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The map label for this gene is dosP [H]

Identifier: 119773946

GI number: 119773946

Start: 993354

End: 994589

Strand: Direct

Name: dosP [H]

Synonym: Sama_0809

Alternate gene names: 119773946

Gene position: 993354-994589 (Clockwise)

Preceding gene: 119773941

Following gene: 119773947

Centisome position: 23.07

GC content: 54.61

Gene sequence:

>1236_bases
ATGCCGTTGAATGTGCTCATCATCGACGATTCACTCAGTTACTGCCAGCTGTTATCCAAATATTTCAGTGAACTGAATGT
GACCAGCGTGGACTACTGCCATGATGGTTCAGAGGCGTTGCAGCGCCTTCGGGAGCGCAGGGACAGATACCAGATTTTGG
TGGTGGATTTACATATGCCGGGGATGGACGGCATTGAACTTTTATCAAAGCTGTCGGGCCTTGAATACCGGGGCGGCGTC
ATTATCGCTTCGGGGATGGAGAGCCGCATCATAGAAGCCGCCTCTCAGATTGTGCTTGGGGCCCATTTGCGGCTGCTGGG
CGCCTTGATGAAGCCGGTATGTAAAACACAGCTGAACATCTGCATAGAGCGCCTGTTGATGATGGTGCCGGAGCTGAACA
CGTCCAAACCGGCCATGACCTTACAATCGCTTCGGGAATCCATTAAGCAAAATAAAGTGGTGCCTTACTACCAGCCCCAG
GTGGAAGTGGGGACCGGACGCATCGAAGGGTTTGAGGTGCTTTGCCGCATGCAGCTGGGGGCGCAATTGCAGTTGATGAC
CCCGGATCGTTTTATGGCGCTGGCCGAGCGCTACAACCTGCTCAATGCGGTGTCTGAACAGCTGATAGGGACAGCATTGA
AGGAGTGGGCCGAAATTGGCCTTCAGCCCGAATGCCGTCACGCCCGCTTGTCTATCAACCTCACACCCTCCCAGCTTGGC
CAGAGTTATTGGCCCCGCCGACTGCTGAGTTTTTGCCACAAATGGCAGGTGGATATCAGCAGGGTGACGGTGGAAATTAC
AGAAAATCAGGCGCTGGATAAGCAGTCGCAACATTCGGGGATTTCCCGGCTGCGCCTGCACAATTTTGGCGTTGCCATTG
ATGATTTCGGGACAGGCTACACCAACTTAACTCAGCTGTGCCGTCTGCCAATCAGTGAGCTTAAGCTGGACATAAGTCTG
GTGCGCGGAATTCACATGGACCCATTGGCACAGACCATTCTGAAGTCGTTGCTGGACATCGGGCGCCAATTGGGTGTGTC
TGTGGTGGCCGAAGGCGTGGAAGACCCAAGAGACTATCGATACCTTGAGTCTGTTCCGGGCCTGATAGTTCAGGGCTTTT
TGGTATGCAGGCCCAAGCCCCTGGGTGAATTGGTTCGTTGGTTGAAGGCCCGACAGCGGGTCGCCGTTGACACTAAGGGG
CTCGAGGCAGGCTCGAGTGCGACATACCGTCATTGA

Upstream 100 bases:

>100_bases
GACATCAGGGACGTTACCTGAAACCGAATGTCCATCATGGGTTTCACGGATGAAACTGATTTTTTCGTGGCCCGGTACCC
AGACTGTCCGGAGTCATATT

Downstream 100 bases:

>100_bases
GGGCAATATAAGGCGCCCAAATGACGTGCACGCCTGTGCCTTCCAGTGTGTCCGCAAAGGCTGAGGTCAAGCAGTTCCTG
TCCATGGCAATAATGCTTTT

Product: putative diguanylate phosphodiesterase

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MPLNVLIIDDSLSYCQLLSKYFSELNVTSVDYCHDGSEALQRLRERRDRYQILVVDLHMPGMDGIELLSKLSGLEYRGGV
IIASGMESRIIEAASQIVLGAHLRLLGALMKPVCKTQLNICIERLLMMVPELNTSKPAMTLQSLRESIKQNKVVPYYQPQ
VEVGTGRIEGFEVLCRMQLGAQLQLMTPDRFMALAERYNLLNAVSEQLIGTALKEWAEIGLQPECRHARLSINLTPSQLG
QSYWPRRLLSFCHKWQVDISRVTVEITENQALDKQSQHSGISRLRLHNFGVAIDDFGTGYTNLTQLCRLPISELKLDISL
VRGIHMDPLAQTILKSLLDIGRQLGVSVVAEGVEDPRDYRYLESVPGLIVQGFLVCRPKPLGELVRWLKARQRVAVDTKG
LEAGSSATYRH

Sequences:

