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The map label for this gene is lpsJ [H]

Identifier: 119773659

GI number: 119773659

Start: 638675

End: 639340

Strand: Direct

Name: lpsJ [H]

Synonym: Sama_0519

Alternate gene names: 119773659

Gene position: 638675-639340 (Clockwise)

Preceding gene: 119773658

Following gene: 119773660

Centisome position: 14.83

GC content: 56.46

Gene sequence:

>666_bases
ATGGCACTTTCCAGAGAACAACTCGCCCAGCGTGTCGCACAGGAACTCAAGGACGGCTATTACGTCAACCTCGGCATAGG
CATTCCCACCCTGGTGGCTAACTACATTCCCGAGGGGATAGAAGTCATGCTGCAGTCGGAAAACGGCTTGCTGGGCATGG
GACCTTTCCCCACTGAGGATGAAGTCGACCCCGACCTTATCAATGCGGGCAAGCAAACCGTGACCATGGCGACCGGTGCG
GCCCTGTTTGACTCCGCCGAGTCATTCGCCATGATCCGTGGCGGCCATGTGGACTTAACCGTGCTCGGCGCCTTCGAAGT
GGATACCCAGGGCAATATCGCCTCCTACATGATCCCGGGGAAACTCATCAAGGGGATGGGCGGTGCCATGGATTTGGTGG
CGGGCGCCGATAACATCATAGTGACCATGACCCACGCCTCGAAACAGGGTGAATCCAAGCTGCTTACCCAATGCAGCCTG
CCGCTGACCGGTAAAGGCTGTATCAAGAAGGTGCTAACCGATCTTGCCTTTATCGAAATCAAAGACGGGAAATTCCATCT
GCTTGAGCGGGCCCCCGGTGTGAGTGTGGAAGAGATTATCAAGCTGACCGCCGGAGAACTGGTGGTGCCTGAGCATGTGC
CTGAGATGTGCTTTCAGAGCGCGTAA

Upstream 100 bases:

>100_bases
CCCAGGCATTTACGTCGACAGGCTGATCCAGGGCAGTTTCGAGAAGCGCATCGAGCAGCTCACCATCCGTCCCTCTTCAC
ACGGTAAATAGGAGTTCACC

Downstream 100 bases:

>100_bases
GCCTGAAAATCGGTTGTCAGCTTCAGAGTCTGCGTCCAAGAGCCTGTCTCAGGGGCGACAGTCTGGTTCTAAAGTGCCAT
TGCCAGCCGGGTGCAAATTA

Product: 3-oxoacid CoA-transferase

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MALSREQLAQRVAQELKDGYYVNLGIGIPTLVANYIPEGIEVMLQSENGLLGMGPFPTEDEVDPDLINAGKQTVTMATGA
ALFDSAESFAMIRGGHVDLTVLGAFEVDTQGNIASYMIPGKLIKGMGGAMDLVAGADNIIVTMTHASKQGESKLLTQCSL
PLTGKGCIKKVLTDLAFIEIKDGKFHLLERAPGVSVEEIIKLTAGELVVPEHVPEMCFQSA

Sequences:

>Translated_221_residues
MALSREQLAQRVAQELKDGYYVNLGIGIPTLVANYIPEGIEVMLQSENGLLGMGPFPTEDEVDPDLINAGKQTVTMATGA
ALFDSAESFAMIRGGHVDLTVLGAFEVDTQGNIASYMIPGKLIKGMGGAMDLVAGADNIIVTMTHASKQGESKLLTQCSL
PLTGKGCIKKVLTDLAFIEIKDGKFHLLERAPGVSVEEIIKLTAGELVVPEHVPEMCFQSA
>Mature_220_residues
ALSREQLAQRVAQELKDGYYVNLGIGIPTLVANYIPEGIEVMLQSENGLLGMGPFPTEDEVDPDLINAGKQTVTMATGAA
LFDSAESFAMIRGGHVDLTVLGAFEVDTQGNIASYMIPGKLIKGMGGAMDLVAGADNIIVTMTHASKQGESKLLTQCSLP
LTGKGCIKKVLTDLAFIEIKDGKFHLLERAPGVSVEEIIKLTAGELVVPEHVPEMCFQSA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=213, Percent_Identity=51.1737089201878, Blast_Score=224, Evalue=3e-59,
Organism=Homo sapiens, GI11545841, Length=215, Percent_Identity=49.3023255813954, Blast_Score=217, Evalue=7e-57,
Organism=Escherichia coli, GI1788552, Length=196, Percent_Identity=51.0204081632653, Blast_Score=217, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17531517, Length=216, Percent_Identity=53.2407407407407, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=56.0747663551402, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=56.0747663551402, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23492; Mature: 23360

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSREQLAQRVAQELKDGYYVNLGIGIPTLVANYIPEGIEVMLQSENGLLGMGPFPTED
CCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
EVDPDLINAGKQTVTMATGAALFDSAESFAMIRGGHVDLTVLGAFEVDTQGNIASYMIPG
CCCHHHHHCCCEEEEEECCHHHHHCCHHEEEEECCCEEEEEEEEEEECCCCCEEEEECCH
KLIKGMGGAMDLVAGADNIIVTMTHASKQGESKLLTQCSLPLTGKGCIKKVLTDLAFIEI
HHHHCCCCHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHEEEEE
KDGKFHLLERAPGVSVEEIIKLTAGELVVPEHVPEMCFQSA
CCCCEEEEECCCCCCHHHHHHHHCCCEECCHHHHHHHHCCC
>Mature Secondary Structure 
ALSREQLAQRVAQELKDGYYVNLGIGIPTLVANYIPEGIEVMLQSENGLLGMGPFPTED
CCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
EVDPDLINAGKQTVTMATGAALFDSAESFAMIRGGHVDLTVLGAFEVDTQGNIASYMIPG
CCCHHHHHCCCEEEEEECCHHHHHCCHHEEEEECCCEEEEEEEEEEECCCCCEEEEECCH
KLIKGMGGAMDLVAGADNIIVTMTHASKQGESKLLTQCSLPLTGKGCIKKVLTDLAFIEI
HHHHCCCCHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHEEEEE
KDGKFHLLERAPGVSVEEIIKLTAGELVVPEHVPEMCFQSA
CCCCEEEEECCCCCCHHHHHHHHCCCEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]