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The map label for this gene is hisF

Identifier: 119775082

GI number: 119775082

Start: 2374746

End: 2375525

Strand: Direct

Name: hisF

Synonym: Sama_1947

Alternate gene names: 119775082

Gene position: 2374746-2375525 (Clockwise)

Preceding gene: 119775081

Following gene: 119775083

Centisome position: 55.15

GC content: 53.59

Gene sequence:

>780_bases
ATGCTGGCCAAACGTATAGTGCCTTGCCTGGATGTGAAAGACGGCAAAGTGGTTAAGGGCGTGCAATTTCGTAACCACGA
GATTGTCGGCGATATAGTGCCGCTTGCTGCCCGCTACGCCCAAGAAGGCGCCGATGAACTGGTGTTTTATGACATCAGTG
CCAGCGCCAAAGGTGCCATAGTGGATAAATCCTGGGTAAGCCGCATTGCCGAGCGCATCGACATCCCCTTTTGTGTGGCC
GGGGGAATTAAAACTGCTGAGCAGGCGCGGGAAATTCTGGCCTTCGGCGCCGACAAAATTTCAATCAACTCACCTGCGCT
CTCCGATCCCAGCCTTATCCGCAGGCTACACGATGAATTTGGTCGCCAATGCGTGGTAGTGGGCATCGACAGCTTTTATG
ATGCAGGCAGCGGTAACTACACCGTAAAGCAATTTACCGGCGATGAAGCCGCTACCAAAGACACCCGCTGGCACACGTTA
GATTGGGTTGAAGAAGTGCAGCGCCAGGGCTGTGGCGAGATAGTGCTGAACGTGATGAATCAGGACGGTGTACGCCAGGG
ATATGATATTGAGCAGCTGCTAAAGGTGCGTGCCATCTGTGATGTGCCGCTGATTGCCTCAGGTGGCGCCGGCACCATGG
CCCACTTTGCCGAGGTCTTTACCGATGCCCGAGTCGATGCAGCCCTTGCCGCAAGCGTGTTTCATAAAGGCATCATCAAT
ATTGGTGAGCTTAAACAATTCCTTGTATCACAGGGGATTGCCATCAGGTTAACGGAGTAA

Upstream 100 bases:

>100_bases
CCCTTGATGACGTAAAGGCCGTCAAAACCTCTGGTGCCAGTGGTATCATTATCGGTAAGGCGCTGCTAACCGGCGTGTTC
AGTGCAAAGGAGGCAATTGC

Downstream 100 bases:

>100_bases
TGGCCCATGGCTGACGAAACCCTTTTAAACCGTCTCGATTGGGACAAACAAGACGGGCTCATCCCCGCCATCGTGCAAAA
CCATTTAAGCGGCAAGGTGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLAKRIVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAQEGADELVFYDISASAKGAIVDKSWVSRIAERIDIPFCVA
GGIKTAEQAREILAFGADKISINSPALSDPSLIRRLHDEFGRQCVVVGIDSFYDAGSGNYTVKQFTGDEAATKDTRWHTL
DWVEEVQRQGCGEIVLNVMNQDGVRQGYDIEQLLKVRAICDVPLIASGGAGTMAHFAEVFTDARVDAALAASVFHKGIIN
IGELKQFLVSQGIAIRLTE

Sequences:

>Translated_259_residues
MLAKRIVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAQEGADELVFYDISASAKGAIVDKSWVSRIAERIDIPFCVA
GGIKTAEQAREILAFGADKISINSPALSDPSLIRRLHDEFGRQCVVVGIDSFYDAGSGNYTVKQFTGDEAATKDTRWHTL
DWVEEVQRQGCGEIVLNVMNQDGVRQGYDIEQLLKVRAICDVPLIASGGAGTMAHFAEVFTDARVDAALAASVFHKGIIN
IGELKQFLVSQGIAIRLTE
>Mature_259_residues
MLAKRIVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAQEGADELVFYDISASAKGAIVDKSWVSRIAERIDIPFCVA
GGIKTAEQAREILAFGADKISINSPALSDPSLIRRLHDEFGRQCVVVGIDSFYDAGSGNYTVKQFTGDEAATKDTRWHTL
DWVEEVQRQGCGEIVLNVMNQDGVRQGYDIEQLLKVRAICDVPLIASGGAGTMAHFAEVFTDARVDAALAASVFHKGIIN
IGELKQFLVSQGIAIRLTE

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=74.7081712062257, Blast_Score=407, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=29.746835443038, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_SHEAM (A1S6Z6)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927822.1
- ProteinModelPortal:   A1S6Z6
- SMR:   A1S6Z6
- STRING:   A1S6Z6
- GeneID:   4604197
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1947
- NMPDR:   fig|326297.7.peg.1829
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- PhylomeDB:   A1S6Z6
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28178; Mature: 28178

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAQEGADELVFYDISASAKGAI
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCE
VDKSWVSRIAERIDIPFCVAGGIKTAEQAREILAFGADKISINSPALSDPSLIRRLHDEF
ECHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHH
GRQCVVVGIDSFYDAGSGNYTVKQFTGDEAATKDTRWHTLDWVEEVQRQGCGEIVLNVMN
CCEEEEEECHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHC
QDGVRQGYDIEQLLKVRAICDVPLIASGGAGTMAHFAEVFTDARVDAALAASVFHKGIIN
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IGELKQFLVSQGIAIRLTE
HHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MLAKRIVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAQEGADELVFYDISASAKGAI
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCE
VDKSWVSRIAERIDIPFCVAGGIKTAEQAREILAFGADKISINSPALSDPSLIRRLHDEF
ECHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHH
GRQCVVVGIDSFYDAGSGNYTVKQFTGDEAATKDTRWHTLDWVEEVQRQGCGEIVLNVMN
CCEEEEEECHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHC
QDGVRQGYDIEQLLKVRAICDVPLIASGGAGTMAHFAEVFTDARVDAALAASVFHKGIIN
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IGELKQFLVSQGIAIRLTE
HHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA