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The map label for this gene is purU [H]

Identifier: 119774271

GI number: 119774271

Start: 1383259

End: 1384104

Strand: Direct

Name: purU [H]

Synonym: Sama_1134

Alternate gene names: 119774271

Gene position: 1383259-1384104 (Clockwise)

Preceding gene: 119774270

Following gene: 119774276

Centisome position: 32.12

GC content: 50.83

Gene sequence:

>846_bases
GTGTTGCAACCATCAGATCGCCGGGTATTGATAACCGATTGTGCCGATGCCAAGGGCCTTATCGCCAAAATCACAGGTGT
GTGTTTTGAAAATGGCCTCAACATCATCAAAAACGACGAGTTTGTTGATAATACCGAGGGGCGCTTTTTTATGCGTACCG
AGCTGGAAGGCCTGGTAGATGAGGCGGGTTTGCTCGGCGCGCTCGATGAGGTGCTGCCCGCACACCATCACACCAACCTT
AAACCGGCCGGGCGTAAAAGAGTGGTGGTGTTGGTGACCAAGGAAGCTCACTGTCTGGGTGATTTATTGATGAAGGCTTA
TTATGGCGCTCTGGATGTGGATATTGCTGCAGTCGTGGGTAATTACGATAAACTCAGGCCACTCACGGAAAAGTTTGATA
TTCCTTTTCATTATGTCAGCCACGAAGGGCTGGACAGACATCAGCATGAAGCGGCGCTGGCTGAGGTCATTGCGCCCTAT
GGGCCCGATTATCTGGTGCTGGCCAAGTTTATGCGCATTCTGACACCGGAATTTGTTGCGCGATATCCCAATCGCATCAT
CAATATTCATCACTCGTTTTTGCCAGCCTTTATTGGTGCCAATCCCTACCGACAGGCTTGGGAGCGCGGCGTGAAGATAA
TCGGGGCTACGGCTCACTTTGTGAATAATTGCCTGGATGAAGGTCCTATCATCAAACAGGATGTCATTCACGTTGATCAC
AATTACAGTGCAGCAGAGATGGCAAGGGCTGGACGGGATGTGGAGAAATCGGTGCTGAGCCGGGCGCTGGGTTTGGTACT
GGCCGATAAGGTCGTGGTTTACGGTAACAAGACCGTGGTGTTTTAA

Upstream 100 bases:

>100_bases
CTCCCCATGTTTCTCTGATATCTGATAAAAATCAGGGTAATTCGCTATTAATACTGTGCCTGTGGCATGCTGCCCAAAAT
ACAACGAATAGGGGAAGTGT

Downstream 100 bases:

>100_bases
CACAGCACCCACCAAAAAGGCGCCTCGGCGCCTTTTTGTGCTACTTGTCGAACTCTACATAAAGCATTTCATCGGCGTGA
TTCATGGCCAAATCTTTGAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLQPSDRRVLITDCADAKGLIAKITGVCFENGLNIIKNDEFVDNTEGRFFMRTELEGLVDEAGLLGALDEVLPAHHHTNL
KPAGRKRVVVLVTKEAHCLGDLLMKAYYGALDVDIAAVVGNYDKLRPLTEKFDIPFHYVSHEGLDRHQHEAALAEVIAPY
GPDYLVLAKFMRILTPEFVARYPNRIINIHHSFLPAFIGANPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQDVIHVDH
NYSAAEMARAGRDVEKSVLSRALGLVLADKVVVYGNKTVVF

Sequences:

>Translated_281_residues
MLQPSDRRVLITDCADAKGLIAKITGVCFENGLNIIKNDEFVDNTEGRFFMRTELEGLVDEAGLLGALDEVLPAHHHTNL
KPAGRKRVVVLVTKEAHCLGDLLMKAYYGALDVDIAAVVGNYDKLRPLTEKFDIPFHYVSHEGLDRHQHEAALAEVIAPY
GPDYLVLAKFMRILTPEFVARYPNRIINIHHSFLPAFIGANPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQDVIHVDH
NYSAAEMARAGRDVEKSVLSRALGLVLADKVVVYGNKTVVF
>Mature_281_residues
MLQPSDRRVLITDCADAKGLIAKITGVCFENGLNIIKNDEFVDNTEGRFFMRTELEGLVDEAGLLGALDEVLPAHHHTNL
KPAGRKRVVVLVTKEAHCLGDLLMKAYYGALDVDIAAVVGNYDKLRPLTEKFDIPFHYVSHEGLDRHQHEAALAEVIAPY
GPDYLVLAKFMRILTPEFVARYPNRIINIHHSFLPAFIGANPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQDVIHVDH
NYSAAEMARAGRDVEKSVLSRALGLVLADKVVVYGNKTVVF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=200, Percent_Identity=30, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI209869995, Length=200, Percent_Identity=30, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI209869993, Length=200, Percent_Identity=30, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1787483, Length=275, Percent_Identity=68, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1788846, Length=88, Percent_Identity=46.5909090909091, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=157, Percent_Identity=36.9426751592357, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=29.2553191489362, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31248; Mature: 31248

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQPSDRRVLITDCADAKGLIAKITGVCFENGLNIIKNDEFVDNTEGRFFMRTELEGLVD
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEHHHHHHH
EAGLLGALDEVLPAHHHTNLKPAGRKRVVVLVTKEAHCLGDLLMKAYYGALDVDIAAVVG
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHC
NYDKLRPLTEKFDIPFHYVSHEGLDRHQHEAALAEVIAPYGPDYLVLAKFMRILTPEFVA
CCHHHCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHH
RYPNRIINIHHSFLPAFIGANPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQDVIHVDH
HCCHHHEEHHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCHHHEEEECC
NYSAAEMARAGRDVEKSVLSRALGLVLADKVVVYGNKTVVF
CCCHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEECCCEEEC
>Mature Secondary Structure
MLQPSDRRVLITDCADAKGLIAKITGVCFENGLNIIKNDEFVDNTEGRFFMRTELEGLVD
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEHHHHHHH
EAGLLGALDEVLPAHHHTNLKPAGRKRVVVLVTKEAHCLGDLLMKAYYGALDVDIAAVVG
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHC
NYDKLRPLTEKFDIPFHYVSHEGLDRHQHEAALAEVIAPYGPDYLVLAKFMRILTPEFVA
CCHHHCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHH
RYPNRIINIHHSFLPAFIGANPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQDVIHVDH
HCCHHHEEHHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCHHHEEEECC
NYSAAEMARAGRDVEKSVLSRALGLVLADKVVVYGNKTVVF
CCCHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]