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The map label for this gene is ribE [H]

Identifier: 119774897

GI number: 119774897

Start: 2157797

End: 2158405

Strand: Direct

Name: ribE [H]

Synonym: Sama_1762

Alternate gene names: 119774897

Gene position: 2157797-2158405 (Clockwise)

Preceding gene: 119774894

Following gene: 119774900

Centisome position: 50.11

GC content: 51.07

Gene sequence:

>609_bases
ATGTTTACTGGTATCGTGCAAGCCACCTGCGAGTTGGTGGCACTGGAAAAGCGCCGGGGAATGCACACCCTGGCGGTGAG
TCTGCCAGCTGAATTAAGAGAAGGGCTCACCCTGGGGGCAAGTGTCGCGAACAATGGCGTTTGTTTGACAGTTACCCGAG
TCGAGGATGATAGGGTCTTTTTTGACGTAATGGAAGAAACCCTTACATTAACCAACCTGGGTACGATAGCGCCGGGTGAC
CAAATTAATATTGAACGCTCTTTAACCTTTGGTAAGGAAATCGGTGGTCATCTGCTTTCCGGCCATGTGCATTGTCAGGC
GGTAGTGGAGCGAGTGACCGACGTGGGGGAGCAGAGAGATTTGCACTTACGTCTGCCGAATGAATGGATGAAATACGTTC
TTTACAAGGGGTTTGTCGCCGTCAATGGATGCAGTCTGACGGTGGGAGAGGTGTTTGATGATGGCTTTATGCTGCACCTC
ATTCCGGAAACGCTGAGGCTAACAAATCTCAAAGACGCAGTCGCCGGCACTCAACTGAATATTGAAATCGACAGTCAGAC
ACAAACCATTGTGGAGACCGTCGAGCGGGTACTGGCAGCTCGTCAGTAA

Upstream 100 bases:

>100_bases
TTGGGTAACCTGTGCTATCAGCACCGGCAAGGCCAATTGAATGAGTTTTTTGGCTTGAAAGCCCACGTTGAGCATAAAAA
ACGCCTTAAAGGAAAAATGA

Downstream 100 bases:

>100_bases
AGCTGCTCGTCATCCCCATCAACCCATAGCTCGATTTTACGATTGGCATCATCCACATGCAGTCGCATGTGGATAGGGTT
GCAACATACCCGGCAATCAT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MFTGIVQATCELVALEKRRGMHTLAVSLPAELREGLTLGASVANNGVCLTVTRVEDDRVFFDVMEETLTLTNLGTIAPGD
QINIERSLTFGKEIGGHLLSGHVHCQAVVERVTDVGEQRDLHLRLPNEWMKYVLYKGFVAVNGCSLTVGEVFDDGFMLHL
IPETLRLTNLKDAVAGTQLNIEIDSQTQTIVETVERVLAARQ

Sequences:

>Translated_202_residues
MFTGIVQATCELVALEKRRGMHTLAVSLPAELREGLTLGASVANNGVCLTVTRVEDDRVFFDVMEETLTLTNLGTIAPGD
QINIERSLTFGKEIGGHLLSGHVHCQAVVERVTDVGEQRDLHLRLPNEWMKYVLYKGFVAVNGCSLTVGEVFDDGFMLHL
IPETLRLTNLKDAVAGTQLNIEIDSQTQTIVETVERVLAARQ
>Mature_202_residues
MFTGIVQATCELVALEKRRGMHTLAVSLPAELREGLTLGASVANNGVCLTVTRVEDDRVFFDVMEETLTLTNLGTIAPGD
QINIERSLTFGKEIGGHLLSGHVHCQAVVERVTDVGEQRDLHLRLPNEWMKYVLYKGFVAVNGCSLTVGEVFDDGFMLHL
IPETLRLTNLKDAVAGTQLNIEIDSQTQTIVETVERVLAARQ

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=203, Percent_Identity=50.2463054187192, Blast_Score=202, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319733, Length=197, Percent_Identity=29.9492385786802, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22218; Mature: 22218

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVQATCELVALEKRRGMHTLAVSLPAELREGLTLGASVANNGVCLTVTRVEDDRVF
CCCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHCCCEECCEECCCCEEEEEEEECCCHHH
FDVMEETLTLTNLGTIAPGDQINIERSLTFGKEIGGHLLSGHVHCQAVVERVTDVGEQRD
HHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCC
LHLRLPNEWMKYVLYKGFVAVNGCSLTVGEVFDDGFMLHLIPETLRLTNLKDAVAGTQLN
EEEECCHHHHHHHHHHCEEEECCCEEEEHHHHCCCEEEEECHHHHHHCCCHHHHCCCEEE
IEIDSQTQTIVETVERVLAARQ
EEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFTGIVQATCELVALEKRRGMHTLAVSLPAELREGLTLGASVANNGVCLTVTRVEDDRVF
CCCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHCCCEECCEECCCCEEEEEEEECCCHHH
FDVMEETLTLTNLGTIAPGDQINIERSLTFGKEIGGHLLSGHVHCQAVVERVTDVGEQRD
HHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCC
LHLRLPNEWMKYVLYKGFVAVNGCSLTVGEVFDDGFMLHLIPETLRLTNLKDAVAGTQLN
EEEECCHHHHHHHHHHCEEEECCCEEEEHHHHCCCEEEEECHHHHHHCCCHHHHCCCEEE
IEIDSQTQTIVETVERVLAARQ
EEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9022701; 9023191; 9097039; 9278503; 11377200 [H]