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The map label for this gene is thiH [H]

Identifier: 119774990

GI number: 119774990

Start: 2259305

End: 2260420

Strand: Reverse

Name: thiH [H]

Synonym: Sama_1855

Alternate gene names: 119774990

Gene position: 2260420-2259305 (Counterclockwise)

Preceding gene: 119774991

Following gene: 119774989

Centisome position: 52.49

GC content: 56.0

Gene sequence:

>1116_bases
ATGAGCCAGGGGTATTTCTCTGAGGCATTTGCGCGCCTGAACCCCGATAGCTTGCGGCTTAAGCTCTACTCGGCCACAGC
ACAGGATGTGGAGGCGGCACTGCGGGCTCCGGCGGGTAACCTGAATGCGCTGCTGGCACTCTTGTCGCCTGCCGCCGAGC
CTTATCTTGAGCAGATGGCACAAAAAAGCATGCAGCTCACCCGTAAGCGCTTTGGTGCCAGTATCGGTATGTACCTGCCG
CTGTATCTATCCAATCTGTGTGCAAACGAGTGTGATTACTGCGGCTTTTCAATGAGTAACCGCATTAAGCGCAAGACCCT
TGATGAAACCGAGCTTACCCGCGAAATGGCCGCCATCAAGGCCATGGGCTATGACTCAGTGCTGCTGGTCTCCGGCGAGC
ATGAAACCAAGGTCGGCATGGGCTATTTTCGTAAGGTGTTACCCGAGGTAAAGCGTGCGTTTTCCTACGTGGCGATGGAA
GTGCAGCCCCTAGCCGAGCCGGAGTACCGCGAGCTGGTAACCCTCGGGCTTGATGCTGTGATGATTTATCAGGAAACCTA
TCAGCGAGCCACCTACGCTGAGCATCACACCCGCGGCAAAAAAATGGACTTTATCTGGCGCCTGGATACGCCCGACAGAC
TGGCGCTGGCCGGTGTAGACAAGATTGGCCTTGGGGTGCTGCTGGGGCTTGATGACTGGCGCCTGGATGCGCTGTTGATG
GGGTATCACCTTGATTATCTTGAGCGAAAGTACTGGCGCAGCCGCTACAGTATTTCACTGCCAAGACTCAGGCCCTGCAC
CGGCGGCGTGGCACCGAAAACCGAGATAAGCGATAAAGGATTGGTGCAACTTATCTGTGCGTTCCGGTTGTTCAACGAAG
CGCTGGATATCAGCCTCTCGACACGGGAGCGGCCGGACTTTCGCGACAATCTGTTTGCCCTTGGGATCACCCAAACCAGC
GCTGGCAGTGCTACATCACCGGGAGGATACTCTGAGCCGGATACCCATTTGGATCAGTTTGAAATCAGCGATGACAGAAG
TGCAGCCGACATCGCAGCCGTGCTGAAGGCGAGGGGACTTAACCCCATCTGGAAAGACTGGGAATCCCAGTGGTAA

Upstream 100 bases:

>100_bases
AGAGCTTGCACGCCCACGAAACCAGCCCGCTGACCGGCTTTTTAAATACAGGCTTATCCACCACGGGCTTGGTTGATTCG
GGCATGGCGGAAAAGCTGTT

Downstream 100 bases:

>100_bases
TGGAGCCGTCAGTCGACGGTCTACAGTGCATTTTTGCTGGCGTTTCCCGCTTTTTTGGCTAATAATTGACCCGTTCATCA
AAAGCCATTTGTGAAACTTA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSQGYFSEAFARLNPDSLRLKLYSATAQDVEAALRAPAGNLNALLALLSPAAEPYLEQMAQKSMQLTRKRFGASIGMYLP
LYLSNLCANECDYCGFSMSNRIKRKTLDETELTREMAAIKAMGYDSVLLVSGEHETKVGMGYFRKVLPEVKRAFSYVAME
VQPLAEPEYRELVTLGLDAVMIYQETYQRATYAEHHTRGKKMDFIWRLDTPDRLALAGVDKIGLGVLLGLDDWRLDALLM
GYHLDYLERKYWRSRYSISLPRLRPCTGGVAPKTEISDKGLVQLICAFRLFNEALDISLSTRERPDFRDNLFALGITQTS
AGSATSPGGYSEPDTHLDQFEISDDRSAADIAAVLKARGLNPIWKDWESQW

