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The map label for this gene is dacB [H]

Identifier: 119774879

GI number: 119774879

Start: 2134055

End: 2135719

Strand: Reverse

Name: dacB [H]

Synonym: Sama_1744

Alternate gene names: 119774879

Gene position: 2135719-2134055 (Counterclockwise)

Preceding gene: 119774880

Following gene: 119774868

Centisome position: 49.6

GC content: 52.85

Gene sequence:

>1665_bases
ATGAAGGGTTTACAGGCTACCAAAATGACAAAGGCGCTCGTTCGCTCCCTGGCAGGACTATCGATATCATTTGCATTTGG
GGTAGGAGCCACTCCCTGGCTTTCCCTTGATGTGGAAAGCACGGTGACAGCGTCTCCTTCAGCAAGTGGTCAACCATTGG
AAGCAACTGAGGTGCCCGATAACGCGCTTGTCGAGCCGCTCTTGCCAGAACCCACGCCTATCAAAGAGGAGCGCGGCGCA
GAGCCTGCCTTTTTCGCTGATACCCCATCTTCCATCCCGCCCGCCAGTTCGCTTTCTCCGCTGAAGGATATTGCCCCTGC
CTGGTACCTTGCCATCAAGCCCAAACAAACCAACGTCGCCGTACTTGCCGTCGATCTGAAAACCCAGCAGGTCATTATCA
GCGATAACAGCGAACGCGCCATGTTGCCTGCCAGTGTGCAAAAGTTGCTGCTGGCATTGGCGGCGCAGCATACCCTCGGG
CCGGATTTTCGCTTTCATACCCAGCTTTTTGTTCAGGGGTTTAAACCTCCTGTGCTCGAGCGCCTCTATATTGGCTTTAG
CGGCGACCCACTGCTCACCAGCGCCGAGCTCAGCGCCCTGCTTGGTAGCCTTAAACAGCAAGGCGTACGCACCATTAATC
AGGTTTATCTGGTTTCTGCCGTCGATAAGAGCGATAAGGCACCCGGATGGGTTTGGGACGACCTGGGGGTTTGCTACGCC
TCACCCGTTGCCGAGTTCAGTCTCGATAAAAATTGCGTTAAGGCATCATTGCGACCAACCGAAAAAGGTGATGGCACCTA
CGTGAGATTGGCAAAAGATGCAGCAATATCCATTGAAAATCAGCTGAACTATCGACCTGGGGCCAGCTTCAAAGAATGCA
GCCCTCACCTGTCGGTTGTGGGACCTAACCGCTACCTGCTCCATGGCTGCTACAGCGGCAAACAAGCCCTTCCCTTATCC
ATTGCAGTGACAGACACAGATGCGTGGTTACAGGAAAAGGTTGCAGGCCTTTTGAAAGCTGCCGGATTCAACTCCCACAC
ACCCAAGCGTTTACATACGTTGCCTGCCAATGCCCACCTGATTGCTGACCATCAGTCGGCGACCATGGACTCCTTGCTGA
GTGAAATGCTCAAAGAGTCGGATAACCTGATTGCCGATGCGCTCCTTAAGCAATTGGGTAAGCGCAAGGGGCAGGATGAG
TCAGACTTCTATCAGGGCAGTAAGGAAATGGCAGCCAATTTGACCTTGCTCGGCGTTGACCTGTCCAGCGCCAATCTGGT
TGATGGCTCTGGCTTATCACGCTACAACCTGCTTAATGCCCGCCAATTAATGCAAACACTTCTGTTGATCCATCACACAG
AAGCATACGCCTCCCTCAAAGATGGGCTACCAGTGTCAGGAAAAGACGGCACGCTGCTTTACAAACGCGGTTTCAATTCG
CTGCCGTTAAAAGACCGGGTTCAGGCTAAAACCGGCAGTATGCAAGGTGTGTCAAACCTGGCGGGCTTTTTACGCAGTGG
TGATAGGGAATACGCTTTTGTGGTACTTGAGAACGGGTTGGTAGAGCCTGAAAGCCAAAACGGCAAACGTAAACCGGGAT
TTGCCGCCACCTTACTTAAAGCCCTGTTACAGCACGAACCACAGATCATGTCGGCACAAAAATGA

Upstream 100 bases:

>100_bases
AAAGACGATCCCCGCGATCAAAGCTGAGCGCCAATATCTGCAAGAGTCAATGGATAGAACTGCCACCGGGTTTTGGATAA
AACCTCAAAAAGGATGCAGC

Downstream 100 bases:

>100_bases
TGAAGCATGAGTATCAGAGAGCTGCTTTTGGGTGGTGCAGCACACCCAAGCTCAATAGCCCGTTTAATCAATGACAAAGA
CAAAGTAAAAAGGGCCTTCT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKGLQATKMTKALVRSLAGLSISFAFGVGATPWLSLDVESTVTASPSASGQPLEATEVPDNALVEPLLPEPTPIKEERGA
EPAFFADTPSSIPPASSLSPLKDIAPAWYLAIKPKQTNVAVLAVDLKTQQVIISDNSERAMLPASVQKLLLALAAQHTLG
PDFRFHTQLFVQGFKPPVLERLYIGFSGDPLLTSAELSALLGSLKQQGVRTINQVYLVSAVDKSDKAPGWVWDDLGVCYA
SPVAEFSLDKNCVKASLRPTEKGDGTYVRLAKDAAISIENQLNYRPGASFKECSPHLSVVGPNRYLLHGCYSGKQALPLS
IAVTDTDAWLQEKVAGLLKAAGFNSHTPKRLHTLPANAHLIADHQSATMDSLLSEMLKESDNLIADALLKQLGKRKGQDE
SDFYQGSKEMAANLTLLGVDLSSANLVDGSGLSRYNLLNARQLMQTLLLIHHTEAYASLKDGLPVSGKDGTLLYKRGFNS
LPLKDRVQAKTGSMQGVSNLAGFLRSGDREYAFVVLENGLVEPESQNGKRKPGFAATLLKALLQHEPQIMSAQK

Sequences:

>Translated_554_residues
MKGLQATKMTKALVRSLAGLSISFAFGVGATPWLSLDVESTVTASPSASGQPLEATEVPDNALVEPLLPEPTPIKEERGA
EPAFFADTPSSIPPASSLSPLKDIAPAWYLAIKPKQTNVAVLAVDLKTQQVIISDNSERAMLPASVQKLLLALAAQHTLG
PDFRFHTQLFVQGFKPPVLERLYIGFSGDPLLTSAELSALLGSLKQQGVRTINQVYLVSAVDKSDKAPGWVWDDLGVCYA
SPVAEFSLDKNCVKASLRPTEKGDGTYVRLAKDAAISIENQLNYRPGASFKECSPHLSVVGPNRYLLHGCYSGKQALPLS
IAVTDTDAWLQEKVAGLLKAAGFNSHTPKRLHTLPANAHLIADHQSATMDSLLSEMLKESDNLIADALLKQLGKRKGQDE
SDFYQGSKEMAANLTLLGVDLSSANLVDGSGLSRYNLLNARQLMQTLLLIHHTEAYASLKDGLPVSGKDGTLLYKRGFNS
LPLKDRVQAKTGSMQGVSNLAGFLRSGDREYAFVVLENGLVEPESQNGKRKPGFAATLLKALLQHEPQIMSAQK
>Mature_554_residues
MKGLQATKMTKALVRSLAGLSISFAFGVGATPWLSLDVESTVTASPSASGQPLEATEVPDNALVEPLLPEPTPIKEERGA
EPAFFADTPSSIPPASSLSPLKDIAPAWYLAIKPKQTNVAVLAVDLKTQQVIISDNSERAMLPASVQKLLLALAAQHTLG
PDFRFHTQLFVQGFKPPVLERLYIGFSGDPLLTSAELSALLGSLKQQGVRTINQVYLVSAVDKSDKAPGWVWDDLGVCYA
SPVAEFSLDKNCVKASLRPTEKGDGTYVRLAKDAAISIENQLNYRPGASFKECSPHLSVVGPNRYLLHGCYSGKQALPLS
IAVTDTDAWLQEKVAGLLKAAGFNSHTPKRLHTLPANAHLIADHQSATMDSLLSEMLKESDNLIADALLKQLGKRKGQDE
SDFYQGSKEMAANLTLLGVDLSSANLVDGSGLSRYNLLNARQLMQTLLLIHHTEAYASLKDGLPVSGKDGTLLYKRGFNS
LPLKDRVQAKTGSMQGVSNLAGFLRSGDREYAFVVLENGLVEPESQNGKRKPGFAATLLKALLQHEPQIMSAQK

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=453, Percent_Identity=35.0993377483444, Blast_Score=218, Evalue=9e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 59617; Mature: 59617

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLQATKMTKALVRSLAGLSISFAFGVGATPWLSLDVESTVTASPSASGQPLEATEVPD
CCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCC
NALVEPLLPEPTPIKEERGAEPAFFADTPSSIPPASSLSPLKDIAPAWYLAIKPKQTNVA
CCCCCCCCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCEE
VLAVDLKTQQVIISDNSERAMLPASVQKLLLALAAQHTLGPDFRFHTQLFVQGFKPPVLE
EEEEECCEEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHH
RLYIGFSGDPLLTSAELSALLGSLKQQGVRTINQVYLVSAVDKSDKAPGWVWDDLGVCYA
HEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCHHHC
SPVAEFSLDKNCVKASLRPTEKGDGTYVRLAKDAAISIENQLNYRPGASFKECSPHLSVV
CCHHHHHCCHHHHHCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCHHHCCCCEEEE
GPNRYLLHGCYSGKQALPLSIAVTDTDAWLQEKVAGLLKAAGFNSHTPKRLHTLPANAHL
CCCEEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCEE
IADHQSATMDSLLSEMLKESDNLIADALLKQLGKRKGQDESDFYQGSKEMAANLTLLGVD
EECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHCCEEEEEEE
LSSANLVDGSGLSRYNLLNARQLMQTLLLIHHTEAYASLKDGLPVSGKDGTLLYKRGFNS
CCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
LPLKDRVQAKTGSMQGVSNLAGFLRSGDREYAFVVLENGLVEPESQNGKRKPGFAATLLK
CCCHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHH
ALLQHEPQIMSAQK
HHHHCCCCHHCCCC
>Mature Secondary Structure
MKGLQATKMTKALVRSLAGLSISFAFGVGATPWLSLDVESTVTASPSASGQPLEATEVPD
CCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCC
NALVEPLLPEPTPIKEERGAEPAFFADTPSSIPPASSLSPLKDIAPAWYLAIKPKQTNVA
CCCCCCCCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCEE
VLAVDLKTQQVIISDNSERAMLPASVQKLLLALAAQHTLGPDFRFHTQLFVQGFKPPVLE
EEEEECCEEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHH
RLYIGFSGDPLLTSAELSALLGSLKQQGVRTINQVYLVSAVDKSDKAPGWVWDDLGVCYA
HEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCHHHC
SPVAEFSLDKNCVKASLRPTEKGDGTYVRLAKDAAISIENQLNYRPGASFKECSPHLSVV
CCHHHHHCCHHHHHCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCHHHCCCCEEEE
GPNRYLLHGCYSGKQALPLSIAVTDTDAWLQEKVAGLLKAAGFNSHTPKRLHTLPANAHL
CCCEEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCEE
IADHQSATMDSLLSEMLKESDNLIADALLKQLGKRKGQDESDFYQGSKEMAANLTLLGVD
EECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHCCEEEEEEE
LSSANLVDGSGLSRYNLLNARQLMQTLLLIHHTEAYASLKDGLPVSGKDGTLLYKRGFNS
CCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
LPLKDRVQAKTGSMQGVSNLAGFLRSGDREYAFVVLENGLVEPESQNGKRKPGFAATLLK
CCCHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHH
ALLQHEPQIMSAQK
HHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]