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The map label for this gene is dut

Identifier: 119773466

GI number: 119773466

Start: 392676

End: 393134

Strand: Reverse

Name: dut

Synonym: Sama_0325

Alternate gene names: 119773466

Gene position: 393134-392676 (Counterclockwise)

Preceding gene: 119773467

Following gene: 119773465

Centisome position: 9.13

GC content: 58.39

Gene sequence:

>459_bases
ATGAAGACACCGATCGAACTGAAAATTCTCGACTCCCGCATAGGCTCGGAATTCCCACTGCCGGCCTATGCCACGCCTGG
CAGTGCCGGCATGGACCTTAGAGCCATGTTGGATACCAACCTGACCATAGCCCCTGGCGAAACCGTGCTCATACCTACCG
GCATCGCCGTGCATGTGGCCGACCCCAGCCTGGCAGCCGTGATCCTGCCCCGCTCCGGTATGGGCCATAAACATGGCATA
GTACTGGGCAACCTGGTGGGACTGATTGACTCTGACTATCAGGGTCCGCTGATGGTGTCCTGCTGGAACCGCGGCAACGA
GCCCTACACCCTGCAAATCGGTGACCGCCTGGCACAACTGGTGTTTGTTCCCGTAGTGCAGGCTGAATTTAAACTGGTGG
ACGAGTTCGACACCTCAATGCGCGGCGAAGGTGGTTTTGGCCATTCAGGCACCCGCTAA

Upstream 100 bases:

>100_bases
GCGTCTTTTGGCCTCAGGGCAACCAGGATTTGCCGGCAACCGACAAGTTGTCACTGGCATACCAACTGCTTTCGCTGATA
TCGACAAAAATAACCAAAAA

Downstream 100 bases:

>100_bases
CGTTTGCATTTAGTGTGAAGGAATCTCAATGGCCGCCAAAGAAAAAATCAGCCGCCGCGAACATATTCTCCAGTGTCTGG
CTCAGATGCTGGAAACCAAT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MKTPIELKILDSRIGSEFPLPAYATPGSAGMDLRAMLDTNLTIAPGETVLIPTGIAVHVADPSLAAVILPRSGMGHKHGI
VLGNLVGLIDSDYQGPLMVSCWNRGNEPYTLQIGDRLAQLVFVPVVQAEFKLVDEFDTSMRGEGGFGHSGTR

Sequences:

>Translated_152_residues
MKTPIELKILDSRIGSEFPLPAYATPGSAGMDLRAMLDTNLTIAPGETVLIPTGIAVHVADPSLAAVILPRSGMGHKHGI
VLGNLVGLIDSDYQGPLMVSCWNRGNEPYTLQIGDRLAQLVFVPVVQAEFKLVDEFDTSMRGEGGFGHSGTR
>Mature_152_residues
MKTPIELKILDSRIGSEFPLPAYATPGSAGMDLRAMLDTNLTIAPGETVLIPTGIAVHVADPSLAAVILPRSGMGHKHGI
VLGNLVGLIDSDYQGPLMVSCWNRGNEPYTLQIGDRLAQLVFVPVVQAEFKLVDEFDTSMRGEGGFGHSGTR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=34.4827586206897, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI4503423, Length=145, Percent_Identity=34.4827586206897, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI70906441, Length=145, Percent_Identity=34.4827586206897, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=69.5945945945946, Blast_Score=228, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71988561, Length=154, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319729, Length=132, Percent_Identity=34.0909090909091, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583610, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921126, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_SHEAM (A1S2D0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926206.1
- ProteinModelPortal:   A1S2D0
- SMR:   A1S2D0
- STRING:   A1S2D0
- GeneID:   4602581
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0325
- NMPDR:   fig|326297.7.peg.310
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   A1S2D0
- ProtClustDB:   PRK00601
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16199; Mature: 16199

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: BINDING 84-84 BINDING 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPIELKILDSRIGSEFPLPAYATPGSAGMDLRAMLDTNLTIAPGETVLIPTGIAVHVA
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCEEEEECCEEEEEE
DPSLAAVILPRSGMGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRGNEPYTLQIGDRLAQL
CCCEEEEEEECCCCCCCCCEEEEHEEEEECCCCCCCEEEEEECCCCCCEEEEHHHHHHHH
VFVPVVQAEFKLVDEFDTSMRGEGGFGHSGTR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTPIELKILDSRIGSEFPLPAYATPGSAGMDLRAMLDTNLTIAPGETVLIPTGIAVHVA
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCEEEEECCEEEEEE
DPSLAAVILPRSGMGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRGNEPYTLQIGDRLAQL
CCCEEEEEEECCCCCCCCCEEEEHEEEEECCCCCCCEEEEEECCCCCCEEEEHHHHHHHH
VFVPVVQAEFKLVDEFDTSMRGEGGFGHSGTR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA