>Mature_291_residues
MQDNNLYFETLHSGYGQYFEIDKVLFEQKTSQWHLSIFENARFGRVMALNGAIQTTEADEFVYHEMLTHVPVLAHGQVKS
LLIIGGGDGGMLREVVKHQAIERIVMVEIDSAVVDMCKTWLPNHSAGAYDDPRVELVIADGMDFVANCAERFDVIISDCT
DPVGPGEVLFSSDFYAGCKRCLNENGIFVAQNGVPFMQVESLQDTVRRMSSYTRECWFYMAAVPTYIGGSMAFAWATDNP
EARKLDLATLEARFNEAGISTRYYTPAVHQASFALPKYVEQAIRSAMTVTA

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=274, Percent_Identity=32.1167883211679, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI1786312, Length=285, Percent_Identity=59.6491228070175, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25154106, Length=277, Percent_Identity=37.5451263537906, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI212645616, Length=220, Percent_Identity=40.4545454545455, Blast_Score=168, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6323175, Length=280, Percent_Identity=36.7857142857143, Blast_Score=201, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6325326, Length=275, Percent_Identity=32.7272727272727, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24645443, Length=283, Percent_Identity=35.3356890459364, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI21355941, Length=217, Percent_Identity=35.4838709677419, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32519; Mature: 32519

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNNLYFETLHSGYGQYFEIDKVLFEQKTSQWHLSIFENARFGRVMALNGAIQTTEADE
CCCCCEEHEEHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCEECCCHHH
FVYHEMLTHVPVLAHGQVKSLLIIGGGDGGMLREVVKHQAIERIVMVEIDSAVVDMCKTW
HHHHHHHHHCCEEECCCEEEEEEEECCCCHHHHHHHHHHHHHEEEEEEECHHHHHHHHHH
LPNHSAGAYDDPRVELVIADGMDFVANCAERFDVIISDCTDPVGPGEVLFSSDFYAGCKR
CCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHH
CLNENGIFVAQNGVPFMQVESLQDTVRRMSSYTRECWFYMAAVPTYIGGSMAFAWATDNP
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
EARKLDLATLEARFNEAGISTRYYTPAVHQASFALPKYVEQAIRSAMTVTA
CCCEEHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQDNNLYFETLHSGYGQYFEIDKVLFEQKTSQWHLSIFENARFGRVMALNGAIQTTEADE
CCCCCEEHEEHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCEECCCHHH
FVYHEMLTHVPVLAHGQVKSLLIIGGGDGGMLREVVKHQAIERIVMVEIDSAVVDMCKTW
HHHHHHHHHCCEEECCCEEEEEEEECCCCHHHHHHHHHHHHHEEEEEEECHHHHHHHHHH
LPNHSAGAYDDPRVELVIADGMDFVANCAERFDVIISDCTDPVGPGEVLFSSDFYAGCKR
CCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHH
CLNENGIFVAQNGVPFMQVESLQDTVRRMSSYTRECWFYMAAVPTYIGGSMAFAWATDNP
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
EARKLDLATLEARFNEAGISTRYYTPAVHQASFALPKYVEQAIRSAMTVTA
CCCEEHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA