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The map label for this gene is rpoS [H]

Identifier: 119774181

GI number: 119774181

Start: 1261552

End: 1262535

Strand: Direct

Name: rpoS [H]

Synonym: Sama_1044

Alternate gene names: 119774181

Gene position: 1261552-1262535 (Clockwise)

Preceding gene: 119774180

Following gene: 119774183

Centisome position: 29.3

GC content: 47.76

Gene sequence:

>984_bases
ATGAGCTTGAAAAACAATACGCTTGTTGCTGAAAAGCTTGTAGATTTTTCCGGAAAAGAAGCAGATACCGACACCAATAT
GGACGGTGAAGAAGTCAGTCTGGTTCAAGACCTGGAGCTGGAACAGCAGGTTCAGGATGATTTACAACGTAACCTTGATG
CCACTCAACTCTATTTGAGTGAAATAGGTTTTTCCCCTCTGCTGACCGCAGAAGAAGAAGTTTATTATTCCCGCAAAGCA
TTGCGCGGCTGTGAAAAGTCACGTAATCGCATGATAGAAAGCAATCTTCGGCTGGTAGTGAAAATAGCGCGTCGCTACAA
CAATCGTGGACTTGCTCTGCTTGACCTTATTGAAGAAGGTAACCTTGGCCTGATCCGGGCCGTTGAAAAGTTTGATCCTG
AGCGTGGATTCCGTTTCTCGACCTATGCCACCTGGTGGATACGCCAGACCATCGAACGGGCCATCATGAATCAAACCCGC
ACCATTCGTTTGCCCATTCATGTGGTTAAAGAACTCAATGTGTACCTGAGAACTGCCCGTGAGTTGGCGCATAAACTCGA
CCATGAGCCTACAGCAGAGGAAATTGCTGCCAAACTCAACCTTCCCAGCGTTGAAGTCAGCCGTATGCTCAAACTCAACG
AAAAAATTACCTCTGTCGATGTGCCACTGGGCGGTGACAACGATAAGGCGCTGTTGGACGTATTACCCGATGATGACTCT
GTTGGTCCTGACTATAAGGTACAGGACGAAGACATATCTAATTCGGTGGTGAACTGGCTCAATGAACTCAATACCAAGCA
GCGAGAAGTGTTGGCACGTCGTTTTGGATTACTCGGCTATGAGCCCTCAACCCTGGAAGATGTGGGTGCTGAAATCGGTC
TGACCCGTGAACGTGTAAGGCAAATACAGGTAGAAGCGCTTAAACGCTTAAGAGACATTCTGAGTGCTCAGGGGCTGTCG
GTTGAAGCTCTGTTCAGAACCTAA

Upstream 100 bases:

>100_bases
ATTTGCCGAAGAAATAATTTACGGCAGCCAATTTTGGCATTTGGAGGATAAAAAAGTGATTTAAAGTCATCAACAAATTT
TGGGTTTGGGAGACAATATT

Downstream 100 bases:

>100_bases
ACGATTAATGCAATGAAAAACGCCTCAATGAATGAGGCGTTTTTGTTTTATGGTGTCGGTGTTTTGAACACAACTGCCAG
TTAGCAGTCTGCTTTTGGCT

Product: RNA polymerase sigma-38 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSLKNNTLVAEKLVDFSGKEADTDTNMDGEEVSLVQDLELEQQVQDDLQRNLDATQLYLSEIGFSPLLTAEEEVYYSRKA
LRGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTR
TIRLPIHVVKELNVYLRTARELAHKLDHEPTAEEIAAKLNLPSVEVSRMLKLNEKITSVDVPLGGDNDKALLDVLPDDDS
VGPDYKVQDEDISNSVVNWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDILSAQGLS
VEALFRT

Sequences:

>Translated_327_residues
MSLKNNTLVAEKLVDFSGKEADTDTNMDGEEVSLVQDLELEQQVQDDLQRNLDATQLYLSEIGFSPLLTAEEEVYYSRKA
LRGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTR
TIRLPIHVVKELNVYLRTARELAHKLDHEPTAEEIAAKLNLPSVEVSRMLKLNEKITSVDVPLGGDNDKALLDVLPDDDS
VGPDYKVQDEDISNSVVNWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDILSAQGLS
VEALFRT
>Mature_326_residues
SLKNNTLVAEKLVDFSGKEADTDTNMDGEEVSLVQDLELEQQVQDDLQRNLDATQLYLSEIGFSPLLTAEEEVYYSRKAL
RGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRT
IRLPIHVVKELNVYLRTARELAHKLDHEPTAEEIAAKLNLPSVEVSRMLKLNEKITSVDVPLGGDNDKALLDVLPDDDSV
GPDYKVQDEDISNSVVNWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDILSAQGLSV
EALFRT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=282, Percent_Identity=79.7872340425532, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1789448, Length=271, Percent_Identity=39.8523985239852, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=28.2685512367491, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37294; Mature: 37163

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKNNTLVAEKLVDFSGKEADTDTNMDGEEVSLVQDLELEQQVQDDLQRNLDATQLYLS
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EIGFSPLLTAEEEVYYSRKALRGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEG
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCC
NLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTAR
CCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
ELAHKLDHEPTAEEIAAKLNLPSVEVSRMLKLNEKITSVDVPLGGDNDKALLDVLPDDDS
HHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEECCCCCC
VGPDYKVQDEDISNSVVNWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVR
CCCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHH
QIQVEALKRLRDILSAQGLSVEALFRT
HHHHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
SLKNNTLVAEKLVDFSGKEADTDTNMDGEEVSLVQDLELEQQVQDDLQRNLDATQLYLS
CCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EIGFSPLLTAEEEVYYSRKALRGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEG
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCC
NLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTAR
CCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
ELAHKLDHEPTAEEIAAKLNLPSVEVSRMLKLNEKITSVDVPLGGDNDKALLDVLPDDDS
HHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEECCCCCC
VGPDYKVQDEDISNSVVNWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVR
CCCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHH
QIQVEALKRLRDILSAQGLSVEALFRT
HHHHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]