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The map label for this gene is lasT [H]

Identifier: 119774888

GI number: 119774888

Start: 2144606

End: 2145283

Strand: Reverse

Name: lasT [H]

Synonym: Sama_1753

Alternate gene names: 119774888

Gene position: 2145283-2144606 (Counterclockwise)

Preceding gene: 119774889

Following gene: 119774887

Centisome position: 49.82

GC content: 55.6

Gene sequence:

>678_bases
ATGTTGTCCGTTGTACTTGTAGAACCCGCCCGCGCGGCAAACGTAGGCGCTGCGGCCCGTGCCATGAAAACCATGGGATT
TACGCAGCTGATCTTAATCGGCAGCCAGCCACATCTGGAGGATGAGGCGCGCTGGGTTGCCCATGGCGCAACAGACATAC
TCGATAATGCGCGGGTGTTGGACTCCTTCGATGACCTGCGCGCAGAGTTTGATTTGCTCATCGCCACGACCGCCAGGGAG
CGAGGCAGCCCCCGCACTTTTCTCAACCCGGAAGAACTTGCCATCACTCTTGGGCAGTCCGGCGCGCAATCCATGGCGTT
GGTGTTCGGGCGCGAGTCCAGCGGCCTATCGAATCAGGAACTCTCCCAGTGCGATCTCTTCAGCTATGTTCCCCTTGCCG
GTGACTATCCATCACTCAATCTTGGCCAGGCGGTGATGGTGTATACCTATGCCCTCAGCGGTGTTGAGCGTACGATTGGC
CTGCAGACCAGGGATGCCGATGAACGGCAGATCCAATCGCTCAAACACAAGGCCAATGAACTTGCGAACCAACTTGGACT
CTCGGAACAAGATAAACTCAGACCTTGGCTGCAAGACAGTATAGGGCGTTTGTCCGAGCGGGATTGCAAGCTGGCACATC
AACTGATAAGTGATATTATGAGCAAAATAAACGGCTGA

Upstream 100 bases:

>100_bases
CAATAAACGCATTTACCGCCTGCCTAAAAAAGCTCGGGCTAACACGGCTTTACCGATATACTCTCAACAAGATAATGCTG
TACTGGAGAGTTTAGTTGTA

Downstream 100 bases:

>100_bases
TTTTAATCGGCATTAATACGAAAAAGGAGTTTTGGATATGCTGTACCTACGCAAAAGCAGTGTGGCAATTGCCCTGATGC
TCACCCTTGGAGCCTGCTCC

Product: putative RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLSVVLVEPARAANVGAAARAMKTMGFTQLILIGSQPHLEDEARWVAHGATDILDNARVLDSFDDLRAEFDLLIATTARE
RGSPRTFLNPEELAITLGQSGAQSMALVFGRESSGLSNQELSQCDLFSYVPLAGDYPSLNLGQAVMVYTYALSGVERTIG
LQTRDADERQIQSLKHKANELANQLGLSEQDKLRPWLQDSIGRLSERDCKLAHQLISDIMSKING

Sequences:

>Translated_225_residues
MLSVVLVEPARAANVGAAARAMKTMGFTQLILIGSQPHLEDEARWVAHGATDILDNARVLDSFDDLRAEFDLLIATTARE
RGSPRTFLNPEELAITLGQSGAQSMALVFGRESSGLSNQELSQCDLFSYVPLAGDYPSLNLGQAVMVYTYALSGVERTIG
LQTRDADERQIQSLKHKANELANQLGLSEQDKLRPWLQDSIGRLSERDCKLAHQLISDIMSKING
>Mature_225_residues
MLSVVLVEPARAANVGAAARAMKTMGFTQLILIGSQPHLEDEARWVAHGATDILDNARVLDSFDDLRAEFDLLIATTARE
RGSPRTFLNPEELAITLGQSGAQSMALVFGRESSGLSNQELSQCDLFSYVPLAGDYPSLNLGQAVMVYTYALSGVERTIG
LQTRDADERQIQSLKHKANELANQLGLSEQDKLRPWLQDSIGRLSERDCKLAHQLISDIMSKING

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=225, Percent_Identity=45.3333333333333, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1788881, Length=150, Percent_Identity=38.6666666666667, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 24628; Mature: 24628

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVVLVEPARAANVGAAARAMKTMGFTQLILIGSQPHLEDEARWVAHGATDILDNARVL
CEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCHHHHHHCHHHH
DSFDDLRAEFDLLIATTARERGSPRTFLNPEELAITLGQSGAQSMALVFGRESSGLSNQE
HHHHHHHHHHHHHEEEHHHHCCCCCCCCCHHHEEEECCCCCCCCEEEEEECCCCCCCCHH
LSQCDLFSYVPLAGDYPSLNLGQAVMVYTYALSGVERTIGLQTRDADERQIQSLKHKANE
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LANQLGLSEQDKLRPWLQDSIGRLSERDCKLAHQLISDIMSKING
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSVVLVEPARAANVGAAARAMKTMGFTQLILIGSQPHLEDEARWVAHGATDILDNARVL
CEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCHHHHHHCHHHH
DSFDDLRAEFDLLIATTARERGSPRTFLNPEELAITLGQSGAQSMALVFGRESSGLSNQE
HHHHHHHHHHHHHEEEHHHHCCCCCCCCCHHHEEEECCCCCCCCEEEEEECCCCCCCCHH
LSQCDLFSYVPLAGDYPSLNLGQAVMVYTYALSGVERTIGLQTRDADERQIQSLKHKANE
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LANQLGLSEQDKLRPWLQDSIGRLSERDCKLAHQLISDIMSKING
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]