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The map label for this gene is ahpF [H]

Identifier: 119773756

GI number: 119773756

Start: 771262

End: 772824

Strand: Direct

Name: ahpF [H]

Synonym: Sama_0618

Alternate gene names: 119773756

Gene position: 771262-772824 (Clockwise)

Preceding gene: 119773755

Following gene: 119773757

Centisome position: 17.91

GC content: 55.15

Gene sequence:

>1563_bases
ATGTTAAACGCGGATTTGAAACAGCAACTTAAGACCTATCTGCAAAATCTACGGCAAGACGTCAGACTCGTGGTGTCTGC
CGATGACAGCAAAAAATCTCAGGAATTGCTCGACCTGGCCAATGACATCTCCTCCTTGTCTCCGTTGGTTTCAGTGACCC
AGGCCAGGCTCGAGCGCACCCCTGAAATGCAGGTAACCAATGCTCAGGGGACCGACATTCGTTTTGCCGGTTTGCCCATG
GGCCACGAATTTACGTCGTTGGTGTTGGCACTGCTGCACACAGGCGGTCATCCCCTCAAGCTGGATATGGCTGTTATTGA
ACAAATCAAGGCGCTGCCACAGACCCTGAAGTTTGAAACCTTTATCTCCCTGAGCTGCCAAAACTGTCCCGATGTGGTGC
AGGCTCTGAACATGATGGCCGCGCTGAATCCGAACGTCAGCAGCACCATGATTGATGGGGCCCTGTTTCAGGACGAGGTG
GAATCTCGGCAGATCATGGCCGTACCCTCGGTGTTCCTTAATGGTGAACTCTTTTCCCAGGGTCGCATCAGCCTGAAGGA
GATTCTCGCCAAGGTGGATACCCAGGCTGCAGCCCGAGAAGCCGATGCCCTGAGTGAGAAAGCCCCTTACGAAGTATTGG
TAGTGGGCGCAGGCCCCGCCGGTGCCTCTGCCGCCATTTATTCGGCCCGTAAAGGCTTGCGTACCGGTCTGGTTGCCGAC
CGATTTGGCGGTCAGGTGGCTGAAACCGTGGGCATTGAGAACTTTATTTCAGTGAAAGCCACTGAAGGCCCCAAGCTGGT
GGCAAACCTTGAAGCCCACGTTCGCGATTACGAAGTGGATGTGATGGAAAACCAGAAGGCTCTCAAGCTGACCAAAGACG
GTCTGTATCAGGTGGAGTTGGCCAATGGTGCTGTGTTATCGGGTAAAACTGTACTGATTGCCACCGGTGCACGCTGGCGT
GAAATGAATGTGCCCGGTGAAAAAGAATACCGTGGCCGCGGCGTGGCTTACTGTCCTCACTGTGATGGCCCCTTGTTCAA
AGGTAAACGCGTTGCCGTGATAGGTGGCGGTAATTCGGGTATCGAAGCTGCCATCGACCTGGCCAACATAGTTGAGCATG
TCACTGTGCTGGAGTTTGACAGCAAGCTGCGCGCCGATGAGGTGCTGGTTCGCAAGGCTCGCTCTATGGGGAATATCCAC
ATCATCACCCAGGCACAAACCACCGAGGTGGTGGGTGACGGCAACAAGGTGACCGGCTTGGTCTACACCGACCGAGCTAC
AGGTGATGTACACAGTGTTGAGCTGGCGGGTATCTTCGTACAGATTGGTCTTGTACCCAACTCAGAGTGGCTTAAGGGCA
CACTGGAACTGACCCCGCGCGGCGAGATCATAGTGGATGAGCGTGGCCAAACCTCGCTGCCGGGTGTATTTGCCGCGGGA
GACGTGACCAACTCTGCCTACAAGCAAATCATTATTGCCATGGGCAGTGGTGCTACCGCGAGCCTGGGGGCGTTTGATTA
CCTCATCCGTCACAGCGAGGCAGATGCTTCAGCTGCCGCCTGA

Upstream 100 bases:

>100_bases
TAAGCCTTAACCCGCATCACCCCTTGCTCCGGGCCTCCCCCTGATTTGGCCCGGAGCCTTTTCTCAGCGTTATTTATCTT
TGCTTGCATCTGGAGCCACC

Downstream 100 bases:

>100_bases
TAGCTCACGTCGCAAAAGCCGGCATTTTGCCGGCTTTTTTGTGTCTTCTTCATGCTTGCGTACACCTAGAATGGTGCGCT
GCACATGCTATAGTCATTCC

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MLNADLKQQLKTYLQNLRQDVRLVVSADDSKKSQELLDLANDISSLSPLVSVTQARLERTPEMQVTNAQGTDIRFAGLPM
GHEFTSLVLALLHTGGHPLKLDMAVIEQIKALPQTLKFETFISLSCQNCPDVVQALNMMAALNPNVSSTMIDGALFQDEV
ESRQIMAVPSVFLNGELFSQGRISLKEILAKVDTQAAAREADALSEKAPYEVLVVGAGPAGASAAIYSARKGLRTGLVAD
RFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDVMENQKALKLTKDGLYQVELANGAVLSGKTVLIATGARWR
EMNVPGEKEYRGRGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDSKLRADEVLVRKARSMGNIH
IITQAQTTEVVGDGNKVTGLVYTDRATGDVHSVELAGIFVQIGLVPNSEWLKGTLELTPRGEIIVDERGQTSLPGVFAAG
DVTNSAYKQIIIAMGSGATASLGAFDYLIRHSEADASAAA

Sequences:

>Translated_520_residues
MLNADLKQQLKTYLQNLRQDVRLVVSADDSKKSQELLDLANDISSLSPLVSVTQARLERTPEMQVTNAQGTDIRFAGLPM
GHEFTSLVLALLHTGGHPLKLDMAVIEQIKALPQTLKFETFISLSCQNCPDVVQALNMMAALNPNVSSTMIDGALFQDEV
ESRQIMAVPSVFLNGELFSQGRISLKEILAKVDTQAAAREADALSEKAPYEVLVVGAGPAGASAAIYSARKGLRTGLVAD
RFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDVMENQKALKLTKDGLYQVELANGAVLSGKTVLIATGARWR
EMNVPGEKEYRGRGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDSKLRADEVLVRKARSMGNIH
IITQAQTTEVVGDGNKVTGLVYTDRATGDVHSVELAGIFVQIGLVPNSEWLKGTLELTPRGEIIVDERGQTSLPGVFAAG
DVTNSAYKQIIIAMGSGATASLGAFDYLIRHSEADASAAA
>Mature_520_residues
MLNADLKQQLKTYLQNLRQDVRLVVSADDSKKSQELLDLANDISSLSPLVSVTQARLERTPEMQVTNAQGTDIRFAGLPM
GHEFTSLVLALLHTGGHPLKLDMAVIEQIKALPQTLKFETFISLSCQNCPDVVQALNMMAALNPNVSSTMIDGALFQDEV
ESRQIMAVPSVFLNGELFSQGRISLKEILAKVDTQAAAREADALSEKAPYEVLVVGAGPAGASAAIYSARKGLRTGLVAD
RFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDVMENQKALKLTKDGLYQVELANGAVLSGKTVLIATGARWR
EMNVPGEKEYRGRGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDSKLRADEVLVRKARSMGNIH
IITQAQTTEVVGDGNKVTGLVYTDRATGDVHSVELAGIFVQIGLVPNSEWLKGTLELTPRGEIIVDERGQTSLPGVFAAG
DVTNSAYKQIIIAMGSGATASLGAFDYLIRHSEADASAAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=523, Percent_Identity=57.9349904397706, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1787114, Length=309, Percent_Identity=35.5987055016181, Blast_Score=174, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6321898, Length=337, Percent_Identity=30.2670623145401, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320560, Length=311, Percent_Identity=31.1897106109325, Blast_Score=137, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 55607; Mature: 55607

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNADLKQQLKTYLQNLRQDVRLVVSADDSKKSQELLDLANDISSLSPLVSVTQARLERT
CCCHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEMQVTNAQGTDIRFAGLPMGHEFTSLVLALLHTGGHPLKLDMAVIEQIKALPQTLKFET
CCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHEEEE
FISLSCQNCPDVVQALNMMAALNPNVSSTMIDGALFQDEVESRQIMAVPSVFLNGELFSQ
EEEEECCCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCEEEEECCEEECCCCCCC
GRISLKEILAKVDTQAAAREADALSEKAPYEVLVVGAGPAGASAAIYSARKGLRTGLVAD
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHH
RFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDVMENQKALKLTKDGLYQVEL
HCCCHHHHHHCCCCEEEEEECCCCEEEEEHHHHHEEEEEEEECCCCEEEEECCCEEEEEE
ANGAVLSGKTVLIATGARWREMNVPGEKEYRGRGVAYCPHCDGPLFKGKRVAVIGGGNSG
CCCEEECCCEEEEECCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCC
IEAAIDLANIVEHVTVLEFDSKLRADEVLVRKARSMGNIHIITQAQTTEVVGDGNKVTGL
HHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCEEEEECCCCEEEEE
VYTDRATGDVHSVELAGIFVQIGLVPNSEWLKGTLELTPRGEIIVDERGQTSLPGVFAAG
EEECCCCCCEEEEEEEEEEEEEEECCCHHHCEEEEEECCCCCEEEECCCCCCCCCEEEEC
DVTNSAYKQIIIAMGSGATASLGAFDYLIRHSEADASAAA
CCCHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLNADLKQQLKTYLQNLRQDVRLVVSADDSKKSQELLDLANDISSLSPLVSVTQARLERT
CCCHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEMQVTNAQGTDIRFAGLPMGHEFTSLVLALLHTGGHPLKLDMAVIEQIKALPQTLKFET
CCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHEEEE
FISLSCQNCPDVVQALNMMAALNPNVSSTMIDGALFQDEVESRQIMAVPSVFLNGELFSQ
EEEEECCCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCEEEEECCEEECCCCCCC
GRISLKEILAKVDTQAAAREADALSEKAPYEVLVVGAGPAGASAAIYSARKGLRTGLVAD
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHH
RFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDVMENQKALKLTKDGLYQVEL
HCCCHHHHHHCCCCEEEEEECCCCEEEEEHHHHHEEEEEEEECCCCEEEEECCCEEEEEE
ANGAVLSGKTVLIATGARWREMNVPGEKEYRGRGVAYCPHCDGPLFKGKRVAVIGGGNSG
CCCEEECCCEEEEECCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCC
IEAAIDLANIVEHVTVLEFDSKLRADEVLVRKARSMGNIHIITQAQTTEVVGDGNKVTGL
HHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCEEEEECCCCEEEEE
VYTDRATGDVHSVELAGIFVQIGLVPNSEWLKGTLELTPRGEIIVDERGQTSLPGVFAAG
EEECCCCCCEEEEEEEEEEEEEEECCCHHHCEEEEEECCCCCEEEECCCCCCCCCEEEEC
DVTNSAYKQIIIAMGSGATASLGAFDYLIRHSEADASAAA
CCCHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]