Sequences:

>Translated_371_residues
MSQGYFSEAFARLNPDSLRLKLYSATAQDVEAALRAPAGNLNALLALLSPAAEPYLEQMAQKSMQLTRKRFGASIGMYLP
LYLSNLCANECDYCGFSMSNRIKRKTLDETELTREMAAIKAMGYDSVLLVSGEHETKVGMGYFRKVLPEVKRAFSYVAME
VQPLAEPEYRELVTLGLDAVMIYQETYQRATYAEHHTRGKKMDFIWRLDTPDRLALAGVDKIGLGVLLGLDDWRLDALLM
GYHLDYLERKYWRSRYSISLPRLRPCTGGVAPKTEISDKGLVQLICAFRLFNEALDISLSTRERPDFRDNLFALGITQTS
AGSATSPGGYSEPDTHLDQFEISDDRSAADIAAVLKARGLNPIWKDWESQW
>Mature_370_residues
SQGYFSEAFARLNPDSLRLKLYSATAQDVEAALRAPAGNLNALLALLSPAAEPYLEQMAQKSMQLTRKRFGASIGMYLPL
YLSNLCANECDYCGFSMSNRIKRKTLDETELTREMAAIKAMGYDSVLLVSGEHETKVGMGYFRKVLPEVKRAFSYVAMEV
QPLAEPEYRELVTLGLDAVMIYQETYQRATYAEHHTRGKKMDFIWRLDTPDRLALAGVDKIGLGVLLGLDDWRLDALLMG
YHLDYLERKYWRSRYSISLPRLRPCTGGVAPKTEISDKGLVQLICAFRLFNEALDISLSTRERPDFRDNLFALGITQTSA
GSATSPGGYSEPDTHLDQFEISDDRSAADIAAVLKARGLNPIWKDWESQW

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=365, Percent_Identity=57.5342465753425, Blast_Score=449, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41760; Mature: 41629

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQGYFSEAFARLNPDSLRLKLYSATAQDVEAALRAPAGNLNALLALLSPAAEPYLEQMA
CCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHH
QKSMQLTRKRFGASIGMYLPLYLSNLCANECDYCGFSMSNRIKRKTLDETELTREMAAIK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
AMGYDSVLLVSGEHETKVGMGYFRKVLPEVKRAFSYVAMEVQPLAEPEYRELVTLGLDAV
HCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
MIYQETYQRATYAEHHTRGKKMDFIWRLDTPDRLALAGVDKIGLGVLLGLDDWRLDALLM
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCHHCCEEEECCCCHHHHHHHH
GYHLDYLERKYWRSRYSISLPRLRPCTGGVAPKTEISDKGLVQLICAFRLFNEALDISLS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEC
TRERPDFRDNLFALGITQTSAGSATSPGGYSEPDTHLDQFEISDDRSAADIAAVLKARGL
CCCCCCHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCC
NPIWKDWESQW
CHHHHHHCCCC
>Mature Secondary Structure 
SQGYFSEAFARLNPDSLRLKLYSATAQDVEAALRAPAGNLNALLALLSPAAEPYLEQMA
CCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHH
QKSMQLTRKRFGASIGMYLPLYLSNLCANECDYCGFSMSNRIKRKTLDETELTREMAAIK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
AMGYDSVLLVSGEHETKVGMGYFRKVLPEVKRAFSYVAMEVQPLAEPEYRELVTLGLDAV
HCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
MIYQETYQRATYAEHHTRGKKMDFIWRLDTPDRLALAGVDKIGLGVLLGLDDWRLDALLM
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCHHCCEEEECCCCHHHHHHHH
GYHLDYLERKYWRSRYSISLPRLRPCTGGVAPKTEISDKGLVQLICAFRLFNEALDISLS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEC
TRERPDFRDNLFALGITQTSAGSATSPGGYSEPDTHLDQFEISDDRSAADIAAVLKARGL
CCCCCCHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCC
NPIWKDWESQW
CHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]