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The map label for this gene is pmbA [H]

Identifier: 119773622

GI number: 119773622

Start: 592968

End: 594329

Strand: Direct

Name: pmbA [H]

Synonym: Sama_0482

Alternate gene names: 119773622

Gene position: 592968-594329 (Clockwise)

Preceding gene: 119773620

Following gene: 119773624

Centisome position: 13.77

GC content: 55.73

Gene sequence:

>1362_bases
GTGAACAAGAGCAAGACAGTGTCATCTAACAAAATTGAATTGGAATTGAACGCCCTCAAGGATGCCGTGGCCATGGCGCT
GGATTACGCCAACACCCTGGGAACAGGCGCCGCCGAAGTGGCTATCAGCAAGCAACAGGGATTGTCGGTTTCTACCCGTC
TCAAGGAAGTGGAAACCGTTGAATTCAATAAAGACGGTGCACTGGGGATCACGGTATTCCGCGATGGCTGCAAAGGCAGT
TCATCCACCTCGGATTTAAGCCCCGAGGCTATCCGGCAGGCAGTAAAGGCCGCCGATGATATTGCCCGCTATACCTCCGC
CGACCCCTGTAACGGTTTGGCAGAAGCCAGTTTGATGGCTAAAACCGTGGCTGATTTGGATCTCTATCATCCCGAGAGCC
TGACACCGGAAGAGCTGACCGAACTGGCCATTCGCGCAGAGGCGGCAGCCCTTGACGCCGACCCGCGCATTAAAAATTCC
GATGGCGCCAGCGCCAACGCCCATACCGGCATCAAGGTGTATGGCAACAGCCATGGCTTCCTCAATGGCTATTGCTCCTC
ACGTTACTCCTTAAGCTGCGTGGTGATTGGGGAAGAGGACGATGGCAACATGCAGCGGGACTACGATTACAGCATTTCCC
GCAAATATGCCGAGCTGCTCTCGCCCGAGCTGGTGGGGCAGAAGGCTGCAGCCAAGACGTTAAGCCGCCTCGGTGGCCGC
AAAATTGCCACAGGTACCATGCCAGTGCTCTTTGCCCACGAAGTGGCAACCGGCCTGATTGGTCATCTTATCAGTGCCAT
CAGTGGCTCCAGCCTGTATCGCAAATCAAGCTTCCTGTTGGATGGGCTGGACACCCAGGTGTTTCCCGAGTGGTTGAATA
TCCGCGAAGAGCCACACCTGCTGGCAGGTCTTGCCAGTGCCCCATACGACAGTGAAGGTGTGGCGACTGTGGACAGAGAC
ATCATCAAGGACGGATTGCTCGCCACCTACCTGCTGACCTCCTATTCGGCCCGTAAACTGGGGATGACCAATACAGGCCA
TGCCGGCGGTATTTACAACTGGACCCTGAATGACACTGGTGTGGATTTCGATGCGCTGGTACGCCAGATGGACAAGGGGC
TGATTGTGACTGAGGTAATGGGGCAGGGGGTGAACATGGTGACGGGTGACTACTCCCGTGGCGCCGCCGGATTCTATGTG
GAGAACGGTGAAATCCAGTACCCGGTGGAAGAAATTACCATCGCCGGTAACCTCAAAGACATGTATCGCAGTATTCAGGC
GGTGAGTCAGGACAGAGACCTGCGCTCTTCCATCCGTACCGGTGGTATTTTGCTGGATAGCATGAAGGTCGCCGGCAACT
GA

Upstream 100 bases:

>100_bases
GGGGAAACTGCTGCCTCGGGCGCAGATTTTGTCTGCAAAGGGAGCACATTTGTGGATGTGATTTGCCACAGGGCTTTGTT
ATGCTTAGCCCCATCAGGCA

Downstream 100 bases:

>100_bases
GGCTTTTAAGGCTAAGTGGTTTGTCGATGAAGCCCCGTTGATACGGGGCTTTTTATTGGCAGATATGTGGCGCCGGGAAA
CGGCAATAGCAAGAAGAGGG

Product: PmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MNKSKTVSSNKIELELNALKDAVAMALDYANTLGTGAAEVAISKQQGLSVSTRLKEVETVEFNKDGALGITVFRDGCKGS
SSTSDLSPEAIRQAVKAADDIARYTSADPCNGLAEASLMAKTVADLDLYHPESLTPEELTELAIRAEAAALDADPRIKNS
DGASANAHTGIKVYGNSHGFLNGYCSSRYSLSCVVIGEEDDGNMQRDYDYSISRKYAELLSPELVGQKAAAKTLSRLGGR
KIATGTMPVLFAHEVATGLIGHLISAISGSSLYRKSSFLLDGLDTQVFPEWLNIREEPHLLAGLASAPYDSEGVATVDRD
IIKDGLLATYLLTSYSARKLGMTNTGHAGGIYNWTLNDTGVDFDALVRQMDKGLIVTEVMGQGVNMVTGDYSRGAAGFYV
ENGEIQYPVEEITIAGNLKDMYRSIQAVSQDRDLRSSIRTGGILLDSMKVAGN

Sequences:

>Translated_453_residues
MNKSKTVSSNKIELELNALKDAVAMALDYANTLGTGAAEVAISKQQGLSVSTRLKEVETVEFNKDGALGITVFRDGCKGS
SSTSDLSPEAIRQAVKAADDIARYTSADPCNGLAEASLMAKTVADLDLYHPESLTPEELTELAIRAEAAALDADPRIKNS
DGASANAHTGIKVYGNSHGFLNGYCSSRYSLSCVVIGEEDDGNMQRDYDYSISRKYAELLSPELVGQKAAAKTLSRLGGR
KIATGTMPVLFAHEVATGLIGHLISAISGSSLYRKSSFLLDGLDTQVFPEWLNIREEPHLLAGLASAPYDSEGVATVDRD
IIKDGLLATYLLTSYSARKLGMTNTGHAGGIYNWTLNDTGVDFDALVRQMDKGLIVTEVMGQGVNMVTGDYSRGAAGFYV
ENGEIQYPVEEITIAGNLKDMYRSIQAVSQDRDLRSSIRTGGILLDSMKVAGN
>Mature_453_residues
MNKSKTVSSNKIELELNALKDAVAMALDYANTLGTGAAEVAISKQQGLSVSTRLKEVETVEFNKDGALGITVFRDGCKGS
SSTSDLSPEAIRQAVKAADDIARYTSADPCNGLAEASLMAKTVADLDLYHPESLTPEELTELAIRAEAAALDADPRIKNS
DGASANAHTGIKVYGNSHGFLNGYCSSRYSLSCVVIGEEDDGNMQRDYDYSISRKYAELLSPELVGQKAAAKTLSRLGGR
KIATGTMPVLFAHEVATGLIGHLISAISGSSLYRKSSFLLDGLDTQVFPEWLNIREEPHLLAGLASAPYDSEGVATVDRD
IIKDGLLATYLLTSYSARKLGMTNTGHAGGIYNWTLNDTGVDFDALVRQMDKGLIVTEVMGQGVNMVTGDYSRGAAGFYV
ENGEIQYPVEEITIAGNLKDMYRSIQAVSQDRDLRSSIRTGGILLDSMKVAGN

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=444, Percent_Identity=59.6846846846847, Blast_Score=549, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48439; Mature: 48439

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSKTVSSNKIELELNALKDAVAMALDYANTLGTGAAEVAISKQQGLSVSTRLKEVETV
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHCCCCHHHHHHHHEEE
EFNKDGALGITVFRDGCKGSSSTSDLSPEAIRQAVKAADDIARYTSADPCNGLAEASLMA
EECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KTVADLDLYHPESLTPEELTELAIRAEAAALDADPRIKNSDGASANAHTGIKVYGNSHGF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCC
LNGYCSSRYSLSCVVIGEEDDGNMQRDYDYSISRKYAELLSPELVGQKAAAKTLSRLGGR
EEEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCHHHHCHHHHHHHHHHHCCC
KIATGTMPVLFAHEVATGLIGHLISAISGSSLYRKSSFLLDGLDTQVFPEWLNIREEPHL
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHH
LAGLASAPYDSEGVATVDRDIIKDGLLATYLLTSYSARKLGMTNTGHAGGIYNWTLNDTG
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEECCCCC
VDFDALVRQMDKGLIVTEVMGQGVNMVTGDYSRGAAGFYVENGEIQYPVEEITIAGNLKD
CCHHHHHHHHHCCEEEEHHHCCCCEEEECCCCCCCCEEEEECCEEECCHHHEEEECCHHH
MYRSIQAVSQDRDLRSSIRTGGILLDSMKVAGN
HHHHHHHHHHHHHHHHHHHCCCEEEECEECCCC
>Mature Secondary Structure
MNKSKTVSSNKIELELNALKDAVAMALDYANTLGTGAAEVAISKQQGLSVSTRLKEVETV
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHCCCCHHHHHHHHEEE
EFNKDGALGITVFRDGCKGSSSTSDLSPEAIRQAVKAADDIARYTSADPCNGLAEASLMA
EECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KTVADLDLYHPESLTPEELTELAIRAEAAALDADPRIKNSDGASANAHTGIKVYGNSHGF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCC
LNGYCSSRYSLSCVVIGEEDDGNMQRDYDYSISRKYAELLSPELVGQKAAAKTLSRLGGR
EEEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCHHHHCHHHHHHHHHHHCCC
KIATGTMPVLFAHEVATGLIGHLISAISGSSLYRKSSFLLDGLDTQVFPEWLNIREEPHL
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHH
LAGLASAPYDSEGVATVDRDIIKDGLLATYLLTSYSARKLGMTNTGHAGGIYNWTLNDTG
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEECCCCC
VDFDALVRQMDKGLIVTEVMGQGVNMVTGDYSRGAAGFYVENGEIQYPVEEITIAGNLKD
CCHHHHHHHHHCCEEEEHHHCCCCEEEECCCCCCCCEEEEECCEEECCHHHEEEECCHHH
MYRSIQAVSQDRDLRSSIRTGGILLDSMKVAGN
HHHHHHHHHHHHHHHHHHHCCCEEEECEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]