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The map label for this gene is prfC [H]

Identifier: 119774105

GI number: 119774105

Start: 1171131

End: 1172717

Strand: Direct

Name: prfC [H]

Synonym: Sama_0968

Alternate gene names: 119774105

Gene position: 1171131-1172717 (Clockwise)

Preceding gene: 119774104

Following gene: 119774106

Centisome position: 27.2

GC content: 53.12

Gene sequence:

>1587_bases
ATGTCAAATACATTGCTTGCCGAAGTGGCTTCGCGCCGCACCTTCGCCATTATTTCTCACCCGGACGCCGGTAAAACCAC
CATTACCGAAAAGGTATTGTTGCACGGCCGCGCCATTCAAAGCGCCGGTACTGTAAAGGGGCGGGGCAGCGGTCAGCATG
CCAAGTCTGACTGGATGGAAATGGAAAAAGAGCGGGGTATTTCGGTTACCACCTCTGTGATGCAGTTTCCTTACAACAGC
TGCCTGGTGAACCTGCTCGATACCCCAGGTCACGAAGACTTCTCGGAAGATACCTACCGTACCCTGACGGCGGTAGATTC
CTGTTTGATGGTGATTGATGCCGCCAAGGGTGTGGAAGACCGTACCCGCAAGCTGATGGAAGTCACCCGTCTGCGTGATA
CGCCCATCGTGACCTTTATGAACAAGCTTGACCGTGATATCCGCGATCCCATGGAGCTGCTCGACGAGGTGGAGACAGAG
CTTAACATCCTCTGCGCCCCCATCACCTGGCCTATTGGTTGCGGTAAATTGTTCAAGGGCGTTTATCACCTGCACCGTGA
TGAAACCATCCTGTATCAGACTGGCCATGGCCATACCATTCAGGAACTGCGCATTGTAAAAGGCCTGGATAATCCGGATC
TGGATGCTGCTGTTGGCAGTGACTTTGCCGAGCAGTTGCGTGAAGAGCTGGAGCTGGTGCGCGGCGCCTCCAATGAGTTC
GACAAGGAACTGTTTTTGAGTGGTGAGCTGACACCGGTTTATTTCGGTACCGCCCTGGGTAACTTTGGTGTAGACCATAT
GCTTGATGGCTTGACCGAATGGGCGCCTGCACCCATGCCACGTGAGACCAGCGAGCGCACCGTGGAAGCCGGGGAGGACA
AGTTCTCGGGCTTCGTGTTTAAAATTCAGGCCAACATGGATCCCAAGCACCGTGACCGTATCGCCTTTATGCGCATAGTG
TCGGGCAAGTACACCCAGGGCATGAAGATGAAGCACGTGCGCATTGGCAAAACCGTGAACATCTCAGATGCCGTGACTTT
TATGGCCGGTGATCGTGAGCGCGCCGAAGAAGCCTTTGCGGGCGATATTATCGGTCTGCACAACCACGGCACCATCCAGA
TTGGTGATACCTTTACCCAGGGTGAAGATCTCAAGTTCACCGGTATTCCCAACTTTGCACCTGAAATGTTCCGCCGTATT
CGTCTGAAAGATCCGTTGAAGCAAAAGCAGCTGCTTAAGGGCCTGGTACAACTTTCTGAAGAAGGTGCCGTACAGGTGTT
CCGCCCGCTGGACACCAACGATCTTATCGTTGGGGCTGTGGGGGTGCTGCAGTTTGAAGTGGTAGTTGCCCGCCTCAAGT
CCGAATATAACGTTGAAGCTATCTACGAGGCGGTCAACGTGGCGACAGCGCGTTGGGTCTATTGTGAAGATGGGCGTAAA
CTGGAAGAATTCAAACGTAAGTGTTCCACCAATCTGGCATTGGATGGTGGTGACAATCTCACTTACATTGCCCCCACTAT
GGTTAATCTGAATCTGTCGATGGAACGTTATCCCGACATTCAGTTTGCCAAGACCCGCGAGCATTAA

Upstream 100 bases:

>100_bases
TCGTTGTACTCAAGGCCAATTTAGGCGAAAATACCCCCCAATTTAGTGCCATTCGAGTCGCCGCCTTCGGGCGGCGACGC
TACTTGAAAGAAAGCTATCC

Downstream 100 bases:

>100_bases
TCGCGGAACCAAAAGGCGGGGATACCCGCCTTTTTTATTGCCTGTTGTCTGGAGGTGAGTATGTTGTCCTGGATAAAGCG
TGGTTGGAAAGCGTATACGG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSNTLLAEVASRRTFAIISHPDAGKTTITEKVLLHGRAIQSAGTVKGRGSGQHAKSDWMEMEKERGISVTTSVMQFPYNS
CLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDRDIRDPMELLDEVETE
LNILCAPITWPIGCGKLFKGVYHLHRDETILYQTGHGHTIQELRIVKGLDNPDLDAAVGSDFAEQLREELELVRGASNEF
DKELFLSGELTPVYFGTALGNFGVDHMLDGLTEWAPAPMPRETSERTVEAGEDKFSGFVFKIQANMDPKHRDRIAFMRIV
SGKYTQGMKMKHVRIGKTVNISDAVTFMAGDRERAEEAFAGDIIGLHNHGTIQIGDTFTQGEDLKFTGIPNFAPEMFRRI
RLKDPLKQKQLLKGLVQLSEEGAVQVFRPLDTNDLIVGAVGVLQFEVVVARLKSEYNVEAIYEAVNVATARWVYCEDGRK
LEEFKRKCSTNLALDGGDNLTYIAPTMVNLNLSMERYPDIQFAKTREH

Sequences:

>Translated_528_residues
MSNTLLAEVASRRTFAIISHPDAGKTTITEKVLLHGRAIQSAGTVKGRGSGQHAKSDWMEMEKERGISVTTSVMQFPYNS
CLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDRDIRDPMELLDEVETE
LNILCAPITWPIGCGKLFKGVYHLHRDETILYQTGHGHTIQELRIVKGLDNPDLDAAVGSDFAEQLREELELVRGASNEF
DKELFLSGELTPVYFGTALGNFGVDHMLDGLTEWAPAPMPRETSERTVEAGEDKFSGFVFKIQANMDPKHRDRIAFMRIV
SGKYTQGMKMKHVRIGKTVNISDAVTFMAGDRERAEEAFAGDIIGLHNHGTIQIGDTFTQGEDLKFTGIPNFAPEMFRRI
RLKDPLKQKQLLKGLVQLSEEGAVQVFRPLDTNDLIVGAVGVLQFEVVVARLKSEYNVEAIYEAVNVATARWVYCEDGRK
LEEFKRKCSTNLALDGGDNLTYIAPTMVNLNLSMERYPDIQFAKTREH
>Mature_527_residues
SNTLLAEVASRRTFAIISHPDAGKTTITEKVLLHGRAIQSAGTVKGRGSGQHAKSDWMEMEKERGISVTTSVMQFPYNSC
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDRDIRDPMELLDEVETEL
NILCAPITWPIGCGKLFKGVYHLHRDETILYQTGHGHTIQELRIVKGLDNPDLDAAVGSDFAEQLREELELVRGASNEFD
KELFLSGELTPVYFGTALGNFGVDHMLDGLTEWAPAPMPRETSERTVEAGEDKFSGFVFKIQANMDPKHRDRIAFMRIVS
GKYTQGMKMKHVRIGKTVNISDAVTFMAGDRERAEEAFAGDIIGLHNHGTIQIGDTFTQGEDLKFTGIPNFAPEMFRRIR
LKDPLKQKQLLKGLVQLSEEGAVQVFRPLDTNDLIVGAVGVLQFEVVVARLKSEYNVEAIYEAVNVATARWVYCEDGRKL
EEFKRKCSTNLALDGGDNLTYIAPTMVNLNLSMERYPDIQFAKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=494, Percent_Identity=26.9230769230769, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI19923640, Length=516, Percent_Identity=25.7751937984496, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI25306283, Length=400, Percent_Identity=27.25, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI25306287, Length=488, Percent_Identity=24.7950819672131, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=29.6511627906977, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=31.9727891156463, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI310132016, Length=124, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=124, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=124, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI4503483, Length=158, Percent_Identity=26.5822784810127, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790835, Length=523, Percent_Identity=84.5124282982792, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=520, Percent_Identity=25.9615384615385, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI48994988, Length=211, Percent_Identity=31.2796208530806, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.6923076923077, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=488, Percent_Identity=27.2540983606557, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=161, Percent_Identity=32.9192546583851, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=32.2368421052632, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=27.8195488721804, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=27.8195488721804, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=476, Percent_Identity=26.6806722689076, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=421, Percent_Identity=25.8907363420428, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=28.4810126582279, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=28.4810126582279, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=25.1655629139073, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=27.5933609958506, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI221458488, Length=504, Percent_Identity=24.8015873015873, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI78706572, Length=156, Percent_Identity=33.974358974359, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59198; Mature: 59067

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTLLAEVASRRTFAIISHPDAGKTTITEKVLLHGRAIQSAGTVKGRGSGQHAKSDWME
CCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHH
MEKERGISVTTSVMQFPYNSCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVED
HHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
RTRKLMEVTRLRDTPIVTFMNKLDRDIRDPMELLDEVETELNILCAPITWPIGCGKLFKG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHH
VYHLHRDETILYQTGHGHTIQELRIVKGLDNPDLDAAVGSDFAEQLREELELVRGASNEF
HHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCC
DKELFLSGELTPVYFGTALGNFGVDHMLDGLTEWAPAPMPRETSERTVEAGEDKFSGFVF
CCCEEECCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCEEE
KIQANMDPKHRDRIAFMRIVSGKYTQGMKMKHVRIGKTVNISDAVTFMAGDRERAEEAFA
EEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEHHHHEEEEECCHHHHHHHHH
GDIIGLHNHGTIQIGDTFTQGEDLKFTGIPNFAPEMFRRIRLKDPLKQKQLLKGLVQLSE
CCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
EGAVQVFRPLDTNDLIVGAVGVLQFEVVVARLKSEYNVEAIYEAVNVATARWVYCEDGRK
CCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCH
LEEFKRKCSTNLALDGGDNLTYIAPTMVNLNLSMERYPDIQFAKTREH
HHHHHHHHCCCEEEECCCCEEEEEEEEEEEEECHHHCCCCEECCCCCC
>Mature Secondary Structure 
SNTLLAEVASRRTFAIISHPDAGKTTITEKVLLHGRAIQSAGTVKGRGSGQHAKSDWME
CCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHH
MEKERGISVTTSVMQFPYNSCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVED
HHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
RTRKLMEVTRLRDTPIVTFMNKLDRDIRDPMELLDEVETELNILCAPITWPIGCGKLFKG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHH
VYHLHRDETILYQTGHGHTIQELRIVKGLDNPDLDAAVGSDFAEQLREELELVRGASNEF
HHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCC
DKELFLSGELTPVYFGTALGNFGVDHMLDGLTEWAPAPMPRETSERTVEAGEDKFSGFVF
CCCEEECCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCEEE
KIQANMDPKHRDRIAFMRIVSGKYTQGMKMKHVRIGKTVNISDAVTFMAGDRERAEEAFA
EEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEHHHHEEEEECCHHHHHHHHH
GDIIGLHNHGTIQIGDTFTQGEDLKFTGIPNFAPEMFRRIRLKDPLKQKQLLKGLVQLSE
CCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
EGAVQVFRPLDTNDLIVGAVGVLQFEVVVARLKSEYNVEAIYEAVNVATARWVYCEDGRK
CCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCH
LEEFKRKCSTNLALDGGDNLTYIAPTMVNLNLSMERYPDIQFAKTREH
HHHHHHHHCCCEEEECCCCEEEEEEEEEEEEECHHHCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA