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The map label for this gene is ybjS [C]

Identifier: 119774322

GI number: 119774322

Start: 1449086

End: 1450141

Strand: Direct

Name: ybjS [C]

Synonym: Sama_1185

Alternate gene names: 119774322

Gene position: 1449086-1450141 (Clockwise)

Preceding gene: 119774321

Following gene: 119774324

Centisome position: 33.65

GC content: 59.56

Gene sequence:

>1056_bases
ATGACGCAGGCGTATCCTTTGCATCCGGCGCAGCTGGAGGCCGCCAAACGCCTTAAGACCCACGTCAGCCGCGTACTGGT
GACAGGCGCAGGGGGCTTTTTGGGGCAGGCGCTGTGTCGGCAATTGCTCAGTGCCGGCATTGAGGTTGTCGGCATTGCCC
GCTCTGCCTATCCAGCCCTCGCAGCCATGGGGGTGGAGATGCACCGTGGCGACATTATGGACCTTAAGGCTTTGTCTGCT
GCGATGAACGGCTGCGAGCTGGTGTTTCATGTGGCGTCCAAGGCCGGGGTTTGGGGCAGCCGTGAAAGCTACTATGGCCC
CAATGTAACCGGGGCGGCCAATGTGCTGCAGGCAAGCCAGGATCTGGGTATCAAAGCCATTGTGTACACCAGTACTCCCA
GTGTGACCTTTGATGGCAAAGATGAATCGGGTATCGATGAGTCTGCCCCTTATGCGGCGCATTTTTTGAATCATTATGGT
GCATCCAAAGCGGAGGCGGAGGCCATGATGCTGCGGGCGAGCTCGCCGTCTCTGGTCATCACGGCGCTGCGGCCCCATCT
TATTTGGGGACCAAAGGATCCGCATCTGGTGCCCAGAGTGCTGGAGCGTGGCCGCGCCGGTCGTCTTCGCCTCCTGGGTG
CCGAAGACAAGCTGGTGGATACCATTTACGTGGACAACGCCGCCCATGCCCATGTACTGGCGGCGATTGCTTTGCTCGAA
AAGCCCGGCGAGTGTGGCGGCAGGGCATTTTTCCTCAGCAACGGCGAGCCGGTCACCATGGCGTCCATGCTGTCGAAAAT
TCTCGCCTGCGCAGAGCTGCCGGGCGTGACCCGTCGGGTACCTGTGTGGCTTGCTTACGGTATGGGCGCGGTTTTGGAAG
GAATGTATACGCTTCTTGGCAAACAGGACGAACCCCTGATGACCCGCTTTGTGGCCAGGCAGCTCTCTTGCAGCCACTAC
TACGATATCAGTGCCGCGCGGGAGATTTTAGACTACGAGCCGCTGATTTCTCTGGATGAGGGGATGGCGCGGCTCAAGGC
GTCACTTCAGCCGTAA

Upstream 100 bases:

>100_bases
TCCATGAGTCCTTCCCCATGGATGTTCGCCACAACGCCAAGATTTTCAGGGAAAAACTCGCGCTTTGGGCCGAAAAACAA
CTCTCTGGTGGTCTGAATAA

Downstream 100 bases:

>100_bases
CACCATTAATAAGCCCCCCAAATTAGCCCCAGGACGATTTGTCTCGGGGCTGACACAGTTGCTCAAACTGACTTCAAATG
TATTTTAAGCCCGGTAAGGC

Product: steroid dehydrogenase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTQAYPLHPAQLEAAKRLKTHVSRVLVTGAGGFLGQALCRQLLSAGIEVVGIARSAYPALAAMGVEMHRGDIMDLKALSA
AMNGCELVFHVASKAGVWGSRESYYGPNVTGAANVLQASQDLGIKAIVYTSTPSVTFDGKDESGIDESAPYAAHFLNHYG
ASKAEAEAMMLRASSPSLVITALRPHLIWGPKDPHLVPRVLERGRAGRLRLLGAEDKLVDTIYVDNAAHAHVLAAIALLE
KPGECGGRAFFLSNGEPVTMASMLSKILACAELPGVTRRVPVWLAYGMGAVLEGMYTLLGKQDEPLMTRFVARQLSCSHY
YDISAAREILDYEPLISLDEGMARLKASLQP

Sequences:

>Translated_351_residues
MTQAYPLHPAQLEAAKRLKTHVSRVLVTGAGGFLGQALCRQLLSAGIEVVGIARSAYPALAAMGVEMHRGDIMDLKALSA
AMNGCELVFHVASKAGVWGSRESYYGPNVTGAANVLQASQDLGIKAIVYTSTPSVTFDGKDESGIDESAPYAAHFLNHYG
ASKAEAEAMMLRASSPSLVITALRPHLIWGPKDPHLVPRVLERGRAGRLRLLGAEDKLVDTIYVDNAAHAHVLAAIALLE
KPGECGGRAFFLSNGEPVTMASMLSKILACAELPGVTRRVPVWLAYGMGAVLEGMYTLLGKQDEPLMTRFVARQLSCSHY
YDISAAREILDYEPLISLDEGMARLKASLQP
>Mature_350_residues
TQAYPLHPAQLEAAKRLKTHVSRVLVTGAGGFLGQALCRQLLSAGIEVVGIARSAYPALAAMGVEMHRGDIMDLKALSAA
MNGCELVFHVASKAGVWGSRESYYGPNVTGAANVLQASQDLGIKAIVYTSTPSVTFDGKDESGIDESAPYAAHFLNHYGA
SKAEAEAMMLRASSPSLVITALRPHLIWGPKDPHLVPRVLERGRAGRLRLLGAEDKLVDTIYVDNAAHAHVLAAIALLEK
PGECGGRAFFLSNGEPVTMASMLSKILACAELPGVTRRVPVWLAYGMGAVLEGMYTLLGKQDEPLMTRFVARQLSCSHYY
DISAAREILDYEPLISLDEGMARLKASLQP

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=357, Percent_Identity=31.0924369747899, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI8393516, Length=357, Percent_Identity=31.0924369747899, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI116268111, Length=349, Percent_Identity=29.2263610315186, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI239745448, Length=348, Percent_Identity=30.4597701149425, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI310132178, Length=348, Percent_Identity=30.4597701149425, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI310113012, Length=348, Percent_Identity=30.4597701149425, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI260763931, Length=347, Percent_Identity=27.9538904899135, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4504509, Length=347, Percent_Identity=27.9538904899135, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4504507, Length=353, Percent_Identity=27.1954674220963, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI19923621, Length=351, Percent_Identity=28.4900284900285, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87081792, Length=338, Percent_Identity=29.8816568047337, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17570557, Length=333, Percent_Identity=28.2282282282282, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI193211272, Length=337, Percent_Identity=27.0029673590504, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321437, Length=351, Percent_Identity=29.3447293447293, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 37612; Mature: 37481

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAYPLHPAQLEAAKRLKTHVSRVLVTGAGGFLGQALCRQLLSAGIEVVGIARSAYPAL
CCCCCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
AAMGVEMHRGDIMDLKALSAAMNGCELVFHVASKAGVWGSRESYYGPNVTGAANVLQASQ
HHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
DLGIKAIVYTSTPSVTFDGKDESGIDESAPYAAHFLNHYGASKAEAEAMMLRASSPSLVI
CCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHEEECCCCCEEE
TALRPHLIWGPKDPHLVPRVLERGRAGRLRLLGAEDKLVDTIYVDNAAHAHVLAAIALLE
EECCCEEEECCCCCCHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHH
KPGECGGRAFFLSNGEPVTMASMLSKILACAELPGVTRRVPVWLAYGMGAVLEGMYTLLG
CCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
KQDEPLMTRFVARQLSCSHYYDISAAREILDYEPLISLDEGMARLKASLQP
CCCCHHHHHHHHHHHCCCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQAYPLHPAQLEAAKRLKTHVSRVLVTGAGGFLGQALCRQLLSAGIEVVGIARSAYPAL
CCCCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
AAMGVEMHRGDIMDLKALSAAMNGCELVFHVASKAGVWGSRESYYGPNVTGAANVLQASQ
HHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
DLGIKAIVYTSTPSVTFDGKDESGIDESAPYAAHFLNHYGASKAEAEAMMLRASSPSLVI
CCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHEEECCCCCEEE
TALRPHLIWGPKDPHLVPRVLERGRAGRLRLLGAEDKLVDTIYVDNAAHAHVLAAIALLE
EECCCEEEECCCCCCHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHH
KPGECGGRAFFLSNGEPVTMASMLSKILACAELPGVTRRVPVWLAYGMGAVLEGMYTLLG
CCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
KQDEPLMTRFVARQLSCSHYYDISAAREILDYEPLISLDEGMARLKASLQP
CCCCHHHHHHHHHHHCCCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]