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The map label for this gene is phaZ1 [H]

Identifier: 119773660

GI number: 119773660

Start: 639484

End: 640431

Strand: Direct

Name: phaZ1 [H]

Synonym: Sama_0520

Alternate gene names: 119773660

Gene position: 639484-640431 (Clockwise)

Preceding gene: 119773659

Following gene: 119773661

Centisome position: 14.85

GC content: 52.53

Gene sequence:

>948_bases
ATGTTCAAAGTCTCTTCGTTGGCGCTCAAGGCTGCGACCATTTTAGCAAGTAGCCTGTCACTCACTGTTGCCGCCCATGC
TTCAGCACGGGCCTTCGAGCCGGTTAACGACTTTGGTGCCAATCCCGGAGCATTAACGGCGTCAATCTACACGCCTGCAA
CCAACCCCGGCAGTGCTCTGGTGGTGCTGCTTCACGGCTGCGTGCAGGATGGTATCGAGCTTGCCGATAACAGCGGGTTA
ACTGGCCTTGCCCAAGAGAAGCAATTTGCCGTGCTTGTGCCTCAGCAGAGTTTTGATAATAACGTAAAACGCTGTTTTAA
CTGGTTTTCAGCTCAGGATACCCAGGTTGACAGCGGCGAAATGCTATCGCTTAAAAACATGATTACTCAGGCACAGGCCA
CTACTGGCGCCAAACGTGTGTATCTGGTGGGCCTATCTGCCGGCGGCGCAATGGCCAGTGCGGCACTTGTTAATTATCCC
GACTTTTTCACTGCCGGTGCTGTGATAGCAGGTCTGCCTTATCCCTGTGCCGATAACCTGACCAAAGCCATTTCCTGTAT
GAAAAAAGGGCCGGCGGAATCCACAGGGGAACTTGTGAGCTTTGTGCGCAAGTTACATCCAGATCAAGCCTCCTGGCCGA
CACTCAGCATCTGGACAGGCACAACAGATGCGGTGGTGCACCCACAGAATGCCCGGAGTTTGGCGGCGCAGTGGCTGCAG
TTATCCGGTGTAGAGGCAGCACCCAAGGTTGAAGAATTTACCGATTATCGGATCAGTCGCTGGACCGATACCAAGGGGCA
GCCCTTAGTGGATTTGGTGGAGATTAATAACATGGGCCATGGTATTTCGGTGAATCCGGCAATAACTAATGGCGGAAAAG
AGGGGGACTTTTTATTGAAGGCCCCAATCAGCAGCATGGATGAAATTATTCGGTCTTGGGGATTATAG

Upstream 100 bases:

>100_bases
CTGTCTCAGGGGCGACAGTCTGGTTCTAAAGTGCCATTGCCAGCCGGGTGCAAATTACGCAAGTTTGCAGTGTCTGTATT
TCTCCGTTGGAGCCTGTTTT

Downstream 100 bases:

>100_bases
ACGGATTGAAATAAATAATTTCCAGATGGGTTGTTTGTCCGGTTTATAAATCTCTTTTTAAGTGGTTTTTAATTCGATAT
GATTTTGAAATTTTATCGAT

Product: putative polyhydroxyalkanoate depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MFKVSSLALKAATILASSLSLTVAAHASARAFEPVNDFGANPGALTASIYTPATNPGSALVVLLHGCVQDGIELADNSGL
TGLAQEKQFAVLVPQQSFDNNVKRCFNWFSAQDTQVDSGEMLSLKNMITQAQATTGAKRVYLVGLSAGGAMASAALVNYP
DFFTAGAVIAGLPYPCADNLTKAISCMKKGPAESTGELVSFVRKLHPDQASWPTLSIWTGTTDAVVHPQNARSLAAQWLQ
LSGVEAAPKVEEFTDYRISRWTDTKGQPLVDLVEINNMGHGISVNPAITNGGKEGDFLLKAPISSMDEIIRSWGL

Sequences:

>Translated_315_residues
MFKVSSLALKAATILASSLSLTVAAHASARAFEPVNDFGANPGALTASIYTPATNPGSALVVLLHGCVQDGIELADNSGL
TGLAQEKQFAVLVPQQSFDNNVKRCFNWFSAQDTQVDSGEMLSLKNMITQAQATTGAKRVYLVGLSAGGAMASAALVNYP
DFFTAGAVIAGLPYPCADNLTKAISCMKKGPAESTGELVSFVRKLHPDQASWPTLSIWTGTTDAVVHPQNARSLAAQWLQ
LSGVEAAPKVEEFTDYRISRWTDTKGQPLVDLVEINNMGHGISVNPAITNGGKEGDFLLKAPISSMDEIIRSWGL
>Mature_315_residues
MFKVSSLALKAATILASSLSLTVAAHASARAFEPVNDFGANPGALTASIYTPATNPGSALVVLLHGCVQDGIELADNSGL
TGLAQEKQFAVLVPQQSFDNNVKRCFNWFSAQDTQVDSGEMLSLKNMITQAQATTGAKRVYLVGLSAGGAMASAALVNYP
DFFTAGAVIAGLPYPCADNLTKAISCMKKGPAESTGELVSFVRKLHPDQASWPTLSIWTGTTDAVVHPQNARSLAAQWLQ
LSGVEAAPKVEEFTDYRISRWTDTKGQPLVDLVEINNMGHGISVNPAITNGGKEGDFLLKAPISSMDEIIRSWGL

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: 3.1.1.-

Molecular weight: Translated: 33300; Mature: 33300

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVSSLALKAATILASSLSLTVAAHASARAFEPVNDFGANPGALTASIYTPATNPGSAL
CCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHH
VVLLHGCVQDGIELADNSGLTGLAQEKQFAVLVPQQSFDNNVKRCFNWFSAQDTQVDSGE
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCC
MLSLKNMITQAQATTGAKRVYLVGLSAGGAMASAALVNYPDFFTAGAVIAGLPYPCADNL
HHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHH
TKAISCMKKGPAESTGELVSFVRKLHPDQASWPTLSIWTGTTDAVVHPQNARSLAAQWLQ
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECCCCHHHHHHHHHH
LSGVEAAPKVEEFTDYRISRWTDTKGQPLVDLVEINNMGHGISVNPAITNGGKEGDFLLK
HCCCCCCCCHHHHHCCHHEECCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCEEEE
APISSMDEIIRSWGL
CCHHHHHHHHHHHCC
>Mature Secondary Structure
MFKVSSLALKAATILASSLSLTVAAHASARAFEPVNDFGANPGALTASIYTPATNPGSAL
CCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHH
VVLLHGCVQDGIELADNSGLTGLAQEKQFAVLVPQQSFDNNVKRCFNWFSAQDTQVDSGE
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCC
MLSLKNMITQAQATTGAKRVYLVGLSAGGAMASAALVNYPDFFTAGAVIAGLPYPCADNL
HHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHH
TKAISCMKKGPAESTGELVSFVRKLHPDQASWPTLSIWTGTTDAVVHPQNARSLAAQWLQ
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECCCCHHHHHHHHHH
LSGVEAAPKVEEFTDYRISRWTDTKGQPLVDLVEINNMGHGISVNPAITNGGKEGDFLLK
HCCCCCCCCHHHHHCCHHEECCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCEEEE
APISSMDEIIRSWGL
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]