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The map label for this gene is pleD [H]

Identifier: 119774691

GI number: 119774691

Start: 1891393

End: 1893582

Strand: Reverse

Name: pleD [H]

Synonym: Sama_1554

Alternate gene names: 119774691

Gene position: 1893582-1891393 (Counterclockwise)

Preceding gene: 119774692

Following gene: 119774688

Centisome position: 43.97

GC content: 51.32

Gene sequence:

>2190_bases
TTGTCATATTTTTTTTCAGCATCGCCGCCACGCACTGCGATCATATTGGCTTTTTGCGGCTTCTTACTGAACCTATATCC
GCTGCCCCTGTTTGCCAATGTACAGCTGATTTTGGGTAATGCTGCCTATGTGATTGCCGCGGTAGTATTGGGACCGCGCT
ATGCCGTCGCCGTGGCTCTGTCCTGCGCAGCAGGCTTGTTTATGATTTGGGGGACTACACATGTATTTGTCCTCTTCCCC
CTCGAGGCCCTGGTGTTGGCCATGGCCACGAGACGCGGTATTTACTCCCTTTATGCCGGTGTGGGTTTCTGGCTGCTGAT
TGGTACCCCACTGTTTTACCTTTACGGCCTCACGTTAACTTCGTTGCCTGTAAGTCACCTGCCCTTCATCGCCTTTAAAC
AAGCAATCAACGGTTTGTTTTACATTGGTTTGGGCGCCGTGTTGCTGTTGATGTTACCTAAACTGACGCTGAGCGGTATC
AGTCATCCGAAGACTCCAGAACGTTTCAGCGATAAGTTAACCTACAGCTTTACTCTGTTTATTTCTCTGGCAATGCTGAT
CGCCGCCCTGTTGTTCAATCAGTTTTTTATTGAGCGCCAGCAGCTGCTGATCAAAAAGAACCTCCAGAACAATGCGTTAC
ATTTGTCGCAATTCACCTTCGATTACCTGCAGGAGCATCAGAGGGCCATCGCTCAGGGCGCCCAATGGCTGTCGATGATG
GATGGCGGCTCGGAGAAACAGCAACATTGGCTCACTCTATTCAACCATGGTTACCCTGCTTTTTTAACCATGTTGATTGC
CAATGAACGAGGCCATATCGTGGCCGCGAGCCCAGCCGCACGCATGATGGATGAAGCGATTAAAAAAGGTGAATACAGCG
TGGCCGACCGCCACTACTTTAAGGAAGCCTTTTATAACCAGACTCCCTTTGTTTCGCCGGTATTTATGGGCCGGGGTTTT
GGCAGCGACCCCATAGTCGCTGTCAGTGCCCCAATATACAGCCAAGGCCGTCCGAGCCATCCGTCAGGCATTCTGGAAGG
CTCCCTTGATTTAAATCGATTTGTGCTTATCGACAAGCAAAACCGCCATCATTCAGAACAATACCTGCTGCTCACCGACG
AAAACAACCGGGTGATTTATGCATCGGCGCCACTGAATATTACGGCACTGAGCGAGTTCGCGTCCGCCGAGAGTGGCCTT
GAGTACCGCACCAGTTTGCAGTTGATTAACCTGCATGATTTAACCAATCCCAACCCCGAGTATATCTATGCTCAGGAGCC
ATTGGCGAATGGTTGGCGCCTCATGGTCTTAACGCCCTTCGCACCACTGATGCAAATGGCCGAAACACAGTTTTTACGCA
CCTGTATACTGCTCATTATCAGCATGGGATTCAGCTTCTATCTGGCCCGTTTGATAAGCCGGATGCTGACCAGCCCCCTT
GAAACCATTGCCCATGAATTCAATAGTCCCGGTAAGCAATCCAAGCCACTGCCCGCCGATGCCCCGGCCGAAGTACAGAC
GCTCTATCAGAGCCTGAAAGCGAGTCAGCAGCAATTGCTGAGCTATCAGTTGGAGCTGGAAGAAAAGGTTGCCTTTCGCA
CCTTTGAACTTGAAAAAGCCAATCAGAAACTGCAGGCACTTGCCGAGCGCGACCCCCTGACGGGTTTATATAACCGGCGC
TATACAACGGCAGGATTTGCACCTTTACAAGCCATGTGTGAGCGCAGTGGTGAAGCCATCGCGGTGGTGATCCTGGATCT
CGATTTTTTTAAAGCCATCAATGATAATCACGGGCATCTGGCCGGGGATGAGTGTCTGCGTCAGGTAGCCATTCTGCTTA
CACAACACTTTAAACGGGATTCTGATCTGGTTGCCCGTTATGGCGGAGAAGAATTTTTACTGATCCTGCCAATGACCAAT
GCACTGAAGATTGAGCATCACCTCAACCTGCTGCGGGAGCAGTTGGCAAGGCATCCTGTGGTTCTGCCCGGCGCCCAAGA
GCCCATTCACCTGTCTGTTTCGGTCGGCGCCGCGGTGGCCAATGCCAGCTTCTCCGACACTCTTGAAGCCTGGCTCAAAG
TCGCCGATGATAACCTCTATCAGGCTAAAGCCGAGGGCCGTAATCGGGTGGTCTGTACCCTGATTAATGACGAAATACCG
AGACAACAGGTCATGGACCATAAGCCATAG

