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The map label for this gene is ispF

Identifier: 119774176

GI number: 119774176

Start: 1257628

End: 1258110

Strand: Direct

Name: ispF

Synonym: Sama_1039

Alternate gene names: 119774176

Gene position: 1257628-1258110 (Clockwise)

Preceding gene: 119774175

Following gene: 119774177

Centisome position: 29.21

GC content: 53.83

Gene sequence:

>483_bases
ATGAAAATCAGGATTGGCCACGGTTTTGATGTGCACAAGTTTGGCGCAGACCGTCCACTCATTCTCTGCGGTATAGAAGT
GCCTTACGAAACAGGACTTATTGCCCACTCCGATGGTGATGTAGTGCTGCATGCCATCAGTGATGCCATTCTGGGCGCCA
TGGCGCTGGGTGATATCGGCAAACACTTTCCGGACACAGATGCCGCCTATGAAGGCGCCGACAGCCGTGTGCTGCTGCGC
CACTGCTTTAAGCTTGCCCGTGAGCACGGCTTTGCCATCGGCAATCTGGATGTGACCATCATCGCCCAGGCGCCGAAAAT
GTTACCTCACATTGAGGCCATGCGCGCGGTGCTGGCGGACGATCTGCAAACTGAGCTTAATAATATCAACGTAAAAGCGA
CCACCACCGAGAAGCTTGGTTTCACCGGTCGCAAAGAAGGCATCGCCGTGGAAGCTGTGGTGTTGATGGAGAATGTGAAA
TGA

Upstream 100 bases:

>100_bases
CAGTGGCCGCGCCGACAATATCAAGGTCACCCACCCGGACGATTTACAGCTGGCGGGGCTTTTTCTTCAGGCCCAGCAGC
AACACACTTAAGGAATTTTC

Downstream 100 bases:

>100_bases
CTGAGCTGACTTATCTTTACGGCAAGCCGGCTGTAAGCGCCGATATCCGCACCCACAACAGCGATTTTCAGGTAAAGGAA
ATCCTGCCGTTCCTGCCCGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKIRIGHGFDVHKFGADRPLILCGIEVPYETGLIAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYEGADSRVLLR
HCFKLAREHGFAIGNLDVTIIAQAPKMLPHIEAMRAVLADDLQTELNNINVKATTTEKLGFTGRKEGIAVEAVVLMENVK

Sequences:

>Translated_160_residues
MKIRIGHGFDVHKFGADRPLILCGIEVPYETGLIAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYEGADSRVLLR
HCFKLAREHGFAIGNLDVTIIAQAPKMLPHIEAMRAVLADDLQTELNNINVKATTTEKLGFTGRKEGIAVEAVVLMENVK
>Mature_160_residues
MKIRIGHGFDVHKFGADRPLILCGIEVPYETGLIAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYEGADSRVLLR
HCFKLAREHGFAIGNLDVTIIAQAPKMLPHIEAMRAVLADDLQTELNNINVKATTTEKLGFTGRKEGIAVEAVVLMENVK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=65.5844155844156, Blast_Score=202, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_SHEAM (A1S4E0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926916.1
- ProteinModelPortal:   A1S4E0
- SMR:   A1S4E0
- STRING:   A1S4E0
- GeneID:   4603291
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1039
- NMPDR:   fig|326297.7.peg.979
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   A1S4E0
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17279; Mature: 17279

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRIGHGFDVHKFGADRPLILCGIEVPYETGLIAHSDGDVVLHAISDAILGAMALGDIG
CEEEECCCCCHHHCCCCCCEEEEEEECCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHH
KHFPDTDAAYEGADSRVLLRHCFKLAREHGFAIGNLDVTIIAQAPKMLPHIEAMRAVLAD
CCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHH
DLQTELNNINVKATTTEKLGFTGRKEGIAVEAVVLMENVK
HHHHHHHCCEEEEEEHHHCCCCCCCCCEEEEEEEEEECCC
>Mature Secondary Structure
MKIRIGHGFDVHKFGADRPLILCGIEVPYETGLIAHSDGDVVLHAISDAILGAMALGDIG
CEEEECCCCCHHHCCCCCCEEEEEEECCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHH
KHFPDTDAAYEGADSRVLLRHCFKLAREHGFAIGNLDVTIIAQAPKMLPHIEAMRAVLAD
CCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHH
DLQTELNNINVKATTTEKLGFTGRKEGIAVEAVVLMENVK
HHHHHHHCCEEEEEEHHHCCCCCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA