Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 119774172

GI number: 119774172

Start: 1253255

End: 1254892

Strand: Direct

Name: pyrG

Synonym: Sama_1035

Alternate gene names: 119774172

Gene position: 1253255-1254892 (Clockwise)

Preceding gene: 119774171

Following gene: 119774173

Centisome position: 29.1

GC content: 54.09

Gene sequence:

>1638_bases
ATGACGACAAGGTATATCTTCGTGACTGGCGGCGTGGTTTCGTCGCTGGGCAAAGGCATTGCAGCAGCATCATTGGCAGC
CATTCTCGAGGCACGGGGCCTCAACGTGACCATCATGAAGCTGGATCCTTACATCAACGTTGACCCAGGTACCATGAGCC
CGACACAACACGGTGAAGTGTTTGTGACTGAGGACGGCGCAGAGACGGATCTGGACCTGGGTCACTATGAGCGTTTCATC
CGTACCAAGATGAACCGCCGTAACAACTTCACCACCGGTCGTATTTACGAAGAAGTGCTGCGTAAAGAGCGCCGTGGTGA
CTATCTGGGCGCTACCATTCAGGTGATCCCACACATCACCAACGCCATCAAGGCTAAGGTGCTGGAAGGCGGTGAAGGCC
ATGACGTGGCCATCGTGGAAATCGGTGGTACCGTGGGTGATATCGAATCCCTGCCGTTCCTCGAATCTATCCGTCAGCTG
GGCGTTGAACTGGGCCGCGATCGCTCTATGTTCATGCACCTGACTCTGGTGCCATTCCTCGGTGCTGCCGGCGAGGTGAA
AACCAAGCCTACCCAGCACTCGGTAAAAGAACTGCGTTCTATCGGTATTGCGCCTGATATTCTGGTTTGCCGCGGTGACC
GCGCCATTCCTGCCAACGAGCGGGCCAAGATTTCCCTGTTCTGTAACGTGGAAGAGCGCGCTGTTATCTCTCTGAAAGAC
GTTGACTCCATCTACAAGATCCCGGCACTCCTGACCTCTCAGGGACTGGATGACCTGGTCTGCAAGCGTTTCCACTTGGA
ATGCAAAGAAGCAGACCTGTCTGAGTGGGAAAACGTGATTTATCAGGAAGCCAATCCCAACGGTGAAATCACCATTGGTA
TGGTGGGCAAGTACATCGAGCTGCCAGACGCCTATAAGTCAGTGAACGAGGCGCTCAAGCACGCGGGCCTGAAAAACCGC
GTGACGGTTAACATCAAGTACATAGATTCACAAAGCGTAGAGGCCAAAGGCGATGAAGCCCTGGCTGGCCTCGACGGTAT
TCTGGTGCCGGGCGGTTTCGGTGAGCGTGGTGTTGAAGGTAAAATCCTTGCCGCCAAGTATGCCCGTGAAAATGAGCTGC
CTTACTTTGGTATTTGCCTCGGTATGCAGGTAGCCCTTATCGAGTTCGCCCGTAACGTGGCAGGTCTCACAGATGCGCAC
TCTACCGAGTTCAATAAGCAAACTCCGCATCCGGTTGTGGGTCTGATCACAGAATGGATTGACGAAGAAGGTAATATTGA
GCAGCGCGACGAAGCGTCCGATCTGGGCGGCACCATGCGCCTTGGCGCACAGTTGTGTCACCTCAAGGCAGGTACCAAGG
CTGCTGAAGCTTACGGCTCCGAGAGTTGTATCGAGCGTCATCGTCACCGCTATGAAGTGAACAATCACTATGTGAAGCAG
TTGGAAAAAGCCGGTCTGGTATTCAGTGGCCTGTCTTCTGACCGCAAACTGGTGGAAATGATTGAACTGCCAAACCACCC
ATGGTTTGTGGCTGGTCAGTTCCATCCAGAGTTTACTTCAACACCCCGTGACGGACATCCGCTGTTCGAAGGTTTTGTGG
CAGCAGCCAGAGCGCACCAAAAGCGCAGCCTGAGCTAA

Upstream 100 bases:

>100_bases
TGAGGTTTGTCGAAAGCACAGGGCTTTGATATACTGTCGCCCCGTCCTGGTGCTTTCTTACAAGCACATATTTTCACACT
CATATTTTCTCAGGTTCAGC

Downstream 100 bases:

>100_bases
TGCCAAGAGGCCGGTACCGCGATGGCAGTACCGGCCTTTTTGTTCCTTGTGGTTAATTATTCGCCGACACTAAATAAGCA
ATGGCACAAAAACACAGAAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKAKVLEGGEGHDVAIVEIGGTVGDIESLPFLESIRQL
GVELGRDRSMFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDILVCRGDRAIPANERAKISLFCNVEERAVISLKD
VDSIYKIPALLTSQGLDDLVCKRFHLECKEADLSEWENVIYQEANPNGEITIGMVGKYIELPDAYKSVNEALKHAGLKNR
VTVNIKYIDSQSVEAKGDEALAGLDGILVPGGFGERGVEGKILAAKYARENELPYFGICLGMQVALIEFARNVAGLTDAH
STEFNKQTPHPVVGLITEWIDEEGNIEQRDEASDLGGTMRLGAQLCHLKAGTKAAEAYGSESCIERHRHRYEVNNHYVKQ
LEKAGLVFSGLSSDRKLVEMIELPNHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAARAHQKRSLS

Sequences:

>Translated_545_residues
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKAKVLEGGEGHDVAIVEIGGTVGDIESLPFLESIRQL
GVELGRDRSMFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDILVCRGDRAIPANERAKISLFCNVEERAVISLKD
VDSIYKIPALLTSQGLDDLVCKRFHLECKEADLSEWENVIYQEANPNGEITIGMVGKYIELPDAYKSVNEALKHAGLKNR
VTVNIKYIDSQSVEAKGDEALAGLDGILVPGGFGERGVEGKILAAKYARENELPYFGICLGMQVALIEFARNVAGLTDAH
STEFNKQTPHPVVGLITEWIDEEGNIEQRDEASDLGGTMRLGAQLCHLKAGTKAAEAYGSESCIERHRHRYEVNNHYVKQ
LEKAGLVFSGLSSDRKLVEMIELPNHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAARAHQKRSLS
>Mature_544_residues
TTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIR
TKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKAKVLEGGEGHDVAIVEIGGTVGDIESLPFLESIRQLG
VELGRDRSMFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDILVCRGDRAIPANERAKISLFCNVEERAVISLKDV
DSIYKIPALLTSQGLDDLVCKRFHLECKEADLSEWENVIYQEANPNGEITIGMVGKYIELPDAYKSVNEALKHAGLKNRV
TVNIKYIDSQSVEAKGDEALAGLDGILVPGGFGERGVEGKILAAKYARENELPYFGICLGMQVALIEFARNVAGLTDAHS
TEFNKQTPHPVVGLITEWIDEEGNIEQRDEASDLGGTMRLGAQLCHLKAGTKAAEAYGSESCIERHRHRYEVNNHYVKQL
EKAGLVFSGLSSDRKLVEMIELPNHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAARAHQKRSLS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=44.3447037701975, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=557, Percent_Identity=45.2423698384201, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI28559083, Length=557, Percent_Identity=45.2423698384201, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI221316689, Length=557, Percent_Identity=45.2423698384201, Blast_Score=472, Evalue=1e-133,
Organism=Escherichia coli, GI1789142, Length=543, Percent_Identity=77.3480662983425, Blast_Score=856, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=608, Percent_Identity=38.8157894736842, Blast_Score=427, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322563, Length=572, Percent_Identity=43.1818181818182, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6319432, Length=568, Percent_Identity=42.0774647887324, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24664469, Length=557, Percent_Identity=44.3447037701975, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI21357815, Length=502, Percent_Identity=43.0278884462151, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_SHEAM (A1S4D6)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926912.1
- ProteinModelPortal:   A1S4D6
- SMR:   A1S4D6
- STRING:   A1S4D6
- GeneID:   4603287
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1035
- NMPDR:   fig|326297.7.peg.974
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   NIKIDPY
- PhylomeDB:   A1S4D6
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59929; Mature: 59798

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 379-379 ACT_SITE 515-515 ACT_SITE 517-517

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CCEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKAKVLEGGEGHDVAIVEIGGTVGDIESLPFLESIRQLGVELGRDRSMFMHLTLVPFL
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHC
GAAGEVKTKPTQHSVKELRSIGIAPDILVCRGDRAIPANERAKISLFCNVEERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEHH
VDSIYKIPALLTSQGLDDLVCKRFHLECKEADLSEWENVIYQEANPNGEITIGMVGKYIE
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCCCCEEEEEECCEEC
LPDAYKSVNEALKHAGLKNRVTVNIKYIDSQSVEAKGDEALAGLDGILVPGGFGERGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHCCCCEEECCCCCCCCCCC
KILAAKYARENELPYFGICLGMQVALIEFARNVAGLTDAHSTEFNKQTPHPVVGLITEWI
CEEEEEHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
DEEGNIEQRDEASDLGGTMRLGAQLCHLKAGTKAAEAYGSESCIERHRHRYEVNNHYVKQ
CCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHH
LEKAGLVFSGLSSDRKLVEMIELPNHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAARAHQ
HHHCCCEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
KRSLS
HCCCC
>Mature Secondary Structure 
TTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKAKVLEGGEGHDVAIVEIGGTVGDIESLPFLESIRQLGVELGRDRSMFMHLTLVPFL
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHC
GAAGEVKTKPTQHSVKELRSIGIAPDILVCRGDRAIPANERAKISLFCNVEERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEHH
VDSIYKIPALLTSQGLDDLVCKRFHLECKEADLSEWENVIYQEANPNGEITIGMVGKYIE
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCCCCEEEEEECCEEC
LPDAYKSVNEALKHAGLKNRVTVNIKYIDSQSVEAKGDEALAGLDGILVPGGFGERGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHCCCCEEECCCCCCCCCCC
KILAAKYARENELPYFGICLGMQVALIEFARNVAGLTDAHSTEFNKQTPHPVVGLITEWI
CEEEEEHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
DEEGNIEQRDEASDLGGTMRLGAQLCHLKAGTKAAEAYGSESCIERHRHRYEVNNHYVKQ
CCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHH
LEKAGLVFSGLSSDRKLVEMIELPNHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAARAHQ
HHHCCCEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
KRSLS
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA