Click here to switch to the map view.

The map label for this gene is pykA [H]

Identifier: 119774949

GI number: 119774949

Start: 2208391

End: 2209830

Strand: Direct

Name: pykA [H]

Synonym: Sama_1814

Alternate gene names: 119774949

Gene position: 2208391-2209830 (Clockwise)

Preceding gene: 119774948

Following gene: 119774952

Centisome position: 51.28

GC content: 54.79

Gene sequence:

>1440_bases
ATGTTCCGCAGAACCAAAATCGTTACTACCCTTGGCCCAGCCACAGACCGTGACGATAACCTGCGCCGCATCATTGCGGC
TGGTGCTAACGTAGTCAGACTTAACTTCTCCCATGGTTCACCAGAAGATCATCTCGAGCGCGCAACCCGTACCCGTGCCA
TAGCCAAAGAGCTCGGCGTGCATGTTGCTATCCTTGGTGACCTTCAGGGGCCGAAAATCCGTGTATCCACCTTTAAGGAC
AACCGTAAAGTACAGTTGGCCTTAGGTCAGGAATTTATCCTGGATGCGGAGCTGGCCAAGGGCGAAGGTGACGAAACCCA
GGTAGGTATCGACTATAAGGAGCTGCCGGACGACGTCAGCATCGGCGATATCCTGATGCTCGACGACGGTCGTGTTCAGC
TGAAAGTTGAACGCGTGGAAGGTCGCAAGGTGTTTACCACTGTGACGGTTGCCGGTCCATTGTCTAACAACAAGGGCATC
AACAAGAAAGGTGGTGGCTTGTCTGCCGCGGCACTGACCGAAAAAGACAAAGCCGATATCATCACCGCAGCCCGCATTAA
CGTTGATTACCTGGCTGTGTCTTTCCCTCGCAGCGGTGCAGACCTCAACTATGCCCGTGATCTGGCGAAAGCCGCCGGCT
CCAATGCCATGATTGTTGCCAAGGTTGAACGTGCCGAAGCTGTTGCCTCTGATGAAGCCATGGATGATGTAATCCTGGCA
TCCGACGCTGTGATGGTTGCCCGTGGCGACCTGGGCGTGGAAATCGGTGATGCAGCACTGGTAGCCGTGCAGAAAAAGCT
TATCAGTCGCTCCCGTCAGCTGAACCGCGTGGTGATCACTGCGACTCAAATGATGGAGTCGATGATTACCAGCCCAATGC
CAACCCGCGCCGAAGTGATGGACGTGGCAAACGCAGTGCTCGATGGTACTGATGCAGTGATGTTGTCTGCCGAAACCGCC
GCCGGTGACTTCCCGGAAGAAACCGTACGCGCCATGGCCAATGTGTGTGTGGGCGCAGAATCTCATCCCAGCGTAAAAGT
GTCCAAGCACCGCATGGATGAGCGTTTTACGTCGGTTGAGGAAACCATCGCGCTTTCAACCATGTACGCAGCAAACCACC
TGGACGGTGTAAAAGCCATTATCGCCCTGACTGAATCCGGAGCGACTGCACAGCTGATGAGCCGTATCAGCTCCTCATTG
CCCATCATTGCCCTGTCGCGTCACTCAACCACTCTGGCTAAGATGGCTTTGTACCGTGGTGTTCAGCCTGTGTATTTCGA
CTCTACATCCTTCAGAGCGGATGAAGTTGCTGCCCAGGCGCTCGAAGCGGTACGTGCTGCCGGTTACCTCGGCAGTGGTG
ATATGGTGCTCATGACCAAGGGTGATGCCATGGAAACCATCGGTGGTACCAACACCTGCAAGGTACTTATTCTGCCCTAA

Upstream 100 bases:

>100_bases
AAGCTTGAGATAGATCATTTTGTTTGTAACTTTCCTACATAATGCGCCAATGTCTGTTAATATAGTCTTCAGATTAACTT
GAAACTTAACGGAGTAACTC

Downstream 100 bases:

>100_bases
TGGCGGTTGGTCATCTTGTCTTTATAACCCGGCGTAAGCCGGGTTATTTTTTACCTTCTATTCTTGTGGGAATTCTTTTG
TCCAACTGCCACCGAACTCC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLERATRTRAIAKELGVHVAILGDLQGPKIRVSTFKD
NRKVQLALGQEFILDAELAKGEGDETQVGIDYKELPDDVSIGDILMLDDGRVQLKVERVEGRKVFTTVTVAGPLSNNKGI
NKKGGGLSAAALTEKDKADIITAARINVDYLAVSFPRSGADLNYARDLAKAAGSNAMIVAKVERAEAVASDEAMDDVILA
SDAVMVARGDLGVEIGDAALVAVQKKLISRSRQLNRVVITATQMMESMITSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVRAMANVCVGAESHPSVKVSKHRMDERFTSVEETIALSTMYAANHLDGVKAIIALTESGATAQLMSRISSSL
PIIALSRHSTTLAKMALYRGVQPVYFDSTSFRADEVAAQALEAVRAAGYLGSGDMVLMTKGDAMETIGGTNTCKVLILP

Sequences:

>Translated_479_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLERATRTRAIAKELGVHVAILGDLQGPKIRVSTFKD
NRKVQLALGQEFILDAELAKGEGDETQVGIDYKELPDDVSIGDILMLDDGRVQLKVERVEGRKVFTTVTVAGPLSNNKGI
NKKGGGLSAAALTEKDKADIITAARINVDYLAVSFPRSGADLNYARDLAKAAGSNAMIVAKVERAEAVASDEAMDDVILA
SDAVMVARGDLGVEIGDAALVAVQKKLISRSRQLNRVVITATQMMESMITSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVRAMANVCVGAESHPSVKVSKHRMDERFTSVEETIALSTMYAANHLDGVKAIIALTESGATAQLMSRISSSL
PIIALSRHSTTLAKMALYRGVQPVYFDSTSFRADEVAAQALEAVRAAGYLGSGDMVLMTKGDAMETIGGTNTCKVLILP
>Mature_479_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLERATRTRAIAKELGVHVAILGDLQGPKIRVSTFKD
NRKVQLALGQEFILDAELAKGEGDETQVGIDYKELPDDVSIGDILMLDDGRVQLKVERVEGRKVFTTVTVAGPLSNNKGI
NKKGGGLSAAALTEKDKADIITAARINVDYLAVSFPRSGADLNYARDLAKAAGSNAMIVAKVERAEAVASDEAMDDVILA
SDAVMVARGDLGVEIGDAALVAVQKKLISRSRQLNRVVITATQMMESMITSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVRAMANVCVGAESHPSVKVSKHRMDERFTSVEETIALSTMYAANHLDGVKAIIALTESGATAQLMSRISSSL
PIIALSRHSTTLAKMALYRGVQPVYFDSTSFRADEVAAQALEAVRAAGYLGSGDMVLMTKGDAMETIGGTNTCKVLILP

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=494, Percent_Identity=35.4251012145749, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI33286420, Length=494, Percent_Identity=35.4251012145749, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=35.2226720647773, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=34.4195519348269, Blast_Score=242, Evalue=5e-64,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=34.4195519348269, Blast_Score=242, Evalue=6e-64,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=33.9100346020761, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=33.9100346020761, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=32.3404255319149, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=32.3404255319149, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=30.3738317757009, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=72.7463312368973, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=482, Percent_Identity=36.7219917012448, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=36.3449691991786, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=493, Percent_Identity=33.6713995943205, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17506831, Length=493, Percent_Identity=33.6713995943205, Blast_Score=225, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI71984413, Length=493, Percent_Identity=33.6713995943205, Blast_Score=224, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17506829, Length=493, Percent_Identity=33.6713995943205, Blast_Score=224, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6319279, Length=474, Percent_Identity=36.2869198312236, Blast_Score=249, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=33.6734693877551, Blast_Score=229, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24648964, Length=502, Percent_Identity=33.6653386454183, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI28571814, Length=502, Percent_Identity=33.6653386454183, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24648966, Length=423, Percent_Identity=35.4609929078014, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24581235, Length=499, Percent_Identity=30.060120240481, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24646914, Length=271, Percent_Identity=35.7933579335793, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51057; Mature: 51057

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLERATRTRAIAKELGV
CCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCE
HVAILGDLQGPKIRVSTFKDNRKVQLALGQEFILDAELAKGEGDETQVGIDYKELPDDVS
EEEEEECCCCCEEEEEEECCCCEEEEEECCEEEEECHHHCCCCCCEEECCCHHHCCCCCC
IGDILMLDDGRVQLKVERVEGRKVFTTVTVAGPLSNNKGINKKGGGLSAAALTEKDKADI
CCCEEEEECCEEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCE
ITAARINVDYLAVSFPRSGADLNYARDLAKAAGSNAMIVAKVERAEAVASDEAMDDVILA
EEEEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHCCHHHCCEEEE
SDAVMVARGDLGVEIGDAALVAVQKKLISRSRQLNRVVITATQMMESMITSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVRAMANVCVGAESHPSVKVSKHRMDERFTSVE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHH
ETIALSTMYAANHLDGVKAIIALTESGATAQLMSRISSSLPIIALSRHSTTLAKMALYRG
HHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHC
VQPVYFDSTSFRADEVAAQALEAVRAAGYLGSGDMVLMTKGDAMETIGGTNTCKVLILP
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEEEC
>Mature Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLERATRTRAIAKELGV
CCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCE
HVAILGDLQGPKIRVSTFKDNRKVQLALGQEFILDAELAKGEGDETQVGIDYKELPDDVS
EEEEEECCCCCEEEEEEECCCCEEEEEECCEEEEECHHHCCCCCCEEECCCHHHCCCCCC
IGDILMLDDGRVQLKVERVEGRKVFTTVTVAGPLSNNKGINKKGGGLSAAALTEKDKADI
CCCEEEEECCEEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCE
ITAARINVDYLAVSFPRSGADLNYARDLAKAAGSNAMIVAKVERAEAVASDEAMDDVILA
EEEEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHCCHHHCCEEEE
SDAVMVARGDLGVEIGDAALVAVQKKLISRSRQLNRVVITATQMMESMITSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVRAMANVCVGAESHPSVKVSKHRMDERFTSVE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHH
ETIALSTMYAANHLDGVKAIIALTESGATAQLMSRISSSLPIIALSRHSTTLAKMALYRG
HHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHC
VQPVYFDSTSFRADEVAAQALEAVRAAGYLGSGDMVLMTKGDAMETIGGTNTCKVLILP
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]