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The map label for this gene is menE [H]

Identifier: 119774321

GI number: 119774321

Start: 1447395

End: 1449089

Strand: Direct

Name: menE [H]

Synonym: Sama_1184

Alternate gene names: 119774321

Gene position: 1447395-1449089 (Clockwise)

Preceding gene: 119774320

Following gene: 119774322

Centisome position: 33.61

GC content: 55.34

Gene sequence:

>1695_bases
ATGAATGCCAACCTGTGCCGTCATTTGGTTTCGGCCGCACAGGCTCAGCCCCAGGGCTTGGCGGTGGCGGTACAAAGGCA
CAGATTCGGCAAGCCGCCGCGGGGAATTGGTGAGCTTTGCTATGATGAGCTGACCCTGAGTGAGCTGAATCGTCGCAGTG
ATGCCATTGCCCATGGCCTCAATGCCATAGGTCTCAATGCCGGTGACAAAGCCGTGCTTATGGTGACACCCGGGCTGGAT
TTTTTTGCTCTGACATTTGCGCTTTTTAAAGCCGGGATCATTCCCGTGATGGTCGACCCCGGCATGGGCATCAAAAACCT
TGGCCAGTGTTTCGATGAAGCAGCGCCCGATGCCTTTATCGGTATTCCCAAAGCCCATGTGGCGAGGATGCTGTTTTCCT
GGGGTAAAAAAACCGTCACTCAGTTGGTGACGGTTGGTCGGGGGCTCAAACTCTGGGGTGGCAATACTCTGGTGCAGATT
GAAAAGCACGGTCAGGATATGGGGCCCTATCCCATGACCTTGCTCGATGAGCAGGCCCTTTGCGCCATCCTTTTCACCAG
TGGCAGCACAGGCGTACCCAAGGGAGTTGAATACAGCCATCAGATGTTTGAGGCGCAAATTCAGGCGCTCAAACAGGATT
ATGGCATCCGCCACGGTGAGCGGGACTTATCGACTTTTCCGCTGTTTGCCCTCTTTGGTCCGGCATTGGGAATGGCCTCC
ATCGTGCCCTGTATGGATGCCAGTCGCCCCATCAAGGCCAAACCCGAATACCTGTTCAAGGCCATTGCCGACTACCAGTG
CACCAACCTGTTTTTGAATCCGGCACTGCTGGATAAGCTTGGCCGATACGGCGAGGCGAATGCACTGACACTGAATGGCG
TCAGGCGGGTTATCTCTGCCGGGGCTCCGGCATCCATTGACGCTATCAACCGCTTCCGGCAGATACTGCCGCCGGATGCG
CCTGTGCTGAATTCTTACGGCGCCACTGAAGGCCTGCCCCTGTGTTTTGTTGGCAGTGATGAGCTGCTCGCGAGCGGTGA
GGTGACAGCCAAAGGCGGTGGCATTCTGGTGGGTAAACCGGTGCAGGGCGTTGCGCTTGAAATCATCGCCATCGACGAGG
CGCCAATTGCCGAGTGGCAGGACGAGCTTAAACTTCAACCCTATGAAATCGGTGAAATAGTGGTAAAAGGCCCCATGGTA
AGCCGCGCTTACTATCACAGAGATGAAGCCACCAGTATCGCCAAAATTGCCGATGACGGAGAGTTCTGGCATCGCATGGG
TGATCTTGGGTATCTCGATGAGCTGGGCCGCCTGTGGATGTGTGGCCGTAAGGCCCATCGGGTGGATGCCACCGAAGGCG
GCGCCTTCAGTAAACGCTATTTTTCCATTCCCTGTGAGCGAATTTTCAATACTCATCCGCTGGTTGCCCGCTCGGCACTG
GTTGGGGTGCATCGTCATGGGGATAAGATCCCCTTGGTATGCCTTGAGCTCGAACGTTCTCAGGCCTGCAACAATGCCAG
CAGCCTGTACCGAGAGCTGCGTGAAATGGCCGAGGCCCACGAAATCACTCAGGGGATAGATTTCTTCCTTATCCATGAGT
CCTTCCCCATGGATGTTCGCCACAACGCCAAGATTTTCAGGGAAAAACTCGCGCTTTGGGCCGAAAAACAACTCTCTGGT
GGTCTGAATAAATGA

Upstream 100 bases:

>100_bases
AAGACTGTGGTCACTACATCCTTGAGGATGCCAGCGACGAAGTGATAGCCCACATTGAGCGTTTTGTGGCGGCCTCTGCG
CCTGCCGAGGCCCTTGCGTC

Downstream 100 bases:

>100_bases
CGCAGGCGTATCCTTTGCATCCGGCGCAGCTGGAGGCCGCCAAACGCCTTAAGACCCACGTCAGCCGCGTACTGGTGACA
GGCGCAGGGGGCTTTTTGGG

Product: peptide synthase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MNANLCRHLVSAAQAQPQGLAVAVQRHRFGKPPRGIGELCYDELTLSELNRRSDAIAHGLNAIGLNAGDKAVLMVTPGLD
FFALTFALFKAGIIPVMVDPGMGIKNLGQCFDEAAPDAFIGIPKAHVARMLFSWGKKTVTQLVTVGRGLKLWGGNTLVQI
EKHGQDMGPYPMTLLDEQALCAILFTSGSTGVPKGVEYSHQMFEAQIQALKQDYGIRHGERDLSTFPLFALFGPALGMAS
IVPCMDASRPIKAKPEYLFKAIADYQCTNLFLNPALLDKLGRYGEANALTLNGVRRVISAGAPASIDAINRFRQILPPDA
PVLNSYGATEGLPLCFVGSDELLASGEVTAKGGGILVGKPVQGVALEIIAIDEAPIAEWQDELKLQPYEIGEIVVKGPMV
SRAYYHRDEATSIAKIADDGEFWHRMGDLGYLDELGRLWMCGRKAHRVDATEGGAFSKRYFSIPCERIFNTHPLVARSAL
VGVHRHGDKIPLVCLELERSQACNNASSLYRELREMAEAHEITQGIDFFLIHESFPMDVRHNAKIFREKLALWAEKQLSG
GLNK

Sequences:

>Translated_564_residues
MNANLCRHLVSAAQAQPQGLAVAVQRHRFGKPPRGIGELCYDELTLSELNRRSDAIAHGLNAIGLNAGDKAVLMVTPGLD
FFALTFALFKAGIIPVMVDPGMGIKNLGQCFDEAAPDAFIGIPKAHVARMLFSWGKKTVTQLVTVGRGLKLWGGNTLVQI
EKHGQDMGPYPMTLLDEQALCAILFTSGSTGVPKGVEYSHQMFEAQIQALKQDYGIRHGERDLSTFPLFALFGPALGMAS
IVPCMDASRPIKAKPEYLFKAIADYQCTNLFLNPALLDKLGRYGEANALTLNGVRRVISAGAPASIDAINRFRQILPPDA
PVLNSYGATEGLPLCFVGSDELLASGEVTAKGGGILVGKPVQGVALEIIAIDEAPIAEWQDELKLQPYEIGEIVVKGPMV
SRAYYHRDEATSIAKIADDGEFWHRMGDLGYLDELGRLWMCGRKAHRVDATEGGAFSKRYFSIPCERIFNTHPLVARSAL
VGVHRHGDKIPLVCLELERSQACNNASSLYRELREMAEAHEITQGIDFFLIHESFPMDVRHNAKIFREKLALWAEKQLSG
GLNK
>Mature_564_residues
MNANLCRHLVSAAQAQPQGLAVAVQRHRFGKPPRGIGELCYDELTLSELNRRSDAIAHGLNAIGLNAGDKAVLMVTPGLD
FFALTFALFKAGIIPVMVDPGMGIKNLGQCFDEAAPDAFIGIPKAHVARMLFSWGKKTVTQLVTVGRGLKLWGGNTLVQI
EKHGQDMGPYPMTLLDEQALCAILFTSGSTGVPKGVEYSHQMFEAQIQALKQDYGIRHGERDLSTFPLFALFGPALGMAS
IVPCMDASRPIKAKPEYLFKAIADYQCTNLFLNPALLDKLGRYGEANALTLNGVRRVISAGAPASIDAINRFRQILPPDA
PVLNSYGATEGLPLCFVGSDELLASGEVTAKGGGILVGKPVQGVALEIIAIDEAPIAEWQDELKLQPYEIGEIVVKGPMV
SRAYYHRDEATSIAKIADDGEFWHRMGDLGYLDELGRLWMCGRKAHRVDATEGGAFSKRYFSIPCERIFNTHPLVARSAL
VGVHRHGDKIPLVCLELERSQACNNASSLYRELREMAEAHEITQGIDFFLIHESFPMDVRHNAKIFREKLALWAEKQLSG
GLNK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=470, Percent_Identity=23.8297872340426, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1789201, Length=286, Percent_Identity=27.6223776223776, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=404, Percent_Identity=22.029702970297, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=286, Percent_Identity=25.1748251748252, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=373, Percent_Identity=22.7882037533512, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18859661, Length=329, Percent_Identity=23.1003039513678, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 61633; Mature: 61633

