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The map label for this gene is hisH [H]

Identifier: 119775080

GI number: 119775080

Start: 2373317

End: 2374030

Strand: Direct

Name: hisH [H]

Synonym: Sama_1945

Alternate gene names: 119775080

Gene position: 2373317-2374030 (Clockwise)

Preceding gene: 119775079

Following gene: 119775081

Centisome position: 55.11

GC content: 57.42

Gene sequence:

>714_bases
ATGAGTGAGGAGGCTATTCCCAACGCTGAGACCGAGAAATCGGCGCCCGAACTGGTCATCATCGACACCGGCTGCGCCAA
TTTAAGCTCGGTGAAGTTTGCCTTTGAGCGCCTTGGAGCAAAGGTGCTGGTAAGCCCAGACAGCGAAGTGCTTCGCGCCG
CACCACGTTTGGTGCTGCCCGGCGTTGGCACAGCAGCGGCAGCCATGGATGCCCTGAGAGCCAGAGCGCTTGATAAACTG
ATCCCAACCCTGCGCCAGCCTGTGCTCGGAGTCTGCCTTGGGATGCAGCTGTTGATGACCCGCTCACACGAGCGCGGCGC
CAAGGGCGAGGATATTGATTGCCTTGATATTATCCCAGGTGAAATCCGTGAGTTGGACGCATCAGGCCACCCCCTGCCCC
ACATGGGGTGGAATCAGCTAACGGTGTCCAATCATCCCCTATTTGCAGGCATTGAGCCCGGCGAGTGGGTGTATTTTGTG
CACTCCTTCTGTGCGCCAACATCCGCAGCCACCATCGCCAGCTGCGAATACGGTGAAACCTTTAGCGCCGCCATCGCCAA
AGACAACTTTATGGGGGTACAGTTCCATCCGGAGAAAAGCGCCACGACTGGCGCCAAAATCCTCAGGAATTTTCTGGCAA
TCACCCTGGCCGACCTGGGGCTTGATGACCATTTTCGAGCGCAATCACATGCTGGCACCTTGGAGCAATCATGA

Upstream 100 bases:

>100_bases
ACCACAAGGTAGAAGCCCTGTTCAAGGTGCTTGGCCGCACCCTACGCCAGGCCATTGCCATCGAAGGTGATGCCCTGCCA
TCGTCAAAAGGAGTGCTTTG

Downstream 100 bases:

>100_bases
TCATTCCTGCAATTGACCTTATCGATGGTCAGGTGGTGCGCCTGTTTCAGGGCGATTACGCCAAAAAGACCGAATTTTCA
CTGGACCCCAAGAACCAGCT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSEEAIPNAETEKSAPELVIIDTGCANLSSVKFAFERLGAKVLVSPDSEVLRAAPRLVLPGVGTAAAAMDALRARALDKL
IPTLRQPVLGVCLGMQLLMTRSHERGAKGEDIDCLDIIPGEIRELDASGHPLPHMGWNQLTVSNHPLFAGIEPGEWVYFV
HSFCAPTSAATIASCEYGETFSAAIAKDNFMGVQFHPEKSATTGAKILRNFLAITLADLGLDDHFRAQSHAGTLEQS

Sequences:

>Translated_237_residues
MSEEAIPNAETEKSAPELVIIDTGCANLSSVKFAFERLGAKVLVSPDSEVLRAAPRLVLPGVGTAAAAMDALRARALDKL
IPTLRQPVLGVCLGMQLLMTRSHERGAKGEDIDCLDIIPGEIRELDASGHPLPHMGWNQLTVSNHPLFAGIEPGEWVYFV
HSFCAPTSAATIASCEYGETFSAAIAKDNFMGVQFHPEKSATTGAKILRNFLAITLADLGLDDHFRAQSHAGTLEQS
>Mature_236_residues
SEEAIPNAETEKSAPELVIIDTGCANLSSVKFAFERLGAKVLVSPDSEVLRAAPRLVLPGVGTAAAAMDALRARALDKLI
PTLRQPVLGVCLGMQLLMTRSHERGAKGEDIDCLDIIPGEIRELDASGHPLPHMGWNQLTVSNHPLFAGIEPGEWVYFVH
SFCAPTSAATIASCEYGETFSAAIAKDNFMGVQFHPEKSATTGAKILRNFLAITLADLGLDDHFRAQSHAGTLEQS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=53.2663316582915, Blast_Score=205, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=35.2112676056338, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 25361; Mature: 25230

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEAIPNAETEKSAPELVIIDTGCANLSSVKFAFERLGAKVLVSPDSEVLRAAPRLVLP
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHHCCCEECC
GVGTAAAAMDALRARALDKLIPTLRQPVLGVCLGMQLLMTRSHERGAKGEDIDCLDIIPG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
EIRELDASGHPLPHMGWNQLTVSNHPLFAGIEPGEWVYFVHSFCAPTSAATIASCEYGET
HHHHCCCCCCCCCCCCCCEEEECCCCEEEECCCCCEEEEEHHHHCCCCCHHEECCCCCCH
FSAAIAKDNFMGVQFHPEKSATTGAKILRNFLAITLADLGLDDHFRAQSHAGTLEQS
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEEAIPNAETEKSAPELVIIDTGCANLSSVKFAFERLGAKVLVSPDSEVLRAAPRLVLP
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHHCCCEECC
GVGTAAAAMDALRARALDKLIPTLRQPVLGVCLGMQLLMTRSHERGAKGEDIDCLDIIPG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
EIRELDASGHPLPHMGWNQLTVSNHPLFAGIEPGEWVYFVHSFCAPTSAATIASCEYGET
HHHHCCCCCCCCCCCCCCEEEECCCCEEEECCCCCEEEEEHHHHCCCCCHHEECCCCCCH
FSAAIAKDNFMGVQFHPEKSATTGAKILRNFLAITLADLGLDDHFRAQSHAGTLEQS
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA