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The map label for this gene is dapE

Identifier: 119774783

GI number: 119774783

Start: 2012345

End: 2013493

Strand: Reverse

Name: dapE

Synonym: Sama_1647

Alternate gene names: 119774783

Gene position: 2013493-2012345 (Counterclockwise)

Preceding gene: 119774784

Following gene: 119774782

Centisome position: 46.76

GC content: 57.96

Gene sequence:

>1149_bases
ATGAGTAGCCCAGAAACCCACAGTGATGTGATTGAACTGACGCGCGAGCTCATTTCACGCCCGTCTGTCACCCCGCTTGA
TGAAGGCTGTCAGGACCTGATGGCCAAACGTCTCGCCGCCATTGGCTTTACCATCGAGCCCATGGTATTTGAAGACACCA
CCAACCTCTGGGCCCGCCGTGGCACTGAGGGTCCGGTATTCTGTTTTGCAGGCCACACTGATGTGGTGCCGGTTGGCGAT
CTGAATCGGTGGCACACCCCGCCATTCGATCCTGTGGTGATTGACGGTTATATCCACGGCCGCGGCGCCGCCGACATGAA
AGGCTCACTCGCCGCCATGGTGGTGGCCGCTGAGCGATTTGTGGCCGAGCACCCGGACCATCAGGGCTCCATCGCCTTTC
TTATCACCTCCGATGAAGAAGGCCCCTTTATTAATGGCACTGTGCGTGTTGTTGAAACGCTTGAAGCACGCCATGAAAAG
ATAACCTGGGCCTTGGTGGGCGAGCCTTCCAGCACCCATCTTCTCGGGGATGTGGTGAAAAACGGCCGTCGGGGCAGCCT
TACTGGCAACCTCACAGTCAAAGGTATTCAGGGCCATGTGGCCTACCCCCATTTGGCCGATAACCCAATCCACAGGGCGG
CACCGGCCCTGGCAGAGTTATCCCGTATCGAGTGGGACAAGGGCAATGAATTCTTCCCGCCCACCAGTTTTCAGATTGCC
AACATCAACGGCGGCACGGGCGCATCCAATGTGATCCCGGGTGAACTCAAGGTGATGTTCAACTTCCGTTACTCCACCGA
AGTGACCGCTGAGACCCTGATTGCCCGCGTACTGGGTATTCTCGATGCCCATGGGCTCGATTACGACATCGACTGGGTGT
TTAACGGCCTGCCTTTCCTCACCGGAGAAGGCCCACTGCTGGATGCCACCCGGGAAGCAATTTTCGAGGTAACAGGTACC
CACACCGACCCGCAAACCACCGGCGGCACCTCCGATGGCCGCTTTATCGCGCCCACCGGTGCTCAGGTGATTGAGCTCGG
TCCGGTCAATGCCACCATTCACAAGGTGAACGAGTGCGTGAAGGCAAGCGATCTTGAGCTGCTCACCGGCTGCTACCAGC
GCATTCTGGAAAAGCTGCTGTGCAACTGA

Upstream 100 bases:

>100_bases
TGCTGGAGTACCCCACACTCATCAAGAGGCCGGTGCTGGTAAAAGGCGACACCGTGTACACAGGCTTTAACGACAAGCTG
TACAGCGAGGTATTTGGCCA

Downstream 100 bases:

>100_bases
GCTGGATTTACGGTGCCGCCGAGCCACCGCTCGTGAGCCATGACGGGGTGATGATGCACCCCGCCGCCGCAAAGGCCTTT
GCGGCCATGCAGCATCAGGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSSPETHSDVIELTRELISRPSVTPLDEGCQDLMAKRLAAIGFTIEPMVFEDTTNLWARRGTEGPVFCFAGHTDVVPVGD
LNRWHTPPFDPVVIDGYIHGRGAADMKGSLAAMVVAAERFVAEHPDHQGSIAFLITSDEEGPFINGTVRVVETLEARHEK
ITWALVGEPSSTHLLGDVVKNGRRGSLTGNLTVKGIQGHVAYPHLADNPIHRAAPALAELSRIEWDKGNEFFPPTSFQIA
NINGGTGASNVIPGELKVMFNFRYSTEVTAETLIARVLGILDAHGLDYDIDWVFNGLPFLTGEGPLLDATREAIFEVTGT
HTDPQTTGGTSDGRFIAPTGAQVIELGPVNATIHKVNECVKASDLELLTGCYQRILEKLLCN

Sequences:

>Translated_382_residues
MSSPETHSDVIELTRELISRPSVTPLDEGCQDLMAKRLAAIGFTIEPMVFEDTTNLWARRGTEGPVFCFAGHTDVVPVGD
LNRWHTPPFDPVVIDGYIHGRGAADMKGSLAAMVVAAERFVAEHPDHQGSIAFLITSDEEGPFINGTVRVVETLEARHEK
ITWALVGEPSSTHLLGDVVKNGRRGSLTGNLTVKGIQGHVAYPHLADNPIHRAAPALAELSRIEWDKGNEFFPPTSFQIA
NINGGTGASNVIPGELKVMFNFRYSTEVTAETLIARVLGILDAHGLDYDIDWVFNGLPFLTGEGPLLDATREAIFEVTGT
HTDPQTTGGTSDGRFIAPTGAQVIELGPVNATIHKVNECVKASDLELLTGCYQRILEKLLCN
>Mature_381_residues
SSPETHSDVIELTRELISRPSVTPLDEGCQDLMAKRLAAIGFTIEPMVFEDTTNLWARRGTEGPVFCFAGHTDVVPVGDL
NRWHTPPFDPVVIDGYIHGRGAADMKGSLAAMVVAAERFVAEHPDHQGSIAFLITSDEEGPFINGTVRVVETLEARHEKI
TWALVGEPSSTHLLGDVVKNGRRGSLTGNLTVKGIQGHVAYPHLADNPIHRAAPALAELSRIEWDKGNEFFPPTSFQIAN
INGGTGASNVIPGELKVMFNFRYSTEVTAETLIARVLGILDAHGLDYDIDWVFNGLPFLTGEGPLLDATREAIFEVTGTH
TDPQTTGGTSDGRFIAPTGAQVIELGPVNATIHKVNECVKASDLELLTGCYQRILEKLLCN

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=381, Percent_Identity=64.5669291338583, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1789236, Length=253, Percent_Identity=23.7154150197628, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790395, Length=269, Percent_Identity=27.1375464684015, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17551016, Length=285, Percent_Identity=28.0701754385965, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_SHEAM (A1S647)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927523.1
- ProteinModelPortal:   A1S647
- SMR:   A1S647
- STRING:   A1S647
- MEROPS:   M20.010
- GeneID:   4603898
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1647
- NMPDR:   fig|326297.7.peg.1556
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   A1S647
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41367; Mature: 41236

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 74-74 ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPETHSDVIELTRELISRPSVTPLDEGCQDLMAKRLAAIGFTIEPMVFEDTTNLWARR
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEECCEEEECCHHHHHHC
GTEGPVFCFAGHTDVVPVGDLNRWHTPPFDPVVIDGYIHGRGAADMKGSLAAMVVAAERF
CCCCCEEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHH
VAEHPDHQGSIAFLITSDEEGPFINGTVRVVETLEARHEKITWALVGEPSSTHLLGDVVK
HHHCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHH
NGRRGSLTGNLTVKGIQGHVAYPHLADNPIHRAAPALAELSRIEWDKGNEFFPPTSFQIA
CCCCCCEEECEEEEEEECCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
NINGGTGASNVIPGELKVMFNFRYSTEVTAETLIARVLGILDAHGLDYDIDWVFNGLPFL
ECCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEHHEEECCCCEE
TGEGPLLDATREAIFEVTGTHTDPQTTGGTSDGRFIAPTGAQVIELGPVNATIHKVNECV
CCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHH
KASDLELLTGCYQRILEKLLCN
HHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSPETHSDVIELTRELISRPSVTPLDEGCQDLMAKRLAAIGFTIEPMVFEDTTNLWARR
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEECCEEEECCHHHHHHC
GTEGPVFCFAGHTDVVPVGDLNRWHTPPFDPVVIDGYIHGRGAADMKGSLAAMVVAAERF
CCCCCEEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHH
VAEHPDHQGSIAFLITSDEEGPFINGTVRVVETLEARHEKITWALVGEPSSTHLLGDVVK
HHHCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHH
NGRRGSLTGNLTVKGIQGHVAYPHLADNPIHRAAPALAELSRIEWDKGNEFFPPTSFQIA
CCCCCCEEECEEEEEEECCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
NINGGTGASNVIPGELKVMFNFRYSTEVTAETLIARVLGILDAHGLDYDIDWVFNGLPFL
ECCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEHHEEECCCCEE
TGEGPLLDATREAIFEVTGTHTDPQTTGGTSDGRFIAPTGAQVIELGPVNATIHKVNECV
CCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHH
KASDLELLTGCYQRILEKLLCN
HHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA