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The map label for this gene is rnfA

Identifier: 119774965

GI number: 119774965

Start: 2229685

End: 2230263

Strand: Direct

Name: rnfA

Synonym: Sama_1830

Alternate gene names: 119774965

Gene position: 2229685-2230263 (Clockwise)

Preceding gene: 119774964

Following gene: 119774966

Centisome position: 51.78

GC content: 51.99

Gene sequence:

>579_bases
ATGAGTGAATATCTCCTGTTGTTGATCGGTACTGTGCTGGTCAACAACTTCGTTTTGGTAAAATTTCTCGGACTTTGCCC
CTTTATGGGGGTGTCAGGCAAGCTTGAGTCTGCCATCGGCATGTCGATGGCGACCACCTTTGTGTTGACCCTGGCCTCCG
TTCTCAGTTTCCTGGTAAACCAGTACCTGCTGGCACCGTTTGATTTGACCTATCTGCGCACCATGAGTTTTATTCTGGTG
ATCGCTGTTGTGGTGCAATTCACCGAAATGCTGGTACAAAAAACCAGTGCCAGTCTGCACCGTGCGCTGGGTATTTACCT
GCCGCTTATCACCACCAACTGCGCCGTTCTCGGTGTGGCGCTGCTCAATGTGAACGAGCAGCACGATTTTCTGGAGTCAG
CCATCTATGGCTTTGGCGCCGCAGTGGGATTCTCTTTGGTGCTCATTCTCTTCTCAGCCATGCGTGAACGCCTTGCTGCA
GCTGATGTGCCCATGCCTTTTCGGGGTGGCGCCATTGCCATGATCACCGCTGGCCTTATGTCTCTGGCCTTTATGGGCTT
TACCGGTTTGGTGAAATAA

Upstream 100 bases:

>100_bases
CGAGAAACTCACCCGCCAGAGTTAACCCTGCATTTGATATGTTGTTAATGAGACTCAAAAAGCCGATAATACCGGCACCG
ACCGCTAATGCTTTTTTGCG

Downstream 100 bases:

>100_bases
TGTCAGCAGTCATGATAGCGGTTGTATTACTGGGCCTGCTGGCATTGGTATTTGGTGCCATATTGGGCTTTGCTGCCGTC
AAATTCAGAGTAGAAGGCGA

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSGKLESAIGMSMATTFVLTLASVLSFLVNQYLLAPFDLTYLRTMSFILV
IAVVVQFTEMLVQKTSASLHRALGIYLPLITTNCAVLGVALLNVNEQHDFLESAIYGFGAAVGFSLVLILFSAMRERLAA
ADVPMPFRGGAIAMITAGLMSLAFMGFTGLVK

Sequences:

>Translated_192_residues
MSEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSGKLESAIGMSMATTFVLTLASVLSFLVNQYLLAPFDLTYLRTMSFILV
IAVVVQFTEMLVQKTSASLHRALGIYLPLITTNCAVLGVALLNVNEQHDFLESAIYGFGAAVGFSLVLILFSAMRERLAA
ADVPMPFRGGAIAMITAGLMSLAFMGFTGLVK
>Mature_191_residues
SEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSGKLESAIGMSMATTFVLTLASVLSFLVNQYLLAPFDLTYLRTMSFILVI
AVVVQFTEMLVQKTSASLHRALGIYLPLITTNCAVLGVALLNVNEQHDFLESAIYGFGAAVGFSLVLILFSAMRERLAAA
DVPMPFRGGAIAMITAGLMSLAFMGFTGLVK

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787914, Length=192, Percent_Identity=75, Blast_Score=297, Evalue=3e-82,
Organism=Escherichia coli, GI1787919, Length=156, Percent_Identity=32.6923076923077, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFA_SHEAM (A1S6M9)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927705.1
- STRING:   A1S6M9
- GeneID:   4604080
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1830
- NMPDR:   fig|326297.7.peg.1722
- eggNOG:   COG4657
- HOGENOM:   HBG624915
- OMA:   LRTIAFI
- PhylomeDB:   A1S6M9
- ProtClustDB:   PRK05151
- GO:   GO:0006810
- HAMAP:   MF_00459
- InterPro:   IPR003667
- InterPro:   IPR011293
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01943

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 20646; Mature: 20515

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x140840e8)-; HASH(0x13efd3b0)-; HASH(0x12e2992c)-; HASH(0x12c3e638)-; HASH(0x134f6174)-; HASH(0x139c992c)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSGKLESAIGMSMATTFVLTLASVLSFLVN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYLLAPFDLTYLRTMSFILVIAVVVQFTEMLVQKTSASLHRALGIYLPLITTNCAVLGVA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNVNEQHDFLESAIYGFGAAVGFSLVLILFSAMRERLAAADVPMPFRGGAIAMITAGLM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVK
HHHHHHHHHCCH
>Mature Secondary Structure 
SEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSGKLESAIGMSMATTFVLTLASVLSFLVN
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYLLAPFDLTYLRTMSFILVIAVVVQFTEMLVQKTSASLHRALGIYLPLITTNCAVLGVA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNVNEQHDFLESAIYGFGAAVGFSLVLILFSAMRERLAAADVPMPFRGGAIAMITAGLM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVK
HHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA