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The map label for this gene is trmA

Identifier: 119773334

GI number: 119773334

Start: 221311

End: 222408

Strand: Reverse

Name: trmA

Synonym: Sama_0193

Alternate gene names: 119773334

Gene position: 222408-221311 (Counterclockwise)

Preceding gene: 119773336

Following gene: 119773332

Centisome position: 5.16

GC content: 51.09

Gene sequence:

>1098_bases
ATGAACGCACAAGCCATGGACCCTCAACAGTACGAGCAGCAGCTTGAGCAAAAGTGCCAGGCACTGACTGAGGCATTTGC
CCACTATAATCCACCGGCTCTGGAAGTATTCCCATCTGCCCCAGCCCATTATCGGATGCGCTGTGAATTCCGGGTGTGGC
ACGATGGCGACGACCTCTACTACTGTATGTTCGACAACGTGGCAAAAGAAAAGGTACGTACCGATCAGTTTTTGCCAGCC
AGCGAACTCATCAACCGCATGATGCCTGCCCTGCTGGACGAACTTCGCCCCAATCGCGCCCTGCGCCACAAGCTGTTCCA
GGTAGACTTCTTGTCTACCCTGAGTGGCGAGATTCTGGTCAGTCTCTTGTATCACCGCCAGTTGGACGATCAATGGCTCA
CTGAAGCCCGTGCGCTCAAGGCGCGTCTGGGCGAGCACTTTAAAGTCGACATTATTGGCCGTGCCCGCAAACAAAAATTT
GTGCTCGACCGCGACTTTGTAGTTGAGTCACTCGATGTGGATGGTAAGACCCTGCACTATAAGCAGGTAGAGAACAGCTT
TACTCAGCCCAATGCAGGTGTGGCGGTTAAGATGTTGGAATGGGCGCTGGATGCCACCAAACAAAGTAGTGGTGACTTGC
TGGAGCTTTACTGTGGTAACGGCAACTTTTCCATCGCTTTGGCACCAAACTTTGGCAAGGTACTGGCGACTGAACTGGCC
AAACCCTCTGTAGAAGCGGCTCAGTACAATATTCAAGTCAATAAGGTAAGTAATCTCGATATCGTCCGTATGTCAGCGGA
AGAATTCACCGAAGCCATGAAAGGTGAAAAGCGTTTCAACCGTCTTGGCGACATTGATTTACAAAGCTACCAGTGCAACA
CCATCTTTGTGGATCCACCCAGAGCGGGCCTGGATGATGAGACCGTTAAGCTGGTTCAGGGTTACGAGAACATTCTCTAT
ATCTCCTGCAATCCCGATACTCTGAATGATAACCTCAAGGTACTTAGCGAAACTCACGAAGTAGTGCGCTTTGCGCTGTT
CGATCAGTTCCCTTATACCCATCACACAGAAGCCGGCGTGATGCTTAAACGCCGCTGA

Upstream 100 bases:

>100_bases
TTTCCCACAGCCTAAAATAAAGGGCAAAGCCAGAAAGCTGGACATACGGTTTCGCTGGGGTAAAATTTGCGGCCATTTTT
TAAGCCGGACGAGATTGCAA

Downstream 100 bases:

>100_bases
TACTGGCAATCATTACATAAAAAACGGAGCCTTAGGCTCCGTTTTTTATTTTGCATCGACCTTTTGCTGCAAAAACTCAG
CCCCTTTGGCCACCATACGC

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNAQAMDPQQYEQQLEQKCQALTEAFAHYNPPALEVFPSAPAHYRMRCEFRVWHDGDDLYYCMFDNVAKEKVRTDQFLPA
SELINRMMPALLDELRPNRALRHKLFQVDFLSTLSGEILVSLLYHRQLDDQWLTEARALKARLGEHFKVDIIGRARKQKF
VLDRDFVVESLDVDGKTLHYKQVENSFTQPNAGVAVKMLEWALDATKQSSGDLLELYCGNGNFSIALAPNFGKVLATELA
KPSVEAAQYNIQVNKVSNLDIVRMSAEEFTEAMKGEKRFNRLGDIDLQSYQCNTIFVDPPRAGLDDETVKLVQGYENILY
ISCNPDTLNDNLKVLSETHEVVRFALFDQFPYTHHTEAGVMLKRR

Sequences:

>Translated_365_residues
MNAQAMDPQQYEQQLEQKCQALTEAFAHYNPPALEVFPSAPAHYRMRCEFRVWHDGDDLYYCMFDNVAKEKVRTDQFLPA
SELINRMMPALLDELRPNRALRHKLFQVDFLSTLSGEILVSLLYHRQLDDQWLTEARALKARLGEHFKVDIIGRARKQKF
VLDRDFVVESLDVDGKTLHYKQVENSFTQPNAGVAVKMLEWALDATKQSSGDLLELYCGNGNFSIALAPNFGKVLATELA
KPSVEAAQYNIQVNKVSNLDIVRMSAEEFTEAMKGEKRFNRLGDIDLQSYQCNTIFVDPPRAGLDDETVKLVQGYENILY
ISCNPDTLNDNLKVLSETHEVVRFALFDQFPYTHHTEAGVMLKRR
>Mature_365_residues
MNAQAMDPQQYEQQLEQKCQALTEAFAHYNPPALEVFPSAPAHYRMRCEFRVWHDGDDLYYCMFDNVAKEKVRTDQFLPA
SELINRMMPALLDELRPNRALRHKLFQVDFLSTLSGEILVSLLYHRQLDDQWLTEARALKARLGEHFKVDIIGRARKQKF
VLDRDFVVESLDVDGKTLHYKQVENSFTQPNAGVAVKMLEWALDATKQSSGDLLELYCGNGNFSIALAPNFGKVLATELA
KPSVEAAQYNIQVNKVSNLDIVRMSAEEFTEAMKGEKRFNRLGDIDLQSYQCNTIFVDPPRAGLDDETVKLVQGYENILY
ISCNPDTLNDNLKVLSETHEVVRFALFDQFPYTHHTEAGVMLKRR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=200, Percent_Identity=27, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI51173878, Length=200, Percent_Identity=27, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790403, Length=363, Percent_Identity=60.0550964187328, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1789148, Length=187, Percent_Identity=32.620320855615, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24666579, Length=196, Percent_Identity=27.0408163265306, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_SHEAM (A1S1Z8)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926074.1
- ProteinModelPortal:   A1S1Z8
- SMR:   A1S1Z8
- STRING:   A1S1Z8
- GeneID:   4602449
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0193
- NMPDR:   fig|326297.7.peg.183
- eggNOG:   COG2265
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- PhylomeDB:   A1S1Z8
- ProtClustDB:   PRK05031
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 41849; Mature: 41849

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 323-323 BINDING 189-189 BINDING 217-217 BINDING 222-222 BINDING 238-238 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQAMDPQQYEQQLEQKCQALTEAFAHYNPPALEVFPSAPAHYRMRCEFRVWHDGDDLY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEEEEEECCCCEE
YCMFDNVAKEKVRTDQFLPASELINRMMPALLDELRPNRALRHKLFQVDFLSTLSGEILV
EEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDDQWLTEARALKARLGEHFKVDIIGRARKQKFVLDRDFVVESLDVDGKTLHY
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHEEECHHHHHHCCCCCCCEEEH
KQVENSFTQPNAGVAVKMLEWALDATKQSSGDLLELYCGNGNFSIALAPNFGKVLATELA
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHC
KPSVEAAQYNIQVNKVSNLDIVRMSAEEFTEAMKGEKRFNRLGDIDLQSYQCNTIFVDPP
CCCCCCEEEEEEEEEECCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEECCEEEECCC
RAGLDDETVKLVQGYENILYISCNPDTLNDNLKVLSETHEVVRFALFDQFPYTHHTEAGV
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
MLKRR
EEECC
>Mature Secondary Structure
MNAQAMDPQQYEQQLEQKCQALTEAFAHYNPPALEVFPSAPAHYRMRCEFRVWHDGDDLY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEEEEEECCCCEE
YCMFDNVAKEKVRTDQFLPASELINRMMPALLDELRPNRALRHKLFQVDFLSTLSGEILV
EEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDDQWLTEARALKARLGEHFKVDIIGRARKQKFVLDRDFVVESLDVDGKTLHY
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHEEECHHHHHHCCCCCCCEEEH
KQVENSFTQPNAGVAVKMLEWALDATKQSSGDLLELYCGNGNFSIALAPNFGKVLATELA
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHC
KPSVEAAQYNIQVNKVSNLDIVRMSAEEFTEAMKGEKRFNRLGDIDLQSYQCNTIFVDPP
CCCCCCEEEEEEEEEECCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEECCEEEECCC
RAGLDDETVKLVQGYENILYISCNPDTLNDNLKVLSETHEVVRFALFDQFPYTHHTEAGV
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
MLKRR
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA