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The map label for this gene is ddl

Identifier: 119775038

GI number: 119775038

Start: 2327736

End: 2328746

Strand: Direct

Name: ddl

Synonym: Sama_1903

Alternate gene names: 119775038

Gene position: 2327736-2328746 (Clockwise)

Preceding gene: 119775037

Following gene: 119775039

Centisome position: 54.06

GC content: 54.7

Gene sequence:

>1011_bases
ATGAACAAATACAATCTGGTGCTGCTCTGTGGTGGCGGTGGCAGCGAACACGATATCTCTTTGCTTTCGGCCAAATACTT
CGAGTCCTGCCTGGCTACCCTGCCCCGTTTCAATACCCTGTGGCTTGAGCTGACCGCTGAAGGCCAATTCCGCACCCGTG
AAGGTGAGATTGTAGAGCTGACCAACCGCCGTGAAATTCGTTTCGCCGACGAGGCAAAAGCCCCCTGGTCAGTGGATTAT
GTCATTCCCTGCATTCACGGCTACCCAGGCGAGACCGGCGATATTCAGTCATGGTTCAATCTTATCCGCCTGCCCTATTT
TGGCTGCGGCTCAGAGGCCTCCAGCAACTGCTTTAACAAGATCACCGCCAAAATGTGGTTCAGTGCCCTGGGCATACCCA
ATACGCCTTATCTGTACCTTGATGAACCAAGCAATGACGCCATCGCCAGGGTGGAAGCGGCGTTTGATGAATGGGGCAGC
GTGTTTATCAAGGCCGCGAGTCAGGGCTCATCGGTGGGCTGCTTCCCTGCCCATCGTCGTGAGGATATTCCAGGGCTTGT
CCGCAAGGCCTTCGAATACGCCCCCTTCGTGGTAGTAGAGAAAACCATCAAGGCCAGAGAGCTGGAAGTGGCGGTGTATG
ATTATCAGGGCGAAATCGTGGCGACCCGTCCCGGCGAAATCGTCTGTGCCGCCAATACCTTCTACAGCTTCGAAGAAAAA
TACGACACCAAGAGCCAGGCTGTCACCCATGTGGAAGCCCCGGACGTGGACGAAGAGACGGTTGCCGCCATTCGGGACTA
TGCGCTGCGGGCCTTTAAAGGCATGAAGCTGCGCCACCTGTCGCGCATTGATTTCTTCCTGACGGAAGACAATCAAATCC
TGCTGAACGAGATCAATACCTTTCCTGGATTGACTCAAATTTCCATGTTCCCCAAGATGCTGGCCCATCACGGCCATGAT
TTTGCGACCTTCCTGGAACAGGCCATATTGGCAGAGCTCGAAGGGCGCTGA

Upstream 100 bases:

>100_bases
AATCTCTGTCATCTTCCTTGCGCCCCCCACACGGGCTCATTACACTGCTCCTTTTGCCGGGACAAGTCTTTACGCCGGTT
TTACGTCTTTTGGAGACAAG

Downstream 100 bases:

>100_bases
TACCACAGCCTTAAATACAAGCCAGCAGAATTGCTGGCTTTTTTATTGCGTGTCGCATCTAAATTCGGGTTTTTCACACA
AGATCATCGCATTAGCCTTT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNKYNLVLLCGGGGSEHDISLLSAKYFESCLATLPRFNTLWLELTAEGQFRTREGEIVELTNRREIRFADEAKAPWSVDY
VIPCIHGYPGETGDIQSWFNLIRLPYFGCGSEASSNCFNKITAKMWFSALGIPNTPYLYLDEPSNDAIARVEAAFDEWGS
VFIKAASQGSSVGCFPAHRREDIPGLVRKAFEYAPFVVVEKTIKARELEVAVYDYQGEIVATRPGEIVCAANTFYSFEEK
YDTKSQAVTHVEAPDVDEETVAAIRDYALRAFKGMKLRHLSRIDFFLTEDNQILLNEINTFPGLTQISMFPKMLAHHGHD
FATFLEQAILAELEGR

Sequences:

>Translated_336_residues
MNKYNLVLLCGGGGSEHDISLLSAKYFESCLATLPRFNTLWLELTAEGQFRTREGEIVELTNRREIRFADEAKAPWSVDY
VIPCIHGYPGETGDIQSWFNLIRLPYFGCGSEASSNCFNKITAKMWFSALGIPNTPYLYLDEPSNDAIARVEAAFDEWGS
VFIKAASQGSSVGCFPAHRREDIPGLVRKAFEYAPFVVVEKTIKARELEVAVYDYQGEIVATRPGEIVCAANTFYSFEEK
YDTKSQAVTHVEAPDVDEETVAAIRDYALRAFKGMKLRHLSRIDFFLTEDNQILLNEINTFPGLTQISMFPKMLAHHGHD
FATFLEQAILAELEGR
>Mature_336_residues
MNKYNLVLLCGGGGSEHDISLLSAKYFESCLATLPRFNTLWLELTAEGQFRTREGEIVELTNRREIRFADEAKAPWSVDY
VIPCIHGYPGETGDIQSWFNLIRLPYFGCGSEASSNCFNKITAKMWFSALGIPNTPYLYLDEPSNDAIARVEAAFDEWGS
VFIKAASQGSSVGCFPAHRREDIPGLVRKAFEYAPFVVVEKTIKARELEVAVYDYQGEIVATRPGEIVCAANTFYSFEEK
YDTKSQAVTHVEAPDVDEETVAAIRDYALRAFKGMKLRHLSRIDFFLTEDNQILLNEINTFPGLTQISMFPKMLAHHGHD
FATFLEQAILAELEGR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=359, Percent_Identity=29.5264623955432, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1786280, Length=251, Percent_Identity=27.8884462151394, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_SHEAM (A1S6V2)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927778.1
- STRING:   A1S6V2
- GeneID:   4604153
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1903
- NMPDR:   fig|326297.7.peg.1790
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   APWPVDY
- PhylomeDB:   A1S6V2
- ProtClustDB:   PRK01966
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 37815; Mature: 37815

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYNLVLLCGGGGSEHDISLLSAKYFESCLATLPRFNTLWLELTAEGQFRTREGEIVEL
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEECCCCCEEEE
TNRREIRFADEAKAPWSVDYVIPCIHGYPGETGDIQSWFNLIRLPYFGCGSEASSNCFNK
CCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
ITAKMWFSALGIPNTPYLYLDEPSNDAIARVEAAFDEWGSVFIKAASQGSSVGCFPAHRR
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCC
EDIPGLVRKAFEYAPFVVVEKTIKARELEVAVYDYQGEIVATRPGEIVCAANTFYSFEEK
CCCHHHHHHHHHHCCEEEEECCCCCEEEEEEEEEECCCEEEECCCCEEEEECCHHHHHHH
YDTKSQAVTHVEAPDVDEETVAAIRDYALRAFKGMKLRHLSRIDFFLTEDNQILLNEINT
CCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCEEEEEHHCC
FPGLTQISMFPKMLAHHGHDFATFLEQAILAELEGR
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKYNLVLLCGGGGSEHDISLLSAKYFESCLATLPRFNTLWLELTAEGQFRTREGEIVEL
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEECCCCCEEEE
TNRREIRFADEAKAPWSVDYVIPCIHGYPGETGDIQSWFNLIRLPYFGCGSEASSNCFNK
CCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
ITAKMWFSALGIPNTPYLYLDEPSNDAIARVEAAFDEWGSVFIKAASQGSSVGCFPAHRR
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCC
EDIPGLVRKAFEYAPFVVVEKTIKARELEVAVYDYQGEIVATRPGEIVCAANTFYSFEEK
CCCHHHHHHHHHHCCEEEEECCCCCEEEEEEEEEECCCEEEECCCCEEEEECCHHHHHHH
YDTKSQAVTHVEAPDVDEETVAAIRDYALRAFKGMKLRHLSRIDFFLTEDNQILLNEINT
CCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCEEEEEHHCC
FPGLTQISMFPKMLAHHGHDFATFLEQAILAELEGR
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA