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The map label for this gene is lasB [H]

Identifier: 119774338

GI number: 119774338

Start: 1473553

End: 1475010

Strand: Direct

Name: lasB [H]

Synonym: Sama_1201

Alternate gene names: 119774338

Gene position: 1473553-1475010 (Clockwise)

Preceding gene: 119774336

Following gene: 119774351

Centisome position: 34.22

GC content: 54.18

Gene sequence:

>1458_bases
ATGAAAGCATACACTTTAATCCCTGCAACACTGGCTCTGTGTGTTTCCGGCGCACTGCAGGCTGGTAATACCCTGGAACA
CCGCATGGGCCTGGCCCATGGCAACAGCTTTACTGCCGGTAAATCTTTCACTACTGCTGCTGGTCACCAAAAAGTTAAGC
AACAGCAACTGTTCAATGGTGTAAAGGTGTACGGCCACCACCTGGTCGTGTCCTCTGATGGTGATATTTTTGGTCAGGCC
GCCGCTATTGAGGCCGACTTTAACGTTACCCCAGCCTTGACCCGTGGCCAGGCGATTGCTGCGCTGAACAAAGTGTATCC
CGGCGCCGTGGCATCAGGCCATAAAGACGTAGAGCTGGTGATTCTGGCGGAAGGGGATGCCCCACGTCTGGCTTACCGCG
TGTCTTTCCTCAGCGAAGGTGTAAACGAGATTTCCCGTCCCGCCGGTTTGGTGGATGCCATGACCGGTGAGGTGGTGCGT
CACTGGAACGAGTTGATGACCGCCAAAAACAGCGCAGGCAAGCCGGGCAACGGCGGTGGCACGACTCCAGGCACTGCATT
TGATGCCACAGGTCCGGGCGGCAATGCCAAAACCGGCCAGTACTACTACGGCAGTGACTTCGGCCCGCTGAACGTTGAAG
AGGCCAACGGCGTATGTACCATGAACAACGCCAACGTGAAGACAGTGGACATGGCCAACAGTACTCGCCGTGGCAGTGTT
TACTCTTTCACCTGTCCTGAAAACACCTATCGTGCCATCAATGGCGCATACTCTCCGCTGAACGATGCTCACTACTTTGG
CGGCGTGATTTTCAACATGTACAAAGACTGGTACAACACAGCCCCGCTGACCTTCCAGCTGGAAATGCGTGTTCACTATG
GCCGTAACTACGAAAACGCCTTCTGGGATGGCCGTGCCATGTCCTTCGGTGACGGCGCCAGCTATTTCTATCCTCTGGTG
AGTTTGGATGTTTCAGCTCACGAAGTGAGCCACGGCTTCACCGAACAGAACTCAGGCCTGGAATACAGTGCTCAGTCCGG
TGGTATGAACGAAGCTTTCTCCGATATGGCCGGTGAAGCTGCCGAATTCTTTATGCGTGGCAGCAACGACTGGATGGTGG
GTCATGACATCTTCAAAGGTAATGGAGCCCTGCGTTACATGGACGACCCAACCAAAGATGGTCGCTCAATTGGTCATGCC
TCAGATTACACCAGTGGTATGGACGTGCACTATTCTTCCGGTGTGTACAATAAGGCTTTCTACCTGCTGGCCAAGAAAAA
TGGCTGGGATACCCGTAAAGCCTTCGAAGTGTTTGTTCTGGCAAACCAGCATTACTGGACAGCGACCAGCAATTACAATC
AGGGCGCCTGTGGTGTAGAGGCGGCCGCTTCCGATAAGGGTTATGCAGTGTCTGATGTGACCGCCGCCTTTGCGTCGGTT
GGCGTAAGCTGCCAGTAA

Upstream 100 bases:

>100_bases
TTTAAGGTTGCATTCATTCTTGAGCGCCTGATGGATTTGTGACAGTTTGTTCCGACCAAGGTGACACTCTTGGACAAAAC
AACTACAAAAGGATCAGGAC

Downstream 100 bases:

>100_bases
GGTAAAGCGTACGAAAAAGCCCGGACAGTCCGGGCTTTTTTATTGGCGTTGTTTTGTCTGTTTGGTGCGCTTGTGGTGGT
ACATGCGGGCATCGACGCTT

Product: peptidase M4, thermolysin

Products: NA

Alternate protein names: Elastase; Neutral metalloproteinase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKAYTLIPATLALCVSGALQAGNTLEHRMGLAHGNSFTAGKSFTTAAGHQKVKQQQLFNGVKVYGHHLVVSSDGDIFGQA
AAIEADFNVTPALTRGQAIAALNKVYPGAVASGHKDVELVILAEGDAPRLAYRVSFLSEGVNEISRPAGLVDAMTGEVVR
HWNELMTAKNSAGKPGNGGGTTPGTAFDATGPGGNAKTGQYYYGSDFGPLNVEEANGVCTMNNANVKTVDMANSTRRGSV
YSFTCPENTYRAINGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQLEMRVHYGRNYENAFWDGRAMSFGDGASYFYPLV
SLDVSAHEVSHGFTEQNSGLEYSAQSGGMNEAFSDMAGEAAEFFMRGSNDWMVGHDIFKGNGALRYMDDPTKDGRSIGHA
SDYTSGMDVHYSSGVYNKAFYLLAKKNGWDTRKAFEVFVLANQHYWTATSNYNQGACGVEAAASDKGYAVSDVTAAFASV
GVSCQ

Sequences:

>Translated_485_residues
MKAYTLIPATLALCVSGALQAGNTLEHRMGLAHGNSFTAGKSFTTAAGHQKVKQQQLFNGVKVYGHHLVVSSDGDIFGQA
AAIEADFNVTPALTRGQAIAALNKVYPGAVASGHKDVELVILAEGDAPRLAYRVSFLSEGVNEISRPAGLVDAMTGEVVR
HWNELMTAKNSAGKPGNGGGTTPGTAFDATGPGGNAKTGQYYYGSDFGPLNVEEANGVCTMNNANVKTVDMANSTRRGSV
YSFTCPENTYRAINGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQLEMRVHYGRNYENAFWDGRAMSFGDGASYFYPLV
SLDVSAHEVSHGFTEQNSGLEYSAQSGGMNEAFSDMAGEAAEFFMRGSNDWMVGHDIFKGNGALRYMDDPTKDGRSIGHA
SDYTSGMDVHYSSGVYNKAFYLLAKKNGWDTRKAFEVFVLANQHYWTATSNYNQGACGVEAAASDKGYAVSDVTAAFASV
GVSCQ
>Mature_485_residues
MKAYTLIPATLALCVSGALQAGNTLEHRMGLAHGNSFTAGKSFTTAAGHQKVKQQQLFNGVKVYGHHLVVSSDGDIFGQA
AAIEADFNVTPALTRGQAIAALNKVYPGAVASGHKDVELVILAEGDAPRLAYRVSFLSEGVNEISRPAGLVDAMTGEVVR
HWNELMTAKNSAGKPGNGGGTTPGTAFDATGPGGNAKTGQYYYGSDFGPLNVEEANGVCTMNNANVKTVDMANSTRRGSV
YSFTCPENTYRAINGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQLEMRVHYGRNYENAFWDGRAMSFGDGASYFYPLV
SLDVSAHEVSHGFTEQNSGLEYSAQSGGMNEAFSDMAGEAAEFFMRGSNDWMVGHDIFKGNGALRYMDDPTKDGRSIGHA
SDYTSGMDVHYSSGVYNKAFYLLAKKNGWDTRKAFEVFVLANQHYWTATSNYNQGACGVEAAASDKGYAVSDVTAAFASV
GVSCQ

Specific function: Cleaves elastin, collagen, IgG, and several complement components. Involved in the pathogenesis of P.aeruginosa infections [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C [H]

EC number: =3.4.24.26 [H]

Molecular weight: Translated: 51974; Mature: 51974

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYTLIPATLALCVSGALQAGNTLEHRMGLAHGNSFTAGKSFTTAAGHQKVKQQQLFNG
CCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
VKVYGHHLVVSSDGDIFGQAAAIEADFNVTPALTRGQAIAALNKVYPGAVASGHKDVELV
EEEEEEEEEEECCCCEECCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEE
ILAEGDAPRLAYRVSFLSEGVNEISRPAGLVDAMTGEVVRHWNELMTAKNSAGKPGNGGG
EEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TTPGTAFDATGPGGNAKTGQYYYGSDFGPLNVEEANGVCTMNNANVKTVDMANSTRRGSV
CCCCCEEECCCCCCCCCCCCEEECCCCCCCCEECCCCEEEECCCCEEEEECCCCCCCCCE
YSFTCPENTYRAINGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQLEMRVHYGRNYENA
EEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCC
FWDGRAMSFGDGASYFYPLVSLDVSAHEVSHGFTEQNSGLEYSAQSGGMNEAFSDMAGEA
CCCCCEEECCCCCCCEEEEEEECCCHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHH
AEFFMRGSNDWMVGHDIFKGNGALRYMDDPTKDGRSIGHASDYTSGMDVHYSSGVYNKAF
HHHHCCCCCCEEEECEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEE
YLLAKKNGWDTRKAFEVFVLANQHYWTATSNYNQGACGVEAAASDKGYAVSDVTAAFASV
EEEEECCCCCCCEEEEEEEEECCEEEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHC
GVSCQ
CCCCC
>Mature Secondary Structure
MKAYTLIPATLALCVSGALQAGNTLEHRMGLAHGNSFTAGKSFTTAAGHQKVKQQQLFNG
CCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
VKVYGHHLVVSSDGDIFGQAAAIEADFNVTPALTRGQAIAALNKVYPGAVASGHKDVELV
EEEEEEEEEEECCCCEECCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEE
ILAEGDAPRLAYRVSFLSEGVNEISRPAGLVDAMTGEVVRHWNELMTAKNSAGKPGNGGG
EEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TTPGTAFDATGPGGNAKTGQYYYGSDFGPLNVEEANGVCTMNNANVKTVDMANSTRRGSV
CCCCCEEECCCCCCCCCCCCEEECCCCCCCCEECCCCEEEECCCCEEEEECCCCCCCCCE
YSFTCPENTYRAINGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQLEMRVHYGRNYENA
EEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCC
FWDGRAMSFGDGASYFYPLVSLDVSAHEVSHGFTEQNSGLEYSAQSGGMNEAFSDMAGEA
CCCCCEEECCCCCCCEEEEEEECCCHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHH
AEFFMRGSNDWMVGHDIFKGNGALRYMDDPTKDGRSIGHASDYTSGMDVHYSSGVYNKAF
HHHHCCCCCCEEEECEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEE
YLLAKKNGWDTRKAFEVFVLANQHYWTATSNYNQGACGVEAAASDKGYAVSDVTAAFASV
EEEEECCCCCCCEEEEEEEEECCEEEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHC
GVSCQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2842313; 2493453; 1917848; 10984043; 1544509; 3034864; 2123831; 1899664 [H]