>Translated_411_residues
MPLNVLIIDDSLSYCQLLSKYFSELNVTSVDYCHDGSEALQRLRERRDRYQILVVDLHMPGMDGIELLSKLSGLEYRGGV
IIASGMESRIIEAASQIVLGAHLRLLGALMKPVCKTQLNICIERLLMMVPELNTSKPAMTLQSLRESIKQNKVVPYYQPQ
VEVGTGRIEGFEVLCRMQLGAQLQLMTPDRFMALAERYNLLNAVSEQLIGTALKEWAEIGLQPECRHARLSINLTPSQLG
QSYWPRRLLSFCHKWQVDISRVTVEITENQALDKQSQHSGISRLRLHNFGVAIDDFGTGYTNLTQLCRLPISELKLDISL
VRGIHMDPLAQTILKSLLDIGRQLGVSVVAEGVEDPRDYRYLESVPGLIVQGFLVCRPKPLGELVRWLKARQRVAVDTKG
LEAGSSATYRH
>Mature_410_residues
PLNVLIIDDSLSYCQLLSKYFSELNVTSVDYCHDGSEALQRLRERRDRYQILVVDLHMPGMDGIELLSKLSGLEYRGGVI
IASGMESRIIEAASQIVLGAHLRLLGALMKPVCKTQLNICIERLLMMVPELNTSKPAMTLQSLRESIKQNKVVPYYQPQV
EVGTGRIEGFEVLCRMQLGAQLQLMTPDRFMALAERYNLLNAVSEQLIGTALKEWAEIGLQPECRHARLSINLTPSQLGQ
SYWPRRLLSFCHKWQVDISRVTVEITENQALDKQSQHSGISRLRLHNFGVAIDDFGTGYTNLTQLCRLPISELKLDISLV
RGIHMDPLAQTILKSLLDIGRQLGVSVVAEGVEDPRDYRYLESVPGLIVQGFLVCRPKPLGELVRWLKARQRVAVDTKGL
EAGSSATYRH

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=249, Percent_Identity=30.9236947791165, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87082096, Length=253, Percent_Identity=32.0158102766798, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87081845, Length=264, Percent_Identity=30.3030303030303, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787541, Length=251, Percent_Identity=28.2868525896414, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788502, Length=244, Percent_Identity=28.6885245901639, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790496, Length=248, Percent_Identity=27.0161290322581, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787055, Length=254, Percent_Identity=27.9527559055118, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87081743, Length=244, Percent_Identity=27.0491803278689, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786507, Length=257, Percent_Identity=27.2373540856031, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788849, Length=212, Percent_Identity=31.1320754716981, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081980, Length=245, Percent_Identity=26.9387755102041, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510982, Length=244, Percent_Identity=29.5081967213115, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 46222; Mature: 46090

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNVLIIDDSLSYCQLLSKYFSELNVTSVDYCHDGSEALQRLRERRDRYQILVVDLHMP
CCEEEEEECCCHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHCCCEEEEEEEEECC
GMDGIELLSKLSGLEYRGGVIIASGMESRIIEAASQIVLGAHLRLLGALMKPVCKTQLNI
CCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CIERLLMMVPELNTSKPAMTLQSLRESIKQNKVVPYYQPQVEVGTGRIEGFEVLCRMQLG
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCC
AQLQLMTPDRFMALAERYNLLNAVSEQLIGTALKEWAEIGLQPECRHARLSINLTPSQLG
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHH
QSYWPRRLLSFCHKWQVDISRVTVEITENQALDKQSQHSGISRLRLHNFGVAIDDFGTGY
HHHHHHHHHHHHHHHCCEEEEEEEEEECCCHHCCHHHHCCHHHHHHHHCCEEEECCCCCH
TNLTQLCRLPISELKLDISLVRGIHMDPLAQTILKSLLDIGRQLGVSVVAEGVEDPRDYR
HHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
YLESVPGLIVQGFLVCRPKPLGELVRWLKARQRVAVDTKGLEAGSSATYRH
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLNVLIIDDSLSYCQLLSKYFSELNVTSVDYCHDGSEALQRLRERRDRYQILVVDLHMP
CEEEEEECCCHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHCCCEEEEEEEEECC
GMDGIELLSKLSGLEYRGGVIIASGMESRIIEAASQIVLGAHLRLLGALMKPVCKTQLNI
CCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CIERLLMMVPELNTSKPAMTLQSLRESIKQNKVVPYYQPQVEVGTGRIEGFEVLCRMQLG
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCC
AQLQLMTPDRFMALAERYNLLNAVSEQLIGTALKEWAEIGLQPECRHARLSINLTPSQLG
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHH
QSYWPRRLLSFCHKWQVDISRVTVEITENQALDKQSQHSGISRLRLHNFGVAIDDFGTGY
HHHHHHHHHHHHHHHCCEEEEEEEEEECCCHHCCHHHHCCHHHHHHHHCCEEEECCCCCH
TNLTQLCRLPISELKLDISLVRGIHMDPLAQTILKSLLDIGRQLGVSVVAEGVEDPRDYR
HHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
YLESVPGLIVQGFLVCRPKPLGELVRWLKARQRVAVDTKGLEAGSSATYRH
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]