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The map label for this gene is gloB

Identifier: 119775019

GI number: 119775019

Start: 2299143

End: 2299919

Strand: Direct

Name: gloB

Synonym: Sama_1884

Alternate gene names: 119775019

Gene position: 2299143-2299919 (Clockwise)

Preceding gene: 119775016

Following gene: 119775020

Centisome position: 53.39

GC content: 51.09

Gene sequence:

>777_bases
ATGTTGGATATTCGCCCCATTCCCGCGTTCGCGGACAACTACATTTGGATGATTGTACTGCCTGATGGTGGTGCTGTTGT
GGTCGACCCAGGCGATGCAATGCCTGTGATAAACACCCTCCAACGCTTGAACCTGCAATTAACTGCGGTGCTTTTGACCC
ATCACCATAGAGATCATAATGGTGGCATAAACCAACTACGTGACTGGGCAAAGTCAAATGGGCAATCGTTCACTGTCTAT
GGCGCTGTAACCTCCGATTACAGTGACGTTCTTTGCCGTGACGGCGATACTGTCGACATTTCGGGCTTGACCAGCCCTGT
CAGAGTACTGTCGGTGCCGGGCCACACACTGGACCACCTGGCCTTCGTGGTAGACAACGCCCTTTTTTGTGGAGACACCC
TGTTCAGTGGCGGATGTGGCCGGTTATTTGAAGGTGATGCGGGTCAACTTTACGATTCACTGGCAAAGTTAGCATCTCTT
CCCGATGACACCCTCGTTTATTGTGCCCACGAATATACCCTCTCCAATCTGAGGTTTGCGCTGGCAGTGGAGCCACAAAA
TCAATGTCTTCAGGATTACGTTAAGCAGGTGAACAACCTACGTGAAGCCAACAAGCCTTCACTGCCAAGCACCCTTGGAA
CCGAGAAGGCAATCAACCCTTTTTTGAGGGTAGATACAGCAACTGTTCACGCCGCTGTCGCGGCACAAGCTGGACAAAAA
GTGCCAGATGAAGTGACCTGCCTCGCCCTTTTGCGCCGTTGGAAAGACAATTTTTAA

Upstream 100 bases:

>100_bases
GACTCTGTTTCACCTCATCCATTAATGGATTTTCACTGTACTTTAATTACGCAGACATGACACACTCACGCCCAGCATTT
TTCACCACAAGAGCCAAAGC

Downstream 100 bases:

>100_bases
CTTTTGCGCTACTATGTCGGCCATTTTGCTTATGACATGGAATGACTGCATCGCCTACCCGGCGATTGTGCGGCCCGAAT
AATGACGGGAACGGCATAAA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLDIRPIPAFADNYIWMIVLPDGGAVVVDPGDAMPVINTLQRLNLQLTAVLLTHHHRDHNGGINQLRDWAKSNGQSFTVY
GAVTSDYSDVLCRDGDTVDISGLTSPVRVLSVPGHTLDHLAFVVDNALFCGDTLFSGGCGRLFEGDAGQLYDSLAKLASL
PDDTLVYCAHEYTLSNLRFALAVEPQNQCLQDYVKQVNNLREANKPSLPSTLGTEKAINPFLRVDTATVHAAVAAQAGQK
VPDEVTCLALLRRWKDNF

Sequences:

>Translated_258_residues
MLDIRPIPAFADNYIWMIVLPDGGAVVVDPGDAMPVINTLQRLNLQLTAVLLTHHHRDHNGGINQLRDWAKSNGQSFTVY
GAVTSDYSDVLCRDGDTVDISGLTSPVRVLSVPGHTLDHLAFVVDNALFCGDTLFSGGCGRLFEGDAGQLYDSLAKLASL
PDDTLVYCAHEYTLSNLRFALAVEPQNQCLQDYVKQVNNLREANKPSLPSTLGTEKAINPFLRVDTATVHAAVAAQAGQK
VPDEVTCLALLRRWKDNF
>Mature_258_residues
MLDIRPIPAFADNYIWMIVLPDGGAVVVDPGDAMPVINTLQRLNLQLTAVLLTHHHRDHNGGINQLRDWAKSNGQSFTVY
GAVTSDYSDVLCRDGDTVDISGLTSPVRVLSVPGHTLDHLAFVVDNALFCGDTLFSGGCGRLFEGDAGQLYDSLAKLASL
PDDTLVYCAHEYTLSNLRFALAVEPQNQCLQDYVKQVNNLREANKPSLPSTLGTEKAINPFLRVDTATVHAAVAAQAGQK
VPDEVTCLALLRRWKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=269, Percent_Identity=34.9442379182156, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI94538322, Length=269, Percent_Identity=34.9442379182156, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI116642887, Length=269, Percent_Identity=36.4312267657993, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI21703352, Length=269, Percent_Identity=36.4312267657993, Blast_Score=148, Evalue=4e-36,
Organism=Homo sapiens, GI14150041, Length=255, Percent_Identity=36.4705882352941, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI46361987, Length=200, Percent_Identity=37.5, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1786406, Length=259, Percent_Identity=44.7876447876448, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI1787158, Length=190, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=270, Percent_Identity=31.8518518518519, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17538952, Length=162, Percent_Identity=33.9506172839506, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320478, Length=224, Percent_Identity=26.3392857142857, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324614, Length=265, Percent_Identity=27.5471698113208, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356335, Length=272, Percent_Identity=41.5441176470588, Blast_Score=188, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24667711, Length=272, Percent_Identity=41.5441176470588, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24667703, Length=272, Percent_Identity=41.5441176470588, Blast_Score=187, Evalue=6e-48,
Organism=Drosophila melanogaster, GI221330176, Length=170, Percent_Identity=30, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_SHEAM (A1S6T3)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927759.1
- ProteinModelPortal:   A1S6T3
- SMR:   A1S6T3
- STRING:   A1S6T3
- GeneID:   4604134
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1884
- NMPDR:   fig|326297.7.peg.1772
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   A1S6T3
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28117; Mature: 28117

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIRPIPAFADNYIWMIVLPDGGAVVVDPGDAMPVINTLQRLNLQLTAVLLTHHHRDHN
CCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHCCHHHEEEEEHHCCCCCC
GGINQLRDWAKSNGQSFTVYGAVTSDYSDVLCRDGDTVDISGLTSPVRVLSVPGHTLDHL
CCHHHHHHHHHCCCCEEEEEEEECCCCHHHHCCCCCEEEECCCCCCEEEEECCCCHHHHH
AFVVDNALFCGDTLFSGGCGRLFEGDAGQLYDSLAKLASLPDDTLVYCAHEYTLSNLRFA
HHHHHCHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHEECCCEEEE
LAVEPQNQCLQDYVKQVNNLREANKPSLPSTLGTEKAINPFLRVDTATVHAAVAAQAGQK
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHEECHHHHHHHHHHHCCCC
VPDEVTCLALLRRWKDNF
CCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDIRPIPAFADNYIWMIVLPDGGAVVVDPGDAMPVINTLQRLNLQLTAVLLTHHHRDHN
CCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHCCHHHEEEEEHHCCCCCC
GGINQLRDWAKSNGQSFTVYGAVTSDYSDVLCRDGDTVDISGLTSPVRVLSVPGHTLDHL
CCHHHHHHHHHCCCCEEEEEEEECCCCHHHHCCCCCEEEECCCCCCEEEEECCCCHHHHH
AFVVDNALFCGDTLFSGGCGRLFEGDAGQLYDSLAKLASLPDDTLVYCAHEYTLSNLRFA
HHHHHCHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHEECCCEEEE
LAVEPQNQCLQDYVKQVNNLREANKPSLPSTLGTEKAINPFLRVDTATVHAAVAAQAGQK
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHEECHHHHHHHHHHHCCCC
VPDEVTCLALLRRWKDNF
CCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA