Click here to switch to the map view.

The map label for this gene is yafJ [H]

Identifier: 119775004

GI number: 119775004

Start: 2277659

End: 2278468

Strand: Reverse

Name: yafJ [H]

Synonym: Sama_1869

Alternate gene names: 119775004

Gene position: 2278468-2277659 (Counterclockwise)

Preceding gene: 119775006

Following gene: 119775000

Centisome position: 52.91

GC content: 52.96

Gene sequence:

>810_bases
ATGTGTGAGTTATTGGCGATGAGTGCCAACGTGCCCACTGACATTGTGTTCAGTTTTACCGGGCTGGCGGAGCGTGGCGG
GGTAACTGGCCCCCATGTGGATGGCTGGGGCATTACCTTTTATGAAGGCAAGGGCAGCCGCACCTTTAAAGACGCCTGTC
CATCCAGCGAATCACACATTGCCCGCTTGATTAAGTCATACCCGATTAAAAGCGAAACCGTGATAAGCCATATCCGTCAG
GCCAACCGTGGTTGTGTTGCACTGGAAAACACCCACCCCTTTGACCGGGAGTTATGGGGCCTTAACTGGACCTATGCCCA
CAACGGTCAGCTCAGTGACTATAAAGATAAGTTTGCGGTGCGCCGTTTCCGTCCGGTTGGGGATACTGACAGTGAACTGG
CGTTTTGCTGGATATTGGAAAAGCTGGTGGCCCGCTTTGGCGATGAGCGCCCCGATGACATGTTGCCTGTGTTTGCTTAC
GTGGCAGAACTTGCCGAAGAAATTCGTGCGCTTGGGGTGTTCAATATGATCCTCTCGGAAGGCAACTACCTGATGTGCTT
TTGCAGCAACAACTTAAGCCATATCACCCGCCGCGCGCCTTTTGGCAAGGCCAAGTTGATTGATACCGATGTGGTGATTG
ATTTTGACAAGGAAACCACCCCCAATGATGTGGTTACCGTGATAGCTACCCGGCCATTAACCGATAACGAGGCCTGGAAC
ATCATGCAGCCCGGTCAATGGCAGCTGTTCTGCAAAGGCGAAGTGGTTGGCGGCGGCACATTCGTGGCAAACACGGATAC
GTGCGCATAA

Upstream 100 bases:

>100_bases
AAATGTTTAAATTTTTTCGAGCCCTTCGCCATTCCCATCAACAAAGGGCAAAGTTACAATAAGTTAGTTGTTGTATTTGT
GACCTTCAGGTGCCTTGATT

Downstream 100 bases:

>100_bases
CGCGGAATGTGCTGACACAGCATAATGAAGAAAAAAGCCGGGCATTGCCGGCTTTTTTGTTGTTCATTTCACAGCTTAAG
GGTTGGCTGCCAGCGGTGCG

Product: amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MCELLAMSANVPTDIVFSFTGLAERGGVTGPHVDGWGITFYEGKGSRTFKDACPSSESHIARLIKSYPIKSETVISHIRQ
ANRGCVALENTHPFDRELWGLNWTYAHNGQLSDYKDKFAVRRFRPVGDTDSELAFCWILEKLVARFGDERPDDMLPVFAY
VAELAEEIRALGVFNMILSEGNYLMCFCSNNLSHITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTDNEAWN
IMQPGQWQLFCKGEVVGGGTFVANTDTCA

Sequences:

>Translated_269_residues
MCELLAMSANVPTDIVFSFTGLAERGGVTGPHVDGWGITFYEGKGSRTFKDACPSSESHIARLIKSYPIKSETVISHIRQ
ANRGCVALENTHPFDRELWGLNWTYAHNGQLSDYKDKFAVRRFRPVGDTDSELAFCWILEKLVARFGDERPDDMLPVFAY
VAELAEEIRALGVFNMILSEGNYLMCFCSNNLSHITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTDNEAWN
IMQPGQWQLFCKGEVVGGGTFVANTDTCA
>Mature_269_residues
MCELLAMSANVPTDIVFSFTGLAERGGVTGPHVDGWGITFYEGKGSRTFKDACPSSESHIARLIKSYPIKSETVISHIRQ
ANRGCVALENTHPFDRELWGLNWTYAHNGQLSDYKDKFAVRRFRPVGDTDSELAFCWILEKLVARFGDERPDDMLPVFAY
VAELAEEIRALGVFNMILSEGNYLMCFCSNNLSHITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTDNEAWN
IMQPGQWQLFCKGEVVGGGTFVANTDTCA

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=256, Percent_Identity=67.96875, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29987; Mature: 29987

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLAMSANVPTDIVFSFTGLAERGGVTGPHVDGWGITFYEGKGSRTFKDACPSSESHI
CCCEEEECCCCCHHEEEEECCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCHHHH
ARLIKSYPIKSETVISHIRQANRGCVALENTHPFDRELWGLNWTYAHNGQLSDYKDKFAV
HHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCEEEEECCCCCCCHHHHHHH
RRFRPVGDTDSELAFCWILEKLVARFGDERPDDMLPVFAYVAELAEEIRALGVFNMILSE
HHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNYLMCFCSNNLSHITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTDNEAWN
CCEEEEEECCCHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCC
IMQPGQWQLFCKGEVVGGGTFVANTDTCA
CCCCCCEEEEECCEEECCCEEEECCCCCC
>Mature Secondary Structure
MCELLAMSANVPTDIVFSFTGLAERGGVTGPHVDGWGITFYEGKGSRTFKDACPSSESHI
CCCEEEECCCCCHHEEEEECCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCHHHH
ARLIKSYPIKSETVISHIRQANRGCVALENTHPFDRELWGLNWTYAHNGQLSDYKDKFAV
HHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCEEEEECCCCCCCHHHHHHH
RRFRPVGDTDSELAFCWILEKLVARFGDERPDDMLPVFAYVAELAEEIRALGVFNMILSE
HHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNYLMCFCSNNLSHITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTDNEAWN
CCEEEEEECCCHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCC
IMQPGQWQLFCKGEVVGGGTFVANTDTCA
CCCCCCEEEEECCEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]