Upstream 100 bases:

>100_bases
TAACCTCACTTTCATAGGTTTACGCTGCCAATGATTGTGAACAAAGCGTTAACACTCTAAAGTAAAGGCGATAATACAGG
CGGATTTTGGAGAATCATTC

Downstream 100 bases:

>100_bases
ACCTGCTGATAACTAAAAGGCCACAGCGTCATGTGCTGTGGCCTTGTTACTGAACGTCCCAACTTATCCCGGTGATGTGG
TCTTTCACAATCTCGCTGCT

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MSYFFSASPPRTAIILAFCGFLLNLYPLPLFANVQLILGNAAYVIAAVVLGPRYAVAVALSCAAGLFMIWGTTHVFVLFP
LEALVLAMATRRGIYSLYAGVGFWLLIGTPLFYLYGLTLTSLPVSHLPFIAFKQAINGLFYIGLGAVLLLMLPKLTLSGI
SHPKTPERFSDKLTYSFTLFISLAMLIAALLFNQFFIERQQLLIKKNLQNNALHLSQFTFDYLQEHQRAIAQGAQWLSMM
DGGSEKQQHWLTLFNHGYPAFLTMLIANERGHIVAASPAARMMDEAIKKGEYSVADRHYFKEAFYNQTPFVSPVFMGRGF
GSDPIVAVSAPIYSQGRPSHPSGILEGSLDLNRFVLIDKQNRHHSEQYLLLTDENNRVIYASAPLNITALSEFASAESGL
EYRTSLQLINLHDLTNPNPEYIYAQEPLANGWRLMVLTPFAPLMQMAETQFLRTCILLIISMGFSFYLARLISRMLTSPL
ETIAHEFNSPGKQSKPLPADAPAEVQTLYQSLKASQQQLLSYQLELEEKVAFRTFELEKANQKLQALAERDPLTGLYNRR
YTTAGFAPLQAMCERSGEAIAVVILDLDFFKAINDNHGHLAGDECLRQVAILLTQHFKRDSDLVARYGGEEFLLILPMTN
ALKIEHHLNLLREQLARHPVVLPGAQEPIHLSVSVGAAVANASFSDTLEAWLKVADDNLYQAKAEGRNRVVCTLINDEIP
RQQVMDHKP

Sequences:

