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The map label for this gene is ldhA [H]

Identifier: 119773750

GI number: 119773750

Start: 765389

End: 766378

Strand: Direct

Name: ldhA [H]

Synonym: Sama_0612

Alternate gene names: 119773750

Gene position: 765389-766378 (Clockwise)

Preceding gene: 119773749

Following gene: 119773751

Centisome position: 17.77

GC content: 52.93

Gene sequence:

>990_bases
ATGAAAATCGGATTCTTCAGCGCCAAACACTACGACATGCAGCACTTTGACCGCACCAATGCCAGTTTCGGTGCTCATAT
TGAATATTTTGATTCCAGACTGTGCATGCAAACCGTCAAGCTGGCTTATGGCTTTGAAGTGATTTGCGCCTTTGTAAACG
ACGAGCTGTCGGCCGAAGTCCTGAATGAACTCCATGGCAACGGTACCCGCGTCATAGCCATGCGCTGTGCCGGTTTCAAC
AATGTGGATTTGGAAGAGGCCAAACACCTTGGTATGACGGTAGTCAACGTACCCGCTTATTCGCCGGAGTCAGTCGCCGA
GCACACAGTGGCCCTGATGTTGACCCTGAACCGCAAAATTCACAAAGCTTACCAGCGTACCCGGGATGCCAATTTCTCTC
TGGAAGGGCTGGTGGGATTTAACATGCACGGCAAAACCGTCGGTGTCATAGGGACCGGTAAAATTGGCCTTGCGACTATT
CGTATCCTGCTGGGCTTTGGTTGCAAGGTGGTGGCTTTTGACCCATTCCCCAGCCCTGCGGTAGAAGCCCTTGGGGTGCC
CTATCTGGAACTGGATGAATTGCTGAAGCTGTGTGACGTAGTGAGCTTGCACTGTCCGTTGACCAAGGAAAACCATCACC
TGCTGCGCGCCGAGACCTTTGCCAAAATGAAGCCCGGTGTCATGGTCATCAACACCAGCCGTGGTGGCCTGTTGAACGCC
TTTGATGCCATGGAAGCGCTGAAGGTCGGCCAGATTGGCGCCCTCGGGCTCGACGTGTATGAAAACGAAAAAGAGCTGTT
TTTCGAAGACAAGTCCAACGAAGTGATTCAAGACGATGTATTCCGCCGTCTGTCGGCTTGCCACAACGTGGTCTTTACCG
GTCATCAGGCGTTTTTGACCGAAGAAGCGCTTGGCGCCATTGCGACTACGACCCTGACCAATGTGCAAAAAGCCCTGGCC
GGTGAGCGCTGCGGTAACGAACTTTTCTGA

Upstream 100 bases:

>100_bases
ATTTTAAGTAAAGGTGATCCACATCACCCTTTGCGCAACGACAGTCGGCTTATTTTGGCTTACACTGCGGCCATTCAGGC
TTTTTCCAGGAACGCCCACC

Downstream 100 bases:

>100_bases
TACGCGGCGCTGACAGCGGCAACGGGGCTTGCTAGATTGAGTGCAGTTTCTCTTTCAGGAGGTCCCGATGCGCGTCTGTG
CCGAACACCATGTTATCAAT

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKIGFFSAKHYDMQHFDRTNASFGAHIEYFDSRLCMQTVKLAYGFEVICAFVNDELSAEVLNELHGNGTRVIAMRCAGFN
NVDLEEAKHLGMTVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLEGLVGFNMHGKTVGVIGTGKIGLATI
RILLGFGCKVVAFDPFPSPAVEALGVPYLELDELLKLCDVVSLHCPLTKENHHLLRAETFAKMKPGVMVINTSRGGLLNA
FDAMEALKVGQIGALGLDVYENEKELFFEDKSNEVIQDDVFRRLSACHNVVFTGHQAFLTEEALGAIATTTLTNVQKALA
GERCGNELF

Sequences:

>Translated_329_residues
MKIGFFSAKHYDMQHFDRTNASFGAHIEYFDSRLCMQTVKLAYGFEVICAFVNDELSAEVLNELHGNGTRVIAMRCAGFN
NVDLEEAKHLGMTVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLEGLVGFNMHGKTVGVIGTGKIGLATI
RILLGFGCKVVAFDPFPSPAVEALGVPYLELDELLKLCDVVSLHCPLTKENHHLLRAETFAKMKPGVMVINTSRGGLLNA
FDAMEALKVGQIGALGLDVYENEKELFFEDKSNEVIQDDVFRRLSACHNVVFTGHQAFLTEEALGAIATTTLTNVQKALA
GERCGNELF
>Mature_329_residues
MKIGFFSAKHYDMQHFDRTNASFGAHIEYFDSRLCMQTVKLAYGFEVICAFVNDELSAEVLNELHGNGTRVIAMRCAGFN
NVDLEEAKHLGMTVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLEGLVGFNMHGKTVGVIGTGKIGLATI
RILLGFGCKVVAFDPFPSPAVEALGVPYLELDELLKLCDVVSLHCPLTKENHHLLRAETFAKMKPGVMVINTSRGGLLNA
FDAMEALKVGQIGALGLDVYENEKELFFEDKSNEVIQDDVFRRLSACHNVVFTGHQAFLTEEALGAIATTTLTNVQKALA
GERCGNELF

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=210, Percent_Identity=35.2380952380952, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI61743967, Length=262, Percent_Identity=32.824427480916, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4557497, Length=262, Percent_Identity=32.824427480916, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI145580578, Length=261, Percent_Identity=32.183908045977, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4557499, Length=261, Percent_Identity=32.183908045977, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI145580575, Length=262, Percent_Identity=32.0610687022901, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI6912396, Length=248, Percent_Identity=29.4354838709677, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=61.890243902439, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI87082289, Length=275, Percent_Identity=29.4545454545455, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789279, Length=229, Percent_Identity=29.2576419213974, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=216, Percent_Identity=31.9444444444444, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25147481, Length=260, Percent_Identity=30.3846153846154, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=199, Percent_Identity=34.1708542713568, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=301, Percent_Identity=30.2325581395349, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=248, Percent_Identity=32.258064516129, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=28.4313725490196, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=27.1428571428571, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646446, Length=261, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646448, Length=261, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646452, Length=261, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646450, Length=261, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI62472511, Length=261, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19921140, Length=289, Percent_Identity=28.0276816608997, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28574286, Length=275, Percent_Identity=30.9090909090909, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45552429, Length=275, Percent_Identity=29.8181818181818, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585514, Length=275, Percent_Identity=29.8181818181818, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28574282, Length=275, Percent_Identity=29.8181818181818, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28574284, Length=275, Percent_Identity=29.8181818181818, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI45551003, Length=275, Percent_Identity=29.8181818181818, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI28571528, Length=300, Percent_Identity=27.3333333333333, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585516, Length=202, Percent_Identity=24.2574257425743, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36190; Mature: 36190

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGFFSAKHYDMQHFDRTNASFGAHIEYFDSRLCMQTVKLAYGFEVICAFVNDELSAEV
CEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LNELHGNGTRVIAMRCAGFNNVDLEEAKHLGMTVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHCCCCEEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
HKAYQRTRDANFSLEGLVGFNMHGKTVGVIGTGKIGLATIRILLGFGCKVVAFDPFPSPA
HHHHHHHCCCCCEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHH
VEALGVPYLELDELLKLCDVVSLHCPLTKENHHLLRAETFAKMKPGVMVINTSRGGLLNA
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHCCCCEEEEECCCCCCHHH
FDAMEALKVGQIGALGLDVYENEKELFFEDKSNEVIQDDVFRRLSACHNVVFTGHQAFLT
HHHHHHHHCCCCCEEECEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEECCHHHHH
EEALGAIATTTLTNVQKALAGERCGNELF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKIGFFSAKHYDMQHFDRTNASFGAHIEYFDSRLCMQTVKLAYGFEVICAFVNDELSAEV
CEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LNELHGNGTRVIAMRCAGFNNVDLEEAKHLGMTVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHCCCCEEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
HKAYQRTRDANFSLEGLVGFNMHGKTVGVIGTGKIGLATIRILLGFGCKVVAFDPFPSPA
HHHHHHHCCCCCEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHH
VEALGVPYLELDELLKLCDVVSLHCPLTKENHHLLRAETFAKMKPGVMVINTSRGGLLNA
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHCCCCEEEEECCCCCCHHH
FDAMEALKVGQIGALGLDVYENEKELFFEDKSNEVIQDDVFRRLSACHNVVFTGHQAFLT
HHHHHHHHCCCCCEEECEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEECCHHHHH
EEALGAIATTTLTNVQKALAGERCGNELF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]