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The map label for this gene is gloA [H]

Identifier: 119774972

GI number: 119774972

Start: 2236858

End: 2237268

Strand: Direct

Name: gloA [H]

Synonym: Sama_1837

Alternate gene names: 119774972

Gene position: 2236858-2237268 (Clockwise)

Preceding gene: 119774971

Following gene: 119774980

Centisome position: 51.95

GC content: 52.55

Gene sequence:

>411_bases
ATGTCTCAAATTCTTCATACCATGGTTCGGGTTGCCAATCTTGAGAAATCCATCGCCTTCTACACTGAGGTGCTGGGCAT
GACGCTGCTTCGCACCTCAGAAAATCCTGAGTATCGTTATTCACTGGCTTTTGTGGGCTATGGCGAAGAAGCCAAGGGCG
CGGCCGTCATTGAGCTGACCTGGAACTGGGATACCGACAAGTACGAACTTGGCACTGGCTTTGGCCATATCGCCATCGGT
AAAGCAGATATTTACAAGGCCTGCGAAGACATCGCCAAGGCCGGCGGTAAAGTTACCCGCGCGCCTGGTCCTGTTGCCGG
TGGCACTACGGAAATCGCCTTCGTTGAAGACCCCGATGGTTACAAGATTGAACTCATCCAGATGAAATCTGCCATGCAAG
GCCTTGGCTGA

Upstream 100 bases:

>100_bases
GTCAAACTGTGAGTTGCTTCAGATTTCTCCTTTTGTTCCCTGTAAGCAGTACGTTTTTGCTATACTGGCCGCCATCTTAA
TGCCGTTAAAGGAACCCCAA

Downstream 100 bases:

>100_bases
TTTTACCGGAGCGACGCTGACTGAGTGAGGCTTTGACTCCCGTAATAAAAAAGCGCCCATGGGGCGCTTTTTTGTTTCTG
CGTTAAAGTGTCTGATAGTT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSQILHTMVRVANLEKSIAFYTEVLGMTLLRTSENPEYRYSLAFVGYGEEAKGAAVIELTWNWDTDKYELGTGFGHIAIG
KADIYKACEDIAKAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIELIQMKSAMQGLG

Sequences:

>Translated_136_residues
MSQILHTMVRVANLEKSIAFYTEVLGMTLLRTSENPEYRYSLAFVGYGEEAKGAAVIELTWNWDTDKYELGTGFGHIAIG
KADIYKACEDIAKAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIELIQMKSAMQGLG
>Mature_135_residues
SQILHTMVRVANLEKSIAFYTEVLGMTLLRTSENPEYRYSLAFVGYGEEAKGAAVIELTWNWDTDKYELGTGFGHIAIGK
ADIYKACEDIAKAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIELIQMKSAMQGLG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=147, Percent_Identity=35.3741496598639, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=67.910447761194, Blast_Score=185, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6323639, Length=140, Percent_Identity=39.2857142857143, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129743, Length=149, Percent_Identity=38.255033557047, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=32.6086956521739, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14770; Mature: 14639

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQILHTMVRVANLEKSIAFYTEVLGMTLLRTSENPEYRYSLAFVGYGEEAKGAAVIELT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCEEEEEEE
WNWDTDKYELGTGFGHIAIGKADIYKACEDIAKAGGKVTRAPGPVAGGTTEIAFVEDPDG
ECCCCCEEECCCCCCEEEECHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIELIQMKSAMQGLG
CEEEEEEEHHHHHCCC
>Mature Secondary Structure 
SQILHTMVRVANLEKSIAFYTEVLGMTLLRTSENPEYRYSLAFVGYGEEAKGAAVIELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCEEEEEEE
WNWDTDKYELGTGFGHIAIGKADIYKACEDIAKAGGKVTRAPGPVAGGTTEIAFVEDPDG
ECCCCCEEECCCCCCEEEECHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIELIQMKSAMQGLG
CEEEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739; 8021208 [H]