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The map label for this gene is rnhA

Identifier: 119775016

GI number: 119775016

Start: 2296821

End: 2297291

Strand: Direct

Name: rnhA

Synonym: Sama_1881

Alternate gene names: 119775016

Gene position: 2296821-2297291 (Clockwise)

Preceding gene: 119775013

Following gene: 119775019

Centisome position: 53.34

GC content: 55.41

Gene sequence:

>471_bases
ATGAGCGAACTGAAACAGATCCGTATCTACACCGACGGTTCCTGTCTGGGTAACCCCGGTCCCGGTGGATACGGCGTGGT
CATGATCTATAAGCAGCACCGCAAAGAACTCGCGGATGGCTTTGCCCTGACAACCAATAACCGTATGGAGCTGCTGGCCC
CCATAGTGGCATTGGAAAGCCTTAAGGAACCCTGCGATGTGATCCTGACCAGTGACAGCCAATATATGCGTCAGGGCATT
ACCGAATGGATCCATGGCTGGAAGAAAAAGGGCTGGGTGACTGCCAGTAAAACGCCGGTCAAGAATGTGGATCTGTGGCA
ACGCCTCGATGCCGCAGCCGCCAAGCACAAGGTGGATTGGCGCTGGGTGAAAGGCCATGCGGGCCACGCCGAAAACGAAC
GCTGTGACACTCTTGCCCGTGAAGCGGCCGAAGCAAAACCGACTCAAATTGATAAGGGCTATCAGCCCTGA

Upstream 100 bases:

>100_bases
TGCCTTTCGGCATTTGAAATGATATTCATAGGTAATTCTGGCCTTTAGGAGGCAAAAAACGGTTATACTCGCCGGGAATT
CTGACTATGGTAACAACTTG

Downstream 100 bases:

>100_bases
CAACCACTGGCATCATCCAGAGGCGATGCGGCGTATCGCCGCTAGACTACCGGCCTTTCCTGAATATTGAAAAAGTTCTG
ACAAAACTGCACAAACGCAT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSELKQIRIYTDGSCLGNPGPGGYGVVMIYKQHRKELADGFALTTNNRMELLAPIVALESLKEPCDVILTSDSQYMRQGI
TEWIHGWKKKGWVTASKTPVKNVDLWQRLDAAAAKHKVDWRWVKGHAGHAENERCDTLAREAAEAKPTQIDKGYQP

Sequences:

>Translated_156_residues
MSELKQIRIYTDGSCLGNPGPGGYGVVMIYKQHRKELADGFALTTNNRMELLAPIVALESLKEPCDVILTSDSQYMRQGI
TEWIHGWKKKGWVTASKTPVKNVDLWQRLDAAAAKHKVDWRWVKGHAGHAENERCDTLAREAAEAKPTQIDKGYQP
>Mature_155_residues
SELKQIRIYTDGSCLGNPGPGGYGVVMIYKQHRKELADGFALTTNNRMELLAPIVALESLKEPCDVILTSDSQYMRQGIT
EWIHGWKKKGWVTASKTPVKNVDLWQRLDAAAAKHKVDWRWVKGHAGHAENERCDTLAREAAEAKPTQIDKGYQP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=33.5664335664336, Blast_Score=73, Evalue=9e-14,
Organism=Escherichia coli, GI1786408, Length=152, Percent_Identity=68.4210526315789, Blast_Score=226, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=37.5886524822695, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137476, Length=148, Percent_Identity=35.8108108108108, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45552509, Length=148, Percent_Identity=35.8108108108108, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_SHEAM (A1S6T0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927756.1
- ProteinModelPortal:   A1S6T0
- SMR:   A1S6T0
- STRING:   A1S6T0
- GeneID:   4604131
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1881
- NMPDR:   fig|326297.7.peg.1770
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   A1S6T0
- ProtClustDB:   PRK00203
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17532; Mature: 17401

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELKQIRIYTDGSCLGNPGPGGYGVVMIYKQHRKELADGFALTTNNRMELLAPIVALES
CCCCCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
LKEPCDVILTSDSQYMRQGITEWIHGWKKKGWVTASKTPVKNVDLWQRLDAAAAKHKVDW
HCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCE
RWVKGHAGHAENERCDTLAREAAEAKPTQIDKGYQP
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SELKQIRIYTDGSCLGNPGPGGYGVVMIYKQHRKELADGFALTTNNRMELLAPIVALES
CCCCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
LKEPCDVILTSDSQYMRQGITEWIHGWKKKGWVTASKTPVKNVDLWQRLDAAAAKHKVDW
HCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCE
RWVKGHAGHAENERCDTLAREAAEAKPTQIDKGYQP
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA