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The map label for this gene is rumB

Identifier: 119773768

GI number: 119773768

Start: 782438

End: 783592

Strand: Direct

Name: rumB

Synonym: Sama_0630

Alternate gene names: 119773768

Gene position: 782438-783592 (Clockwise)

Preceding gene: 119773767

Following gene: 119773773

Centisome position: 18.17

GC content: 56.62

Gene sequence:

>1155_bases
GTGAGCTGTGACTATTTCAACCGGGGACTCTGTCAGTCGTGTCGTCTGATGTCCAAGCCAGTGACTGAGCAGTTGCTGGA
GAAAGAAGCCAGGCTCACCCACCTGCTGGGCGGTCTGCCGGTAGACGAACGTTTGGCTCCGGTATCCGGGCCCGAGTTTG
GTTTTCGCAACAAAGCAAAAATGGTGGTGATGGGCGCGGCCCACGCCCCTGTGCTGGGTATTCCGGGTCCCGATGGTCAA
CCCGTGGACCTCAGCCATTGCCCCCTCTATCCACAAGACATGCAGGCGCTCTTGCTTGAGCTGACTTCCTTTGTGCGCCG
TGCAGGTATTCCGCCTTACCGGGTCGATAAAGCCAAGGGCGAGCTTAAGTTTATTTTGCTGACCCGCAGCGCCGTGCGCG
GTGAGTTTATGCTGCGATTTGTGCTTCGAAGCAAAGATGCCATTCCCCGTATCGAACGTGAACTGCCGAAACTCTTGGCC
GATTTCCCGGCCATTAAGGTGGTCTCGGTGAACCTGCAACCGGTGCACATGGCAAGACTGGAAGGTGAAGAGGAAATCTT
TCTTACCCAAGCCACCCGCCTCGATGAGGTATTCAACGGGGTGCCACTCTTTATCCGCCCCAAAAGCTTTTTCCAAACCA
ACCCTGAGGTGGCCTCGCGTCTATACGCCACAGCCGCCAAGTGGGTTGATGAATTAAGTCCAAAGAGCCTGTGGGATCTT
TTTTGCGGTGTAGGCGGCTTTGGGTTGCACTGCGCCAATGAATCATTGCCGGTGACAGGCATTGAGATTGAAGCCGAAGC
CATCGACTGCGCCAAAACCTCGGCAGCGGCCATGGGACTGGATAATCTGTCGTTTGCTGCGCTGGATTCCACCGACTTTG
CCATGGGCCAGCAGGCACAGGAAGTACCCGAGGTCATCATAGTGAATCCACCGCGCCGGGGCATTGGTGAGGAACTGTGT
GAGCGTTTGTCGGCCTTTGGGCCGAGAGCCATCATTTATTCCAGCTGTAATCCCGAGACGCTGGCTAAAGATCTGGCCCT
GATCTCGGGCTATCGCATCGCCCGGGTACAGCTGTTCGACATGTTCCCCCACAGTGACCACTTTGAGGTGCTGTGTCTGC
TGCTCAAGGAAGCGGCCTGCGCCAGCGCAGACTGA

Upstream 100 bases:

>100_bases
GATGTGGATAAGCTGCCGCTGACCCGTCCGGTAAACCGGGATGATTTTATGGCGCAAACAGGCTAAAGTGTGCGCCGTTT
TTTAGATGTGGGAGCCGAAC

Downstream 100 bases:

>100_bases
TGGAGGCACCGCCAAGTTCGGCCGAGTTTTCCACCGTCAGTTTGCCCTGATGCCAGATGGCAATTTTGCGGACAATATAG
AGGCCCAGGCCAAATCCGGC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSCDYFNRGLCQSCRLMSKPVTEQLLEKEARLTHLLGGLPVDERLAPVSGPEFGFRNKAKMVVMGAAHAPVLGIPGPDGQ
PVDLSHCPLYPQDMQALLLELTSFVRRAGIPPYRVDKAKGELKFILLTRSAVRGEFMLRFVLRSKDAIPRIERELPKLLA
DFPAIKVVSVNLQPVHMARLEGEEEIFLTQATRLDEVFNGVPLFIRPKSFFQTNPEVASRLYATAAKWVDELSPKSLWDL
FCGVGGFGLHCANESLPVTGIEIEAEAIDCAKTSAAAMGLDNLSFAALDSTDFAMGQQAQEVPEVIIVNPPRRGIGEELC
ERLSAFGPRAIIYSSCNPETLAKDLALISGYRIARVQLFDMFPHSDHFEVLCLLLKEAACASAD

Sequences:

>Translated_384_residues
MSCDYFNRGLCQSCRLMSKPVTEQLLEKEARLTHLLGGLPVDERLAPVSGPEFGFRNKAKMVVMGAAHAPVLGIPGPDGQ
PVDLSHCPLYPQDMQALLLELTSFVRRAGIPPYRVDKAKGELKFILLTRSAVRGEFMLRFVLRSKDAIPRIERELPKLLA
DFPAIKVVSVNLQPVHMARLEGEEEIFLTQATRLDEVFNGVPLFIRPKSFFQTNPEVASRLYATAAKWVDELSPKSLWDL
FCGVGGFGLHCANESLPVTGIEIEAEAIDCAKTSAAAMGLDNLSFAALDSTDFAMGQQAQEVPEVIIVNPPRRGIGEELC
ERLSAFGPRAIIYSSCNPETLAKDLALISGYRIARVQLFDMFPHSDHFEVLCLLLKEAACASAD
>Mature_383_residues
SCDYFNRGLCQSCRLMSKPVTEQLLEKEARLTHLLGGLPVDERLAPVSGPEFGFRNKAKMVVMGAAHAPVLGIPGPDGQP
VDLSHCPLYPQDMQALLLELTSFVRRAGIPPYRVDKAKGELKFILLTRSAVRGEFMLRFVLRSKDAIPRIERELPKLLAD
FPAIKVVSVNLQPVHMARLEGEEEIFLTQATRLDEVFNGVPLFIRPKSFFQTNPEVASRLYATAAKWVDELSPKSLWDLF
CGVGGFGLHCANESLPVTGIEIEAEAIDCAKTSAAAMGLDNLSFAALDSTDFAMGQQAQEVPEVIIVNPPRRGIGEELCE
RLSAFGPRAIIYSSCNPETLAKDLALISGYRIARVQLFDMFPHSDHFEVLCLLLKEAACASAD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily

Homologues:

Organism=Homo sapiens, GI269784678, Length=406, Percent_Identity=23.6453201970443, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI34222389, Length=194, Percent_Identity=26.8041237113402, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI51173878, Length=194, Percent_Identity=26.8041237113402, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI269784680, Length=414, Percent_Identity=21.9806763285024, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI269784676, Length=414, Percent_Identity=21.9806763285024, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI20149688, Length=414, Percent_Identity=21.9806763285024, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787083, Length=377, Percent_Identity=57.0291777188329, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1789148, Length=379, Percent_Identity=26.9129287598945, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790403, Length=181, Percent_Identity=32.0441988950276, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666579, Length=361, Percent_Identity=25.207756232687, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMB_SHEAM (A1S382)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926508.1
- ProteinModelPortal:   A1S382
- SMR:   A1S382
- STRING:   A1S382
- GeneID:   4602883
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0630
- NMPDR:   fig|326297.7.peg.589
- eggNOG:   COG2265
- HOGENOM:   HBG296593
- OMA:   TMAKDIA
- PhylomeDB:   A1S382
- ProtClustDB:   PRK03522
- HAMAP:   MF_01012
- InterPro:   IPR011825
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02085

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.189

Molecular weight: Translated: 42244; Mature: 42113

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 336-336 BINDING 212-212 BINDING 241-241 BINDING 262-262 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCDYFNRGLCQSCRLMSKPVTEQLLEKEARLTHLLGGLPVDERLAPVSGPEFGFRNKAK
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCE
MVVMGAAHAPVLGIPGPDGQPVDLSHCPLYPQDMQALLLELTSFVRRAGIPPYRVDKAKG
EEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
ELKFILLTRSAVRGEFMLRFVLRSKDAIPRIERELPKLLADFPAIKVVSVNLQPVHMARL
CEEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHC
EGEEEIFLTQATRLDEVFNGVPLFIRPKSFFQTNPEVASRLYATAAKWVDELSPKSLWDL
CCCCEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCANESLPVTGIEIEAEAIDCAKTSAAAMGLDNLSFAALDSTDFAMGQQAQ
HHCCCCCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCHHH
EVPEVIIVNPPRRGIGEELCERLSAFGPRAIIYSSCNPETLAKDLALISGYRIARVQLFD
HCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEEEE
MFPHSDHFEVLCLLLKEAACASAD
CCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SCDYFNRGLCQSCRLMSKPVTEQLLEKEARLTHLLGGLPVDERLAPVSGPEFGFRNKAK
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCE
MVVMGAAHAPVLGIPGPDGQPVDLSHCPLYPQDMQALLLELTSFVRRAGIPPYRVDKAKG
EEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
ELKFILLTRSAVRGEFMLRFVLRSKDAIPRIERELPKLLADFPAIKVVSVNLQPVHMARL
CEEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHC
EGEEEIFLTQATRLDEVFNGVPLFIRPKSFFQTNPEVASRLYATAAKWVDELSPKSLWDL
CCCCEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCANESLPVTGIEIEAEAIDCAKTSAAAMGLDNLSFAALDSTDFAMGQQAQ
HHCCCCCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCHHH
EVPEVIIVNPPRRGIGEELCERLSAFGPRAIIYSSCNPETLAKDLALISGYRIARVQLFD
HCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEEEE
MFPHSDHFEVLCLLLKEAACASAD
CCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA