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The map label for this gene is menF [H]

Identifier: 119773272

GI number: 119773272

Start: 145079

End: 146437

Strand: Reverse

Name: menF [H]

Synonym: Sama_0130

Alternate gene names: 119773272

Gene position: 146437-145079 (Counterclockwise)

Preceding gene: 119773279

Following gene: 119773270

Centisome position: 3.4

GC content: 55.26

Gene sequence:

>1359_bases
TTGTCAGTCATGTCCCTGCCCGATGCCTTGCTGTCACTCGAGGCAAAGATTCAACATCTCAGTACAACCCAGGGCAGTGA
GCCCTTGGTGCAGCTGACAGAGGCAGTCCCTGCCTTGCCCTTGATATCCTGGCTGGCTTCTCAGGAGCTGTATCCCAGGA
TCTATTGGCATGGCCGTGATAAAGAAGAAGAGGTGGCTGCCATCGGCTGCACCTGTGATTTTTTCTTCGATGACCAAGTG
GCTGATCAGCAGTTGGCGGAGGTTTATCAACGTCAGCGCGCTTTGAGCTATAACCAAAATTTCCGTTATTACGGTGGTGT
AGCCTTTGACCGTGAGGTAGAAGGCTGGCCGGAGTTTGGCCGTGCCCGTTTTGTACTGCCCCGTATTGAGCTGCGCCGCA
GCGGCAAGGAATTCAAACTGCTGCTTAACCTGAATTTTGATGGCAACGACAGGCATGCAGAGTGTCAGGCCGCACTGCAG
GCGCTGAGTTCGCTAAAGCGTCCCAAGCCCCTGACACCACCCGGTAAGGTAAATCTACTGGGGCGCAGTGAATATCCCGA
ATATCCCCGCTGGCAACAGTTGGTGGAGCAGGTGACGGCGGATAAATTTAACCGGCACACTCCCAAGGTGGTGCTCTCCC
GGTTGTCACAACTGGAAATCAATGACCCGGTCGATCCCTGGATGGTACTGGCCTGCTGGCAGGGCCGCTGTCCAAGCAGC
TTCCAGTTTGGATTTCAGTTTTCCAAAGACAGAACCTTTATTTCCTGCTCGCCCGAGCGGTTGTATCTAAGGCGGGGACA
AGAGCTCTTCACCGAGGCGCTGGCGGGCACCACGCTGCGCGGCCATACCGAGGAAGAAGATGCTCTGCTTGCAAAACAAT
TACAGGAAGACAGTAAAAACAGTCACGAGAATCAGCTGGTTCGCCAGCACATAGTCGAGCACCTGTCCCCTCTGAGCCAC
TATGTGGGTGCCGAAGAGTCGCCACGGATCTTTAAGCTGCCCCATATCCAGCATTTGCACCGTGCCATTCGTGCCGAGCT
GAAGCCCGGCATCAATGATTTTCAGCTATTGCTGGCTTTGCATCCCACTCCAGCCGTTGGCGGTCTGCCAAGGCCCAGCG
CCTTGAGCTTCATTCGCCAACAGGAAGGATATAACCGGGGGTGGTACGCAGGTGCATGCGGTTACCTGAATCGCCATGAG
AGTGAGTTCTCCGTGGCCATTCGCAGTGCGCTGATTGAGCCGGGACGAATTAACCTGTTTGCCGGTGCCGGCATAGTGGC
AGGTTCAGACCCCGAAGCAGAATGGCAGGAGCTGGACAATAAGCTCGCCACCATCTTATCGATTCTGGTGGAGCTCTGA

Upstream 100 bases:

>100_bases
ACAGCTTGGGTTCTGGACGATTGACACCGGCACTCATGCTATTTAACGTTGGGTAACAACAATAAAAGCAGTGTGTTGTA
TTACGACCGGAGACCCTTTT

Downstream 100 bases:

>100_bases
CGTCTTAACAAATCACCTAGAGGTTTGCATGCTGTGATTGCGTGCCTTGAAGCCTGACCCGCTATGGGATAAGGCCGAGT
TTGCGCTGGGTAGCCTCAAA

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSVMSLPDALLSLEAKIQHLSTTQGSEPLVQLTEAVPALPLISWLASQELYPRIYWHGRDKEEEVAAIGCTCDFFFDDQV
ADQQLAEVYQRQRALSYNQNFRYYGGVAFDREVEGWPEFGRARFVLPRIELRRSGKEFKLLLNLNFDGNDRHAECQAALQ
ALSSLKRPKPLTPPGKVNLLGRSEYPEYPRWQQLVEQVTADKFNRHTPKVVLSRLSQLEINDPVDPWMVLACWQGRCPSS
FQFGFQFSKDRTFISCSPERLYLRRGQELFTEALAGTTLRGHTEEEDALLAKQLQEDSKNSHENQLVRQHIVEHLSPLSH
YVGAEESPRIFKLPHIQHLHRAIRAELKPGINDFQLLLALHPTPAVGGLPRPSALSFIRQQEGYNRGWYAGACGYLNRHE
SEFSVAIRSALIEPGRINLFAGAGIVAGSDPEAEWQELDNKLATILSILVEL

Sequences:

>Translated_452_residues
MSVMSLPDALLSLEAKIQHLSTTQGSEPLVQLTEAVPALPLISWLASQELYPRIYWHGRDKEEEVAAIGCTCDFFFDDQV
ADQQLAEVYQRQRALSYNQNFRYYGGVAFDREVEGWPEFGRARFVLPRIELRRSGKEFKLLLNLNFDGNDRHAECQAALQ
ALSSLKRPKPLTPPGKVNLLGRSEYPEYPRWQQLVEQVTADKFNRHTPKVVLSRLSQLEINDPVDPWMVLACWQGRCPSS
FQFGFQFSKDRTFISCSPERLYLRRGQELFTEALAGTTLRGHTEEEDALLAKQLQEDSKNSHENQLVRQHIVEHLSPLSH
YVGAEESPRIFKLPHIQHLHRAIRAELKPGINDFQLLLALHPTPAVGGLPRPSALSFIRQQEGYNRGWYAGACGYLNRHE
SEFSVAIRSALIEPGRINLFAGAGIVAGSDPEAEWQELDNKLATILSILVEL
>Mature_451_residues
SVMSLPDALLSLEAKIQHLSTTQGSEPLVQLTEAVPALPLISWLASQELYPRIYWHGRDKEEEVAAIGCTCDFFFDDQVA
DQQLAEVYQRQRALSYNQNFRYYGGVAFDREVEGWPEFGRARFVLPRIELRRSGKEFKLLLNLNFDGNDRHAECQAALQA
LSSLKRPKPLTPPGKVNLLGRSEYPEYPRWQQLVEQVTADKFNRHTPKVVLSRLSQLEINDPVDPWMVLACWQGRCPSSF
QFGFQFSKDRTFISCSPERLYLRRGQELFTEALAGTTLRGHTEEEDALLAKQLQEDSKNSHENQLVRQHIVEHLSPLSHY
VGAEESPRIFKLPHIQHLHRAIRAELKPGINDFQLLLALHPTPAVGGLPRPSALSFIRQQEGYNRGWYAGACGYLNRHES
EFSVAIRSALIEPGRINLFAGAGIVAGSDPEAEWQELDNKLATILSILVEL

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=443, Percent_Identity=35.4401805869075, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1786809, Length=275, Percent_Identity=30.1818181818182, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787518, Length=251, Percent_Identity=28.2868525896414, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788114, Length=201, Percent_Identity=30.8457711442786, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320935, Length=337, Percent_Identity=27.299703264095, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 51293; Mature: 51162

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVMSLPDALLSLEAKIQHLSTTQGSEPLVQLTEAVPALPLISWLASQELYPRIYWHGRD
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
KEEEVAAIGCTCDFFFDDQVADQQLAEVYQRQRALSYNQNFRYYGGVAFDREVEGWPEFG
CCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCCCCCCCCCC
RARFVLPRIELRRSGKEFKLLLNLNFDGNDRHAECQAALQALSSLKRPKPLTPPGKVNLL
CCEEEECHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
GRSEYPEYPRWQQLVEQVTADKFNRHTPKVVLSRLSQLEINDPVDPWMVLACWQGRCPSS
ECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCC
FQFGFQFSKDRTFISCSPERLYLRRGQELFTEALAGTTLRGHTEEEDALLAKQLQEDSKN
CCCCEEECCCCEEEECCHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
SHENQLVRQHIVEHLSPLSHYVGAEESPRIFKLPHIQHLHRAIRAELKPGINDFQLLLAL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCEEEEEEE
HPTPAVGGLPRPSALSFIRQQEGYNRGWYAGACGYLNRHESEFSVAIRSALIEPGRINLF
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCEEEE
AGAGIVAGSDPEAEWQELDNKLATILSILVEL
ECCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVMSLPDALLSLEAKIQHLSTTQGSEPLVQLTEAVPALPLISWLASQELYPRIYWHGRD
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
KEEEVAAIGCTCDFFFDDQVADQQLAEVYQRQRALSYNQNFRYYGGVAFDREVEGWPEFG
CCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCCCCCCCCCC
RARFVLPRIELRRSGKEFKLLLNLNFDGNDRHAECQAALQALSSLKRPKPLTPPGKVNLL
CCEEEECHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
GRSEYPEYPRWQQLVEQVTADKFNRHTPKVVLSRLSQLEINDPVDPWMVLACWQGRCPSS
ECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCC
FQFGFQFSKDRTFISCSPERLYLRRGQELFTEALAGTTLRGHTEEEDALLAKQLQEDSKN
CCCCEEECCCCEEEECCHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
SHENQLVRQHIVEHLSPLSHYVGAEESPRIFKLPHIQHLHRAIRAELKPGINDFQLLLAL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCEEEEEEE
HPTPAVGGLPRPSALSFIRQQEGYNRGWYAGACGYLNRHESEFSVAIRSALIEPGRINLF
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCEEEE
AGAGIVAGSDPEAEWQELDNKLATILSILVEL
ECCCEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]