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The map label for this gene is thiG

Identifier: 119774991

GI number: 119774991

Start: 2260417

End: 2261232

Strand: Reverse

Name: thiG

Synonym: Sama_1856

Alternate gene names: 119774991

Gene position: 2261232-2260417 (Counterclockwise)

Preceding gene: 119774992

Following gene: 119774990

Centisome position: 52.51

GC content: 58.33

Gene sequence:

>816_bases
ATGCTGAAAATTGCAGAAACGGAATTTGGCTCCAGGCTGTTTACCGGCACCGGCAAGTTTGCTTCGGGTAAGCTGATGCA
GGAGGCTATTGCGGCCAGCGGCAGTGAGCTTGTCACCCTCGCCATGAAGCGGGTGGATTTTAAAACCGGCAGCGATGATA
TTCTGGCGCCGCTGCTGTCGCGCGGCGTGCGTCTGTTGCCCAATACCTCCGGTGCCCGCAATGCCAAAGAAGCGATATTC
GCAGCCGAGCTTGCCCGGGAAATGCTGGGCACCGAGTGGCTGAAGCTTGAGATCCACCCGGATCCCAAGTACCTGATGCC
GGATCCCATGGAAACCTTTGCCGCCGCCAAGGAGCTCGTCAGTCAGGGCTTTAAGGTACTGCCATATGTGCATGCCGACC
CTGTGCTCTGTCGTCGCCTCGAAGAGATTGGCTGCGCAGCGGTGATGCCCCTTGGCAGCCCCATAGGCTCCAATCAGGGC
TTGGTGAGCCGTGAATTTTTGAAAATCATCATTGAGCAGGCGAGCATTCCTGTGGTGGTGGATGCGGGCATTGGCGCGCC
GTCCCATGCGTGTGAGGCGATGGAGCTTGGCGCCGACGCCGTGCTGGTTAATACCGCTATTGCTTCGAGTGCCGACCCTG
TGCGTATGGCCAGAGCCTTTGCGCTGGCGGTGCAAATTGGCCGTGAAGCCTATCTGGCCGGGCTTGGCAGTAAGAGCTTG
CACGCCCACGAAACCAGCCCGCTGACCGGCTTTTTAAATACAGGCTTATCCACCACGGGCTTGGTTGATTCGGGCATGGC
GGAAAAGCTGTTATGA

Upstream 100 bases:

>100_bases
GTGCTCAACGGTGAAGTAGTACCGCGGCATAGCTGGGAACATATTCATCTTAAACACAATGATGCGGTTGAACTTTTTGC
AGCAGTGGCAGGAGGCTGAG

Downstream 100 bases:

>100_bases
GCCAGGGGTATTTCTCTGAGGCATTTGCGCGCCTGAACCCCGATAGCTTGCGGCTTAAGCTCTACTCGGCCACAGCACAG
GATGTGGAGGCGGCACTGCG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLKIAETEFGSRLFTGTGKFASGKLMQEAIAASGSELVTLAMKRVDFKTGSDDILAPLLSRGVRLLPNTSGARNAKEAIF
AAELAREMLGTEWLKLEIHPDPKYLMPDPMETFAAAKELVSQGFKVLPYVHADPVLCRRLEEIGCAAVMPLGSPIGSNQG
LVSREFLKIIIEQASIPVVVDAGIGAPSHACEAMELGADAVLVNTAIASSADPVRMARAFALAVQIGREAYLAGLGSKSL
HAHETSPLTGFLNTGLSTTGLVDSGMAEKLL

Sequences:

>Translated_271_residues
MLKIAETEFGSRLFTGTGKFASGKLMQEAIAASGSELVTLAMKRVDFKTGSDDILAPLLSRGVRLLPNTSGARNAKEAIF
AAELAREMLGTEWLKLEIHPDPKYLMPDPMETFAAAKELVSQGFKVLPYVHADPVLCRRLEEIGCAAVMPLGSPIGSNQG
LVSREFLKIIIEQASIPVVVDAGIGAPSHACEAMELGADAVLVNTAIASSADPVRMARAFALAVQIGREAYLAGLGSKSL
HAHETSPLTGFLNTGLSTTGLVDSGMAEKLL
>Mature_271_residues
MLKIAETEFGSRLFTGTGKFASGKLMQEAIAASGSELVTLAMKRVDFKTGSDDILAPLLSRGVRLLPNTSGARNAKEAIF
AAELAREMLGTEWLKLEIHPDPKYLMPDPMETFAAAKELVSQGFKVLPYVHADPVLCRRLEEIGCAAVMPLGSPIGSNQG
LVSREFLKIIIEQASIPVVVDAGIGAPSHACEAMELGADAVLVNTAIASSADPVRMARAFALAVQIGREAYLAGLGSKSL
HAHETSPLTGFLNTGLSTTGLVDSGMAEKLL

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=70.2380952380952, Blast_Score=360, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_SHEAM (A1S6Q5)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927731.1
- ProteinModelPortal:   A1S6Q5
- SMR:   A1S6Q5
- STRING:   A1S6Q5
- GeneID:   4604106
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1856
- NMPDR:   fig|326297.7.peg.1747
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   A1S6Q5
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28591; Mature: 28591

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIAETEFGSRLFTGTGKFASGKLMQEAIAASGSELVTLAMKRVDFKTGSDDILAPLLS
CCEECCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
RGVRLLPNTSGARNAKEAIFAAELAREMLGTEWLKLEIHPDPKYLMPDPMETFAAAKELV
CCCEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH
SQGFKVLPYVHADPVLCRRLEEIGCAAVMPLGSPIGSNQGLVSREFLKIIIEQASIPVVV
HCCCEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
DAGIGAPSHACEAMELGADAVLVNTAIASSADPVRMARAFALAVQIGREAYLAGLGSKSL
ECCCCCCHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HAHETSPLTGFLNTGLSTTGLVDSGMAEKLL
CCCCCCCCHHHHHCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MLKIAETEFGSRLFTGTGKFASGKLMQEAIAASGSELVTLAMKRVDFKTGSDDILAPLLS
CCEECCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
RGVRLLPNTSGARNAKEAIFAAELAREMLGTEWLKLEIHPDPKYLMPDPMETFAAAKELV
CCCEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH
SQGFKVLPYVHADPVLCRRLEEIGCAAVMPLGSPIGSNQGLVSREFLKIIIEQASIPVVV
HCCCEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
DAGIGAPSHACEAMELGADAVLVNTAIASSADPVRMARAFALAVQIGREAYLAGLGSKSL
ECCCCCCHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HAHETSPLTGFLNTGLSTTGLVDSGMAEKLL
CCCCCCCCHHHHHCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA