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The map label for this gene is ansA [H]

Identifier: 119774857

GI number: 119774857

Start: 2106320

End: 2107333

Strand: Reverse

Name: ansA [H]

Synonym: Sama_1722

Alternate gene names: 119774857

Gene position: 2107333-2106320 (Counterclockwise)

Preceding gene: 119774858

Following gene: 119774843

Centisome position: 48.94

GC content: 53.65

Gene sequence:

>1014_bases
ATGAAAAAGCCCTCCATTTATGTCGCCTACACCGGCGGTACCATCGGTATGCAGAAAACCGCCAATGGCTTTGCGCCCGT
TGCAGGATTTTTGACCCAGTGTGTCCAATCCATGCCGGAGTTCTATCACGAAGAAATGCCGGAGTTTGTGATCCACGAGT
ATGACCCGCTGATTGATTCATCCAACATGTCGCCGGCACATTGGCAGATGATTGCCGATGATATTCGCAACAACTATGGC
AAGTACGATGGCTTTGTCATCCTCCATGGCACAGACACCATGGCCTACACTGCGTCAGCGCTGTCCTTTATGCTGCAGGG
GTTGGGTAAGCCAGTGATAGTCACCGGCTCGCAAATCCCCCTCGCCCAGCTTCGCTCAGACGGGCAAACCAACCTGCTGA
ATGCCCTGTATATTGCCGCCCGTTATCCGGTTGCCGAAGTGTGTTTATTTTTCAATAACAAGCTATTTCGCGGCAATCGC
ACCACCAAGGCACATGCCGATGGATTTGATGCCTTCGCCTCGCCAAACTTCCCCTTGCTGCTCGAAGCGGGGATCACCAT
TAACCTGAAGGCCGGCAAAATCACCCGTGAAGCCACTGGACAGCTGGAGGTTGCCTGTATTTCACCGCAACCTGTGGGTG
TGGTGACACTGTACCCGGGTATCTCTACCGATATCTTCATCAACGTCCTGCAGCAGCCGGTGAAAGCGCTGATTTTACTG
ACCTTTGGCGTAGGCAACGCGCCTCAGGATCCAGCACTGCTTAACACACTCAAAGAAGCAGACGAACGGGGTGTGGTACT
GGTGAACCTGACGCAGTGTTTCCAGGGGAAAGTGAATATGGGCGGCTACGCAACCGGTAACGCCCTCGCCCGCGCCGGTG
TTATCAGTGGCGCCGACATGACCATCGAAGCCTGTCTCGCCAAATTGCATTTCCTGCTGTCTAAAAATCTGCCGCCGGCA
GACATAAAAGCTGCCATGCAAACCAATCTGGTGGGTGAACTCACCAGCGATTAA

Upstream 100 bases:

>100_bases
ACTTTATCAGTGAATTTTTAACTGAATTAGCCCCTGAGGTTTCGTTTAGGCGAAGGGATCCTTATAATTCGGTCAAAATC
AAACAGCAGTCACCTCACCC

Downstream 100 bases:

>100_bases
ACAAAAAAGCCGGTCTCAGACCGGCTTTTTTAGCTTTACCGCTCACTGAGCAGATCAGATTTCATCGTTACTGAAGGTCG
CGATGGTTTCACCACCGAAA

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKKPSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHEEMPEFVIHEYDPLIDSSNMSPAHWQMIADDIRNNYG
KYDGFVILHGTDTMAYTASALSFMLQGLGKPVIVTGSQIPLAQLRSDGQTNLLNALYIAARYPVAEVCLFFNNKLFRGNR
TTKAHADGFDAFASPNFPLLLEAGITINLKAGKITREATGQLEVACISPQPVGVVTLYPGISTDIFINVLQQPVKALILL
TFGVGNAPQDPALLNTLKEADERGVVLVNLTQCFQGKVNMGGYATGNALARAGVISGADMTIEACLAKLHFLLSKNLPPA
DIKAAMQTNLVGELTSD

Sequences:

>Translated_337_residues
MKKPSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHEEMPEFVIHEYDPLIDSSNMSPAHWQMIADDIRNNYG
KYDGFVILHGTDTMAYTASALSFMLQGLGKPVIVTGSQIPLAQLRSDGQTNLLNALYIAARYPVAEVCLFFNNKLFRGNR
TTKAHADGFDAFASPNFPLLLEAGITINLKAGKITREATGQLEVACISPQPVGVVTLYPGISTDIFINVLQQPVKALILL
TFGVGNAPQDPALLNTLKEADERGVVLVNLTQCFQGKVNMGGYATGNALARAGVISGADMTIEACLAKLHFLLSKNLPPA
DIKAAMQTNLVGELTSD
>Mature_337_residues
MKKPSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHEEMPEFVIHEYDPLIDSSNMSPAHWQMIADDIRNNYG
KYDGFVILHGTDTMAYTASALSFMLQGLGKPVIVTGSQIPLAQLRSDGQTNLLNALYIAARYPVAEVCLFFNNKLFRGNR
TTKAHADGFDAFASPNFPLLLEAGITINLKAGKITREATGQLEVACISPQPVGVVTLYPGISTDIFINVLQQPVKALILL
TFGVGNAPQDPALLNTLKEADERGVVLVNLTQCFQGKVNMGGYATGNALARAGVISGADMTIEACLAKLHFLLSKNLPPA
DIKAAMQTNLVGELTSD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=356, Percent_Identity=44.3820224719101, Blast_Score=254, Evalue=9e-68,
Organism=Escherichia coli, GI1788065, Length=337, Percent_Identity=68.5459940652819, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789327, Length=276, Percent_Identity=27.1739130434783, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558278, Length=357, Percent_Identity=43.6974789915966, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI193207374, Length=362, Percent_Identity=43.0939226519337, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17558280, Length=357, Percent_Identity=43.6974789915966, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI193207372, Length=362, Percent_Identity=43.0939226519337, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24639609, Length=367, Percent_Identity=43.3242506811989, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24645530, Length=363, Percent_Identity=41.0468319559229, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36252; Mature: 36252

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHEEMPEFVIHEYDPLIDS
CCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
SNMSPAHWQMIADDIRNNYGKYDGFVILHGTDTMAYTASALSFMLQGLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNALYIAARYPVAEVCLFFNNKLFRGNRTTKAHADGFDAFASPNFPLL
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCCCCCHHCCCCCCCEE
LEAGITINLKAGKITREATGQLEVACISPQPVGVVTLYPGISTDIFINVLQQPVKALILL
EECCEEEEEECCCEECCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
TFGVGNAPQDPALLNTLKEADERGVVLVNLTQCFQGKVNMGGYATGNALARAGVISGADM
EECCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEECCCHHHHHCCCCCCCH
TIEACLAKLHFLLSKNLPPADIKAAMQTNLVGELTSD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEECCCC
>Mature Secondary Structure
MKKPSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHEEMPEFVIHEYDPLIDS
CCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
SNMSPAHWQMIADDIRNNYGKYDGFVILHGTDTMAYTASALSFMLQGLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNALYIAARYPVAEVCLFFNNKLFRGNRTTKAHADGFDAFASPNFPLL
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCCCCCHHCCCCCCCEE
LEAGITINLKAGKITREATGQLEVACISPQPVGVVTLYPGISTDIFINVLQQPVKALILL
EECCEEEEEECCCEECCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
TFGVGNAPQDPALLNTLKEADERGVVLVNLTQCFQGKVNMGGYATGNALARAGVISGADM
EECCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEECCCHHHHHCCCCCCCH
TIEACLAKLHFLLSKNLPPADIKAAMQTNLVGELTSD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]