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The map label for this gene is hydA [H]

Identifier: 119774815

GI number: 119774815

Start: 2053436

End: 2054572

Strand: Reverse

Name: hydA [H]

Synonym: Sama_1679

Alternate gene names: 119774815

Gene position: 2054572-2053436 (Counterclockwise)

Preceding gene: 119774820

Following gene: 119774814

Centisome position: 47.71

GC content: 57.52

Gene sequence:

>1137_bases
ATGGATACACATGCAGCCCTCTACGAGCAAGGAAAGCAGCGACTTGAAACGCTGCGTTCCCTGCCAAGGCGCCATGAGGA
GACCATCTTTGACAAGATGGCTCAACATGGCATTACCCGTCGTGATTTTATGAAGTGGAGCGCCTCGGTTACCGCCATGC
TGGCGCTGCCGTTGCCCTTCTCTACCCTGGTGGCCGAAGCGGCCGAACTGGCAGACCGCGTGCCACTGATTTGGCTGCAC
ATGGCCGAATGTACCGGCTGCTCTGAGTCGCTGGTACGCGCCGACACGCCAAATCTCGACTCCCTAATCTTCGACCATAT
CAGCCTCGAATACCACGAGACTCTGATGGCCGCTGCCGGTTGGCAAGCCGAGGAAAACCTCGAACACGCCCTGGAAACCT
ACAAAGGCAATTATCTGCTGGCAGTGGAAGGCGCCGTCCCCACCGCCAATAACGGTGCTTTCCTGACCGTGGGTTGTAAA
GGTCACACCGGGCTTGAAATCATCAAGCATGCGGCCGATGGCGCGGCAGCCATTGTATCCGTGGGTACCTGCGCCTCCTT
CGGCGGCGTTCAGGCTGCCTACCCCAACCCCACCGGGGCCAAAGGGGTACACGAAGTTGTCAGCAAGCCTGTGATTAACC
TGGGCGGCTGTCCACCGAGTGAGAAAAATATTGTTGGCACCCTGATGTATTTCATCATGTTCGGCAAGCTGCCGGCACTG
GATATGTTTAATCGTCCCAAGTGGGCCTATGGCGCCCGGGTACACGATAACTGTGAACGTCGTGGCCGCTTTGATGCCGG
TGAGTTTGTTGAAGAGTTTGGCGATCACGGCGCCAAAGAAGGCTACTGCCTCTACAAGGTTGGCTGTAAAGGCCCTTACA
CCTACAACAACTGCCCCACAGAACGCTTCAATCATCATACCAGCTGGCCGGTACTCGCGGGCCACGGCTGTATGGGCTGC
TCTGAGCCAAACTTCTGGGACGACATGGCCGACTTTGAGAAGCCACTTGGCCGGCAACTGCTGCACGGGCTTGATGCCAC
GGCCGACACCATAGGCGCCGTCATTCTCGGTGCCACTGTTGTCGGTATCGGCGCCCATGCCGTTGCCAGTGTATTTGCGC
AAGCCAAAGAGGACTAA

Upstream 100 bases:

>100_bases
GTATCAATATGATGACGAAGCCCCCTTCCTACACTTTTCCTGAAATGTCGTGCATGCCAAAAGCATGTTTACAACTTAAA
AACAAAAAAAGGTCGTCACC

Downstream 100 bases:

>100_bases
ATCATGAGCCAACGTGTTGTTATCGATCCCATCACCCGTATCGAGGGCCATTTGCGCGTCGAGGTCGAGGTAGATGAGAA
CAATGTGATTCAGAACGCCT

Product: hydrogen:quinone oxidoreductase

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MDTHAALYEQGKQRLETLRSLPRRHEETIFDKMAQHGITRRDFMKWSASVTAMLALPLPFSTLVAEAAELADRVPLIWLH
MAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAGWQAEENLEHALETYKGNYLLAVEGAVPTANNGAFLTVGCK
GHTGLEIIKHAADGAAAIVSVGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCMGC
SEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATVVGIGAHAVASVFAQAKED

Sequences:

>Translated_378_residues
MDTHAALYEQGKQRLETLRSLPRRHEETIFDKMAQHGITRRDFMKWSASVTAMLALPLPFSTLVAEAAELADRVPLIWLH
MAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAGWQAEENLEHALETYKGNYLLAVEGAVPTANNGAFLTVGCK
GHTGLEIIKHAADGAAAIVSVGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCMGC
SEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATVVGIGAHAVASVFAQAKED
>Mature_378_residues
MDTHAALYEQGKQRLETLRSLPRRHEETIFDKMAQHGITRRDFMKWSASVTAMLALPLPFSTLVAEAAELADRVPLIWLH
MAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAGWQAEENLEHALETYKGNYLLAVEGAVPTANNGAFLTVGCK
GHTGLEIIKHAADGAAAIVSVGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCMGC
SEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATVVGIGAHAVASVFAQAKED

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=334, Percent_Identity=44.311377245509, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1789371, Length=289, Percent_Identity=49.8269896193772, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 40997; Mature: 40997

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTHAALYEQGKQRLETLRSLPRRHEETIFDKMAQHGITRRDFMKWSASVTAMLALPLPF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCH
STLVAEAAELADRVPLIWLHMAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAG
HHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLEHALETYKGNYLLAVEGAVPTANNGAFLTVGCKGHTGLEIIKHAADGAAAIVS
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHH
VGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
HHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCMGCSEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATV
HHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VGIGAHAVASVFAQAKED
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTHAALYEQGKQRLETLRSLPRRHEETIFDKMAQHGITRRDFMKWSASVTAMLALPLPF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCH
STLVAEAAELADRVPLIWLHMAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAG
HHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLEHALETYKGNYLLAVEGAVPTANNGAFLTVGCKGHTGLEIIKHAADGAAAIVS
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHH
VGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
HHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCMGCSEPNFWDDMADFEKPLGRQLLHGLDATADTIGAVILGATV
HHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VGIGAHAVASVFAQAKED
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]