>Translated_729_residues
MSYFFSASPPRTAIILAFCGFLLNLYPLPLFANVQLILGNAAYVIAAVVLGPRYAVAVALSCAAGLFMIWGTTHVFVLFP
LEALVLAMATRRGIYSLYAGVGFWLLIGTPLFYLYGLTLTSLPVSHLPFIAFKQAINGLFYIGLGAVLLLMLPKLTLSGI
SHPKTPERFSDKLTYSFTLFISLAMLIAALLFNQFFIERQQLLIKKNLQNNALHLSQFTFDYLQEHQRAIAQGAQWLSMM
DGGSEKQQHWLTLFNHGYPAFLTMLIANERGHIVAASPAARMMDEAIKKGEYSVADRHYFKEAFYNQTPFVSPVFMGRGF
GSDPIVAVSAPIYSQGRPSHPSGILEGSLDLNRFVLIDKQNRHHSEQYLLLTDENNRVIYASAPLNITALSEFASAESGL
EYRTSLQLINLHDLTNPNPEYIYAQEPLANGWRLMVLTPFAPLMQMAETQFLRTCILLIISMGFSFYLARLISRMLTSPL
ETIAHEFNSPGKQSKPLPADAPAEVQTLYQSLKASQQQLLSYQLELEEKVAFRTFELEKANQKLQALAERDPLTGLYNRR
YTTAGFAPLQAMCERSGEAIAVVILDLDFFKAINDNHGHLAGDECLRQVAILLTQHFKRDSDLVARYGGEEFLLILPMTN
ALKIEHHLNLLREQLARHPVVLPGAQEPIHLSVSVGAAVANASFSDTLEAWLKVADDNLYQAKAEGRNRVVCTLINDEIP
RQQVMDHKP
>Mature_728_residues
SYFFSASPPRTAIILAFCGFLLNLYPLPLFANVQLILGNAAYVIAAVVLGPRYAVAVALSCAAGLFMIWGTTHVFVLFPL
EALVLAMATRRGIYSLYAGVGFWLLIGTPLFYLYGLTLTSLPVSHLPFIAFKQAINGLFYIGLGAVLLLMLPKLTLSGIS
HPKTPERFSDKLTYSFTLFISLAMLIAALLFNQFFIERQQLLIKKNLQNNALHLSQFTFDYLQEHQRAIAQGAQWLSMMD
GGSEKQQHWLTLFNHGYPAFLTMLIANERGHIVAASPAARMMDEAIKKGEYSVADRHYFKEAFYNQTPFVSPVFMGRGFG
SDPIVAVSAPIYSQGRPSHPSGILEGSLDLNRFVLIDKQNRHHSEQYLLLTDENNRVIYASAPLNITALSEFASAESGLE
YRTSLQLINLHDLTNPNPEYIYAQEPLANGWRLMVLTPFAPLMQMAETQFLRTCILLIISMGFSFYLARLISRMLTSPLE
TIAHEFNSPGKQSKPLPADAPAEVQTLYQSLKASQQQLLSYQLELEEKVAFRTFELEKANQKLQALAERDPLTGLYNRRY
TTAGFAPLQAMCERSGEAIAVVILDLDFFKAINDNHGHLAGDECLRQVAILLTQHFKRDSDLVARYGGEEFLLILPMTNA
LKIEHHLNLLREQLARHPVVLPGAQEPIHLSVSVGAAVANASFSDTLEAWLKVADDNLYQAKAEGRNRVVCTLINDEIPR
QQVMDHKP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=185, Percent_Identity=37.2972972972973, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=38.125, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=32.9341317365269, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=174, Percent_Identity=36.7816091954023, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1787802, Length=196, Percent_Identity=29.0816326530612, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI87081881, Length=179, Percent_Identity=35.7541899441341, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=32.3529411764706, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=34.8837209302326, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788085, Length=200, Percent_Identity=27.5, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788956, Length=264, Percent_Identity=28.030303030303, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=142, Percent_Identity=33.8028169014084, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=31.4606741573034, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=32.0754716981132, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI87081977, Length=179, Percent_Identity=30.7262569832402, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 81451; Mature: 81320

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYFFSASPPRTAIILAFCGFLLNLYPLPLFANVQLILGNAAYVIAAVVLGPRYAVAVAL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCEEEEECCHHHHHHHHHHCCHHHHHHHH
SCAAGLFMIWGTTHVFVLFPLEALVLAMATRRGIYSLYAGVGFWLLIGTPLFYLYGLTLT
HHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLPVSHLPFIAFKQAINGLFYIGLGAVLLLMLPKLTLSGISHPKTPERFSDKLTYSFTLF
CCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHH
ISLAMLIAALLFNQFFIERQQLLIKKNLQNNALHLSQFTFDYLQEHQRAIAQGAQWLSMM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
DGGSEKQQHWLTLFNHGYPAFLTMLIANERGHIVAASPAARMMDEAIKKGEYSVADRHYF
CCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHH
KEAFYNQTPFVSPVFMGRGFGSDPIVAVSAPIYSQGRPSHPSGILEGSLDLNRFVLIDKQ
HHHHCCCCCCCCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEECCCCCCEEEEEECC
NRHHSEQYLLLTDENNRVIYASAPLNITALSEFASAESGLEYRTSLQLINLHDLTNPNPE
CCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCEEHHEEEEEEECCCCCCCC
YIYAQEPLANGWRLMVLTPFAPLMQMAETQFLRTCILLIISMGFSFYLARLISRMLTSPL
EEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETIAHEFNSPGKQSKPLPADAPAEVQTLYQSLKASQQQLLSYQLELEEKVAFRTFELEKA
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHH
NQKLQALAERDPLTGLYNRRYTTAGFAPLQAMCERSGEAIAVVILDLDFFKAINDNHGHL
HHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHCCCCEEEEEEEEHHHHHHHCCCCCCC
AGDECLRQVAILLTQHFKRDSDLVARYGGEEFLLILPMTNALKIEHHLNLLREQLARHPV
CHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCE
VLPGAQEPIHLSVSVGAAVANASFSDTLEAWLKVADDNLYQAKAEGRNRVVCTLINDEIP
ECCCCCCCEEEEEEECHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCCC
RQQVMDHKP
HHHHCCCCC
>Mature Secondary Structure 
SYFFSASPPRTAIILAFCGFLLNLYPLPLFANVQLILGNAAYVIAAVVLGPRYAVAVAL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCEEEEECCHHHHHHHHHHCCHHHHHHHH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HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA