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The map label for this gene is spoT [H]

Identifier: 119773405

GI number: 119773405

Start: 302833

End: 304932

Strand: Reverse

Name: spoT [H]

Synonym: Sama_0264

Alternate gene names: 119773405

Gene position: 304932-302833 (Counterclockwise)

Preceding gene: 119773406

Following gene: 119773404

Centisome position: 7.08

GC content: 55.05

Gene sequence:

>2100_bases
TTGTATCTGTTTGAAGGTTTAAAGGAATCGGCTTCCGCTTACCTCGACAGCGGCCAAGTGGCATTACTCAAACAGGCTTA
TCAGGTCGCCCGCGATGCCCATGAAGGGCAGATGCGCACCAGCGGCGAACCTTACATCACCCACCCGGTTGCTGTAGCCA
AGATTCTGTCCGACATGCGCCTCGACCACGAGACGCTGATGGCAGCCCTGCTGCACGACACCATTGAAGATACCCACGTC
ACCAAGGAAGATCTCGCAGAGCTGTTTGGCGATGCGGTGGCCGATCTGGTTGAAGGGGTGTCGAAGCTCGATAAGCTCAA
ATTCCGCGATAAAAAAGAAGCCCAGGCGGAAAACTTCCGCAAGATGATGATGGCCATGACCCAGGATATCCGGGTTATCC
TCATCAAGCTGGCTGACCGCACCCATAACATGCGCACACTCGGCGCTTTGCGTCCGGACAAACGCCGCCGCATCGCCCGG
GAAACCCTGGAAATTTACGCCCCCATTGCCAACCGCCTCGGTATTCACAATATCAAGACAGAGCTGGAGGATTTGGGCTT
TCAGGCGTATTACCCCATGCGCTACCGGGTGCTCAAAGAAGTGGTCAAGGCTGCCCGCGGTAACCGCAAAGAGCTTATTC
AAAACATTAAAACCGGCATCCAGGCACGACTGGAAGATGCAGGTATTCATGCCAAGGTCAAAGGCCGGGAGAAAAACCTT
TACTCCATCTACAACAAGATGCGCGGTAAAGAGTTACAGTTTCAGGAAGTCATGGATATCTATGCCTTCCGGGTCATAGT
AGATTCCATCGATACCTGCTATCGGGTGCTGGGGGCCATGCATGGCCTGTACAAGCCGCGTCCCGGTCGCTTCAAAGACT
ATATTGCCATTCCCAAGGCCAACGGCTATCAGTCGCTGCACACGTCTCTGTTTGGTCCCCACGGTGTACCGGTGGAAATT
CAAATCCGTACCGATGACATGGATCAAATGGCCGACAAAGGGGTTGCGGCCCACTGGGTATACAAAAACGGTAACGAAAA
CGGCAGCACCACCACTCAGGTGAGGGCCCGCAAATGGATGCAGAGCCTGCTGGAACTTCAGCAAAGCGCCAGCTCCTCCT
TCGAGTTTGTGGAAAACGTTAAAACCGAGCTCTTCCCCGACGAAATTTATGTGTTTACCCCCGAAGGCCGCATCCTCGAG
CTGCCGGTGGGTGCCACCGCGGTGGACTTTGCCTACGAGGTTCACACAGACGTGGGTAATACCTGCGTGGGTGCCCGGGT
AAACCGCCAGGCATATCCGCTCAGCCAACCACTAATCTCTGGCCAAACCGTTGAAATTATTACGGCAAAAGGCGCCAAGC
CCAATGCCGCCTGGCTCAACTTTGTGGTGACAGGAAAGGCGCGCGCCAAAATTCGTCAGGTACTGAAAAACCTCAAGCAG
GATGAAGCCATTGCACTGGGCCGCCGCTTGCTTAACCACGCCCTGGGTGAAAACAAACTCGACAGCGTGGCGCCGGAGCT
GCTCGATAAAGTTATTCAGGAAACCAAGCACAAGGATTTGAACAGCCTGCTGGCCGACATAGGTTTGGGCAATGCCATGA
GCATAGTCATCGCCCAGCGTCTGACCGGTGGCGCCGTGCCAGAAAAGCAAAGCGAAACCCATCTGATGCCAATCCGCGGC
GCCGAAGGCATGCTGGTGTCCTTCGCCAAGTGCTGCCACCCCATCCCGGGCGATGCGGTAATTGCCCACGTGAGCCCCGG
CAAGGGTCTGGTGGTACACATGGAAAACTGTGCCAACATTCGCGGCTATCAGGGCGAGCCTGACAAGTACATCCCTGTGC
ATTGGGATAAGGTTGACGGCGTGGAATACCAGGCCAACCTGAGGGTAGAAATCGTTAACCATCAGGGTGCGCTGGCCAAG
ATCACTTCCATTATCGCCAGCGAAGGGTCCAACATTCATAACCTCAGCACCGAAGAGCGTGATGGTAGGGTGTATTTGAT
TAACCTGCGGATTTCTGTGCGGGATCGTATTCACCTTGCCAACGTGATGCGCCGTATCCGGGTTCTGCCCGAGGTGCTGA
GAACCTCCCGCAATCGTTAG

Upstream 100 bases:

>100_bases
GCGTCAGAGCGTGCGCGAGCGCGAAGCGGCTGAAATCGCTGCCGTTGCCGCGATTGCAGAAGGTCGTTCTCTCTAATCCA
AGCCCCCGGAGGTATGCCAC

Downstream 100 bases:

>100_bases
TCATTTTCCTATAAGAGAGAACCCTATGGCTGAAAAAACCATTATCGCCACCGACAAGGCTCCTGCGGCTATCGGTACCT
ATTCCCAGGCCGTAAAGGTC

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MYLFEGLKESASAYLDSGQVALLKQAYQVARDAHEGQMRTSGEPYITHPVAVAKILSDMRLDHETLMAALLHDTIEDTHV
TKEDLAELFGDAVADLVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKTELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQNIKTGIQARLEDAGIHAKVKGREKNL
YSIYNKMRGKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTDDMDQMADKGVAAHWVYKNGNENGSTTTQVRARKWMQSLLELQQSASSSFEFVENVKTELFPDEIYVFTPEGRILE
LPVGATAVDFAYEVHTDVGNTCVGARVNRQAYPLSQPLISGQTVEIITAKGAKPNAAWLNFVVTGKARAKIRQVLKNLKQ
DEAIALGRRLLNHALGENKLDSVAPELLDKVIQETKHKDLNSLLADIGLGNAMSIVIAQRLTGGAVPEKQSETHLMPIRG
AEGMLVSFAKCCHPIPGDAVIAHVSPGKGLVVHMENCANIRGYQGEPDKYIPVHWDKVDGVEYQANLRVEIVNHQGALAK
ITSIIASEGSNIHNLSTEERDGRVYLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR

Sequences:

>Translated_699_residues
MYLFEGLKESASAYLDSGQVALLKQAYQVARDAHEGQMRTSGEPYITHPVAVAKILSDMRLDHETLMAALLHDTIEDTHV
TKEDLAELFGDAVADLVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKTELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQNIKTGIQARLEDAGIHAKVKGREKNL
YSIYNKMRGKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTDDMDQMADKGVAAHWVYKNGNENGSTTTQVRARKWMQSLLELQQSASSSFEFVENVKTELFPDEIYVFTPEGRILE
LPVGATAVDFAYEVHTDVGNTCVGARVNRQAYPLSQPLISGQTVEIITAKGAKPNAAWLNFVVTGKARAKIRQVLKNLKQ
DEAIALGRRLLNHALGENKLDSVAPELLDKVIQETKHKDLNSLLADIGLGNAMSIVIAQRLTGGAVPEKQSETHLMPIRG
AEGMLVSFAKCCHPIPGDAVIAHVSPGKGLVVHMENCANIRGYQGEPDKYIPVHWDKVDGVEYQANLRVEIVNHQGALAK
ITSIIASEGSNIHNLSTEERDGRVYLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR
>Mature_699_residues
MYLFEGLKESASAYLDSGQVALLKQAYQVARDAHEGQMRTSGEPYITHPVAVAKILSDMRLDHETLMAALLHDTIEDTHV
TKEDLAELFGDAVADLVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKTELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQNIKTGIQARLEDAGIHAKVKGREKNL
YSIYNKMRGKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTDDMDQMADKGVAAHWVYKNGNENGSTTTQVRARKWMQSLLELQQSASSSFEFVENVKTELFPDEIYVFTPEGRILE
LPVGATAVDFAYEVHTDVGNTCVGARVNRQAYPLSQPLISGQTVEIITAKGAKPNAAWLNFVVTGKARAKIRQVLKNLKQ
DEAIALGRRLLNHALGENKLDSVAPELLDKVIQETKHKDLNSLLADIGLGNAMSIVIAQRLTGGAVPEKQSETHLMPIRG
AEGMLVSFAKCCHPIPGDAVIAHVSPGKGLVVHMENCANIRGYQGEPDKYIPVHWDKVDGVEYQANLRVEIVNHQGALAK
ITSIIASEGSNIHNLSTEERDGRVYLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=703, Percent_Identity=69.9857752489332, Blast_Score=1035, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=701, Percent_Identity=32.6676176890157, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24650996, Length=144, Percent_Identity=35.4166666666667, Blast_Score=73, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 78399; Mature: 78399