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANLCRHLVSAAQAQPQGLAVAVQRHRFGKPPRGIGELCYDELTLSELNRRSDAIAHGL
CCHHHHHHHHHHHHCCCCCHHEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NAIGLNAGDKAVLMVTPGLDFFALTFALFKAGIIPVMVDPGMGIKNLGQCFDEAAPDAFI
CCEECCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEE
GIPKAHVARMLFSWGKKTVTQLVTVGRGLKLWGGNTLVQIEKHGQDMGPYPMTLLDEQAL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCHHHCCCCCE
CAILFTSGSTGVPKGVEYSHQMFEAQIQALKQDYGIRHGERDLSTFPLFALFGPALGMAS
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
IVPCMDASRPIKAKPEYLFKAIADYQCTNLFLNPALLDKLGRYGEANALTLNGVRRVISA
HHHHCCCCCCCCCCHHHHHHHHHCCHHHHEEECHHHHHHHCCCCCCCEEEHHHHHHHHHC
GAPASIDAINRFRQILPPDAPVLNSYGATEGLPLCFVGSDELLASGEVTAKGGGILVGKP
CCCCCHHHHHHHHHHCCCCCCHHCCCCCCCCCCEEEECCCHHEECCCEEECCCCEEECCC
VQGVALEIIAIDEAPIAEWQDELKLQPYEIGEIVVKGPMVSRAYYHRDEATSIAKIADDG
CCCEEEEEEEECCCCHHHHHHHHCCCCEECCEEEEECCCHHHHHHCCCHHHHHHHHCCCH
EFWHRMGDLGYLDELGRLWMCGRKAHRVDATEGGAFSKRYFSIPCERIFNTHPLVARSAL
HHHHHHCCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHCCCCHHHHCCCCCHHHHHHH
VGVHRHGDKIPLVCLELERSQACNNASSLYRELREMAEAHEITQGIDFFLIHESFPMDVR
HHHHHCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
HNAKIFREKLALWAEKQLSGGLNK
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNANLCRHLVSAAQAQPQGLAVAVQRHRFGKPPRGIGELCYDELTLSELNRRSDAIAHGL
CCHHHHHHHHHHHHCCCCCHHEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NAIGLNAGDKAVLMVTPGLDFFALTFALFKAGIIPVMVDPGMGIKNLGQCFDEAAPDAFI
CCEECCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEE
GIPKAHVARMLFSWGKKTVTQLVTVGRGLKLWGGNTLVQIEKHGQDMGPYPMTLLDEQAL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCHHHCCCCCE
CAILFTSGSTGVPKGVEYSHQMFEAQIQALKQDYGIRHGERDLSTFPLFALFGPALGMAS
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
IVPCMDASRPIKAKPEYLFKAIADYQCTNLFLNPALLDKLGRYGEANALTLNGVRRVISA
HHHHCCCCCCCCCCHHHHHHHHHCCHHHHEEECHHHHHHHCCCCCCCEEEHHHHHHHHHC
GAPASIDAINRFRQILPPDAPVLNSYGATEGLPLCFVGSDELLASGEVTAKGGGILVGKP
CCCCCHHHHHHHHHHCCCCCCHHCCCCCCCCCCEEEECCCHHEECCCEEECCCCEEECCC
VQGVALEIIAIDEAPIAEWQDELKLQPYEIGEIVVKGPMVSRAYYHRDEATSIAKIADDG
CCCEEEEEEEECCCCHHHHHHHHCCCCEECCEEEEECCCHHHHHHCCCHHHHHHHHCCCH
EFWHRMGDLGYLDELGRLWMCGRKAHRVDATEGGAFSKRYFSIPCERIFNTHPLVARSAL
HHHHHHCCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHCCCCHHHHCCCCCHHHHHHH
VGVHRHGDKIPLVCLELERSQACNNASSLYRELREMAEAHEITQGIDFFLIHESFPMDVR
HHHHHCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
HNAKIFREKLALWAEKQLSGGLNK
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA