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The map label for this gene is fumB [H]

Identifier: 119774824

GI number: 119774824

Start: 2062237

End: 2063772

Strand: Direct

Name: fumB [H]

Synonym: Sama_1688

Alternate gene names: 119774824

Gene position: 2062237-2063772 (Clockwise)

Preceding gene: 119774821

Following gene: 119774825

Centisome position: 47.89

GC content: 57.03

Gene sequence:

>1536_bases
GTGTCAGAGAATCACTCGCCGGTCGTGATCAAGCAGGCCGATTTTATTGAAAGTGTTGCGGATGCACTGCAATACATCTC
CTACTACCACCCTAAAGATTTTATCGATGCCATGGCTGAGGCCTACGAGCGTGAACAGAGCGCCGCCGCCAAAGACGCTA
TCGCTCAAATTCTGATTAACTCCCGCATGTCGGCCGAGGGCAAGCGTCCTCTGTGCCAGGACACCGGCATCGTGACCTGC
TTTGTGAAAGTGGGCATGGGTGTACAGTGGGACAAAACCGACATGACAGTGCAGCAGATGGTGGATGAAGGCGTTCGCCG
CGCTTACACCAACCCGGATAACCCGCTGCGCGCCTCCATCGTTGCCGACCCTGCGGGCGCCCGTAAAAACACCAAGGACA
ACACGCCTTCCGTGGTGCATATCGACATGGTGCCAGGTAACCATGTGGAAGTGGCCATTGCGGCCAAGGGCGGCGGCAGC
GAGAACAAATCCAAGATGGTGATGCTGAACCCATCCGACGATATCGCCGCCTGGGTTGAAAAGACGCTGCCAACCATGGG
CGCTGGCTGGTGTCCTCCCGGTATGCTGGGCATAGGTATTGGTGGTACCGCCGAGAAAGCCGCCGTGATGGCTAAAGAAG
CCCTGATGGAAAGCATTGATATCCATGAGCTTATGGCCAAAGGCGCTGAAACTGCAGAAGAAAAACTGCGTCTGGATATT
TTTGAGCGTGCCAACGGTCTGGGGATTGGTGCCCAGGGCCTTGGCGGTTTGACCACAGTACTGGATGTGAAAATCAAATC
CGCGCCTACCCACGCCGCCTCCAAGCCAGTAGTGATGATCCCCAACTGCGCCGCGACCCGTCACGTGCACTTCCACCTGG
ATGGTACAGGTCCTGCCGACCTGTCTCCGCCAAGCCTCAGTGACTGGCCGGAAATTACCTGGGAAGTGGGTGAGAGCGTG
CGCCGCGTCAACCTGGACACAGTGACTCAGGCTGACATCGAAACCTGGAAGAGCGGCGATACCCTGCTGCTGTCCGGTAA
GATGCTCACCGGCCGTGACGCAGCCCACAAGCGTATTCAAACCCTGATTGAATCAGGCGAAGGCCTGCCGGAAGGTGTGG
ATTTCACCGGCAAGTTCATCTACTACGTGGGCCCGGTTGATCCGGTAGGCGATGAAGTGGTGGGTCCTGCGGGCCCAACC
ACGGCAACCCGCATGGATAAATTTACCGATCTGATGTTGGATAAGACCGGCCTGATGGGCATGATTGGTAAGGCCGAACG
GGGTCCTGCCACCGTAGAGTCTATCAAGCAGCACAAGGCGGTTTACCTGATGGCCGTTGGCGGCGCAGCCTACCTGGTGT
CCAAAGCCATTAAACACGCCCGTGTGGTGGCCTTTGAAGATTTGGGTATGGAAGCCATCTACGAGTTTGACGTGCAGGAT
ATGCCTGTGACCGTGGCAGTGGACACTCAGGGTGTAAACGCCCACGAGACAGGTCCGGCCATCTGGAAAGTGAACATCCA
GAACGCCAAGGCGTAA

Upstream 100 bases:

>100_bases
CCGAAAAAGGATTAACCAAAACAACAGGCCTCGTCCCACGGGCGCTGGGTCTTCCCTATAAATAGAGCTGTCAACAAAGC
TGAGAGAGTCGGAGCATAAC

Downstream 100 bases:

>100_bases
TAGCGTTGAATTTTGATACAAATCCATAATTGAAAAGGGCCCAGACACACTTGTCCGGGCCCTTGTCTTTGGATTAAGGT
GTCTTTTTTGCCGGGATGGC

Product: fumarate hydratase, class I, anaerobic

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSENHSPVVIKQADFIESVADALQYISYYHPKDFIDAMAEAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGIVTC
FVKVGMGVQWDKTDMTVQQMVDEGVRRAYTNPDNPLRASIVADPAGARKNTKDNTPSVVHIDMVPGNHVEVAIAAKGGGS
ENKSKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEALMESIDIHELMAKGAETAEEKLRLDI
FERANGLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPADLSPPSLSDWPEITWEVGESV
RRVNLDTVTQADIETWKSGDTLLLSGKMLTGRDAAHKRIQTLIESGEGLPEGVDFTGKFIYYVGPVDPVGDEVVGPAGPT
TATRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKQHKAVYLMAVGGAAYLVSKAIKHARVVAFEDLGMEAIYEFDVQD
MPVTVAVDTQGVNAHETGPAIWKVNIQNAKA

Sequences:

>Translated_511_residues
MSENHSPVVIKQADFIESVADALQYISYYHPKDFIDAMAEAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGIVTC
FVKVGMGVQWDKTDMTVQQMVDEGVRRAYTNPDNPLRASIVADPAGARKNTKDNTPSVVHIDMVPGNHVEVAIAAKGGGS
ENKSKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEALMESIDIHELMAKGAETAEEKLRLDI
FERANGLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPADLSPPSLSDWPEITWEVGESV
RRVNLDTVTQADIETWKSGDTLLLSGKMLTGRDAAHKRIQTLIESGEGLPEGVDFTGKFIYYVGPVDPVGDEVVGPAGPT
TATRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKQHKAVYLMAVGGAAYLVSKAIKHARVVAFEDLGMEAIYEFDVQD
MPVTVAVDTQGVNAHETGPAIWKVNIQNAKA
>Mature_510_residues
SENHSPVVIKQADFIESVADALQYISYYHPKDFIDAMAEAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGIVTCF
VKVGMGVQWDKTDMTVQQMVDEGVRRAYTNPDNPLRASIVADPAGARKNTKDNTPSVVHIDMVPGNHVEVAIAAKGGGSE
NKSKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEALMESIDIHELMAKGAETAEEKLRLDIF
ERANGLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPADLSPPSLSDWPEITWEVGESVR
RVNLDTVTQADIETWKSGDTLLLSGKMLTGRDAAHKRIQTLIESGEGLPEGVDFTGKFIYYVGPVDPVGDEVVGPAGPTT
ATRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKQHKAVYLMAVGGAAYLVSKAIKHARVVAFEDLGMEAIYEFDVQDM
PVTVAVDTQGVNAHETGPAIWKVNIQNAKA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=473, Percent_Identity=32.5581395348837, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1787897, Length=472, Percent_Identity=33.2627118644068, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1789442, Length=295, Percent_Identity=29.8305084745763, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=31.8435754189944, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54688; Mature: 54557

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENHSPVVIKQADFIESVADALQYISYYHPKDFIDAMAEAYEREQSAAAKDAIAQILIN
CCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRMSAEGKRPLCQDTGIVTCFVKVGMGVQWDKTDMTVQQMVDEGVRRAYTNPDNPLRASI
CCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
VADPAGARKNTKDNTPSVVHIDMVPGNHVEVAIAAKGGGSENKSKMVMLNPSDDIAAWVE
EECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHH
KTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEALMESIDIHELMAKGAETAEEKLRLDI
HHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHH
FERANGLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPAD
HHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCC
LSPPSLSDWPEITWEVGESVRRVNLDTVTQADIETWKSGDTLLLSGKMLTGRDAAHKRIQ
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCEECCCHHHHHHHH
TLIESGEGLPEGVDFTGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTDLMLDKTGLMG
HHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
MIGKAERGPATVESIKQHKAVYLMAVGGAAYLVSKAIKHARVVAFEDLGMEAIYEFDVQD
HCCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHEEEEEHHCCCCEEEECCCCC
MPVTVAVDTQGVNAHETGPAIWKVNIQNAKA
CCEEEEEECCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
SENHSPVVIKQADFIESVADALQYISYYHPKDFIDAMAEAYEREQSAAAKDAIAQILIN
CCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRMSAEGKRPLCQDTGIVTCFVKVGMGVQWDKTDMTVQQMVDEGVRRAYTNPDNPLRASI
CCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
VADPAGARKNTKDNTPSVVHIDMVPGNHVEVAIAAKGGGSENKSKMVMLNPSDDIAAWVE
EECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHH
KTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEALMESIDIHELMAKGAETAEEKLRLDI
HHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHH
FERANGLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPAD
HHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCC
LSPPSLSDWPEITWEVGESVRRVNLDTVTQADIETWKSGDTLLLSGKMLTGRDAAHKRIQ
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCEECCCHHHHHHHH
TLIESGEGLPEGVDFTGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTDLMLDKTGLMG
HHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
MIGKAERGPATVESIKQHKAVYLMAVGGAAYLVSKAIKHARVVAFEDLGMEAIYEFDVQD
HCCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHEEEEEHHCCCCEEEECCCCC
MPVTVAVDTQGVNAHETGPAIWKVNIQNAKA
CCEEEEEECCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]