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFEGLKESASAYLDSGQVALLKQAYQVARDAHEGQMRTSGEPYITHPVAVAKILSDMR
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHC
LDHETLMAALLHDTIEDTHVTKEDLAELFGDAVADLVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIYAPIANRLGIHNIKT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQNIKTGIQARLEDAGIHAKVKGREKNL
HHHHCCCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHH
YSIYNKMRGKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
NGYQSLHTSLFGPHGVPVEIQIRTDDMDQMADKGVAAHWVYKNGNENGSTTTQVRARKWM
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHH
QSLLELQQSASSSFEFVENVKTELFPDEIYVFTPEGRILELPVGATAVDFAYEVHTDVGN
HHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCHHHHHHEECCCCCC
TCVGARVNRQAYPLSQPLISGQTVEIITAKGAKPNAAWLNFVVTGKARAKIRQVLKNLKQ
CEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHCCH
DEAIALGRRLLNHALGENKLDSVAPELLDKVIQETKHKDLNSLLADIGLGNAMSIVIAQR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LTGGAVPEKQSETHLMPIRGAEGMLVSFAKCCHPIPGDAVIAHVSPGKGLVVHMENCANI
HCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCC
RGYQGEPDKYIPVHWDKVDGVEYQANLRVEIVNHQGALAKITSIIASEGSNIHNLSTEER
CCCCCCCCCCCCEEECCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCC
DGRVYLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR
CCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYLFEGLKESASAYLDSGQVALLKQAYQVARDAHEGQMRTSGEPYITHPVAVAKILSDMR
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHC
LDHETLMAALLHDTIEDTHVTKEDLAELFGDAVADLVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIYAPIANRLGIHNIKT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQNIKTGIQARLEDAGIHAKVKGREKNL
HHHHCCCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHH
YSIYNKMRGKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
NGYQSLHTSLFGPHGVPVEIQIRTDDMDQMADKGVAAHWVYKNGNENGSTTTQVRARKWM
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHH
QSLLELQQSASSSFEFVENVKTELFPDEIYVFTPEGRILELPVGATAVDFAYEVHTDVGN
HHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCHHHHHHEECCCCCC
TCVGARVNRQAYPLSQPLISGQTVEIITAKGAKPNAAWLNFVVTGKARAKIRQVLKNLKQ
CEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHCCH
DEAIALGRRLLNHALGENKLDSVAPELLDKVIQETKHKDLNSLLADIGLGNAMSIVIAQR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LTGGAVPEKQSETHLMPIRGAEGMLVSFAKCCHPIPGDAVIAHVSPGKGLVVHMENCANI
HCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCC
RGYQGEPDKYIPVHWDKVDGVEYQANLRVEIVNHQGALAKITSIIASEGSNIHNLSTEER
CCCCCCCCCCCCEEECCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCC
DGRVYLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR
CCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]