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The map label for this gene is eno

Identifier: 119774173

GI number: 119774173

Start: 1255111

End: 1256406

Strand: Direct

Name: eno

Synonym: Sama_1036

Alternate gene names: 119774173

Gene position: 1255111-1256406 (Clockwise)

Preceding gene: 119774172

Following gene: 119774174

Centisome position: 29.15

GC content: 55.25

Gene sequence:

>1296_bases
ATGGCTAAGATTATCAAAGTGATCGGTCGTGAAATCATGGATTCACGCGGCAACCCAACCGTAGAAGCCGAAGTACACCT
GGAAGGTGGCTTTGTGGGCATGGCAGCTGCGCCATCCGGTGCTTCTACCGGTAGCCGCGAAGCGCTGGAACTGCGTGACG
GCGACAAGGCCCGTTACCTGGGTAAAGGCGTGCTGAAGGCCGTTGACAACGTAAATGGTCCTATCCGTGAAGCCCTGCTG
GGTAAAGATGCCACCGCTCAGGCTGAGCTGGACGGCATCATGATCGCTCTGGACGGTACCGAGAACAAAGACAAGCTGGG
TGCCAACGCCATTCTGGCTGTGTCTCTGGCTGCCGCCAAGGCCGCTGCCGCCGCTAAAGGTATTCCACTTTACGCTCACA
TCGCTGAGCTGAACGGTACCCCAGGCCAGTACAGCATGCCTGTACCCATGATGAACATCCTTAACGGTGGTGAGCACGCT
GACAACAACGTGGACATCCAGGAGTTCATGGTACAGCCAGTGAGCGCCAAGAGCTTCCGTGAAGCCCTGCGTATGGGCGC
CGAAATCTTCCACAACCTGAAGAAAGTGCTGAAGGCAAAGGGTCTGAACACTGCTGTGGGTGACGAAGGTGGTTTCGCGC
CAAACCTGGCCTCCAACGCCGATGCACTGGCTGTTATCAAAGAGGCTGTTGAAGCCGCTGGTTATAAGCTGGGCACCGAC
GTGACTCTGGCTCTGGACTGCGCCGCCTCTGAATTCTACAAAGACGGTCAGTACGACCTGTCTGGCGAAGGCAAAGTATT
CAGCTCAAACGGTTTCTCTGACTTCCTCAAGTCACTGACCGAGCAGTATCCAATCGTGTCTATCGAAGACGGTCTGGACG
AGTCTGATTGGGAAGGTTGGGCATACCAGACCCAAATTCTTGGCGACAAGGTACAGCTGGTAGGTGACGACCTGTTTGTA
ACCAACACCAAGATCCTGAGCCGCGGTATTGAGCAGGGCATTGCCAACTCAATCCTCATCAAGTTCAACCAGATTGGTTC
TCTGACCGAAACTCTGGCTGCTATCCGCATGGCTAAAGAAGCCGGTTACACCGCCGTTATCTCTCACCGCTCCGGCGAAA
CCGAAGATGCGACCATTGCCGATCTGGCCGTAGGTACCGCTGCTGGTCAAATCAAGACTGGCTCTCTGTGCCGCTCTGAC
CGTGTTGCCAAGTACAACCAGCTGCTGCGTATCGAAGAGCAACTGGGTGAGAAAGCGCCATACCGCGGTCTGACTGAAAT
CAATGCCAAGGGCTAA

Upstream 100 bases:

>100_bases
GGGTGCATTCAATTTGGTTCATGAGCTCATGTAGCGCTGAGATGGGTATGGGTTTTTCTTTTCAACTTAACCTTTAATTC
AAGACTAAATCGAGGACATT

Downstream 100 bases:

>100_bases
TCGCCTGATATTGGTTAATTGAATTTACAGAAGGCGACTTATAATGCCACGGCCTGAACGAAATCAGCGCCAAGGATTAA
TCGCCTGATAAAATTTGATT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAKIIKVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKARYLGKGVLKAVDNVNGPIREALL
GKDATAQAELDGIMIALDGTENKDKLGANAILAVSLAAAKAAAAAKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHA
DNNVDIQEFMVQPVSAKSFREALRMGAEIFHNLKKVLKAKGLNTAVGDEGGFAPNLASNADALAVIKEAVEAAGYKLGTD
VTLALDCAASEFYKDGQYDLSGEGKVFSSNGFSDFLKSLTEQYPIVSIEDGLDESDWEGWAYQTQILGDKVQLVGDDLFV
TNTKILSRGIEQGIANSILIKFNQIGSLTETLAAIRMAKEAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSLCRSD
RVAKYNQLLRIEEQLGEKAPYRGLTEINAKG

Sequences:

>Translated_431_residues
MAKIIKVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKARYLGKGVLKAVDNVNGPIREALL
GKDATAQAELDGIMIALDGTENKDKLGANAILAVSLAAAKAAAAAKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHA
DNNVDIQEFMVQPVSAKSFREALRMGAEIFHNLKKVLKAKGLNTAVGDEGGFAPNLASNADALAVIKEAVEAAGYKLGTD
VTLALDCAASEFYKDGQYDLSGEGKVFSSNGFSDFLKSLTEQYPIVSIEDGLDESDWEGWAYQTQILGDKVQLVGDDLFV
TNTKILSRGIEQGIANSILIKFNQIGSLTETLAAIRMAKEAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSLCRSD
RVAKYNQLLRIEEQLGEKAPYRGLTEINAKG
>Mature_430_residues
AKIIKVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKARYLGKGVLKAVDNVNGPIREALLG
KDATAQAELDGIMIALDGTENKDKLGANAILAVSLAAAKAAAAAKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHAD
NNVDIQEFMVQPVSAKSFREALRMGAEIFHNLKKVLKAKGLNTAVGDEGGFAPNLASNADALAVIKEAVEAAGYKLGTDV
TLALDCAASEFYKDGQYDLSGEGKVFSSNGFSDFLKSLTEQYPIVSIEDGLDESDWEGWAYQTQILGDKVQLVGDDLFVT
NTKILSRGIEQGIANSILIKFNQIGSLTETLAAIRMAKEAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSLCRSDR
VAKYNQLLRIEEQLGEKAPYRGLTEINAKG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=53.6866359447005, Blast_Score=447, Evalue=1e-126,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=53.6866359447005, Blast_Score=447, Evalue=1e-126,
Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=52.9137529137529, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=53.0232558139535, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=48.6111111111111, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=27.6470588235294, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=27.6470588235294, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=27.6470588235294, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1789141, Length=431, Percent_Identity=81.9025522041763, Blast_Score=711, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=54.292343387471, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=54.292343387471, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=49.4736842105263, Blast_Score=191, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=50.5800464037123, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=49.8839907192575, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=49.8839907192575, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=49.8839907192575, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=49.8839907192575, Blast_Score=373, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=52.5581395348837, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=52.5581395348837, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=52.5581395348837, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=52.5581395348837, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=52.5581395348837, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=52.5581395348837, Blast_Score=407, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_SHEAM (A1S4D7)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926913.1
- ProteinModelPortal:   A1S4D7
- SMR:   A1S4D7
- STRING:   A1S4D7
- GeneID:   4603288
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1036
- NMPDR:   fig|326297.7.peg.975
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   A1S4D7
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45538; Mature: 45407

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 341-341 BINDING 159-159 BINDING 168-168 BINDING 289-289 BINDING 316-316 BINDING 341-341 BINDING 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIIKVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKARYL
CHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEECCCCCHHHHH
GKGVLKAVDNVNGPIREALLGKDATAQAELDGIMIALDGTENKDKLGANAILAVSLAAAK
HHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHH
AAAAAKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHADNNVDIQEFMVQPVSAKSFR
HHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRMGAEIFHNLKKVLKAKGLNTAVGDEGGFAPNLASNADALAVIKEAVEAAGYKLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
VTLALDCAASEFYKDGQYDLSGEGKVFSSNGFSDFLKSLTEQYPIVSIEDGLDESDWEGW
EEEEEEHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCCCC
AYQTQILGDKVQLVGDDLFVTNTKILSRGIEQGIANSILIKFNQIGSLTETLAAIRMAKE
EEEEHHCCCEEEEECCCEEEEHHHHHHHHHHHHCHHHEEEEEHHHCHHHHHHHHHHHHHH
AGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSLCRSDRVAKYNQLLRIEEQLGEKAP
CCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
YRGLTEINAKG
CCCCCCCCCCC
>Mature Secondary Structure 
AKIIKVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKARYL
HHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEECCCCCHHHHH
GKGVLKAVDNVNGPIREALLGKDATAQAELDGIMIALDGTENKDKLGANAILAVSLAAAK
HHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHH
AAAAAKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHADNNVDIQEFMVQPVSAKSFR
HHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRMGAEIFHNLKKVLKAKGLNTAVGDEGGFAPNLASNADALAVIKEAVEAAGYKLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
VTLALDCAASEFYKDGQYDLSGEGKVFSSNGFSDFLKSLTEQYPIVSIEDGLDESDWEGW
EEEEEEHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCCCC
AYQTQILGDKVQLVGDDLFVTNTKILSRGIEQGIANSILIKFNQIGSLTETLAAIRMAKE
EEEEHHCCCEEEEECCCEEEEHHHHHHHHHHHHCHHHEEEEEHHHCHHHHHHHHHHHHHH
AGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSLCRSDRVAKYNQLLRIEEQLGEKAP
CCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
YRGLTEINAKG
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA